P70345 (B2CL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bcl-2-like protein 2
Short name=Bcl2-L-2

Alternative name(s):
Apoptosis regulator Bcl-W
c98

Gene names

Name:	Bcl2l2
Synonyms:	Bclw, Kiaa0271

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Ref.1 Ref.3 Ref.7
Subunit structure	Interacts with BOP By similarity.
Subcellular location	Mitochondrion membrane; Peripheral membrane protein By similarity. Note: Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane By similarity.
Tissue specificity	Expressed in almost all myeloid cell lines and in a wide range of tissues, with highest levels in brain, colon, and salivary gland.
Developmental stage	Expressed in both mitotic and postmitotic Sertoli cells. Ref.7
Induction	By Igf1. Ref.3
Domain	The BH4 motif seems to be involved in the anti-apoptotic function. The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2 By similarity.
Disruption phenotype	Mice are sterile due to arrest in spermatogenesis associated with a gradual loss of germ and Sertoli cells from the testis. Ref.2
Sequence similarities	Belongs to the Bcl-2 family.
Sequence caution	The sequence BAD32203.1 differs from that shown. Reason: Frameshift at position 191.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Sertoli cell proliferation Inferred from genetic interaction Ref.7. Source: MGI apoptotic process Inferred from direct assay PubMed 12115603. Source: MGI negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay PubMed 11980919. Source: MGI mitochondrial membrane Inferred from direct assay PubMed 11980919. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P70345-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P70345-2)

The sequence of this isoform differs from the canonical sequence as follows:
     145-193: AEFTALYGDG...VTVGAFFASK → VRSSQLLLSASLYKVGLHGKIGPLMGGWGCAGKG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Bcl-2-like protein 2		PRO_0000143067
Regions
	Motif	9 – 29	21	BH4
	Motif	85 – 104	20	BH1
	Motif	136 – 151	16	BH2
Natural variations
	Alternative sequence	145 – 193	49	AEFTA…FFASK → VRSSQLLLSASLYKVGLHGK IGPLMGGWGCAGKG in isoform 2.		VSP_014780
Experimental info
	Sequence conflict	16	1	D → Y in AAO13177. Ref.3
	Sequence conflict	21	1	K → Q in AAO13177. Ref.3
	Sequence conflict	53 – 54	2	FE → LQ in AAO13177. Ref.3
	Sequence conflict	63	1	D → H in AAO13177. Ref.3
	Sequence conflict	114	1	E → H in AAO13177. Ref.3
	Sequence conflict	136	1	D → Y in AAO13177. Ref.3
	Sequence conflict	192	1	S → Y in AAO13177. Ref.3
Isoform 2:
	Sequence conflict	177	1	K → R in BAB28740. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 36CA185F5945DFB4	FASTA	193	20,790
	10 20 30 40 50 60 MATPASTPDT RALVADFVGY KLRQKGYVCG AGPGEGPAAD PLHQAMRAAG DEFETRFRRT 70 80 90 100 110 120 FSDLAAQLHV TPGSAQQRFT QVSDELFQGG PNWGRLVAFF VFGAALCAES VNKEMEPLVG 130 140 150 160 170 180 QVQDWMVAYL ETRLADWIHS SGGWAEFTAL YGDGALEEAR RLREGNWASV RTVLTGAVAL 190 GALVTVGAFF ASK
	Isoform 2 [UniParc]. Checksum: E2C3F3F79528E9D7	FASTA	178	19,119
	10 20 30 40 50 60 MATPASTPDT RALVADFVGY KLRQKGYVCG AGPGEGPAAD PLHQAMRAAG DEFETRFRRT 70 80 90 100 110 120 FSDLAAQLHV TPGSAQQRFT QVSDELFQGG PNWGRLVAFF VFGAALCAES VNKEMEPLVG 130 140 150 160 170 QVQDWMVAYL ETRLADWIHS SGGWVRSSQL LLSASLYKVG LHGKIGPLMG GWGCAGKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bcl-w, a novel member of the Bcl-2 family, promotes cell survival." Gibson L., Holmgreen S.P., Huang D.C., Bernard O., Copeland N.G., Jenkins N.A., Sutherland G.R., Baker E., Adams J.M., Cory S. Oncogene 13:665-675(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Testicular degeneration in Bclw-deficient mice." Ross A.J., Waymire K.G., Moss J.E., Parlow A.F., Skinner M.K., Russell L.D., Macgregor G.R. Nat. Genet. 18:251-256(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DISRUPTION PHENOTYPE. Strain: C57BL/10J.
[3]	"Mouse keratinocytes express c98, a novel gene homologous to bcl-2, that is stimulated by insulin-like growth factor 1 and prevents dexamethasone-induced apoptosis." Su H.-Y., Cheng W.T.K., Chen S.-C., Lin C.-T., Lien Y.-Y., Liu H.-J., Gilmour R.S. Biochim. Biophys. Acta 1676:127-137(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION. Strain: C57BL/6. Tissue: Skin.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Lung and Testis.
[5]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreatic islet.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Retina.
[7]	"BCLW mediates survival of postmitotic Sertoli cells by regulating BAX activity." Ross A.J., Amy S.P., Mahar P.L., Lindsten T., Knudson C.M., Thompson C.B., Korsmeyer S.J., MacGregor G.R. Dev. Biol. 239:295-308(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59746 mRNA. Translation: AAB09056.1. AF030769 mRNA. Translation: AAB86430.1. AY170344 mRNA. Translation: AAO13177.2. AK004680 mRNA. Translation: BAB23468.1. AK013244 mRNA. Translation: BAB28740.1. AK015644 mRNA. Translation: BAB29912.1. AK172925 mRNA. Translation: BAD32203.1. Frameshift. BC040369 mRNA. Translation: AAH40369.1.
IPI	IPI00108184. IPI00229366.
RefSeq	NP_031563.1. NM_007537.1.
UniGene	Mm.397611. Mm.6967.
3D structure databases
ProteinModelPortal	P70345.
SMR	P70345. Positions 1-183.
ModBase	Search...
PTM databases
PhosphoSite	P70345.
Proteomic databases
PaxDb	P70345.
PRIDE	P70345.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022806; ENSMUSP00000022806; ENSMUSG00000089682. ENSMUST00000133397; ENSMUSP00000116385; ENSMUSG00000089682.
GeneID	12050.
KEGG	mmu:12050.
UCSC	uc007txe.1. mouse. uc007txf.1. mouse.
Organism-specific databases
CTD	599.
MGI	MGI:108052. Bcl2l2.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG238057.
GeneTree	ENSGT00530000062935.
HOGENOM	HOG000056452.
InParanoid	P70345.
KO	K02163.
OMA	VYLETRL.
OrthoDB	EOG4RBQKR.
Gene expression databases
ArrayExpress	P70345.
Bgee	P70345.
CleanEx	MM_BCL2L2.
Genevestigator	P70345.
GermOnline	ENSMUSG00000022194. Mus musculus.
Family and domain databases
InterPro	IPR013280. Apop_reg_BclW. IPR002475. Bcl2-like. IPR020717. Bcl2_BH1_motif_CS. IPR020726. Bcl2_BH2_motif_CS. IPR003093. Bcl2_BH4. IPR020731. Bcl2_BH4_motif_CS. IPR026298. Blc2_fam. [Graphical view]
PANTHER	PTHR11256. PTHR11256. 1 hit. PTHR11256:SF13. PTHR11256:SF13. 1 hit.
Pfam	PF00452. Bcl-2. 1 hit. PF02180. BH4. 1 hit. [Graphical view]
PRINTS	PR01865. APOPREGBCLW. PR01862. BCL2FAMILY.
SMART	SM00265. BH4. 1 hit. [Graphical view]
PROSITE	PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01260. BH4_1. 1 hit. PS50063. BH4_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BCL2L2. mouse.
NextBio	280343.
SOURCE	Search...

Entry information

Entry name

B2CL2_MOUSE

Accession

Primary (citable) accession number: P70345
Secondary accession number(s): Q545Q4 , Q6A093, Q8CFR2, Q8CGL4, Q9CYW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program