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P70331 (MDFI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MyoD family inhibitor
Alternative name(s):
Myogenic repressor I-mf
Gene names
Name:Mdfi
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Ref.1 Ref.3 Ref.4 Ref.5

Subunit structure

The C-terminus interacts with AXIN1 and LEF1. Ref.5

Subcellular location

Nucleus. Cytoplasm Ref.1 Ref.4.

Tissue specificity

In the embryo, highly expressed in the sclerotome. Also expressed in the notochord, neural tube, limb buds, heart, branchial arches and head mesenchyme. In the adult, highly expressed in skeletal muscle. Expressed at lower levels in most other tissues. Ref.1

Sequence similarities

Belongs to the MDFI family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform I-mfB (identifier: P70331-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform I-mfA (identifier: P70331-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-251: GKAPAVRIHR...PGTHGVGKEF → DCCVHCILSC...MECCGLCFSS
Isoform I-mfC (identifier: P70331-3)

The sequence of this isoform differs from the canonical sequence as follows:
     136-163: SLGSQAGRKSRGSARSASQVPLQAQEGK → PQPPNRSAFRRVAWSPPRVTRNTASRAW
     164-251: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251MyoD family inhibitor
PRO_0000096325

Natural variations

Alternative sequence136 – 16328SLGSQ…AQEGK → PQPPNRSAFRRVAWSPPRVT RNTASRAW in isoform I-mfC.
VSP_004055
Alternative sequence162 – 25190GKAPA…VGKEF → DCCVHCILSCLFCEFLTLCN ILLDCATCGSCSSEDSCLCC CCCGSGECADCDLPCDLDCG IVDACCESADCLEICMECCG LCFSS in isoform I-mfA.
VSP_004056
Alternative sequence164 – 25188Missing in isoform I-mfC.
VSP_004057

Sequences

Sequence LengthMass (Da)Tools
Isoform I-mfB [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E77D693D935F1B83

FASTA25126,013
        10         20         30         40         50         60 
MSQVSGQCPS RCDAPHGVPS AALDPAQTMS LLPGLEVARS THPVEASSEE GFPEEAAPSM 

        70         80         90        100        110        120 
PHDSGLRAQQ ALNSIDLDVP TEAVTCQPQG NPQGCTPLLP NGSSHDHLSE PGSAGHAGNG 

       130        140        150        160        170        180 
ALGGSKAHRK LQTHPSLGSQ AGRKSRGSAR SASQVPLQAQ EGKAPAVRIH RQTASPTCCL 

       190        200        210        220        230        240 
RNAQLSGTAL RSLRLESQGH RELNNKTLSQ SNNKKPGVAA HAAIIPALTR PKQNCHDPSL 

       250 
LPGTHGVGKE F 

« Hide

Isoform I-mfA [UniParc].

Checksum: 138A37A2B4A10463
Show »

FASTA24625,270
Isoform I-mfC [UniParc].

Checksum: DD58E66BF9793D27
Show »

FASTA16317,012

References

« Hide 'large scale' references
[1]"I-mf, a novel myogenic repressor, interacts with members of the MyoD family."
Chen C.-M.A., Kraut N., Groudine M., Weintraub H.
Cell 86:731-741(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I-MFA; I-MFB AND I-MFC), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: NIH Swiss.
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I-MFA).
Strain: 129 and FVB/N.
Tissue: Mammary gland.
[3]"Requirement of the mouse I-mfa gene for placental development and skeletal patterning."
Kraut N., Snider L., Chen C.-M.A., Tapscott S.J., Groudine M.
EMBO J. 17:6276-6288(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Inhibition of Tcf3 binding by I-mfa domain proteins."
Snider L., Thirlwell H., Miller J.R., Moon R.T., Groudine M., Tapscott S.J.
Mol. Cell. Biol. 21:1866-1873(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"I-mfa domain proteins interact with Axin and affect its regulation of the Wnt and c-Jun N-terminal kinase signaling pathways."
Kusano S., Raab-Traub N.
Mol. Cell. Biol. 22:6393-6405(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AXIN1 AND LEF1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57820 mRNA. Translation: AAC52791.1.
U57821 mRNA. Translation: AAC52792.1.
U57822 mRNA. Translation: AAC52793.1.
BC006018 mRNA. Translation: AAH06018.1.
BC010259 mRNA. Translation: AAH10259.1.
BC083058 mRNA. Translation: AAH83058.1.
BC085233 mRNA. Translation: AAH85233.1.
CCDSCCDS50134.1. [P70331-2]
RefSeqNP_001103443.1. NM_001109973.2. [P70331-2]
NP_001263319.1. NM_001276390.1. [P70331-2]
NP_034913.2. NM_010783.3. [P70331-2]
XP_006523806.1. XM_006523743.1. [P70331-1]
XP_006523807.1. XM_006523744.1. [P70331-1]
XP_006523808.1. XM_006523745.1. [P70331-1]
UniGeneMm.329100.

3D structure databases

ProteinModelPortalP70331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201368. 2 interactions.
MINTMINT-1519234.

Proteomic databases

PRIDEP70331.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717. [P70331-2]
ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717. [P70331-2]
GeneID17240.
KEGGmmu:17240.
UCSCuc008cwh.2. mouse. [P70331-2]
uc008cwj.2. mouse. [P70331-1]

Organism-specific databases

CTD4188.
MGIMGI:107687. Mdfi.

Phylogenomic databases

eggNOGNOG43212.
GeneTreeENSGT00390000010600.
HOGENOMHOG000001577.
InParanoidQ5XK64.
OMAQAQEDCC.
PhylomeDBP70331.
TreeFamTF332113.

Gene expression databases

ArrayExpressP70331.
BgeeP70331.
CleanExMM_MDFI.
GenevestigatorP70331.

Family and domain databases

InterProIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERPTHR15304. PTHR15304. 1 hit.
ProtoNetSearch...

Other

NextBio291686.
PROP70331.
SOURCESearch...

Entry information

Entry nameMDFI_MOUSE
AccessionPrimary (citable) accession number: P70331
Secondary accession number(s): P70330 expand/collapse secondary AC list , P70332, Q5XK64, Q99JM9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot