ID TBX1_MOUSE Reviewed; 479 AA. AC P70323; Q60706; Q99MP0; Q99P22; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 20-DEC-2005, sequence version 2. DT 24-JAN-2024, entry version 174. DE RecName: Full=T-box transcription factor TBX1; DE Short=T-box protein 1; DE AltName: Full=Testis-specific T-box protein; GN Name=Tbx1 {ECO:0000303|PubMed:11412027, ECO:0000312|MGI:MGI:98493}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY. RC STRAIN=Swiss Webster / NIH; RX PubMed=11412027; DOI=10.1006/dbio.2001.0283; RA Garg V., Yamagishi C., Hu T., Kathiriya I.S., Yamagishi H., Srivastava D.; RT "Tbx1, a DiGeorge syndrome candidate gene, is regulated by sonic hedgehog RT during pharyngeal arch development."; RL Dev. Biol. 235:62-73(2001). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=11242049; DOI=10.1038/35065105; RA Lindsay E.A., Vitelli F., Su H., Morishima M., Huynh T., Pramparo T., RA Jurecic V., Ogunrinu G., Sutherland H.F., Scambler P.J., Bradley A., RA Baldini A.; RT "Tbx1 haploinsufficiency in the DiGeorge syndrome region causes aortic arch RT defects in mice."; RL Nature 410:97-101(2001). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA] OF 108-291 (ISOFORMS 1/2). RC TISSUE=Embryo; RX PubMed=8878690; DOI=10.1093/genetics/144.1.249; RA Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I., RA Bollag R.J., Papaioannou V.E., Silver L.M.; RT "Evolution of mouse T-box genes by tandem duplication and cluster RT dispersion."; RL Genetics 144:249-254(1996). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA] OF 183-267 (ISOFORMS 1/2). RC TISSUE=Embryo; RX PubMed=7920656; DOI=10.1038/ng0794-383; RA Bollag R.J., Siegfried Z., Cebra-Thomas J.A., Garvey N., Davison E.M., RA Silver L.M.; RT "An ancient family of embryonically expressed mouse genes sharing a RT conserved protein motif with the T locus."; RL Nat. Genet. 7:383-389(1994). RN [5] RP DEVELOPMENTAL STAGE. RX PubMed=8853987; RX DOI=10.1002/(sici)1097-0177(199608)206:4<379::aid-aja4>3.0.co;2-f; RA Chapman D.L., Garvey N., Hancock S., Alexiou M., Agulnik S.I., RA Gibson-Brown J.J., Cebra-Thomas J., Bollag R.J., Silver L.M., RA Papaioannou V.E.; RT "Expression of the T-box family genes, Tbx1-Tbx5, during early mouse RT development."; RL Dev. Dyn. 206:379-390(1996). RN [6] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=11239417; DOI=10.1016/s0092-8674(01)00247-1; RA Merscher S., Funke B., Epstein J.A., Heyer J., Puech A., Lu M.M., RA Xavier R.J., Demay M.B., Russell R.G., Factor S., Tokooya K., Jore B.S., RA Lopez M., Pandita R.K., Lia M., Carrion D., Xu H., Schorle H., Kobler J.B., RA Scambler P., Wynshaw-Boris A., Skoultchi A.I., Morrow B.E., RA Kucherlapati R.; RT "TBX1 is responsible for cardiovascular defects in velo-cardio- RT facial/DiGeorge syndrome."; RL Cell 104:619-629(2001). RN [7] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=11242110; DOI=10.1038/85845; RA Jerome L.A., Papaioannou V.E.; RT "DiGeorge syndrome phenotype in mice mutant for the T-box gene, Tbx1."; RL Nat. Genet. 27:286-291(2001). RN [8] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=15385444; DOI=10.1093/hmg/ddh304; RA Kelly R.G., Jerome-Majewska L.A., Papaioannou V.E.; RT "The del22q11.2 candidate gene Tbx1 regulates branchiomeric myogenesis."; RL Hum. Mol. Genet. 