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Protein

MyoD family inhibitor

Gene

Mdfi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.4 Publications

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • activation of JUN kinase activity Source: UniProtKB
  • dorsal/ventral axis specification Source: UniProtKB
  • embryonic skeletal system morphogenesis Source: MGI
  • negative regulation of DNA binding Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • regulation of Wnt signaling pathway Source: UniProtKB
  • trophoblast giant cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
MyoD family inhibitor
Alternative name(s):
Myogenic repressor I-mf
Gene namesi
Name:Mdfi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107687. Mdfi.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251MyoD family inhibitorPRO_0000096325Add
BLAST

Proteomic databases

PRIDEiP70331.

Expressioni

Tissue specificityi

In the embryo, highly expressed in the sclerotome. Also expressed in the notochord, neural tube, limb buds, heart, branchial arches and head mesenchyme. In the adult, highly expressed in skeletal muscle. Expressed at lower levels in most other tissues.1 Publication

Gene expression databases

BgeeiP70331.
CleanExiMM_MDFI.
ExpressionAtlasiP70331. baseline and differential.
GenevestigatoriP70331.

Interactioni

Subunit structurei

The C-terminus interacts with AXIN1 and LEF1.1 Publication

Protein-protein interaction databases

BioGridi201368. 2 interactions.
MINTiMINT-1519234.

Structurei

3D structure databases

ProteinModelPortaliP70331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MDFI family.Curated

Phylogenomic databases

eggNOGiNOG43212.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
InParanoidiP70331.
OMAiQAQEDCC.
PhylomeDBiP70331.
TreeFamiTF332113.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform I-mfB (identifier: P70331-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQVSGQCPS RCDAPHGVPS AALDPAQTMS LLPGLEVARS THPVEASSEE
60 70 80 90 100
GFPEEAAPSM PHDSGLRAQQ ALNSIDLDVP TEAVTCQPQG NPQGCTPLLP
110 120 130 140 150
NGSSHDHLSE PGSAGHAGNG ALGGSKAHRK LQTHPSLGSQ AGRKSRGSAR
160 170 180 190 200
SASQVPLQAQ EGKAPAVRIH RQTASPTCCL RNAQLSGTAL RSLRLESQGH
210 220 230 240 250
RELNNKTLSQ SNNKKPGVAA HAAIIPALTR PKQNCHDPSL LPGTHGVGKE

F
Length:251
Mass (Da):26,013
Last modified:February 1, 1997 - v1
Checksum:iE77D693D935F1B83
GO
Isoform I-mfA (identifier: P70331-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     162-251: GKAPAVRIHR...PGTHGVGKEF → DCCVHCILSC...MECCGLCFSS

Show »
Length:246
Mass (Da):25,270
Checksum:i138A37A2B4A10463
GO
Isoform I-mfC (identifier: P70331-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-163: SLGSQAGRKSRGSARSASQVPLQAQEGK → PQPPNRSAFRRVAWSPPRVTRNTASRAW
     164-251: Missing.

Show »
Length:163
Mass (Da):17,012
Checksum:iDD58E66BF9793D27
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei136 – 16328SLGSQ…AQEGK → PQPPNRSAFRRVAWSPPRVT RNTASRAW in isoform I-mfC. 1 PublicationVSP_004055Add
BLAST
Alternative sequencei162 – 25190GKAPA…VGKEF → DCCVHCILSCLFCEFLTLCN ILLDCATCGSCSSEDSCLCC CCCGSGECADCDLPCDLDCG IVDACCESADCLEICMECCG LCFSS in isoform I-mfA. 2 PublicationsVSP_004056Add
BLAST
Alternative sequencei164 – 25188Missing in isoform I-mfC. 1 PublicationVSP_004057Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57820 mRNA. Translation: AAC52791.1.
U57821 mRNA. Translation: AAC52792.1.
U57822 mRNA. Translation: AAC52793.1.
BC006018 mRNA. Translation: AAH06018.1.
BC010259 mRNA. Translation: AAH10259.1.
BC083058 mRNA. Translation: AAH83058.1.
BC085233 mRNA. Translation: AAH85233.1.
CCDSiCCDS50134.1. [P70331-2]
RefSeqiNP_001103443.1. NM_001109973.2. [P70331-2]
NP_001263319.1. NM_001276390.1. [P70331-2]
NP_034913.2. NM_010783.3. [P70331-2]
XP_006523806.1. XM_006523743.2. [P70331-1]
XP_006523807.1. XM_006523744.2. [P70331-1]
XP_006523808.1. XM_006523745.2. [P70331-1]
UniGeneiMm.329100.

Genome annotation databases

EnsembliENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717. [P70331-2]
ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717. [P70331-2]
GeneIDi17240.
KEGGimmu:17240.
UCSCiuc008cwh.2. mouse. [P70331-2]
uc008cwj.2. mouse. [P70331-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57820 mRNA. Translation: AAC52791.1.
U57821 mRNA. Translation: AAC52792.1.
U57822 mRNA. Translation: AAC52793.1.
BC006018 mRNA. Translation: AAH06018.1.
BC010259 mRNA. Translation: AAH10259.1.
BC083058 mRNA. Translation: AAH83058.1.
BC085233 mRNA. Translation: AAH85233.1.
CCDSiCCDS50134.1. [P70331-2]
RefSeqiNP_001103443.1. NM_001109973.2. [P70331-2]
NP_001263319.1. NM_001276390.1. [P70331-2]
NP_034913.2. NM_010783.3. [P70331-2]
XP_006523806.1. XM_006523743.2. [P70331-1]
XP_006523807.1. XM_006523744.2. [P70331-1]
XP_006523808.1. XM_006523745.2. [P70331-1]
UniGeneiMm.329100.

3D structure databases

ProteinModelPortaliP70331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201368. 2 interactions.
MINTiMINT-1519234.

Proteomic databases

PRIDEiP70331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717. [P70331-2]
ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717. [P70331-2]
GeneIDi17240.
KEGGimmu:17240.
UCSCiuc008cwh.2. mouse. [P70331-2]
uc008cwj.2. mouse. [P70331-1]

Organism-specific databases

CTDi4188.
MGIiMGI:107687. Mdfi.

Phylogenomic databases

eggNOGiNOG43212.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
InParanoidiP70331.
OMAiQAQEDCC.
PhylomeDBiP70331.
TreeFamiTF332113.

Miscellaneous databases

NextBioi291686.
PROiP70331.
SOURCEiSearch...

Gene expression databases

BgeeiP70331.
CleanExiMM_MDFI.
ExpressionAtlasiP70331. baseline and differential.
GenevestigatoriP70331.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "I-mf, a novel myogenic repressor, interacts with members of the MyoD family."
    Chen C.-M.A., Kraut N., Groudine M., Weintraub H.
    Cell 86:731-741(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I-MFA; I-MFB AND I-MFC), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: NIH Swiss.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I-MFA).
    Strain: 129 and FVB/N.
    Tissue: Mammary gland.
  3. "Requirement of the mouse I-mfa gene for placental development and skeletal patterning."
    Kraut N., Snider L., Chen C.-M.A., Tapscott S.J., Groudine M.
    EMBO J. 17:6276-6288(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "I-mfa domain proteins interact with Axin and affect its regulation of the Wnt and c-Jun N-terminal kinase signaling pathways."
    Kusano S., Raab-Traub N.
    Mol. Cell. Biol. 22:6393-6405(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AXIN1 AND LEF1.

Entry informationi

Entry nameiMDFI_MOUSE
AccessioniPrimary (citable) accession number: P70331
Secondary accession number(s): P70330
, P70332, Q5XK64, Q99JM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 1, 1997
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.