P70318 (TIAR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolysin TIAR

Alternative name(s):
TIA-1-related protein

Gene names

Name:

Tial1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis By similarity.
Subcellular location	Cytoplasm. Nucleus. Cytoplasmic granule. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules. Ref.2
Tissue specificity	Expressed both in primordial germ cells (PGCs) and in neighboring somatic cells. Ref.2
Post-translational modification	Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA By similarity.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW germ cell development Inferred from mutant phenotype PubMed 9482885. Source: MGI positive regulation of cell proliferation Inferred from mutant phenotype PubMed 9482885. Source: MGI stem cell division Inferred from mutant phenotype PubMed 9482885. Source: MGI
Cellular_component	cytoplasmic stress granule Inferred from direct assay Ref.2. Source: UniProtKB nucleus Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	AU-rich element binding Inferred from direct assay PubMed 10921895. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Nucleolysin TIAR		PRO_0000081979
Regions
	Domain	9 – 102	94	RRM 1
	Domain	114 – 192	79	RRM 2
	Domain	222 – 294	73	RRM 3
Amino acid modifications
	Modified residue	139	1	N6-acetyllysine By similarity
	Modified residue	218	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70318 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 66E6AF14B9EBE77A	FASTA	392	43,389
	10 20 30 40 50 60 MMEDDGQPRT LYVGNLSRDV TEVLILQLFS QIGPCKSCKM ITEQPDSRRV NSSVGFSVLQ 70 80 90 100 110 120 HTSNDPYCFV EFYEHRDAAA ALAAMNGRKI LGKEVKVNWA TTPSSQKKDT SNHFHVFVGD 130 140 150 160 170 180 LSPEITTEDI KSAFAPFGKI SDARVVKDMA TGKSKGYGFV SFYNKLDAEN AIVHMGGQWL 190 200 210 220 230 240 GGRQIRTNWA TRKPPAPKST QETNTKQLRF EDVVNQSSPK NCTVYCGGIA SGLTDQLMRQ 250 260 270 280 290 300 TFSPFGQIME IRVFPEKGYS FVRFSTHESA AHAIVSVNGT TIEGHVVKCY WGKESPDMTK 310 320 330 340 350 360 NFQQVDYSQW GQWSQVYGNP QQYGQYMANG WQVPPYGVYG QPWNQQGFGV DQSPSAAWMG 370 380 390 GFGAQPPQGQ APPPVIPPPN QAGYGMASFP TQ

References

[1]	"Structure, tissue distribution and genomic organization of the murine RRM-type RNA binding proteins TIA-1 and TIAR." Beck A.R.P., Medley O.G., O'Brien S., Anderson P., Streuli M. Nucleic Acids Res. 24:3829-3836(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3." Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M. Reproduction 139:381-393(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U55861 mRNA. Translation: AAC52870.1.
IPI	IPI00108085.
PIR	S72436.
RefSeq	NP_033409.1. NM_009383.2.
UniGene	Mm.242072.
3D structure databases
ProteinModelPortal	P70318.
SMR	P70318. Positions 1-299.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70318. 1 interaction.
PTM databases
PhosphoSite	P70318.
Proteomic databases
PaxDb	P70318.
PRIDE	P70318.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106226; ENSMUSP00000101833; ENSMUSG00000030846.
GeneID	21843.
KEGG	mmu:21843.
Organism-specific databases
CTD	7073.
MGI	MGI:107913. Tial1.
Phylogenomic databases
eggNOG	COG0724.
HOGENOM	HOG000206748.
HOVERGEN	HBG105006.
InParanoid	P70318.
KO	K13201.
OMA	NGSKQLR.
OrthoDB	EOG4B5P59.
Gene expression databases
ArrayExpress	P70318.
Bgee	P70318.
Genevestigator	P70318.
GermOnline	ENSMUSG00000030846. Mus musculus.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
SMART	SM00360. RRM. 3 hits. [Graphical view]
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TIAL1. mouse.
NextBio	301310.
SOURCE	Search...

Entry information

Entry name

TIAR_MOUSE

Accession

Primary (citable) accession number: P70318

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program