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P70324

- TBX3_MOUSE

UniProt

P70324 - TBX3_MOUSE

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Protein

T-box transcription factor TBX3

Gene

Tbx3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi107 – 220114T-box; first partPROSITE-ProRule annotationAdd
BLAST
DNA bindingi241 – 30565T-box; second partPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II activating transcription factor binding Source: BHF-UCL
  2. RNA polymerase II transcription factor binding Source: BHF-UCL
  3. sequence-specific DNA binding Source: UniProtKB
  4. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. anterior/posterior axis specification, embryo Source: UniProtKB
  2. atrioventricular bundle cell differentiation Source: MGI
  3. blood vessel development Source: MGI
  4. branching involved in mammary gland duct morphogenesis Source: MGI
  5. cardiac muscle cell differentiation Source: MGI
  6. cardiac muscle cell fate commitment Source: MGI
  7. cell aging Source: MGI
  8. cellular senescence Source: UniProtKB
  9. embryonic digit morphogenesis Source: UniProtKB
  10. embryonic forelimb morphogenesis Source: UniProtKB
  11. female genitalia development Source: UniProtKB
  12. follicle-stimulating hormone secretion Source: UniProtKB
  13. forelimb morphogenesis Source: MGI
  14. heart looping Source: MGI
  15. heart morphogenesis Source: MGI
  16. in utero embryonic development Source: MGI
  17. limbic system development Source: MGI
  18. limb morphogenesis Source: MGI
  19. luteinizing hormone secretion Source: UniProtKB
  20. male genitalia development Source: UniProtKB
  21. mammary gland development Source: MGI
  22. mammary placode formation Source: MGI
  23. mesoderm morphogenesis Source: UniProtKB
  24. negative regulation of apoptotic process Source: UniProtKB
  25. negative regulation of epithelial cell differentiation Source: MGI
  26. negative regulation of myoblast differentiation Source: UniProtKB
  27. negative regulation of transcription, DNA-templated Source: UniProtKB
  28. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  29. organ morphogenesis Source: MGI
  30. outflow tract morphogenesis Source: MGI
  31. palate development Source: MGI
  32. positive regulation of cell cycle Source: UniProtKB
  33. positive regulation of cell proliferation Source: UniProtKB
  34. positive regulation of stem cell proliferation Source: MGI
  35. positive regulation of transcription, DNA-templated Source: MGI
  36. regulation of cell proliferation Source: MGI
  37. sinoatrial node cell development Source: MGI
  38. skeletal system development Source: UniProtKB
  39. specification of organ position Source: MGI
  40. stem cell maintenance Source: MGI
  41. transcription, DNA-templated Source: UniProtKB-KW
  42. ventricular septum morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
T-box transcription factor TBX3
Short name:
T-box protein 3
Gene namesi
Name:Tbx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:98495. Tbx3.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741T-box transcription factor TBX3PRO_0000184429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei432 – 4321PhosphoserineBy similarity
Modified residuei438 – 4381PhosphoserineBy similarity
Modified residuei705 – 7051Phosphoserine1 Publication
Modified residuei736 – 7361PhosphoserineBy similarity
Modified residuei738 – 7381PhosphoserineBy similarity
Modified residuei740 – 7401PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70324.
PaxDbiP70324.
PRIDEiP70324.

PTM databases

PhosphoSiteiP70324.

Expressioni

Tissue specificityi

In adults, highest levels in lung. Also found in brain, heart, kidney, liver and ovary.

Developmental stagei

First expressed in the blastocyst at day 3.5. At day 7.5, expressed in the extraembryonic endoderm and in the mesoderm of the chorion and amnion. At day 9.5, in the facial region, forelimb, pharyngeal epithelium, mesenchyme of the pharyngeal arches and the lateral body wall and, at day 12.5, in the trigeminal ganglia, developing central nervous system and in the mammary buds.1 Publication

Gene expression databases

BgeeiP70324.
CleanExiMM_TBX3.
ExpressionAtlasiP70324. baseline and differential.
GenevestigatoriP70324.

Interactioni

Subunit structurei

Interacts with PML.By similarity

Protein-protein interaction databases

BioGridi203988. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP70324.
SMRiP70324. Positions 100-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG240293.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000038046.
HOVERGENiHBG000412.
InParanoidiP70324.
KOiK10177.
OMAiPSQFAMG.
OrthoDBiEOG7W9RV1.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR022582. TBX.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
PF12598. TBX. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P70324-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG
60 70 80 90 100
AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEDVE
110 120 130 140 150
DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI
160 170 180 190 200
LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK
210 220 230 240 250
VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP
260 270 280 290 300
RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA
310 320 330 340 350
KGFRDTGNGR REKRKQLTLQ SMRVFEERHK KETSDESSSE QAAFNCFAQA
360 370 380 390 400
SSPAVSIVGT SNLKDLCPSE AESDAEAESK EEHGPEACDA AKISTTTAEE
410 420 430 440 450
PGRDKGSPAT RAQLFPAEPS RARDTARLDK ASPDSRHSPA TISSSTRVPG
460 470 480 490 500
ADERRSPGRE GPVATKVDEA RAIPAKDAFA PLSVQTDATA HLAQGPLPGL
510 520 530 540 550
GFAPGLAGQQ FFNGHPLFLH PGQFAMGGAF SSMAAGMGPL LATVSGASTG
560 570 580 590 600
VSGLESTAMA SAAAAQGLSG ASAATLPFHL QQHVLASQGL AMSPFGSLFP
610 620 630 640 650
YPYTYMAAAA AASTAAASSS VHRHPFLNLN SMRPRLRYSP YSIPVPVPDS
660 670 680 690 700
SSLLATALPS MASAAGPLDG KAAALAASPA SVAVDSGSEL NSRSSTLSSG
710 720 730 740
SVSLSPKLCS EKEAATSELQ SIQRLVSGLE AKPDRSCSGS P
Length:741
Mass (Da):79,160
Last modified:July 27, 2011 - v3
Checksum:iB502FE817EFF124D
GO
Isoform 2 (identifier: P70324-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-240: Missing.

