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Protein

TRAF family member-associated NF-kappa-B activator

Gene

Tank

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri417 – 44327C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  1. I-kappaB kinase/NF-kappaB signaling Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198521. TRAF6 mediated IRF7 activation.
REACT_225463. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.

Names & Taxonomyi

Protein namesi
Recommended name:
TRAF family member-associated NF-kappa-B activator
Alternative name(s):
TRAF-interacting protein
Short name:
I-TRAF
Gene namesi
Name:Tank
Synonyms:Itraf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:107676. Tank.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448TRAF family member-associated NF-kappa-B activatorPRO_0000072428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111PhosphoserineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei380 – 3801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70347.
PRIDEiP70347.

PTM databases

PhosphoSiteiP70347.

Expressioni

Tissue specificityi

Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Gene expression databases

BgeeiP70347.
ExpressionAtlasiP70347. baseline and differential.
GenevestigatoriP70347.

Interactioni

Subunit structurei

Homodimer. Interacts with TBK1 and IKBKE. Interacts also with TRAF1, TRAF2, and TRAF3 by binding to their TRAF-C domains; the interaction with TRAF2 is disrupted by the phosphorylation of TANK by IKBKE. Interacts more strongly with TRAF1 and TRAF2 than TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Tbk1Q9WUN26EBI-646116,EBI-764193
Traf1P3942812EBI-646125,EBI-520123
Traf2P394297EBI-646125,EBI-520016

Protein-protein interaction databases

DIPiDIP-33701N.
IntActiP70347. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP70347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 20540Interaction with TBK1 and IKBKEAdd
BLAST
Regioni205 – 22420TRAF family member interactionAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili60 – 9839Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri417 – 44327C2H2-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG44252.
GeneTreeiENSGT00390000008712.
HOGENOMiHOG000231816.
HOVERGENiHBG019299.
InParanoidiP70347.
KOiK12650.
OMAiFPNQDSD.
PhylomeDBiP70347.

Family and domain databases

InterProiIPR024581. TBD.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70347-1) [UniParc]FASTAAdd to basket

Also known as: Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF
60 70 80 90 100
RQACMDRDSA VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV
110 120 130 140 150
DSSRDNSYGY VPLLEDSDRR KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV
160 170 180 190 200
SSGKESAKGL NIPLHHERDN IEKTFWDLKE EFHRICLLAK AQKDHLSKLN
210 220 230 240 250
IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS CVTAVTPRGL
260 270 280 290 300
GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD
310 320 330 340 350
RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT
360 370 380 390 400
CLPAGDHNVF YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD
410 420 430 440
TDLVVPSDSD SELLKPLVCE FCQELFPPSI TSRGDFLRHL NTHFNGET
Length:448
Mass (Da):50,939
Last modified:February 1, 1997 - v1
Checksum:i313E704C881FB5F3
GO
Isoform 2 (identifier: P70347-2) [UniParc]FASTAAdd to basket

Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Show »
Length:414
Mass (Da):47,147
Checksum:iBE53BC1B0915BF21
GO
Isoform 3 (identifier: P70347-3) [UniParc]FASTAAdd to basket

Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     238-251: GMSCVTAVTPRGLG → ERRVCQLETTMCSM
     252-448: Missing.

Show »
Length:251
Mass (Da):29,255
Checksum:iDE48310FBE7188B9
GO
Isoform 4 (identifier: P70347-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-395: PFWKP → VTVLH
     396-448: Missing.

Show »
Length:395
Mass (Da):44,827
Checksum:i0457CDB69425C0A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681Missing in AAB02204 (PubMed:8608943).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3434Missing in isoform 2. 1 PublicationVSP_004444Add
BLAST
Alternative sequencei238 – 25114GMSCV…PRGLG → ERRVCQLETTMCSM in isoform 3. CuratedVSP_004445Add
BLAST
Alternative sequencei252 – 448197Missing in isoform 3. CuratedVSP_004446Add
BLAST
Alternative sequencei391 – 3955PFWKP → VTVLH in isoform 4. CuratedVSP_004447
Alternative sequencei396 – 44853Missing in isoform 4. CuratedVSP_004448Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59864 mRNA. Translation: AAC52781.1.
U51907 mRNA. Translation: AAB02204.1.
CCDSiCCDS16061.1. [P70347-1]
CCDS50592.1. [P70347-2]
RefSeqiNP_001157544.1. NM_001164072.1.
NP_035659.1. NM_011529.2. [P70347-1]
XP_006499151.1. XM_006499088.1. [P70347-2]
UniGeneiMm.244393.

Genome annotation databases

EnsembliENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
GeneIDi21353.
KEGGimmu:21353.
UCSCiuc008juw.2. mouse. [P70347-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59864 mRNA. Translation: AAC52781.1.
U51907 mRNA. Translation: AAB02204.1.
CCDSiCCDS16061.1. [P70347-1]
CCDS50592.1. [P70347-2]
RefSeqiNP_001157544.1. NM_001164072.1.
NP_035659.1. NM_011529.2. [P70347-1]
XP_006499151.1. XM_006499088.1. [P70347-2]
UniGeneiMm.244393.

3D structure databases

ProteinModelPortaliP70347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-33701N.
IntActiP70347. 4 interactions.

PTM databases

PhosphoSiteiP70347.

Proteomic databases

MaxQBiP70347.
PRIDEiP70347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
GeneIDi21353.
KEGGimmu:21353.
UCSCiuc008juw.2. mouse. [P70347-1]

Organism-specific databases

CTDi10010.
MGIiMGI:107676. Tank.

Phylogenomic databases

eggNOGiNOG44252.
GeneTreeiENSGT00390000008712.
HOGENOMiHOG000231816.
HOVERGENiHBG019299.
InParanoidiP70347.
KOiK12650.
OMAiFPNQDSD.
PhylomeDBiP70347.

Enzyme and pathway databases

ReactomeiREACT_198521. TRAF6 mediated IRF7 activation.
REACT_225463. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.

Miscellaneous databases

ChiTaRSiTank. mouse.
NextBioi300548.
PROiP70347.
SOURCEiSearch...

Gene expression databases

BgeeiP70347.
ExpressionAtlasiP70347. baseline and differential.
GenevestigatoriP70347.

Family and domain databases

InterProiIPR024581. TBD.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction."
    Rothe M., Xiong J., Shu H.-B., Williamson K., Goddard A., Goeddel D.V.
    Proc. Natl. Acad. Sci. U.S.A. 93:8241-8246(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation."
    Cheng G., Baltimore D.
    Genes Dev. 10:963-973(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/Kaplan.
    Tissue: T-cell lymphoma.
  3. "Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases."
    Chariot A., Leonardi A., Muller J., Bonif M., Brown K., Siebenlist U.
    J. Biol. Chem. 277:37029-37036(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IKBKG.

Entry informationi

Entry nameiTANK_MOUSE
AccessioniPrimary (citable) accession number: P70347
Secondary accession number(s): Q61178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: March 4, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.