13:2829-2840(2004). RN [9] RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF PHE-137; GLY-299 AND RP 417-ARG--TYR-421. RX PubMed=15703190; DOI=10.1093/hmg/ddi081; RA Stoller J.Z., Epstein J.A.; RT "Identification of a novel nuclear localization signal in Tbx1 that is RT deleted in DiGeorge syndrome patients harboring the 1223delC mutation."; RL Hum. Mol. Genet. 14:885-892(2005). RN [10] RP FUNCTION, AND INTERACTION WITH NKX2-5. RX PubMed=16556915; DOI=10.1242/dev.02309; RA Nowotschin S., Liao J., Gage P.J., Epstein J.A., Campione M., Morrow B.E.; RT "Tbx1 affects asymmetric cardiac morphogenesis by regulating Pitx2 in the RT secondary heart field."; RL Development 133:1565-1573(2006). RN [11] RP INTERACTION WITH DSCR6, AND DEVELOPMENTAL STAGE. RX PubMed=21177346; DOI=10.1242/dev.054056; RA Okubo T., Kawamura A., Takahashi J., Yagi H., Morishima M., Matsuoka R., RA Takada S.; RT "Ripply3, a Tbx1 repressor, is required for development of the pharyngeal RT apparatus and its derivatives in mice."; RL Development 138:339-348(2011). RN [12] RP FUNCTION, AND TISSUE SPECIFICITY. RX PubMed=22495305; DOI=10.1038/nature10940; RA Chen T., Heller E., Beronja S., Oshimori N., Stokes N., Fuchs E.; RT "An RNA interference screen uncovers a new molecule in stem cell self- RT renewal and long-term regeneration."; RL Nature 485:104-108(2012). RN [13] RP FUNCTION. RX PubMed=31412026; DOI=10.1371/journal.pgen.1008301; RA Hasten E., Morrow B.E.; RT "Tbx1 and Foxi3 genetically interact in the pharyngeal pouch endoderm in a RT mouse model for 22q11.2 deletion syndrome."; RL PLoS Genet. 15:e1008301-e1008301(2019). CC -!- FUNCTION: Transcription factor that plays a key role in cardiovascular CC development by promoting pharyngeal arch segmentation during embryonic CC development (PubMed:11412027, PubMed:11242049, PubMed:11239417, CC PubMed:11242110, PubMed:15703190, PubMed:16556915). Also involved in CC craniofacial muscle development (PubMed:15385444). Together with NKX2- CC 5, acts as a regulator of asymmetric cardiac morphogenesis by promoting CC expression of PITX2 (PubMed:16556915). Acts upstream of TBX1 for the CC formation of the thymus and parathyroid glands from the third CC pharyngeal pouch (PubMed:31412026). Required for hair follicle stem CC cell self-renewal (PubMed:22495305). Binds to the palindromic T site CC 5'-TTCACACCTAGGTGTGAA-3' DNA sequence (By similarity). CC {ECO:0000250|UniProtKB:O43435, ECO:0000269|PubMed:11239417, CC ECO:0000269|PubMed:11242049, ECO:0000269|PubMed:11242110, CC ECO:0000269|PubMed:11412027, ECO:0000269|PubMed:15385444, CC ECO:0000269|PubMed:15703190, ECO:0000269|PubMed:16556915, CC ECO:0000269|PubMed:22495305, ECO:0000269|PubMed:31412026}. CC -!- SUBUNIT: Binds DNA as a dimer (By similarity). Interacts with DSCR6 CC (PubMed:21177346). Interacts with NKX2-5 (PubMed:16556915). CC {ECO:0000250|UniProtKB:O43435, ECO:0000269|PubMed:16556915, CC ECO:0000269|PubMed:21177346}. CC -!- INTERACTION: CC P70323; P28359: Hoxd10; NbExp=3; IntAct=EBI-13635846, EBI-445929; CC P70323; Q61466: Smarcd1; NbExp=3; IntAct=EBI-13635846, EBI-371529; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201, CC ECO:0000269|PubMed:15703190}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=P70323-1; Sequence=Displayed; CC Name=2; CC IsoId=P70323-2; Sequence=VSP_016760; CC -!- TISSUE SPECIFICITY: Expressed in skeletal muscle, lung and testis CC (PubMed:11412027). Highly expressed in hair follicle stem cell, but not CC in terminally differentiating cells (PubMed:22495305). CC {ECO:0000269|PubMed:11412027, ECO:0000269|PubMed:22495305}. CC -!- DEVELOPMENTAL STAGE: In the developing embryo, first expressed in the CC mesoderm at day 7.5. Expressed in the pharyngeal endoderm and the CC mesodermal cores of the pharyngeal arches at 8.5 dpc. At day 9.5, CC primarily expressed in the head region, specifically the mesenchyme and CC epithelium of the pharyngeal region and the otic vesicle epithelium. By CC day 12.5, expression is still observed in the latter as well as in the CC tongue mesenchyme, tooth buds and branching lung epithelium. CC {ECO:0000269|PubMed:21177346, ECO:0000269|PubMed:8853987}. CC -!- DISRUPTION PHENOTYPE: Mice die at birth with hypoplastic and CC intermittent missing craniofacial muscles, cleft palate, absent thymus CC and parathyroid glands, as well as a persistent truncus arteriosus CC (PTA) with a ventricular septal defect (VSD) (PubMed:11242049, CC PubMed:11239417, PubMed:11242110, PubMed:15385444). The first arch CC forms in but the distal pharyngeal arch fails to become segmented, CC thereby explaining, why the pharyngeal arch derived structures are CC malformed (PubMed:11242049, PubMed:11239417, PubMed:11242110). CC Conditional deletion in the skin epithelium leads to delayed tissue CC regeneration due to progressive depletion of stem cells CC (PubMed:22495305). {ECO:0000269|PubMed:11239417, CC ECO:0000269|PubMed:11242049, ECO:0000269|PubMed:11242110, CC ECO:0000269|PubMed:15385444, ECO:0000269|PubMed:22495305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF349658; AAK14407.1; -; mRNA. DR EMBL; AF326960; AAG61088.1; -; mRNA. DR EMBL; U57327; AAC53106.1; -; mRNA. DR EMBL; U15565; AAC52696.1; -; mRNA. DR CCDS; CCDS28023.1; -. [P70323-2] DR CCDS; CCDS88897.1; -. [P70323-1] DR RefSeq; NP_035662.1; NM_011532.2. DR AlphaFoldDB; P70323; -. DR SMR; P70323; -. DR BioGRID; 203984; 5. DR IntAct; P70323; 3. DR STRING; 10090.ENSMUSP00000156061; -. DR iPTMnet; P70323; -. DR PhosphoSitePlus; P70323; -. DR PaxDb; 10090-ENSMUSP00000009241; -. DR ProteomicsDB; 263014; -. [P70323-1] DR ProteomicsDB; 263015; -. [P70323-2] DR DNASU; 21380; -. DR GeneID; 21380; -. DR KEGG; mmu:21380; -. DR AGR; MGI:98493; -. DR CTD; 6899; -. DR MGI; MGI:98493; Tbx1. DR eggNOG; KOG3586; Eukaryota. DR InParanoid; P70323; -. DR OrthoDB; 5323209at2759; -. DR PhylomeDB; P70323; -. DR BioGRID-ORCS; 21380; 2 hits in 80 CRISPR screens. DR ChiTaRS; Litaf; mouse. DR PRO; PR:P70323; -. DR Proteomes; UP000000589; Unplaced. DR RNAct; P70323; Protein. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:0003677; F:DNA binding; ISO:MGI. DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0001162; F:RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB. DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB. DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI. DR GO; GO:0036305; P:ameloblast differentiation; IMP:MGI. DR GO; GO:0001525; P:angiogenesis; IMP:MGI. DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI. DR GO; GO:0035909; P:aorta morphogenesis; IMP:MGI. DR GO; GO:0048844; P:artery morphogenesis; IMP:MGI. DR GO; GO:0001568; P:blood vessel development; IMP:MGI. DR GO; GO:0048514; P:blood vessel morphogenesis; IMP:MGI. DR GO; GO:0001974; P:blood vessel remodeling; IGI:MGI. DR GO; GO:0001569; P:branching involved in blood vessel morphogenesis; TAS:DFLAT. DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central. DR GO; GO:0008283; P:cell population proliferation; IMP:MGI. DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IMP:UniProtKB. DR GO; GO:0071300; P:cellular response to retinoic