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P70324

- TBX3_MOUSE

UniProt

P70324 - TBX3_MOUSE

Protein

T-box transcription factor TBX3

Gene

Tbx3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi107 – 220114T-box; first partPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi241 – 30565T-box; second partPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II activating transcription factor binding Source: BHF-UCL
    2. RNA polymerase II transcription factor binding Source: BHF-UCL
    3. sequence-specific DNA binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. anterior/posterior axis specification, embryo Source: UniProtKB
    2. atrioventricular bundle cell differentiation Source: MGI
    3. blood vessel development Source: MGI
    4. branching involved in mammary gland duct morphogenesis Source: MGI
    5. cardiac muscle cell differentiation Source: MGI
    6. cardiac muscle cell fate commitment Source: MGI
    7. cell aging Source: MGI
    8. cellular senescence Source: UniProtKB
    9. embryonic digit morphogenesis Source: UniProtKB
    10. embryonic forelimb morphogenesis Source: UniProtKB
    11. female genitalia development Source: UniProtKB
    12. follicle-stimulating hormone secretion Source: UniProtKB
    13. forelimb morphogenesis Source: MGI
    14. heart looping Source: MGI
    15. heart morphogenesis Source: MGI
    16. in utero embryonic development Source: MGI
    17. limbic system development Source: MGI
    18. limb morphogenesis Source: MGI
    19. luteinizing hormone secretion Source: UniProtKB
    20. male genitalia development Source: UniProtKB
    21. mammary gland development Source: MGI
    22. mammary placode formation Source: MGI
    23. mesoderm morphogenesis Source: UniProtKB
    24. negative regulation of apoptotic process Source: UniProtKB
    25. negative regulation of epithelial cell differentiation Source: MGI
    26. negative regulation of myoblast differentiation Source: UniProtKB
    27. negative regulation of transcription, DNA-templated Source: UniProtKB
    28. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    29. organ morphogenesis Source: MGI
    30. outflow tract morphogenesis Source: MGI
    31. palate development Source: MGI
    32. positive regulation of cell cycle Source: UniProtKB
    33. positive regulation of cell proliferation Source: UniProtKB
    34. positive regulation of stem cell proliferation Source: MGI
    35. positive regulation of transcription, DNA-templated Source: MGI
    36. regulation of cell proliferation Source: MGI
    37. sinoatrial node cell development Source: MGI
    38. skeletal system development Source: UniProtKB
    39. specification of organ position Source: MGI
    40. stem cell maintenance Source: MGI
    41. transcription, DNA-templated Source: UniProtKB-KW
    42. ventricular septum morphogenesis Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-box transcription factor TBX3
    Short name:
    T-box protein 3
    Gene namesi
    Name:Tbx3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:98495. Tbx3.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 741741T-box transcription factor TBX3PRO_0000184429Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei369 – 3691PhosphoserineBy similarity
    Modified residuei432 – 4321PhosphoserineBy similarity
    Modified residuei438 – 4381PhosphoserineBy similarity
    Modified residuei705 – 7051Phosphoserine1 Publication
    Modified residuei736 – 7361PhosphoserineBy similarity
    Modified residuei738 – 7381PhosphoserineBy similarity
    Modified residuei740 – 7401PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP70324.
    PaxDbiP70324.
    PRIDEiP70324.

    PTM databases

    PhosphoSiteiP70324.

    Expressioni

    Tissue specificityi

    In adults, highest levels in lung. Also found in brain, heart, kidney, liver and ovary.

    Developmental stagei

    First expressed in the blastocyst at day 3.5. At day 7.5, expressed in the extraembryonic endoderm and in the mesoderm of the chorion and amnion. At day 9.5, in the facial region, forelimb, pharyngeal epithelium, mesenchyme of the pharyngeal arches and the lateral body wall and, at day 12.5, in the trigeminal ganglia, developing central nervous system and in the mammary buds.1 Publication

    Gene expression databases

    ArrayExpressiP70324.
    BgeeiP70324.
    CleanExiMM_TBX3.
    GenevestigatoriP70324.

    Interactioni

    Subunit structurei

    Interacts with PML.By similarity

    Protein-protein interaction databases

    BioGridi203988. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP70324.
    SMRiP70324. Positions 100-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG240293.
    GeneTreeiENSGT00750000117325.
    HOGENOMiHOG000038046.
    HOVERGENiHBG000412.
    InParanoidiP70324.
    KOiK10177.
    OMAiPSQFAMG.
    OrthoDBiEOG7W9RV1.
    TreeFamiTF106341.

    Family and domain databases

    Gene3Di2.60.40.820. 1 hit.
    InterProiIPR008967. p53-like_TF_DNA-bd.
    IPR022582. TBX.
    IPR001699. TF_T-box.
    IPR018186. TF_T-box_CS.
    [Graphical view]
    PANTHERiPTHR11267. PTHR11267. 1 hit.
    PfamiPF00907. T-box. 1 hit.
    PF12598. TBX. 1 hit.
    [Graphical view]
    PRINTSiPR00937. TBOX.
    SMARTiSM00425. TBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF49417. SSF49417. 1 hit.
    PROSITEiPS01283. TBOX_1. 1 hit.
    PS01264. TBOX_2. 1 hit.
    PS50252. TBOX_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P70324-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG    50
    AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEDVE 100
    DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI 150
    LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK 200
    VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP 250
    RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA 300
    KGFRDTGNGR REKRKQLTLQ SMRVFEERHK KETSDESSSE QAAFNCFAQA 350
    SSPAVSIVGT SNLKDLCPSE AESDAEAESK EEHGPEACDA AKISTTTAEE 400
    PGRDKGSPAT RAQLFPAEPS RARDTARLDK ASPDSRHSPA TISSSTRVPG 450
    ADERRSPGRE GPVATKVDEA RAIPAKDAFA PLSVQTDATA HLAQGPLPGL 500
    GFAPGLAGQQ FFNGHPLFLH PGQFAMGGAF SSMAAGMGPL LATVSGASTG 550
    VSGLESTAMA SAAAAQGLSG ASAATLPFHL QQHVLASQGL AMSPFGSLFP 600
    YPYTYMAAAA AASTAAASSS VHRHPFLNLN SMRPRLRYSP YSIPVPVPDS 650
    SSLLATALPS MASAAGPLDG KAAALAASPA SVAVDSGSEL NSRSSTLSSG 700
    SVSLSPKLCS EKEAATSELQ SIQRLVSGLE AKPDRSCSGS P 741
    Length:741
    Mass (Da):79,160
    Last modified:July 27, 2011 - v3
    Checksum:iB502FE817EFF124D
    GO
    Isoform 2 (identifier: P70324-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         221-240: Missing.

    Show »
    Length:721
    Mass (Da):76,949
    Checksum:iF086EA71843D4D2B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti154 – 1552DI → T in AAC53107. (PubMed:8878690)Curated
    Sequence conflicti184 – 1841M → R in AAC53107. (PubMed:8878690)Curated
    Sequence conflicti306 – 3094TGNG → IGHC in AAC53107. (PubMed:8878690)Curated
    Sequence conflicti569 – 5691S → Y in BAC34999. (PubMed:16141072)Curated
    Sequence conflicti717 – 7171S → N in BAC34999. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei221 – 24020Missing in isoform 2. 2 PublicationsVSP_013377Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052453 mRNA. Translation: BAC34999.1.
    AC140675 Genomic DNA. No translation available.
    U57328 mRNA. Translation: AAC53107.1.
    U15567 mRNA. Translation: AAC52698.1.
    CCDSiCCDS19613.1. [P70324-1]
    CCDS19614.1. [P70324-2]
    RefSeqiNP_035665.2. NM_011535.3. [P70324-1]
    XP_006530339.1. XM_006530276.1. [P70324-1]
    UniGeneiMm.219139.

    Genome annotation databases

    EnsembliENSMUST00000018748; ENSMUSP00000018748; ENSMUSG00000018604. [P70324-1]
    ENSMUST00000079719; ENSMUSP00000078657; ENSMUSG00000018604. [P70324-2]
    ENSMUST00000121021; ENSMUSP00000112519; ENSMUSG00000018604. [P70324-2]
    GeneIDi21386.
    KEGGimmu:21386.
    UCSCiuc008zgu.1. mouse. [P70324-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052453 mRNA. Translation: BAC34999.1 .
    AC140675 Genomic DNA. No translation available.
    U57328 mRNA. Translation: AAC53107.1 .
    U15567 mRNA. Translation: AAC52698.1 .
    CCDSi CCDS19613.1. [P70324-1 ]
    CCDS19614.1. [P70324-2 ]
    RefSeqi NP_035665.2. NM_011535.3. [P70324-1 ]
    XP_006530339.1. XM_006530276.1. [P70324-1 ]
    UniGenei Mm.219139.

    3D structure databases

    ProteinModelPortali P70324.
    SMRi P70324. Positions 100-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203988. 3 interactions.

    PTM databases

    PhosphoSitei P70324.

    Proteomic databases

    MaxQBi P70324.
    PaxDbi P70324.
    PRIDEi P70324.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018748 ; ENSMUSP00000018748 ; ENSMUSG00000018604 . [P70324-1 ]
    ENSMUST00000079719 ; ENSMUSP00000078657 ; ENSMUSG00000018604 . [P70324-2 ]
    ENSMUST00000121021 ; ENSMUSP00000112519 ; ENSMUSG00000018604 . [P70324-2 ]
    GeneIDi 21386.
    KEGGi mmu:21386.
    UCSCi uc008zgu.1. mouse. [P70324-1 ]

    Organism-specific databases

    CTDi 6926.
    MGIi MGI:98495. Tbx3.

    Phylogenomic databases

    eggNOGi NOG240293.
    GeneTreei ENSGT00750000117325.
    HOGENOMi HOG000038046.
    HOVERGENi HBG000412.
    InParanoidi P70324.
    KOi K10177.
    OMAi PSQFAMG.
    OrthoDBi EOG7W9RV1.
    TreeFami TF106341.

    Miscellaneous databases

    NextBioi 300624.
    PROi P70324.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70324.
    Bgeei P70324.
    CleanExi MM_TBX3.
    Genevestigatori P70324.

    Family and domain databases

    Gene3Di 2.60.40.820. 1 hit.
    InterProi IPR008967. p53-like_TF_DNA-bd.
    IPR022582. TBX.
    IPR001699. TF_T-box.
    IPR018186. TF_T-box_CS.
    [Graphical view ]
    PANTHERi PTHR11267. PTHR11267. 1 hit.
    Pfami PF00907. T-box. 1 hit.
    PF12598. TBX. 1 hit.
    [Graphical view ]
    PRINTSi PR00937. TBOX.
    SMARTi SM00425. TBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49417. SSF49417. 1 hit.
    PROSITEi PS01283. TBOX_1. 1 hit.
    PS01264. TBOX_2. 1 hit.
    PS50252. TBOX_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Lung.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Evolution of mouse T-box genes by tandem duplication and cluster dispersion."
      Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I., Bollag R.J., Papaioannou V.E., Silver L.M.
      Genetics 144:249-254(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 112-313 (ISOFORM 2).
      Tissue: Embryo.
    4. "An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus."
      Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., Silver L.M.
      Nat. Genet. 7:383-389(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 185-286 (ISOFORM 2).
      Tissue: Embryo.
    5. Cited for: DEVELOPMENTAL STAGE.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-705, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiTBX3_MOUSE
    AccessioniPrimary (citable) accession number: P70324
    Secondary accession number(s): E9QM97, Q60708, Q8BWH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3