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Protein

T-box transcription factor TBX5

Gene

Tbx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi58 – 238181T-boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  3. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  4. sequence-specific DNA binding Source: UniProtKB
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  6. transcription factor binding Source: MGI

GO - Biological processi

  1. apoptotic nuclear changes Source: MGI
  2. atrial septum development Source: MGI
  3. atrial septum morphogenesis Source: MGI
  4. atrioventricular valve morphogenesis Source: MGI
  5. bundle of His development Source: MGI
  6. cardiac left ventricle formation Source: BHF-UCL
  7. cardiac muscle cell differentiation Source: BHF-UCL
  8. cell-cell signaling Source: UniProtKB
  9. embryonic forelimb morphogenesis Source: UniProtKB
  10. embryonic limb morphogenesis Source: UniProtKB
  11. endocardial cushion development Source: MGI
  12. forelimb morphogenesis Source: MGI
  13. heart development Source: UniProtKB
  14. lung development Source: MGI
  15. morphogenesis of an epithelium Source: MGI
  16. negative regulation of cardiac muscle cell proliferation Source: UniProtKB
  17. negative regulation of cell migration Source: UniProtKB
  18. negative regulation of cell proliferation Source: UniProtKB
  19. pattern specification process Source: MGI
  20. pericardium development Source: UniProtKB
  21. positive regulation of cardiac muscle cell proliferation Source: MGI
  22. positive regulation of cardioblast differentiation Source: UniProtKB
  23. positive regulation of secondary heart field cardioblast proliferation Source: MGI
  24. positive regulation of transcription, DNA-templated Source: UniProtKB
  25. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  26. regulation of execution phase of apoptosis Source: UniProtKB
  27. regulation of transcription, DNA-templated Source: MGI
  28. ventricular cardiac muscle tissue development Source: MGI
  29. ventricular septum development Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
T-box transcription factor TBX5
Short name:
T-box protein 5
Gene namesi
Name:Tbx5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:102541. Tbx5.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518T-box transcription factor TBX5PRO_0000184436Add
BLAST

Proteomic databases

PaxDbiP70326.
PRIDEiP70326.

PTM databases

PhosphoSiteiP70326.

Expressioni

Developmental stagei

First expressed at day 7.5, exclusively in the allantois. At day 8.5, expressed only in the sinous venosus of the developing heart. At day 9.5, expression observed in the forelimb bud, optic vesicle and sinous venosus. By day 12.5, expression is limited to the retina, the body wall and the mesenchyme of the lung, of the mandible and of that surrounding the trachea.1 Publication

Gene expression databases

BgeeiP70326.
CleanExiMM_TBX5.
ExpressionAtlasiP70326. baseline and differential.
GenevestigatoriP70326.

Interactioni

Subunit structurei

Monomer. Interacts with GATA4.By similarity

Protein-protein interaction databases

BioGridi203990. 3 interactions.
DIPiDIP-48887N.
IntActiP70326. 1 interaction.
MINTiMINT-4137187.

Structurei

3D structure databases

ProteinModelPortaliP70326.
SMRiP70326. Positions 52-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG320009.
HOGENOMiHOG000286001.
HOVERGENiHBG007310.
InParanoidiP70326.
KOiK10179.
OMAiSKDLPCD.
OrthoDBiEOG7GN2PM.
PhylomeDBiP70326.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTDEGFGL ARTPLEPDSK DRSCDSKPES ALGAPSKSPS SPQAAFTQQG
60 70 80 90 100
MEGIKVFLHE RELWLKFHEV GTEMIITKAG RRMFPSYKVK VTGLNPKTKY
110 120 130 140 150
ILLMDIVPAD DHRYKFADNK WSVTGKAEPA MPGRLYVHPD SPATGAHWMR
160 170 180 190 200
QLVSFQKLKL TNNHLDPFGH IILNSMHKYQ PRLHIVKADE NNGFGSKNTA
210 220 230 240 250
FCTHVFPETA FIAVTSYQNH KITQLKIENN PFAKGFRGSD DLELHRMSRM
260 270 280 290 300
QSKEYPVVPR STVRHKVTSN HSPFSSETRA LSTSSNLGSQ YQCENGVSGP
310 320 330 340 350
SQDLLPPPNP YPLAQEHSQI YHCTKRKDEE CSSTEHPYKK PYMETSPSEE
360 370 380 390 400
DTFYRSGYPQ QQGLSTSYRT ESAQRQACMY ASSAPPSEPV PSLEDISCNT
410 420 430 440 450
WPSMPSYSSC TVTTVQPMDR LPYQHFSAHF TSGPLVPRLA GMANHGSPQL
460 470 480 490 500
GEGMFQHQTS VAHQPVVRQC GPQTGLQSPG GLQPPEFLYT HGVPRTLSPH
510
QYHSVHGVGM VPEWSENS
Length:518
Mass (Da):57,832
Last modified:December 1, 2000 - v2
Checksum:i4990A802EB56624B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 714HEVG → RSGV in AAC53109 (PubMed:8878690).Curated
Sequence conflicti81 – 811R → G in AAC53109 (PubMed:8878690).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140427 mRNA. Translation: AAF00112.1.
U57330 mRNA. Translation: AAC53109.1.
CCDSiCCDS19615.1.
PIRiS72231.
RefSeqiNP_035667.1. NM_011537.3.
XP_006530343.1. XM_006530280.1.
XP_006530344.1. XM_006530281.2.
XP_006530345.1. XM_006530282.2.
XP_006530346.1. XM_006530283.1.
UniGeneiMm.103636.

Genome annotation databases

EnsembliENSMUST00000018407; ENSMUSP00000018407; ENSMUSG00000018263.
GeneIDi21388.
KEGGimmu:21388.
UCSCiuc008zgz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140427 mRNA. Translation: AAF00112.1.
U57330 mRNA. Translation: AAC53109.1.
CCDSiCCDS19615.1.
PIRiS72231.
RefSeqiNP_035667.1. NM_011537.3.
XP_006530343.1. XM_006530280.1.
XP_006530344.1. XM_006530281.2.
XP_006530345.1. XM_006530282.2.
XP_006530346.1. XM_006530283.1.
UniGeneiMm.103636.

3D structure databases

ProteinModelPortaliP70326.
SMRiP70326. Positions 52-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203990. 3 interactions.
DIPiDIP-48887N.
IntActiP70326. 1 interaction.
MINTiMINT-4137187.

PTM databases

PhosphoSiteiP70326.

Proteomic databases

PaxDbiP70326.
PRIDEiP70326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018407; ENSMUSP00000018407; ENSMUSG00000018263.
GeneIDi21388.
KEGGimmu:21388.
UCSCiuc008zgz.2. mouse.

Organism-specific databases

CTDi6910.
MGIiMGI:102541. Tbx5.

Phylogenomic databases

eggNOGiNOG320009.
HOGENOMiHOG000286001.
HOVERGENiHBG007310.
InParanoidiP70326.
KOiK10179.
OMAiSKDLPCD.
OrthoDBiEOG7GN2PM.
PhylomeDBiP70326.
TreeFamiTF106341.

Enzyme and pathway databases

ReactomeiREACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Miscellaneous databases

NextBioi300636.
PROiP70326.
SOURCEiSearch...

Gene expression databases

BgeeiP70326.
CleanExiMM_TBX5.
ExpressionAtlasiP70326. baseline and differential.
GenevestigatoriP70326.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome."
    Bruneau B.G., Logan M., Davis N., Levi T., Tabin C.J., Seidman J.G., Seidman C.E.
    Dev. Biol. 211:100-108(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Limb.
  2. "Evolution of mouse T-box genes by tandem duplication and cluster dispersion."
    Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I., Bollag R.J., Papaioannou V.E., Silver L.M.
    Genetics 144:249-254(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-246.
    Tissue: Embryo.
  3. Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiTBX5_MOUSE
AccessioniPrimary (citable) accession number: P70326
Secondary accession number(s): Q9R003
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: April 1, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.