Chorion-specific transcription factor GCMa

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P70348 (GCM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chorion-specific transcription factor GCMa

Alternative name(s):
GCM motif protein 1
Short name=mGCM1
Short name=mGCMa
Glial cells missing homolog 1

Gene names

Name:	Gcm1
Synonyms:	Gcma

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that is necessary for placental development By similarity. Ref.2
Subcellular location	Nucleus Potential.
Tissue specificity	Placenta specific. Ref.1
Post-translational modification	Polyubiquitinated in the presence of UBE2D2 and FBXW2 (in vitro) By similarity.
Sequence similarities	Contains 1 GCM DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	astrocyte fate commitment Inferred from direct assay. Source: MGI branching involved in embryonic placenta morphogenesis Inferred from mutant phenotype. Source: MGI cell differentiation involved in embryonic placenta development Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from direct assay. Source: MGI sequence-specific DNA binding transcription factor activity Traceable author statement. Source: MGI zinc ion binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 436

436

Chorion-specific transcription factor GCMa

PRO_0000126648

Regions

DNA binding

14 – 169

156

GCM

Sites

Metal binding

Zinc 1

Metal binding

Zinc 2

Metal binding

Zinc 2

Metal binding

113

Zinc 2

Metal binding

116

Zinc 2

Metal binding

125

Zinc 1

Metal binding

152

Zinc 1

Metal binding

154

Zinc 1

Experimental info

Mutagenesis

N → A: No effect on DNA binding. Ref.4

Mutagenesis

N → Q: Slight decrease of DNA binding. Ref.4

Mutagenesis

N → A: Strong decrease of DNA binding. Ref.4

Mutagenesis

N → D: Loss of DNA binding. Ref.4

Mutagenesis

K → A, L or M: Loss of DNA binding. Ref.4

Mutagenesis

C → A: Loss of transcription activation. Ref.4

Mutagenesis

C → A: Loss of transcription activation. Ref.4

Mutagenesis

113

C → A: Loss of transcription activation. Ref.4

Mutagenesis

125

C → A: Loss of transcription activation. Ref.4

Mutagenesis

152

H → A: Loss of transcription activation. Ref.4

Mutagenesis

154

H → A: Loss of transcription activation. Ref.4

Sequence conflict

211

G → S in BAA13650. Ref.2

Sequence conflict

230

N → K in BAA13650. Ref.2

Sequence conflict

241

S → N in BAA13650. Ref.2

Sequence conflict

413

D → E in BAA13650. Ref.2

Secondary structure

436

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P70348 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 99F4E441800582DB
Show »

FASTA

436

49,589

        10         20         30         40         50         60 
MELDDFDPED KEILSWDIND VKLPQNVKTT DWFQEWPDSY VKHIYSSDDR NAQRHLSSWA 

        70         80         90        100        110        120 
MRNTNNHNSR ILKKSCLGVV VCSRDCSTEE GRKIYLRPAI CDKARQKQQR KSCPNCNGPL 

       130        140        150        160        170        180 
KLIPCRGHGG FPVTNFWRHD GRFIFFQSKG EHDHPRPETK LEAEARRAMK KVHMASASNS 

       190        200        210        220        230        240 
LRMKGRPAAK ALPAEIPSQG SLPLTWSFQE GVQLPGTYST PLIANAPQQN SLNDCLSFPK 

       250        260        270        280        290        300 
SYDLGGSTEL EDPTSTLDSM KFYERCKFSS SRIYGSEEQF QPPVPGTYGD YEDLQTWNKN 

       310        320        330        340        350        360 
VALGRNPSDD IYYPAYPLPV ASWPYDYFPS QNSLEHLPQQ VPSEPPAAQP GCHPLWSNPG 

       370        380        390        400        410        420 
GEPYEEKVSV DLSSYVPSLT YHPPQQDPFL LTYGSPTQQQ HALPGKSNRW DFDEEMACMG 

       430 
LDHFNNEMLL NFCSLR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gcm1, a mammalian homolog of Drosophila glial cells missing." Altshuller Y., Copeland N.G., Gilbert D.J., Jenkins N.A., Frohman M.A. FEBS Lett. 393:201-204(1996) [PubMed: 8814290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[2]	"The gcm-motif: a novel DNA binding motif conserved in Drosophila and mammals." Akiyama Y., Hosoya T., Poole A.M., Hotta Y. Proc. Natl. Acad. Sci. U.S.A. 93:14912-14916(1996) [PubMed: 8962155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[4]	"Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition." Cohen S.X., Moulin M., Hashemolhosseini S., Kilian K., Wegner M., Mueller C.W. EMBO J. 22:1835-1845(2003) [PubMed: 12682016] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 1-174 IN COMPLEX WITH DNA AND ZINC IONS, MUTAGENESIS OF ASN-63; ASN-65; LYS-74; CYS-76; CYS-82; CYS-113; CYS-125; HIS-152 AND HIS-154.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U59876 mRNA. Translation: AAC52822.1.
D88612 mRNA. Translation: BAA13650.1.
BC066866 mRNA. Translation: AAH66866.1.

IPI

IPI00307860.

PIR

S74257.

RefSeq

NP_032129.2. NM_008103.3.

UniGene

Mm.1400.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ODH	X-ray	2.85	A	1-174	[»]

ProteinModelPortal

P70348.

SMR

P70348. Positions 14-170.

ModBase

Search...

Protein-protein interaction databases

STRING

P70348.

PTM databases

PhosphoSite

P70348.

Proteomic databases

PRIDE

P70348.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000024104; ENSMUSP00000024104; ENSMUSG00000023333.

GeneID

14531.

KEGG

mmu:14531.

Organism-specific databases

CTD

8521.

MGI

MGI:108045. Gcm1.

Phylogenomic databases

eggNOG

roNOG15196.

HOGENOM

HBG267490.

HOVERGEN

HBG039460.

InParanoid

P70348.

OMA

EKVHVDF.

OrthoDB

EOG43XV39.

PhylomeDB

P70348.

Gene expression databases

ArrayExpress

P70348.

Bgee

P70348.

CleanEx

MM_GCM1.

Genevestigator

P70348.

GermOnline

ENSMUSG00000023333. Mus musculus.

Family and domain databases

InterPro

IPR003902. Tscrpt_reg_GCM_motif.
[Graphical view]

PANTHER

PTHR12414. GCM_motif. 1 hit.

Pfam

PF03615. GCM. 1 hit.
[Graphical view]

ProDom

PD014393. GCM_motif. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF90073. GCM_motif. 1 hit.

PROSITE

PS50807. GCM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

286180.

SOURCE

Search...

Entry information

Entry name

GCM1_MOUSE

Accession

Primary (citable) accession number: P70348
Secondary accession number(s): O09103

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	February 1, 1997
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents