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Protein

TRAF family member-associated NF-kappa-B activator

Gene

Tank

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri417 – 443C2H2-typeAdd BLAST27

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TRAF family member-associated NF-kappa-B activator
Alternative name(s):
TRAF-interacting protein
Short name:
I-TRAF
Gene namesi
Name:Tank
Synonyms:Itraf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107676. Tank.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000724281 – 448TRAF family member-associated NF-kappa-B activatorAdd BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei211PhosphoserineBy similarity1
Modified residuei246PhosphothreonineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei377PhosphoserineBy similarity1
Modified residuei380PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70347.
PaxDbiP70347.
PRIDEiP70347.

PTM databases

iPTMnetiP70347.
PhosphoSitePlusiP70347.

Expressioni

Tissue specificityi

Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Gene expression databases

BgeeiENSMUSG00000064289.
ExpressionAtlasiP70347. baseline and differential.
GenevisibleiP70347. MM.

Interactioni

Subunit structurei

Homodimer. Found in a deubiquitination complex with TANK, USP10 and ZC3H12A; this complex inhibits genotoxic stress- or interleukin-1-beta-mediated NF-kappaB activation by promoting IKBKG or TRAF6 deubiquitination. Interacts with IKBKG; this interaction increases in response to DNA damage. Interacts with TRAF6; this interaction increases in response to DNA damage and recruits USP10 to the ubiquitinated TRAF6. Interacts with USP10; this interaction increases in response to DNA damage. Interacts with TBK1 and IKBKE. Interacts also with TRAF1, TRAF2, and TRAF3 by binding to their TRAF-C domains; the interaction with TRAF2 is disrupted by the phosphorylation of TANK by IKBKE. Interacts more strongly with TRAF1 and TRAF2 than TRAF3. Part of a ternary complex consisting of TANK, IKBKB and IKBKG (By similarity). Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1 (PubMed:12133833).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Tbk1Q9WUN26EBI-646116,EBI-764193
Traf1P3942812EBI-646125,EBI-520123
Traf2P394297EBI-646125,EBI-520016

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-33701N.
IntActiP70347. 4 interactors.
STRINGi10090.ENSMUSP00000077219.

Structurei

3D structure databases

ProteinModelPortaliP70347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 65Necessary for interaction with ZC3H12ABy similarityAdd BLAST31
Regioni105 – 224Necessary for interaction with TRAF6By similarityAdd BLAST120
Regioni166 – 205Interaction with TBK1 and IKBKEAdd BLAST40
Regioni205 – 224TRAF family member interactionAdd BLAST20

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili60 – 98Sequence analysisAdd BLAST39

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri417 – 443C2H2-typeAdd BLAST27

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGRF. Eukaryota.
ENOG410Y7D3. LUCA.
GeneTreeiENSGT00390000008712.
HOGENOMiHOG000231816.
HOVERGENiHBG019299.
InParanoidiP70347.
KOiK12650.
OMAiNVKFPPT.
OrthoDBiEOG091G0A8P.
PhylomeDBiP70347.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70347-1) [UniParc]FASTAAdd to basket
Also known as: Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF
60 70 80 90 100
RQACMDRDSA VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV
110 120 130 140 150
DSSRDNSYGY VPLLEDSDRR KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV
160 170 180 190 200
SSGKESAKGL NIPLHHERDN IEKTFWDLKE EFHRICLLAK AQKDHLSKLN
210 220 230 240 250
IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS CVTAVTPRGL
260 270 280 290 300
GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD
310 320 330 340 350
RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT
360 370 380 390 400
CLPAGDHNVF YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD
410 420 430 440
TDLVVPSDSD SELLKPLVCE FCQELFPPSI TSRGDFLRHL NTHFNGET
Length:448
Mass (Da):50,939
Last modified:February 1, 1997 - v1
Checksum:i313E704C881FB5F3
GO
Isoform 2 (identifier: P70347-2) [UniParc]FASTAAdd to basket
Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Show »
Length:414
Mass (Da):47,147
Checksum:iBE53BC1B0915BF21
GO
Isoform 3 (identifier: P70347-3) [UniParc]FASTAAdd to basket
Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     238-251: GMSCVTAVTPRGLG → ERRVCQLETTMCSM
     252-448: Missing.

Show »
Length:251
Mass (Da):29,255
Checksum:iDE48310FBE7188B9
GO
Isoform 4 (identifier: P70347-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-395: PFWKP → VTVLH
     396-448: Missing.

Show »
Length:395
Mass (Da):44,827
Checksum:i0457CDB69425C0A0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Missing in AAB02204 (PubMed:8608943).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0044441 – 34Missing in isoform 2. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_004445238 – 251GMSCV…PRGLG → ERRVCQLETTMCSM in isoform 3. CuratedAdd BLAST14
Alternative sequenceiVSP_004446252 – 448Missing in isoform 3. CuratedAdd BLAST197
Alternative sequenceiVSP_004447391 – 395PFWKP → VTVLH in isoform 4. Curated5
Alternative sequenceiVSP_004448396 – 448Missing in isoform 4. CuratedAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59864 mRNA. Translation: AAC52781.1.
U51907 mRNA. Translation: AAB02204.1.
CCDSiCCDS16061.1. [P70347-1]
CCDS50592.1. [P70347-2]
RefSeqiNP_001157544.1. NM_001164072.1.
NP_035659.1. NM_011529.2. [P70347-1]
XP_006499151.1. XM_006499088.3. [P70347-2]
XP_017172402.1. XM_017316913.1.
UniGeneiMm.244393.

Genome annotation databases

EnsembliENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
GeneIDi21353.
KEGGimmu:21353.
UCSCiuc008juw.2. mouse. [P70347-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59864 mRNA. Translation: AAC52781.1.
U51907 mRNA. Translation: AAB02204.1.
CCDSiCCDS16061.1. [P70347-1]
CCDS50592.1. [P70347-2]
RefSeqiNP_001157544.1. NM_001164072.1.
NP_035659.1. NM_011529.2. [P70347-1]
XP_006499151.1. XM_006499088.3. [P70347-2]
XP_017172402.1. XM_017316913.1.
UniGeneiMm.244393.

3D structure databases

ProteinModelPortaliP70347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-33701N.
IntActiP70347. 4 interactors.
STRINGi10090.ENSMUSP00000077219.

PTM databases

iPTMnetiP70347.
PhosphoSitePlusiP70347.

Proteomic databases

MaxQBiP70347.
PaxDbiP70347.
PRIDEiP70347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
GeneIDi21353.
KEGGimmu:21353.
UCSCiuc008juw.2. mouse. [P70347-1]

Organism-specific databases

CTDi10010.
MGIiMGI:107676. Tank.

Phylogenomic databases

eggNOGiENOG410IGRF. Eukaryota.
ENOG410Y7D3. LUCA.
GeneTreeiENSGT00390000008712.
HOGENOMiHOG000231816.
HOVERGENiHBG019299.
InParanoidiP70347.
KOiK12650.
OMAiNVKFPPT.
OrthoDBiEOG091G0A8P.
PhylomeDBiP70347.

Miscellaneous databases

ChiTaRSiTank. mouse.
PROiP70347.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064289.
ExpressionAtlasiP70347. baseline and differential.
GenevisibleiP70347. MM.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTANK_MOUSE
AccessioniPrimary (citable) accession number: P70347
Secondary accession number(s): Q61178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.