TRAF family member-associated NF-kappa-B activator

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P70347 (TANK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
TRAF family member-associated NF-kappa-B activator

Alternative name(s):
TRAF-interacting protein
Short name=I-TRAF

Gene names

Name:	Tank
Synonyms:	Itraf

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity.
Subunit structure	Interacts with TBK1 By similarity. Interacts also with TRAF1, TRAF2, and TRAF3 by binding to their TRAF-C domains. Interacts more strongly with TRAF1 and TRAF2 than TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG By similarity. Ref.3
Subcellular location	Cytoplasm.
Tissue specificity	Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Sequence similarities	Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	I-kappaB kinase/NF-kappaB cascade Inferred from direct assay Ref.1. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
Tbk1	Q9WUN2	4	EBI-646116,EBI-764193
Traf1	P39428	12	EBI-646125,EBI-520123
Traf2	P39429	7	EBI-646125,EBI-520016

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P70347-1) Also known as: Beta; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P70347-2) Also known as: Alpha; The sequence of this isoform differs from the canonical sequence as follows: 1-34: Missing.

Isoform 3 (identifier: P70347-3) Also known as: Gamma; The sequence of this isoform differs from the canonical sequence as follows: 238-251: GMSCVTAVTPRGLG → ERRVCQLETTMCSM 252-448: Missing.

Isoform 4 (identifier: P70347-4) The sequence of this isoform differs from the canonical sequence as follows: 391-395: PFWKP → VTVLH 396-448: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 448

448

TRAF family member-associated NF-kappa-B activator

PRO_0000072428

Regions

Zinc finger

417 – 443

C2H2-type

Region

205 – 224

TRAF family member interaction

Amino acid modifications

Modified residue

377

Phosphoserine By similarity

Modified residue

380

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 34

Missing in isoform 2.

VSP_004444

Alternative sequence

238 – 251

GMSCV…PRGLG → ERRVCQLETTMCSM in isoform 3.

VSP_004445

Alternative sequence

252 – 448

197

Missing in isoform 3.

VSP_004446

Alternative sequence

391 – 395

PFWKP → VTVLH in isoform 4.

VSP_004447

Alternative sequence

396 – 448

Missing in isoform 4.

VSP_004448

Experimental info

Sequence conflict

Missing in AAB02204. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (Beta) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 313E704C881FB5F3 Show »	FASTA	448	50,939
10 20 30 40 50 60 MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF RQACMDRDSA 70 80 90 100 110 120 VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV DSSRDNSYGY VPLLEDSDRR 130 140 150 160 170 180 KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV SSGKESAKGL NIPLHHERDN IEKTFWDLKE 190 200 210 220 230 240 EFHRICLLAK AQKDHLSKLN IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS 250 260 270 280 290 300 CVTAVTPRGL GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD 310 320 330 340 350 360 RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT CLPAGDHNVF 370 380 390 400 410 420 YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD TDLVVPSDSD SELLKPLVCE 430 440 FCQELFPPSI TSRGDFLRHL NTHFNGET « Hide
Isoform 2 (Alpha) [UniParc]. Checksum: BE53BC1B0915BF21 Show »	FASTA	414	47,147
10 20 30 40 50 60 MDKNIGEQLN RAYEAFRQAC MDRDSAVREL QQKQTENYEQ RIREQQEQLS FQQNLIDRLK 70 80 90 100 110 120 SQLLLVDSSR DNSYGYVPLL EDSDRRKNNL TLDEPHDKVK LGTLRDKQSK VRRQEVSSGK 130 140 150 160 170 180 ESAKGLNIPL HHERDNIEKT FWDLKEEFHR ICLLAKAQKD HLSKLNIPDI ATDTQCSVPI 190 200 210 220 230 240 QCTDKTEKQE ALFKPQAKDD INRGMSCVTA VTPRGLGRDE EDTSFESLSK FNVKFPPMDN 250 260 270 280 290 300 DSIFLHSTPE APSILAPATP ETVCQDRFNM EVRDNPGNFV KTEETLFEIQ GIDPITSAIQ 310 320 330 340 350 360 NLKTTDKTNP SNLRATCLPA GDHNVFYVNT FPLQDPPDAP FPSLDSPGKA VRGPQQPFWK 370 380 390 400 410 PFLNQDTDLV VPSDSDSELL KPLVCEFCQE LFPPSITSRG DFLRHLNTHF NGET « Hide
Isoform 3 (Gamma) [UniParc]. Checksum: DE48310FBE7188B9 Show »	FASTA	251	29,255
10 20 30 40 50 60 MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF RQACMDRDSA 70 80 90 100 110 120 VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV DSSRDNSYGY VPLLEDSDRR 130 140 150 160 170 180 KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV SSGKESAKGL NIPLHHERDN IEKTFWDLKE 190 200 210 220 230 240 EFHRICLLAK AQKDHLSKLN IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRERR 250 VCQLETTMCS M « Hide
Isoform 4 [UniParc]. Checksum: 0457CDB69425C0A0 Show »	FASTA	395	44,827
10 20 30 40 50 60 MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF RQACMDRDSA 70 80 90 100 110 120 VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV DSSRDNSYGY VPLLEDSDRR 130 140 150 160 170 180 KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV SSGKESAKGL NIPLHHERDN IEKTFWDLKE 190 200 210 220 230 240 EFHRICLLAK AQKDHLSKLN IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS 250 260 270 280 290 300 CVTAVTPRGL GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD 310 320 330 340 350 360 RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT CLPAGDHNVF 370 380 390 YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ VTVLH « Hide

References

[1]	"I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction." Rothe M., Xiong J., Shu H.-B., Williamson K., Goddard A., Goeddel D.V. Proc. Natl. Acad. Sci. U.S.A. 93:8241-8246(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]	"TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation." Cheng G., Baltimore D. Genes Dev. 10:963-973(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/Kaplan. Tissue: T-cell lymphoma.
[3]	"Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases." Chariot A., Leonardi A., Muller J., Bonif M., Brown K., Siebenlist U. J. Biol. Chem. 277:37029-37036(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IKBKG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59864 mRNA. Translation: AAC52781.1. U51907 mRNA. Translation: AAB02204.1.
IPI	IPI00108187. IPI00230207. IPI00230208. IPI00230209.
RefSeq	NP_001157544.1. NM_001164072.1. NP_035659.1. NM_011529.2.
UniGene	Mm.244393.
3D structure databases
ProteinModelPortal	P70347.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70347. 4 interactions.
PTM databases
PhosphoSite	P70347.
Proteomic databases
PRIDE	P70347.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289.
GeneID	21353.
KEGG	mmu:21353.
UCSC	uc008juw.2. mouse. uc008juz.2. mouse.
Organism-specific databases
CTD	10010.
MGI	MGI:107676. Tank.
Phylogenomic databases
eggNOG	NOG44252.
GeneTree	ENSGT00390000008712.
HOGENOM	HOG000231816.
HOVERGEN	HBG019299.
InParanoid	P70347.
KO	K12650.
OMA	ETQCSVP.
Gene expression databases
ArrayExpress	P70347.
Bgee	P70347.
Genevestigator	P70347.
GermOnline	ENSMUSG00000064289. Mus musculus.
Family and domain databases
InterPro	IPR024581. TBD. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF12845. TBD. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TANK. mouse.
NextBio	300548.
SOURCE	Search...

Entry information

Entry name

TANK_MOUSE

Accession

Primary (citable) accession number: P70347
Secondary accession number(s): Q61178

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents