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P70347 (TANK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TRAF family member-associated NF-kappa-B activator
Alternative name(s):
TRAF-interacting protein
Short name=I-TRAF
Gene names
Name:Tank
Synonyms:Itraf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.

Subunit structure

Homodimer. Interacts with TBK1 and IKBKE. Interacts also with TRAF1, TRAF2, and TRAF3 by binding to their TRAF-C domains; the interaction with TRAF2 is disrupted by the phosphorylation of TANK by IKBKE. Interacts more strongly with TRAF1 and TRAF2 than TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Ref.3

Subcellular location

Cytoplasm.

Tissue specificity

Heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processI-kappaB kinase/NF-kappaB signaling

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70347-1)

Also known as: Beta;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70347-2)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.
Isoform 3 (identifier: P70347-3)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     238-251: GMSCVTAVTPRGLG → ERRVCQLETTMCSM
     252-448: Missing.
Isoform 4 (identifier: P70347-4)

The sequence of this isoform differs from the canonical sequence as follows:
     391-395: PFWKP → VTVLH
     396-448: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448TRAF family member-associated NF-kappa-B activator
PRO_0000072428

Regions

Zinc finger417 – 44327C2H2-type
Region166 – 20540Interaction with TBK1 and IKBKE
Region205 – 22420TRAF family member interaction

Amino acid modifications

Modified residue3771Phosphoserine By similarity
Modified residue3801Phosphoserine By similarity

Natural variations

Alternative sequence1 – 3434Missing in isoform 2.
VSP_004444
Alternative sequence238 – 25114GMSCV…PRGLG → ERRVCQLETTMCSM in isoform 3.
VSP_004445
Alternative sequence252 – 448197Missing in isoform 3.
VSP_004446
Alternative sequence391 – 3955PFWKP → VTVLH in isoform 4.
VSP_004447
Alternative sequence396 – 44853Missing in isoform 4.
VSP_004448

Experimental info

Sequence conflict681Missing in AAB02204. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Beta) [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 313E704C881FB5F3

FASTA44850,939
        10         20         30         40         50         60 
MSLKRHSLRR NACHLETRAG IPTILYSDAT GQRGMDKNIG EQLNRAYEAF RQACMDRDSA 

        70         80         90        100        110        120 
VRELQQKQTE NYEQRIREQQ EQLSFQQNLI DRLKSQLLLV DSSRDNSYGY VPLLEDSDRR 

       130        140        150        160        170        180 
KNNLTLDEPH DKVKLGTLRD KQSKVRRQEV SSGKESAKGL NIPLHHERDN IEKTFWDLKE 

       190        200        210        220        230        240 
EFHRICLLAK AQKDHLSKLN IPDIATDTQC SVPIQCTDKT EKQEALFKPQ AKDDINRGMS 

       250        260        270        280        290        300 
CVTAVTPRGL GRDEEDTSFE SLSKFNVKFP PMDNDSIFLH STPEAPSILA PATPETVCQD 

       310        320        330        340        350        360 
RFNMEVRDNP GNFVKTEETL FEIQGIDPIT SAIQNLKTTD KTNPSNLRAT CLPAGDHNVF 

       370        380        390        400        410        420 
YVNTFPLQDP PDAPFPSLDS PGKAVRGPQQ PFWKPFLNQD TDLVVPSDSD SELLKPLVCE 

       430        440 
FCQELFPPSI TSRGDFLRHL NTHFNGET 

« Hide

Isoform 2 (Alpha) [UniParc].

Checksum: BE53BC1B0915BF21
Show »

FASTA41447,147
Isoform 3 (Gamma) [UniParc].

Checksum: DE48310FBE7188B9
Show »

FASTA25129,255
Isoform 4 [UniParc].

Checksum: 0457CDB69425C0A0
Show »

FASTA39544,827

References

[1]"I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction."
Rothe M., Xiong J., Shu H.-B., Williamson K., Goddard A., Goeddel D.V.
Proc. Natl. Acad. Sci. U.S.A. 93:8241-8246(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]"TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation."
Cheng G., Baltimore D.
Genes Dev. 10:963-973(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/Kaplan.
Tissue: T-cell lymphoma.
[3]"Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases."
Chariot A., Leonardi A., Muller J., Bonif M., Brown K., Siebenlist U.
J. Biol. Chem. 277:37029-37036(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IKBKG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59864 mRNA. Translation: AAC52781.1.
U51907 mRNA. Translation: AAB02204.1.
RefSeqNP_001157544.1. NM_001164072.1.
NP_035659.1. NM_011529.2.
XP_006499151.1. XM_006499088.1.
UniGeneMm.244393.

3D structure databases

ProteinModelPortalP70347.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-33701N.
IntActP70347. 4 interactions.

PTM databases

PhosphoSiteP70347.

Proteomic databases

PRIDEP70347.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000078074; ENSMUSP00000077219; ENSMUSG00000064289. [P70347-1]
ENSMUST00000112501; ENSMUSP00000108120; ENSMUSG00000064289. [P70347-2]
GeneID21353.
KEGGmmu:21353.
UCSCuc008juw.2. mouse. [P70347-1]

Organism-specific databases

CTD10010.
MGIMGI:107676. Tank.

Phylogenomic databases

eggNOGNOG44252.
GeneTreeENSGT00390000008712.
HOGENOMHOG000231816.
HOVERGENHBG019299.
InParanoidP70347.
KOK12650.
OMALHSTPER.
PhylomeDBP70347.

Gene expression databases

ArrayExpressP70347.
BgeeP70347.
GenevestigatorP70347.

Family and domain databases

InterProIPR024581. TBD.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF12845. TBD. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTANK. mouse.
NextBio300548.
PROP70347.
SOURCESearch...

Entry information

Entry nameTANK_MOUSE
AccessionPrimary (citable) accession number: P70347
Secondary accession number(s): Q61178
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot