Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P70347

- TANK_MOUSE

UniProt

P70347 - TANK_MOUSE

Protein

TRAF family member-associated NF-kappa-B activator

Gene

Tank

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri417 – 44327C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. I-kappaB kinase/NF-kappaB signaling Source: MGI

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198521. TRAF6 mediated IRF7 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TRAF family member-associated NF-kappa-B activator
    Alternative name(s):
    TRAF-interacting protein
    Short name:
    I-TRAF
    Gene namesi
    Name:Tank
    Synonyms:Itraf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:107676. Tank.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 448448TRAF family member-associated NF-kappa-B activatorPRO_0000072428Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei377 – 3771PhosphoserineBy similarity
    Modified residuei380 – 3801PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP70347.

    PTM databases

    PhosphoSiteiP70347.

    Expressioni

    Tissue specificityi

    Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

    Gene expression databases

    ArrayExpressiP70347.
    BgeeiP70347.
    GenevestigatoriP70347.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with TBK1 and IKBKE. Interacts also with TRAF1, TRAF2, and TRAF3 by binding to their TRAF-C domains; the interaction with TRAF2 is disrupted by the phosphorylation of TANK by IKBKE. Interacts more strongly with TRAF1 and TRAF2 than TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Tbk1Q9WUN26EBI-646116,EBI-764193
    Traf1P3942812EBI-646125,EBI-520123
    Traf2P394297EBI-646125,EBI-520016

    Protein-protein interaction databases

    DIPiDIP-33701N.
    IntActiP70347. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP70347.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 20540Interaction with TBK1 and IKBKEAdd
    BLAST
    Regioni205 – 22420TRAF family member interactionAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili60 – 9839Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri417 – 44327C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG44252.
    GeneTreeiENSGT00390000008712.
    HOGENOMiHOG000231816.
    HOVERGENiHBG019299.
    InParanoidiP70347.
    KOiK12650.
    OMAiLHSTPER.
    PhylomeDBiP70347.

    Family and domain databases

    InterProiIPR024581. TBD.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF12845. TBD. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P70347-1) [UniParc]FASTAAdd to Basket

    Also known as: Beta

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF    50
    RQACMDRDSA VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV 100
    DSSRDNSYGY VPLLEDSDRR KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV 150
    SSGKESAKGL NIPLHHERDN IEKTFWDLKE EFHRICLLAK AQKDHLSKLN 200
    IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS CVTAVTPRGL 250
    GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD 300
    RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT 350
    CLPAGDHNVF YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD 400
    TDLVVPSDSD SELLKPLVCE FCQELFPPSI TSRGDFLRHL NTHFNGET 448
    Length:448
    Mass (Da):50,939
    Last modified:February 1, 1997 - v1
    Checksum:i313E704C881FB5F3
    GO
    Isoform 2 (identifier: P70347-2) [UniParc]FASTAAdd to Basket

    Also known as: Alpha

    The sequence of this isoform differs from the canonical sequence as follows:
         1-34: Missing.

    Show »
    Length:414
    Mass (Da):47,147
    Checksum:iBE53BC1B0915BF21
    GO
    Isoform 3 (identifier: P70347-3) [UniParc]FASTAAdd to Basket

    Also known as: Gamma

    The sequence of this isoform differs from the canonical sequence as follows:
         238-251: GMSCVTAVTPRGLG → ERRVCQLETTMCSM
         252-448: Missing.

    Show »
    Length:251
    Mass (Da):29,255
    Checksum:iDE48310FBE7188B9
    GO
    Isoform 4 (identifier: P70347-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         391-395: PFWKP → VTVLH
         396-448: Missing.

    Show »
    Length:395
    Mass (Da):44,827
    Checksum:i0457CDB69425C0A0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681Missing in AAB02204. (PubMed:8608943)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3434Missing in isoform 2. 1 PublicationVSP_004444Add
    BLAST
    Alternative sequencei238 – 25114GMSCV…PRGLG → ERRVCQLETTMCSM in isoform 3. CuratedVSP_004445Add
    BLAST
    Alternative sequencei252 – 448197Missing in isoform 3. CuratedVSP_004446Add
    BLAST
    Alternative sequencei391 – 3955PFWKP → VTVLH in isoform 4. CuratedVSP_004447
    Alternative sequencei396 – 44853Missing in isoform 4. CuratedVSP_004448Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59864 mRNA. Translation: AAC52781.1.
    U51907 mRNA. Translation: AAB02204.1.
    CCDSiCCDS16061.1. [P70347-1]
    CCDS50592.1. [P70347-2]
    RefSeqiNP_001157544.1. NM_001164072.1.
    NP_035659.1. NM_011529.2. [P70347-1]
    XP_006499151.1. XM_006499088.1. [P70347-2]
    UniGeneiMm.244393.

    Genome annotation databases

    EnsembliENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
    ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
    GeneIDi21353.
    KEGGimmu:21353.
    UCSCiuc008juw.2. mouse. [P70347-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59864 mRNA. Translation: AAC52781.1 .
    U51907 mRNA. Translation: AAB02204.1 .
    CCDSi CCDS16061.1. [P70347-1 ]
    CCDS50592.1. [P70347-2 ]
    RefSeqi NP_001157544.1. NM_001164072.1.
    NP_035659.1. NM_011529.2. [P70347-1 ]
    XP_006499151.1. XM_006499088.1. [P70347-2 ]
    UniGenei Mm.244393.

    3D structure databases

    ProteinModelPortali P70347.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-33701N.
    IntActi P70347. 4 interactions.

    PTM databases

    PhosphoSitei P70347.

    Proteomic databases

    PRIDEi P70347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000078074 ; ENSMUSP00000077219 ; ENSMUSG00000064289 . [P70347-1 ]
    ENSMUST00000112501 ; ENSMUSP00000108120 ; ENSMUSG00000064289 . [P70347-2 ]
    GeneIDi 21353.
    KEGGi mmu:21353.
    UCSCi uc008juw.2. mouse. [P70347-1 ]

    Organism-specific databases

    CTDi 10010.
    MGIi MGI:107676. Tank.

    Phylogenomic databases

    eggNOGi NOG44252.
    GeneTreei ENSGT00390000008712.
    HOGENOMi HOG000231816.
    HOVERGENi HBG019299.
    InParanoidi P70347.
    KOi K12650.
    OMAi LHSTPER.
    PhylomeDBi P70347.

    Enzyme and pathway databases

    Reactomei REACT_198521. TRAF6 mediated IRF7 activation.

    Miscellaneous databases

    ChiTaRSi TANK. mouse.
    NextBioi 300548.
    PROi P70347.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70347.
    Bgeei P70347.
    Genevestigatori P70347.

    Family and domain databases

    InterProi IPR024581. TBD.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF12845. TBD. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction."
      Rothe M., Xiong J., Shu H.-B., Williamson K., Goddard A., Goeddel D.V.
      Proc. Natl. Acad. Sci. U.S.A. 93:8241-8246(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    2. "TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation."
      Cheng G., Baltimore D.
      Genes Dev. 10:963-973(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57BL/Kaplan.
      Tissue: T-cell lymphoma.
    3. "Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases."
      Chariot A., Leonardi A., Muller J., Bonif M., Brown K., Siebenlist U.
      J. Biol. Chem. 277:37029-37036(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IKBKG.

    Entry informationi

    Entry nameiTANK_MOUSE
    AccessioniPrimary (citable) accession number: P70347
    Secondary accession number(s): Q61178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3