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Protein

T-box transcription factor TBX3

Gene

Tbx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi107 – 220114T-box; first partPROSITE-ProRule annotationAdd
BLAST
DNA bindingi241 – 30565T-box; second partPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II activating transcription factor binding Source: BHF-UCL
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
  4. RNA polymerase II transcription factor binding Source: BHF-UCL
  5. sequence-specific DNA binding Source: UniProtKB
  6. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. anterior/posterior axis specification, embryo Source: UniProtKB
  2. atrioventricular bundle cell differentiation Source: MGI
  3. blood vessel development Source: MGI
  4. branching involved in mammary gland duct morphogenesis Source: MGI
  5. cardiac muscle cell differentiation Source: MGI
  6. cardiac muscle cell fate commitment Source: MGI
  7. cell aging Source: MGI
  8. cellular senescence Source: UniProtKB
  9. embryonic digit morphogenesis Source: UniProtKB
  10. embryonic forelimb morphogenesis Source: UniProtKB
  11. embryonic heart tube development Source: MGI
  12. embryonic hindlimb morphogenesis Source: MGI
  13. female genitalia development Source: UniProtKB
  14. follicle-stimulating hormone secretion Source: UniProtKB
  15. forelimb morphogenesis Source: MGI
  16. heart looping Source: MGI
  17. heart morphogenesis Source: MGI
  18. in utero embryonic development Source: MGI
  19. limbic system development Source: MGI
  20. limb morphogenesis Source: MGI
  21. luteinizing hormone secretion Source: UniProtKB
  22. male genitalia development Source: UniProtKB
  23. mammary gland development Source: MGI
  24. mammary placode formation Source: MGI
  25. mesoderm morphogenesis Source: UniProtKB
  26. negative regulation of apoptotic process Source: UniProtKB
  27. negative regulation of epithelial cell differentiation Source: MGI
  28. negative regulation of myoblast differentiation Source: UniProtKB
  29. negative regulation of transcription, DNA-templated Source: UniProtKB
  30. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  31. organ morphogenesis Source: MGI
  32. outflow tract morphogenesis Source: MGI
  33. palate development Source: MGI
  34. positive regulation of cell cycle Source: UniProtKB
  35. positive regulation of cell proliferation Source: UniProtKB
  36. positive regulation of stem cell proliferation Source: MGI
  37. positive regulation of transcription, DNA-templated Source: MGI
  38. regulation of cell proliferation Source: MGI
  39. sinoatrial node cell development Source: MGI
  40. skeletal system development Source: UniProtKB
  41. specification of organ position Source: MGI
  42. stem cell maintenance Source: MGI
  43. ventricular septum morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
T-box transcription factor TBX3
Short name:
T-box protein 3
Gene namesi
Name:Tbx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:98495. Tbx3.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741T-box transcription factor TBX3PRO_0000184429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei432 – 4321PhosphoserineBy similarity
Modified residuei438 – 4381PhosphoserineBy similarity
Modified residuei705 – 7051Phosphoserine1 Publication
Modified residuei736 – 7361PhosphoserineBy similarity
Modified residuei738 – 7381PhosphoserineBy similarity
Modified residuei740 – 7401PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70324.
PaxDbiP70324.
PRIDEiP70324.

PTM databases

PhosphoSiteiP70324.

Expressioni

Tissue specificityi

In adults, highest levels in lung. Also found in brain, heart, kidney, liver and ovary.

Developmental stagei

First expressed in the blastocyst at day 3.5. At day 7.5, expressed in the extraembryonic endoderm and in the mesoderm of the chorion and amnion. At day 9.5, in the facial region, forelimb, pharyngeal epithelium, mesenchyme of the pharyngeal arches and the lateral body wall and, at day 12.5, in the trigeminal ganglia, developing central nervous system and in the mammary buds.1 Publication

Gene expression databases

BgeeiP70324.
CleanExiMM_TBX3.
ExpressionAtlasiP70324. baseline and differential.
GenevestigatoriP70324.

Interactioni

Subunit structurei

Interacts with PML.By similarity

Protein-protein interaction databases

BioGridi203988. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP70324.
SMRiP70324. Positions 100-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG240293.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000038046.
HOVERGENiHBG000412.
InParanoidiP70324.
KOiK10177.
OMAiPSQFAMG.
OrthoDBiEOG7W9RV1.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR022582. TBX.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
PF12598. TBX. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70324-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG
60 70 80 90 100
AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEDVE
110 120 130 140 150
DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI
160 170 180 190 200
LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK
210 220 230 240 250
VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP
260 270 280 290 300
RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA
310 320 330 340 350
KGFRDTGNGR REKRKQLTLQ SMRVFEERHK KETSDESSSE QAAFNCFAQA
360 370 380 390 400
SSPAVSIVGT SNLKDLCPSE AESDAEAESK EEHGPEACDA AKISTTTAEE
410 420 430 440 450
PGRDKGSPAT RAQLFPAEPS RARDTARLDK ASPDSRHSPA TISSSTRVPG
460 470 480 490 500
ADERRSPGRE GPVATKVDEA RAIPAKDAFA PLSVQTDATA HLAQGPLPGL
510 520 530 540 550
GFAPGLAGQQ FFNGHPLFLH PGQFAMGGAF SSMAAGMGPL LATVSGASTG
560 570 580 590 600
VSGLESTAMA SAAAAQGLSG ASAATLPFHL QQHVLASQGL AMSPFGSLFP
610 620 630 640 650
YPYTYMAAAA AASTAAASSS VHRHPFLNLN SMRPRLRYSP YSIPVPVPDS
660 670 680 690 700
SSLLATALPS MASAAGPLDG KAAALAASPA SVAVDSGSEL NSRSSTLSSG
710 720 730 740
SVSLSPKLCS EKEAATSELQ SIQRLVSGLE AKPDRSCSGS P
Length:741
Mass (Da):79,160
Last modified:July 26, 2011 - v3
Checksum:iB502FE817EFF124D
GO
Isoform 2 (identifier: P70324-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-240: Missing.