acid; IEP:UniProtKB. DR GO; GO:0090103; P:cochlea morphogenesis; IMP:MGI. DR GO; GO:0060982; P:coronary artery morphogenesis; IMP:MGI. DR GO; GO:1904888; P:cranial skeletal system development; IGI:MGI. DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI. DR GO; GO:0042471; P:ear morphogenesis; IMP:MGI. DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; IMP:MGI. DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; ISS:UniProtKB. DR GO; GO:0070166; P:enamel mineralization; IMP:MGI. DR GO; GO:0050673; P:epithelial cell proliferation; IMP:MGI. DR GO; GO:0060325; P:face morphogenesis; IMP:MGI. DR GO; GO:0060022; P:hard palate development; IMP:MGI. DR GO; GO:0007507; P:heart development; IMP:UniProtKB. DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI. DR GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI. DR GO; GO:0001945; P:lymph vessel development; IMP:MGI. DR GO; GO:0097152; P:mesenchymal cell apoptotic process; IMP:UniProtKB. DR GO; GO:0010463; P:mesenchymal cell proliferation; IDA:MGI. DR GO; GO:0007498; P:mesoderm development; IMP:MGI. DR GO; GO:0042474; P:middle ear morphogenesis; IMP:MGI. DR GO; GO:0042693; P:muscle cell fate commitment; IMP:MGI. DR GO; GO:0007517; P:muscle organ development; IMP:MGI. DR GO; GO:0048644; P:muscle organ morphogenesis; IGI:MGI. DR GO; GO:0060415; P:muscle tissue morphogenesis; IMP:MGI. DR GO; GO:0045596; P:negative regulation of cell differentiation; IMP:MGI. DR GO; GO:2001054; P:negative regulation of mesenchymal cell apoptotic process; IGI:UniProtKB. DR GO; GO:0010656; P:negative regulation of muscle cell apoptotic process; ISO:MGI. DR GO; GO:0046426; P:negative regulation of receptor signaling pathway via JAK-STAT; ISO:MGI. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:BHF-UCL. DR GO; GO:0001755; P:neural crest cell migration; IMP:MGI. DR GO; GO:0048665; P:neuron fate specification; IMP:MGI. DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI. DR GO; GO:0071600; P:otic vesicle morphogenesis; IMP:MGI. DR GO; GO:0042473; P:outer ear morphogenesis; IMP:MGI. DR GO; GO:0003151; P:outflow tract morphogenesis; IMP:MGI. DR GO; GO:0003148; P:outflow tract septum morphogenesis; IMP:UniProtKB. DR GO; GO:0060017; P:parathyroid gland development; IMP:UniProtKB. DR GO; GO:0007389; P:pattern specification process; IMP:MGI. DR GO; GO:0060037; P:pharyngeal system development; IMP:UniProtKB. DR GO; GO:0060045; P:positive regulation of cardiac muscle cell proliferation; ISO:MGI. DR GO; GO:1902808; P:positive regulation of cell cycle G1/S phase transition; ISO:MGI. DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:MGI. DR GO; GO:0050679; P:positive regulation of epithelial cell proliferation; IMP:UniProtKB. DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI. DR GO; GO:0043410; P:positive regulation of MAPK cascade; IMP:UniProtKB. DR GO; GO:0002053; P:positive regulation of mesenchymal cell proliferation; IMP:UniProtKB. DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IMP:UniProtKB. DR GO; GO:0072513; P:positive regulation of secondary heart field cardioblast proliferation; IDA:MGI. DR GO; GO:2001037; P:positive regulation of tongue muscle cell differentiation; IMP:UniProtKB. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:2000027; P:regulation of animal organ morphogenesis; IMP:MGI. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IGI:MGI. DR GO; GO:0048384; P:retinoic acid receptor signaling pathway; IMP:UniProtKB. DR GO; GO:0048752; P:semicircular canal morphogenesis; IGI:MGI. DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI. DR GO; GO:0035176; P:social behavior; IMP:MGI. DR GO; GO:0060023; P:soft palate development; ISS:UniProtKB. DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:UniProtKB. DR GO; GO:0048538; P:thymus development; IMP:UniProtKB. DR GO; GO:0030878; P:thyroid gland development; IMP:MGI. DR GO; GO:0043587; P:tongue morphogenesis; IMP:UniProtKB. DR GO; GO:0021644; P:vagus nerve morphogenesis; IMP:MGI. DR CDD; cd20187; T-box_TBX1_10-like; 1. DR Gene3D; 2.60.40.820; Transcription factor, T-box; 1. DR InterPro; IPR008967; p53-like_TF_DNA-bd_sf. DR InterPro; IPR046360; T-box_DNA-bd. DR InterPro; IPR036960; T-box_sf. DR InterPro; IPR001699; TF_T-box. DR InterPro; IPR018186; TF_T-box_CS. DR PANTHER; PTHR11267; T-BOX PROTEIN-RELATED; 1. DR PANTHER; PTHR11267:SF104; T-BOX TRANSCRIPTION FACTOR TBX1; 1. DR Pfam; PF00907; T-box; 1. DR PRINTS; PR00937; TBOX. DR SMART; SM00425; TBOX; 1. DR SUPFAM; SSF49417; p53-like transcription factors; 1. DR PROSITE; PS01283; TBOX_1; 1. DR PROSITE; PS01264; TBOX_2; 1. DR PROSITE; PS50252; TBOX_3; 1. PE 1: Evidence at protein level; KW Alternative splicing; Developmental protein; DNA-binding; Nucleus; KW Reference proteome; Transcription; Transcription regulation. FT CHAIN 1..479 FT /note="T-box transcription factor TBX1" FT /id="PRO_0000184424" FT DNA_BIND 108..286 FT /note="T-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201" FT REGION 15..86 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 311..398 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 415..426 FT /note="Nuclear localization signal" FT /evidence="ECO:0000269|PubMed:15703190" FT COMPBIAS 29..62 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 325..339 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT VAR_SEQ 1..11 FT /note="MHFSTVTRDME -> MISAVSSPWLTQLSHFCDVA (in isoform 2)" FT /evidence="ECO:0000305" FT /id="VSP_016760" FT MUTAGEN 137 FT /note="F->Y: Does not affect transcription factor activity FT or nuclear localization." FT /evidence="ECO:0000269|PubMed:15703190" FT MUTAGEN 299 FT /note="G->S: Does not affect transcription factor activity FT or nuclear localization." FT /evidence="ECO:0000269|PubMed:15703190" FT MUTAGEN 417..421 FT /note="RPAPY->APAPA: Abolished nuclear localization." FT /evidence="ECO:0000269|PubMed:15703190" FT CONFLICT 174 FT /note="A -> R (in Ref. 3; AAC53106)" FT /evidence="ECO:0000305" FT CONFLICT 183..184 FT /note="HY -> YI (in Ref. 4; AAC52696)" FT /evidence="ECO:0000305" FT CONFLICT 217 FT /note="Q -> H (in Ref. 2; AAG61088, 3; AAC53106 and 4; FT AAC52696)" FT /evidence="ECO:0000305" FT CONFLICT 236 FT /note="D -> A (in Ref. 3; AAC53106 and 4; AAC52696)" FT /evidence="ECO:0000305" FT CONFLICT 245 FT /note="A -> E (in Ref. 3; AAC53106 and 4; AAC52696)" FT /evidence="ECO:0000305" SQ SEQUENCE 479 AA; 51668 MW; F6FE34621B2E7164 CRC64; MHFSTVTRDM EAFAASSLSG LGSPSPGADP FGPREPPPPR YDPCAAVPGA PGPPPPRAYP FAPAPGAAGS SAAESEGPGA SRAAAVKAPV KKNPKVASVS VQLEMKALWD EFNQLGTEMI VTKAGRRMFP TFQVKLFGMD PMADYMLLMD FVPVDDKRYR YAFHSSSWLV AGKADPATPG RVHYHPDSPA KGAQWMKQIV SFDKLKLTNN LLDDNGQIIL NSMHRYQPRF HVVYVDPRKD SEKYAEENFK TFVFEETRFT AVTAYQNHRI TQLKIASNPF AKGFRDCDPE DWPRNHRPGA LPLVSAFARS RNPVASPTQP NGSDKDAAEA RREFDRDSGP AALGDATHPP QLLARVLSPA LPGPGGLVPL PGGSGGRHSP PHADLRLEAP GASEPLHHHP YKYPAAAYDH YLGAKSRPAP YPLPGLRGHG YHPHAHPHAH PHHHHHPAVN PAAAAAAAAA ANVYSSAAAP PGAYDYCPR //