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Protein

Chorion-specific transcription factor GCMa

Gene

Gcm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is necessary for placental development (PubMed:8962155). Involved in the control of expression of placental growth factor (PGF) and other placenta-specific genes. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer. Binds to the SYDE1 promoter. Has a central role in mediating the differentiation of trophoblast cells along both the villous and extravillous pathways in placental development (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Zinc 11
Metal bindingi82Zinc 21
Metal bindingi86Zinc 21
Metal bindingi113Zinc 21
Metal bindingi116Zinc 21
Metal bindingi125Zinc 11
Metal bindingi152Zinc 11
Metal bindingi154Zinc 11

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi14 – 169GCMPROSITE-ProRule annotationAdd BLAST156

GO - Molecular functioni

GO - Biological processi

  • astrocyte fate commitment Source: MGI
  • branching involved in labyrinthine layer morphogenesis Source: MGI
  • cell differentiation involved in embryonic placenta development Source: MGI
  • positive regulation of syncytium formation by plasma membrane fusion Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of cell differentiation involved in embryonic placenta development Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chorion-specific transcription factor GCMa
Alternative name(s):
GCM motif protein 1
Short name:
mGCM1
Short name:
mGCMa
Glial cells missing homolog 1
Gene namesi
Name:Gcm1
Synonyms:Gcma
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:108045. Gcm1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63N → A: No effect on DNA binding. 1 Publication1
Mutagenesisi63N → Q: Slight decrease of DNA binding. 1 Publication1
Mutagenesisi65N → A: Strong decrease of DNA binding. 1 Publication1
Mutagenesisi65N → D: Loss of DNA binding. 1 Publication1
Mutagenesisi74K → A, L or M: Loss of DNA binding. 1 Publication1
Mutagenesisi76C → A: Loss of transcription activation. 1 Publication1
Mutagenesisi82C → A: Loss of transcription activation. 1 Publication1
Mutagenesisi113C → A: Loss of transcription activation. 1 Publication1
Mutagenesisi125C → A: Loss of transcription activation. 1 Publication1
Mutagenesisi152H → A: Loss of transcription activation. 1 Publication1
Mutagenesisi154H → A: Loss of transcription activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266481 – 436Chorion-specific transcription factor GCMaAdd BLAST436

Post-translational modificationi

Polyubiquitinated in the presence of UBE2D2 and FBXW2 (in vitro).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP70348.
PRIDEiP70348.

PTM databases

iPTMnetiP70348.
PhosphoSitePlusiP70348.

Expressioni

Tissue specificityi

Placenta specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000023333.
CleanExiMM_GCM1.
ExpressionAtlasiP70348. baseline and differential.
GenevisibleiP70348. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024104.

Structurei

Secondary structure

1436
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi50 – 53Combined sources4
Beta strandi61 – 64Combined sources4
Beta strandi72 – 76Combined sources5
Beta strandi79 – 82Combined sources4
Beta strandi89 – 91Combined sources3
Helixi102 – 110Combined sources9
Beta strandi114 – 116Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi134 – 139Combined sources6
Beta strandi141 – 149Combined sources9
Helixi160 – 167Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ODHX-ray2.85A1-174[»]
ProteinModelPortaliP70348.
SMRiP70348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70348.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IKM6. Eukaryota.
ENOG4110IIJ. LUCA.
GeneTreeiENSGT00390000006777.
HOGENOMiHOG000112700.
HOVERGENiHBG039460.
InParanoidiP70348.
KOiK21598.
OMAiWDFDEEM.
OrthoDBiEOG091G06SW.
PhylomeDBiP70348.
TreeFamiTF324146.

Family and domain databases

InterProiView protein in InterPro
IPR003902. Tscrpt_reg_GCM.
PfamiView protein in Pfam
PF03615. GCM. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014393. GCM_motif. 1 hit.
SUPFAMiSSF90073. SSF90073. 1 hit.
PROSITEiView protein in PROSITE
PS50807. GCM. 1 hit.

Sequencei

Sequence statusi: Complete.

P70348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDDFDPED KEILSWDIND VKLPQNVKTT DWFQEWPDSY VKHIYSSDDR
60 70 80 90 100
NAQRHLSSWA MRNTNNHNSR ILKKSCLGVV VCSRDCSTEE GRKIYLRPAI
110 120 130 140 150
CDKARQKQQR KSCPNCNGPL KLIPCRGHGG FPVTNFWRHD GRFIFFQSKG
160 170 180 190 200
EHDHPRPETK LEAEARRAMK KVHMASASNS LRMKGRPAAK ALPAEIPSQG
210 220 230 240 250
SLPLTWSFQE GVQLPGTYST PLIANAPQQN SLNDCLSFPK SYDLGGSTEL
260 270 280 290 300
EDPTSTLDSM KFYERCKFSS SRIYGSEEQF QPPVPGTYGD YEDLQTWNKN
310 320 330 340 350
VALGRNPSDD IYYPAYPLPV ASWPYDYFPS QNSLEHLPQQ VPSEPPAAQP
360 370 380 390 400
GCHPLWSNPG GEPYEEKVSV DLSSYVPSLT YHPPQQDPFL LTYGSPTQQQ
410 420 430
HALPGKSNRW DFDEEMACMG LDHFNNEMLL NFCSLR
Length:436
Mass (Da):49,589
Last modified:February 1, 1997 - v1
Checksum:i99F4E441800582DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211G → S in BAA13650 (PubMed:8962155).Curated1
Sequence conflicti230N → K in BAA13650 (PubMed:8962155).Curated1
Sequence conflicti241S → N in BAA13650 (PubMed:8962155).Curated1
Sequence conflicti413D → E in BAA13650 (PubMed:8962155).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59876 mRNA. Translation: AAC52822.1.
D88612 mRNA. Translation: BAA13650.1.
BC066866 mRNA. Translation: AAH66866.1.
CCDSiCCDS23356.1.
PIRiS74257.
RefSeqiNP_032129.2. NM_008103.3.
UniGeneiMm.1400.

Genome annotation databases

EnsembliENSMUST00000024104; ENSMUSP00000024104; ENSMUSG00000023333.
GeneIDi14531.
KEGGimmu:14531.
UCSCiuc009qto.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiGCM1_MOUSE
AccessioniPrimary (citable) accession number: P70348
Secondary accession number(s): O09103
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: February 1, 1997
Last modified: September 27, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references