Show »
Length:721
Mass (Da):76,949
Checksum:iF086EA71843D4D2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti154 – 1552DI → T in AAC53107 (PubMed:8878690).Curated
Sequence conflicti184 – 1841M → R in AAC53107 (PubMed:8878690).Curated
Sequence conflicti306 – 3094TGNG → IGHC in AAC53107 (PubMed:8878690).Curated
Sequence conflicti569 – 5691S → Y in BAC34999 (PubMed:16141072).Curated
Sequence conflicti717 – 7171S → N in BAC34999 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei221 – 24020Missing in isoform 2. 2 PublicationsVSP_013377Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052453 mRNA. Translation: BAC34999.1.
AC140675 Genomic DNA. No translation available.
U57328 mRNA. Translation: AAC53107.1.
U15567 mRNA. Translation: AAC52698.1.
CCDSiCCDS19613.1. [P70324-1]
CCDS19614.1. [P70324-2]
RefSeqiNP_035665.2. NM_011535.3. [P70324-1]
XP_006530339.1. XM_006530276.2. [P70324-1]
UniGeneiMm.219139.

Genome annotation databases

EnsembliENSMUST00000018748; ENSMUSP00000018748; ENSMUSG00000018604. [P70324-1]
ENSMUST00000079719; ENSMUSP00000078657; ENSMUSG00000018604. [P70324-2]
ENSMUST00000121021; ENSMUSP00000112519; ENSMUSG00000018604. [P70324-2]
GeneIDi21386.
KEGGimmu:21386.
UCSCiuc008zgu.1. mouse. [P70324-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052453 mRNA. Translation: BAC34999.1.
AC140675 Genomic DNA. No translation available.
U57328 mRNA. Translation: AAC53107.1.
U15567 mRNA. Translation: AAC52698.1.
CCDSiCCDS19613.1. [P70324-1]
CCDS19614.1. [P70324-2]
RefSeqiNP_035665.2. NM_011535.3. [P70324-1]
XP_006530339.1. XM_006530276.2. [P70324-1]
UniGeneiMm.219139.

3D structure databases

ProteinModelPortaliP70324.
SMRiP70324. Positions 100-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203988. 3 interactions.

PTM databases

PhosphoSiteiP70324.

Proteomic databases

MaxQBiP70324.
PaxDbiP70324.
PRIDEiP70324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018748; ENSMUSP00000018748; ENSMUSG00000018604. [P70324-1]
ENSMUST00000079719; ENSMUSP00000078657; ENSMUSG00000018604. [P70324-2]
ENSMUST00000121021; ENSMUSP00000112519; ENSMUSG00000018604. [P70324-2]
GeneIDi21386.
KEGGimmu:21386.
UCSCiuc008zgu.1. mouse. [P70324-1]

Organism-specific databases

CTDi6926.
MGIiMGI:98495. Tbx3.

Phylogenomic databases

eggNOGiNOG240293.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000038046.
HOVERGENiHBG000412.
InParanoidiP70324.
KOiK10177.
OMAiPSQFAMG.
OrthoDBiEOG7W9RV1.
TreeFamiTF106341.

Miscellaneous databases

NextBioi300624.
PROiP70324.
SOURCEiSearch...

Gene expression databases

BgeeiP70324.
CleanExiMM_TBX3.
ExpressionAtlasiP70324. baseline and differential.
GenevestigatoriP70324.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR022582. TBX.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
PF12598. TBX. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Evolution of mouse T-box genes by tandem duplication and cluster dispersion."
    Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I., Bollag R.J., Papaioannou V.E., Silver L.M.
    Genetics 144:249-254(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 112-313 (ISOFORM 2).
    Tissue: Embryo.
  4. "An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus."
    Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., Silver L.M.
    Nat. Genet. 7:383-389(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-286 (ISOFORM 2).
    Tissue: Embryo.
  5. Cited for: DEVELOPMENTAL STAGE.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-705, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTBX3_MOUSE
AccessioniPrimary (citable) accession number: P70324
Secondary accession number(s): E9QM97, Q60708, Q8BWH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: July 26, 2011
Last modified: March 31, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.