Show »
Length:721
Mass (Da):76,949
Checksum:iF086EA71843D4D2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti154 – 1552DI → T in AAC53107. (PubMed:8878690)Curated
Sequence conflicti184 – 1841M → R in AAC53107. (PubMed:8878690)Curated
Sequence conflicti306 – 3094TGNG → IGHC in AAC53107. (PubMed:8878690)Curated
Sequence conflicti569 – 5691S → Y in BAC34999. (PubMed:16141072)Curated
Sequence conflicti717 – 7171S → N in BAC34999. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei221 – 24020Missing in isoform 2. 2 PublicationsVSP_013377Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK052453 mRNA. Translation: BAC34999.1.
AC140675 Genomic DNA. No translation available.
U57328 mRNA. Translation: AAC53107.1.
U15567 mRNA. Translation: AAC52698.1.
CCDSiCCDS19613.1. [P70324-1]
CCDS19614.1. [P70324-2]
RefSeqiNP_035665.2. NM_011535.3. [P70324-1]
XP_006530339.1. XM_006530276.1. [P70324-1]
UniGeneiMm.219139.

Genome annotation databases

EnsembliENSMUST00000018748; ENSMUSP00000018748; ENSMUSG00000018604. [P70324-1]
ENSMUST00000079719; ENSMUSP00000078657; ENSMUSG00000018604. [P70324-2]
ENSMUST00000121021; ENSMUSP00000112519; ENSMUSG00000018604. [P70324-2]
GeneIDi21386.
KEGGimmu:21386.
UCSCiuc008zgu.1. mouse. [P70324-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK052453 mRNA. Translation: BAC34999.1 .
AC140675 Genomic DNA. No translation available.
U57328 mRNA. Translation: AAC53107.1 .
U15567 mRNA. Translation: AAC52698.1 .
CCDSi CCDS19613.1. [P70324-1 ]
CCDS19614.1. [P70324-2 ]
RefSeqi NP_035665.2. NM_011535.3. [P70324-1 ]
XP_006530339.1. XM_006530276.1. [P70324-1 ]
UniGenei Mm.219139.

3D structure databases

ProteinModelPortali P70324.
SMRi P70324. Positions 100-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203988. 3 interactions.

PTM databases

PhosphoSitei P70324.

Proteomic databases

MaxQBi P70324.
PaxDbi P70324.
PRIDEi P70324.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018748 ; ENSMUSP00000018748 ; ENSMUSG00000018604 . [P70324-1 ]
ENSMUST00000079719 ; ENSMUSP00000078657 ; ENSMUSG00000018604 . [P70324-2 ]
ENSMUST00000121021 ; ENSMUSP00000112519 ; ENSMUSG00000018604 . [P70324-2 ]
GeneIDi 21386.
KEGGi mmu:21386.
UCSCi uc008zgu.1. mouse. [P70324-1 ]

Organism-specific databases

CTDi 6926.
MGIi MGI:98495. Tbx3.

Phylogenomic databases

eggNOGi NOG240293.
GeneTreei ENSGT00760000118897.
HOGENOMi HOG000038046.
HOVERGENi HBG000412.
InParanoidi P70324.
KOi K10177.
OMAi PSQFAMG.
OrthoDBi EOG7W9RV1.
TreeFami TF106341.

Miscellaneous databases

NextBioi 300624.
PROi P70324.
SOURCEi Search...

Gene expression databases

Bgeei P70324.
CleanExi MM_TBX3.
ExpressionAtlasi P70324. baseline and differential.
Genevestigatori P70324.

Family and domain databases

Gene3Di 2.60.40.820. 1 hit.
InterProi IPR008967. p53-like_TF_DNA-bd.
IPR022582. TBX.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view ]
PANTHERi PTHR11267. PTHR11267. 1 hit.
Pfami PF00907. T-box. 1 hit.
PF12598. TBX. 1 hit.
[Graphical view ]
PRINTSi PR00937. TBOX.
SMARTi SM00425. TBOX. 1 hit.
[Graphical view ]
SUPFAMi SSF49417. SSF49417. 1 hit.
PROSITEi PS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Evolution of mouse T-box genes by tandem duplication and cluster dispersion."
    Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I., Bollag R.J., Papaioannou V.E., Silver L.M.
    Genetics 144:249-254(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 112-313 (ISOFORM 2).
    Tissue: Embryo.
  4. "An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus."
    Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., Silver L.M.
    Nat. Genet. 7:383-389(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-286 (ISOFORM 2).
    Tissue: Embryo.
  5. Cited for: DEVELOPMENTAL STAGE.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-705, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTBX3_MOUSE
AccessioniPrimary (citable) accession number: P70324
Secondary accession number(s): E9QM97, Q60708, Q8BWH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3