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P70315

- WASP_MOUSE

UniProt

P70315 - WASP_MOUSE

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Protein

Wiskott-Aldrich syndrome protein homolog

Gene

Was

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.

GO - Molecular functioni

  1. identical protein binding Source: MGI

GO - Biological processi

  1. actin filament-based movement Source: MGI
  2. actin filament polymerization Source: MGI
  3. actin polymerization or depolymerization Source: MGI
  4. endosomal transport Source: MGI
  5. immune response Source: Ensembl
  6. positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  7. T cell activation Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein homolog
Short name:
WASp
Gene namesi
Name:Was
Synonyms:Wasp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:105059. Was.

Subcellular locationi

GO - Cellular componenti

  1. cell-cell junction Source: MGI
  2. cytoskeleton Source: UniProtKB-KW
  3. cytosol Source: Reactome
  4. extracellular vesicular exosome Source: Ensembl
  5. vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Wiskott-Aldrich syndrome protein homologPRO_0000188991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei293 – 2931Phosphotyrosine; alternate2 Publications
Modified residuei293 – 2931Phosphotyrosine; by FYN and HCK; alternateBy similarity
Modified residuei501 – 5011Phosphoserine1 Publication
Modified residuei502 – 5021Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70315.
PRIDEiP70315.

PTM databases

PhosphoSiteiP70315.

Expressioni

Gene expression databases

BgeeiP70315.
CleanExiMM_WAS.
ExpressionAtlasiP70315. baseline and differential.
GenevestigatoriP70315.

Interactioni

Subunit structurei

Interacts with CDC42, RAC, NCK, FYN, HCK, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA (By similarity). Interacts with NCK1 (via SH3 domains).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ipo5Q8BKC58EBI-644195,EBI-643605
SrcP054802EBI-644195,EBI-298680
Wipf1Q8K1I73EBI-644195,EBI-644216

Protein-protein interaction databases

BioGridi204544. 7 interactions.
DIPiDIP-36426N.
IntActiP70315. 12 interactions.
MINTiMINT-1532664.

Structurei

3D structure databases

ProteinModelPortaliP70315.
SMRiP70315. Positions 37-159, 244-311, 482-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 150110WH1PROSITE-ProRule annotationAdd
BLAST
Domaini240 – 25314CRIBPROSITE-ProRule annotationAdd
BLAST
Repeati354 – 36310GRSGPLPPXP motif 1
Repeati393 – 40210GRSGPLPPXP motif 2
Domaini448 – 46518WH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi162 – 1676Poly-Pro
Compositional biasi314 – 3218Poly-Pro
Compositional biasi324 – 34118Poly-GlyAdd
BLAST
Compositional biasi368 – 3736Poly-Pro
Compositional biasi376 – 3794Poly-Pro
Compositional biasi384 – 3907Poly-Pro
Compositional biasi397 – 4037Poly-Pro
Compositional biasi408 – 42417Poly-ProAdd
BLAST
Compositional biasi503 – 52018Asp/Glu-rich (acidic)Add
BLAST

Domaini

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270974.
HOVERGENiHBG000222.
InParanoidiP70315.
KOiK05747.
OMAiTPANEER.
OrthoDBiEOG7FJH20.
PhylomeDBiP70315.
TreeFamiTF316736.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 2 hits.
InterProiIPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR027641. WASP.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12779:SF2. PTHR12779:SF2. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70315-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT
60 70 80 90 100
TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE
110 120 130 140 150
QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR
160 170 180 190 200
NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV
210 220 230 240 250
TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS
260 270 280 290 300
HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG
310 320 330 340 350
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG
360 370 380 390 400
SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP
410 420 430 440 450
PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG
460 470 480 490 500
ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH
510 520
SSDEGEDQTG EDEEDDEWDD
Length:520
Mass (Da):54,192
Last modified:February 1, 1997 - v1
Checksum:i9C223733C59F0C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA. Translation: AAC52556.1.
CCDSiCCDS29984.1.
RefSeqiNP_033541.1. NM_009515.2.
UniGeneiMm.4735.

Genome annotation databases

EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165.
GeneIDi22376.
KEGGimmu:22376.
UCSCiuc009sns.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA. Translation: AAC52556.1 .
CCDSi CCDS29984.1.
RefSeqi NP_033541.1. NM_009515.2.
UniGenei Mm.4735.

3D structure databases

ProteinModelPortali P70315.
SMRi P70315. Positions 37-159, 244-311, 482-510.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204544. 7 interactions.
DIPi DIP-36426N.
IntActi P70315. 12 interactions.
MINTi MINT-1532664.

PTM databases

PhosphoSitei P70315.

Proteomic databases

PaxDbi P70315.
PRIDEi P70315.

Protocols and materials databases

DNASUi 22376.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033505 ; ENSMUSP00000033505 ; ENSMUSG00000031165 .
GeneIDi 22376.
KEGGi mmu:22376.
UCSCi uc009sns.2. mouse.

Organism-specific databases

CTDi 7454.
MGIi MGI:105059. Was.

Phylogenomic databases

eggNOGi NOG270974.
HOVERGENi HBG000222.
InParanoidi P70315.
KOi K05747.
OMAi TPANEER.
OrthoDBi EOG7FJH20.
PhylomeDBi P70315.
TreeFami TF316736.

Enzyme and pathway databases

Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 302721.
PROi P70315.
SOURCEi Search...

Gene expression databases

Bgeei P70315.
CleanExi MM_WAS.
ExpressionAtlasi P70315. baseline and differential.
Genevestigatori P70315.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.90.810.10. 2 hits.
InterProi IPR000095. CRIB_dom.
IPR011993. PH_like_dom.
IPR027641. WASP.
IPR011026. WASP_C.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
[Graphical view ]
PANTHERi PTHR12779:SF2. PTHR12779:SF2. 1 hit.
Pfami PF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view ]
SUPFAMi SSF47912. SSF47912. 2 hits.
PROSITEi PS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP) gene is highly conserved and maps near the scurfy (sf) mutation on the X chromosome."
    Derry J.M.J., Wiedemann P., Blair P., Wang Y., Kerns J.A., Lemahieu V., Godfrey V.L., Wilkinson J.E., Francke U.
    Genomics 29:471-477(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling."
    Quilliam L.A., Lambert Q.T., Mickelson-Young L.A., Westwick J.K., Sparks A.B., Kay B.K., Jenkins N.A., Gilbert D.J., Copeland N.G., Der C.J.
    J. Biol. Chem. 271:28772-28776(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCK1.
  3. "Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein."
    Wu Y., Spencer S.D., Lasky L.A.
    J. Biol. Chem. 273:5765-5770(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PSTPIP1.
  4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293; SER-501 AND SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWASP_MOUSE
AccessioniPrimary (citable) accession number: P70315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3