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P70331

- MDFI_MOUSE

UniProt

P70331 - MDFI_MOUSE

Protein

MyoD family inhibitor

Gene

Mdfi

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. activation of JUN kinase activity Source: UniProtKB
    2. dorsal/ventral axis specification Source: UniProtKB
    3. embryonic skeletal system morphogenesis Source: MGI
    4. negative regulation of DNA binding Source: UniProtKB
    5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    6. negative regulation of Wnt signaling pathway Source: UniProtKB
    7. regulation of Wnt signaling pathway Source: UniProtKB
    8. trophoblast giant cell differentiation Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MyoD family inhibitor
    Alternative name(s):
    Myogenic repressor I-mf
    Gene namesi
    Name:Mdfi
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:107687. Mdfi.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251MyoD family inhibitorPRO_0000096325Add
    BLAST

    Proteomic databases

    PRIDEiP70331.

    Expressioni

    Tissue specificityi

    In the embryo, highly expressed in the sclerotome. Also expressed in the notochord, neural tube, limb buds, heart, branchial arches and head mesenchyme. In the adult, highly expressed in skeletal muscle. Expressed at lower levels in most other tissues.1 Publication

    Gene expression databases

    ArrayExpressiP70331.
    BgeeiP70331.
    CleanExiMM_MDFI.
    GenevestigatoriP70331.

    Interactioni

    Subunit structurei

    The C-terminus interacts with AXIN1 and LEF1.1 Publication

    Protein-protein interaction databases

    BioGridi201368. 2 interactions.
    MINTiMINT-1519234.

    Structurei

    3D structure databases

    ProteinModelPortaliP70331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MDFI family.Curated

    Phylogenomic databases

    eggNOGiNOG43212.
    GeneTreeiENSGT00390000010600.
    HOGENOMiHOG000001577.
    InParanoidiQ5XK64.
    OMAiQAQEDCC.
    PhylomeDBiP70331.
    TreeFamiTF332113.

    Family and domain databases

    InterProiIPR026134. MDFI/MDFIC.
    [Graphical view]
    PANTHERiPTHR15304. PTHR15304. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform I-mfB (identifier: P70331-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQVSGQCPS RCDAPHGVPS AALDPAQTMS LLPGLEVARS THPVEASSEE    50
    GFPEEAAPSM PHDSGLRAQQ ALNSIDLDVP TEAVTCQPQG NPQGCTPLLP 100
    NGSSHDHLSE PGSAGHAGNG ALGGSKAHRK LQTHPSLGSQ AGRKSRGSAR 150
    SASQVPLQAQ EGKAPAVRIH RQTASPTCCL RNAQLSGTAL RSLRLESQGH 200
    RELNNKTLSQ SNNKKPGVAA HAAIIPALTR PKQNCHDPSL LPGTHGVGKE 250
    F 251
    Length:251
    Mass (Da):26,013
    Last modified:February 1, 1997 - v1
    Checksum:iE77D693D935F1B83
    GO
    Isoform I-mfA (identifier: P70331-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         162-251: GKAPAVRIHR...PGTHGVGKEF → DCCVHCILSC...MECCGLCFSS

    Show »
    Length:246
    Mass (Da):25,270
    Checksum:i138A37A2B4A10463
    GO
    Isoform I-mfC (identifier: P70331-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         136-163: SLGSQAGRKSRGSARSASQVPLQAQEGK → PQPPNRSAFRRVAWSPPRVTRNTASRAW
         164-251: Missing.

    Show »
    Length:163
    Mass (Da):17,012
    Checksum:iDD58E66BF9793D27
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei136 – 16328SLGSQ…AQEGK → PQPPNRSAFRRVAWSPPRVT RNTASRAW in isoform I-mfC. 1 PublicationVSP_004055Add
    BLAST
    Alternative sequencei162 – 25190GKAPA…VGKEF → DCCVHCILSCLFCEFLTLCN ILLDCATCGSCSSEDSCLCC CCCGSGECADCDLPCDLDCG IVDACCESADCLEICMECCG LCFSS in isoform I-mfA. 2 PublicationsVSP_004056Add
    BLAST
    Alternative sequencei164 – 25188Missing in isoform I-mfC. 1 PublicationVSP_004057Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57820 mRNA. Translation: AAC52791.1.
    U57821 mRNA. Translation: AAC52792.1.
    U57822 mRNA. Translation: AAC52793.1.
    BC006018 mRNA. Translation: AAH06018.1.
    BC010259 mRNA. Translation: AAH10259.1.
    BC083058 mRNA. Translation: AAH83058.1.
    BC085233 mRNA. Translation: AAH85233.1.
    CCDSiCCDS50134.1. [P70331-2]
    RefSeqiNP_001103443.1. NM_001109973.2. [P70331-2]
    NP_001263319.1. NM_001276390.1. [P70331-2]
    NP_034913.2. NM_010783.3. [P70331-2]
    XP_006523806.1. XM_006523743.1. [P70331-1]
    XP_006523807.1. XM_006523744.1. [P70331-1]
    XP_006523808.1. XM_006523745.1. [P70331-1]
    UniGeneiMm.329100.

    Genome annotation databases

    EnsembliENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717. [P70331-2]
    ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717. [P70331-2]
    GeneIDi17240.
    KEGGimmu:17240.
    UCSCiuc008cwh.2. mouse. [P70331-2]
    uc008cwj.2. mouse. [P70331-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57820 mRNA. Translation: AAC52791.1 .
    U57821 mRNA. Translation: AAC52792.1 .
    U57822 mRNA. Translation: AAC52793.1 .
    BC006018 mRNA. Translation: AAH06018.1 .
    BC010259 mRNA. Translation: AAH10259.1 .
    BC083058 mRNA. Translation: AAH83058.1 .
    BC085233 mRNA. Translation: AAH85233.1 .
    CCDSi CCDS50134.1. [P70331-2 ]
    RefSeqi NP_001103443.1. NM_001109973.2. [P70331-2 ]
    NP_001263319.1. NM_001276390.1. [P70331-2 ]
    NP_034913.2. NM_010783.3. [P70331-2 ]
    XP_006523806.1. XM_006523743.1. [P70331-1 ]
    XP_006523807.1. XM_006523744.1. [P70331-1 ]
    XP_006523808.1. XM_006523745.1. [P70331-1 ]
    UniGenei Mm.329100.

    3D structure databases

    ProteinModelPortali P70331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201368. 2 interactions.
    MINTi MINT-1519234.

    Proteomic databases

    PRIDEi P70331.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035375 ; ENSMUSP00000037888 ; ENSMUSG00000032717 . [P70331-2 ]
    ENSMUST00000066368 ; ENSMUSP00000069915 ; ENSMUSG00000032717 . [P70331-2 ]
    GeneIDi 17240.
    KEGGi mmu:17240.
    UCSCi uc008cwh.2. mouse. [P70331-2 ]
    uc008cwj.2. mouse. [P70331-1 ]

    Organism-specific databases

    CTDi 4188.
    MGIi MGI:107687. Mdfi.

    Phylogenomic databases

    eggNOGi NOG43212.
    GeneTreei ENSGT00390000010600.
    HOGENOMi HOG000001577.
    InParanoidi Q5XK64.
    OMAi QAQEDCC.
    PhylomeDBi P70331.
    TreeFami TF332113.

    Miscellaneous databases

    NextBioi 291686.
    PROi P70331.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70331.
    Bgeei P70331.
    CleanExi MM_MDFI.
    Genevestigatori P70331.

    Family and domain databases

    InterProi IPR026134. MDFI/MDFIC.
    [Graphical view ]
    PANTHERi PTHR15304. PTHR15304. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "I-mf, a novel myogenic repressor, interacts with members of the MyoD family."
      Chen C.-M.A., Kraut N., Groudine M., Weintraub H.
      Cell 86:731-741(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I-MFA; I-MFB AND I-MFC), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: NIH Swiss.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I-MFA).
      Strain: 129 and FVB/N.
      Tissue: Mammary gland.
    3. "Requirement of the mouse I-mfa gene for placental development and skeletal patterning."
      Kraut N., Snider L., Chen C.-M.A., Tapscott S.J., Groudine M.
      EMBO J. 17:6276-6288(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "I-mfa domain proteins interact with Axin and affect its regulation of the Wnt and c-Jun N-terminal kinase signaling pathways."
      Kusano S., Raab-Traub N.
      Mol. Cell. Biol. 22:6393-6405(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AXIN1 AND LEF1.

    Entry informationi

    Entry nameiMDFI_MOUSE
    AccessioniPrimary (citable) accession number: P70331
    Secondary accession number(s): P70330
    , P70332, Q5XK64, Q99JM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3