Reviewed, UniProtKB/Swiss-Prot P70343 (CASP4_MOUSE)

Names and origin

Protein names

Recommended name:
    Caspase-4
      Short name=CASP-4
    EC=3.4.22.64
Alternative name(s):
    Caspase-11
      Short name=CASP-11
    ICH-3 protease
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-4 subunit p10
    2- Recommended name:
            Caspase-4 subunit p20

Gene names

Name:	Casp4
Synonyms:	Casp11, Caspl, Ich3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
Catalytic activity	Strict requirement for Asp at the P1 position and has a preferred cleavage sequence of (Ile/Leu/Val/Phe)-Gly-His-Asp-|-.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit.
Tissue specificity	Mostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
Induction	Activity increased 30-fold by endotoxins (LPS).
Post-translational modification	The two subunits are derived from the precursor sequence by a autocatalytic mechanism.
Sequence similarities	Belongs to the peptidase C14A family. Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Zymogen
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptosis Inferred from genetic interaction. Source: MGI
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 80	80	Potential		PRO_0000004600
	Chain	81 – 266	186	Caspase-4 subunit p20		PRO_0000004601
	Propeptide	267 – 285	19	Potential		PRO_0000004602
	Chain	286 – 373	88	Caspase-4 subunit p10		PRO_0000004603
Regions
	Domain	1 – 91	91	CARD
Sites
	Active site	206	1	By similarity
	Active site	254	1
Amino acid modifications
	Modified residue	83	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	254	1	C → G: No cell death.
	Sequence conflict	152	1	K → N in CAA73531. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P70343-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 574B0EC01804E0B4	FASTA	373	42,756
	10 20 30 40 50 60 MAENKHPDKP LKVLEQLGKE VLTEYLEKLV QSNVLKLKEE DKQKFNNAER SDKRWVFVDA 70 80 90 100 110 120 MKKKHSKVGE MLLQTFFSVD PGSHHGEANL EMEEPEESLN TLKLCSPEEF TRLCREKTQE 130 140 150 160 170 180 IYPIKEANGR TRKALIICNT EFKHLSLRYG AKFDIIGMKG LLEDLGYDVV VKEELTAEGM 190 200 210 220 230 240 ESEMKDFAAL SEHQTSDSTF LVLMSHGTLH GICGTMHSEK TPDVLQYDTI YQIFNNCHCP 250 260 270 280 290 300 GLRDKPKVII VQACRGGNSG EMWIRESSKP QLCRGVDLPR NMEADAVKLS HVEKDFIAFY 310 320 330 340 350 360 STTPHHLSYR DKTGGSYFIT RLISCFRKHA CSCHLFDIFL KVQQSFEKAS IHSQMPTIDR 370 ATLTRYFYLF PGN

References

[1]	"Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE." Wang S., Miura M., Jung Y.-K., Zhu H., Gagliardini V., Shi L., Greenberg A.H., Yuan J. J. Biol. Chem. 271:20580-20587(1996) [PubMed: 8702803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[2]	"Characterization of seven murine caspase family members." van de Craen M., Vandenabeele P., Declercq W., van den Brande I., van Loo G., Molemans F., Schotte P., van Criekinge W., Beyaert R., Fiers W. FEBS Lett. 403:61-69(1997) [PubMed: 9038361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/An.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59463 mRNA. Translation: AAB09469.1. Y13089 mRNA. Translation: CAA73531.1.
IPI	IPI00108178.
UniGene	Mm.1569
3D structure databases
HSSP	HSSP built from PDB template 1ICE based on UniProtKB P29466.
ModBase	Search...
Protein family/group databases
MEROPS	C14.012.
PTM databases
PhosphoSite	P70343.
Genome annotation databases
Ensembl	ENSMUSG00000033538. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107700. Casp4.
Phylogenomic databases
HOGENOM	P70343.
HOVERGEN	P70343.
Enzyme and pathway databases
BRENDA	3.4.22.57. 244. 3.4.22.64. 244.
Gene expression databases
ArrayExpress	P70343.
Bgee	P70343.
CleanEx	MM_CASP4.
GermOnline	ENSMUSG00000033538. Mus musculus.
Family and domain databases
InterPro	IPR001315. CARD. IPR017350. Caspase_IL-1_beta. IPR011029. DEATH-like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view]
PIRSF	PIRSF038001. Caspase_ICE. 1 hit.
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CASP4_MOUSE

Accession

Primary (citable) accession number: P70343
Secondary accession number(s): O08735

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)