Wiskott-Aldrich syndrome protein homolog

Contribute

Send feedback

Read comments (?) or add your own

P70315 (WASP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Wiskott-Aldrich syndrome protein homolog
Short name=WASp

Gene names

Name:	Was
Synonyms:	Wasp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.
Subunit structure	Interacts with CDC42, RAC, NCK, FYN, HCK, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA By similarity. Interacts with NCK1 (via SH3 domains). Ref.2 Ref.3
Subcellular location	Cytoplasm › cytoskeleton.
Domain	The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand. The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Post-translational modification	Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation By similarity.
Sequence similarities	Contains 1 CRIB domain. Contains 1 WH1 domain. Contains 1 WH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell activation Inferred from mutant phenotype PubMed 11070165. Source: MGI actin filament polymerization Inferred from direct assay PubMed 12147689. Source: MGI actin filament-based movement Inferred from direct assay PubMed 12147689. Source: MGI endosomal transport Inferred from direct assay PubMed 12147689. Source: MGI immune response Inferred from electronic annotation. Source: Compara
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW vesicle membrane Inferred from direct assay PubMed 12147689. Source: MGI
Molecular_function	identical protein binding Inferred from direct assay PubMed 12147689. Source: MGI small GTPase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
Ipo5	Q8BKC5	8	EBI-644195,EBI-643605
Src	P05480	2	EBI-644195,EBI-298680
Wipf1	Q8K1I7	3	EBI-644195,EBI-644216

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 520

520

Wiskott-Aldrich syndrome protein homolog

PRO_0000188991

Regions

Domain

41 – 150

110

WH1

Domain

240 – 253

CRIB

Repeat

354 – 363

GRSGPLPPXP motif 1

Repeat

393 – 402

GRSGPLPPXP motif 2

Domain

448 – 465

WH2

Compositional bias

162 – 167

Poly-Pro

Compositional bias

314 – 321

Poly-Pro

Compositional bias

324 – 341

Poly-Gly

Compositional bias

368 – 373

Poly-Pro

Compositional bias

376 – 379

Poly-Pro

Compositional bias

384 – 390

Poly-Pro

Compositional bias

397 – 403

Poly-Pro

Compositional bias

408 – 424

Poly-Pro

Compositional bias

503 – 520

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

293

Phosphotyrosine; by FYN and HCK By similarity

Modified residue

501

Phosphoserine Ref.5

Modified residue

502

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P70315 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 9C223733C59F0C8A
Show »

FASTA

520

54,192

        10         20         30         40         50         60 
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT TVVQLYLALP 

        70         80         90        100        110        120 
PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE QELYSQLVYL TPTPFFHTFA 

       130        140        150        160        170        180 
GDDCQVGLNF ADESEAQAFR ALVQEKIQKR NQRQSGERRQ LPPPPAPINE ERRGGLPPVP 

       190        200        210        220        230        240 
PHPGGDHGGP SGGPLSLGLV TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI 

       250        260        270        280        290        300 
GAPSGFKHVS HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG 

       310        320        330        340        350        360 
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG SNKGRSGPLP 

       370        380        390        400        410        420 
PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP PLPGAGGPPA PPPPPPPPPP 

       430        440        450        460        470        480 
PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ 

       490        500        510        520 
SEGLVGALMH VMQKRSRVIH SSDEGEDQTG EDEEDDEWDD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP) gene is highly conserved and maps near the scurfy (sf) mutation on the X chromosome." Derry J.M.J., Wiedemann P., Blair P., Wang Y., Kerns J.A., Lemahieu V., Godfrey V.L., Wilkinson J.E., Francke U. Genomics 29:471-477(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling." Quilliam L.A., Lambert Q.T., Mickelson-Young L.A., Westwick J.K., Sparks A.B., Kay B.K., Jenkins N.A., Gilbert D.J., Copeland N.G., Der C.J. J. Biol. Chem. 271:28772-28776(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NCK1.
[3]	"Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein." Wu Y., Spencer S.D., Lasky L.A. J. Biol. Chem. 273:5765-5770(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PSTPIP1.
[4]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293, MASS SPECTROMETRY. Tissue: Mast cell.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293; SER-501 AND SER-502, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U54788 mRNA. Translation: AAC52556.1.
IPI	IPI00108084.
RefSeq	NP_033541.1. NM_009515.2.
UniGene	Mm.4735.
3D structure databases
ProteinModelPortal	P70315.
SMR	P70315. Positions 37-159, 244-311, 482-510.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70315. 12 interactions.
PTM databases
PhosphoSite	P70315.
Proteomic databases
PaxDb	P70315.
PRIDE	P70315.
Protocols and materials databases
DNASU	22376.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165.
GeneID	22376.
KEGG	mmu:22376.
Organism-specific databases
CTD	7454.
MGI	MGI:105059. Was.
Phylogenomic databases
eggNOG	NOG270974.
HOVERGEN	HBG000222.
InParanoid	P70315.
KO	K05747.
OrthoDB	EOG4N5VXF.
Gene expression databases
ArrayExpress	P70315.
Bgee	P70315.
CleanEx	MM_WAS.
Genevestigator	P70315.
GermOnline	ENSMUSG00000031165. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000697. EVH1. IPR000095. PAK_box_Rho-bd. IPR011993. PH_like_dom. IPR011026. WASP_C. IPR003124. WH2_dom. [Graphical view]
Pfam	PF00786. PBD. 1 hit. PF00568. WH1. 1 hit. PF02205. WH2. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. SM00461. WH1. 1 hit. SM00246. WH2. 1 hit. [Graphical view]
SUPFAM	SSF47912. WASP_C. 2 hits.
PROSITE	PS50108. CRIB. 1 hit. PS50229. WH1. 1 hit. PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302721.
SOURCE	Search...

Entry information

Entry name

WASP_MOUSE

Accession

Primary (citable) accession number: P70315

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents