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P70315

- WASP_MOUSE

UniProt

P70315 - WASP_MOUSE

Protein

Wiskott-Aldrich syndrome protein homolog

Gene

Was

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.

    GO - Molecular functioni

    1. identical protein binding Source: MGI
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. actin filament-based movement Source: MGI
    2. actin filament polymerization Source: MGI
    3. actin polymerization or depolymerization Source: MGI
    4. endosomal transport Source: MGI
    5. immune response Source: Ensembl
    6. positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
    7. T cell activation Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wiskott-Aldrich syndrome protein homolog
    Short name:
    WASp
    Gene namesi
    Name:Was
    Synonyms:Wasp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:105059. Was.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. cytoskeleton Source: UniProtKB-SubCell
    3. cytosol Source: Reactome
    4. vesicle membrane Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Wiskott-Aldrich syndrome protein homologPRO_0000188991Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei293 – 2931Phosphotyrosine; alternate2 Publications
    Modified residuei293 – 2931Phosphotyrosine; by FYN and HCK; alternateBy similarity
    Modified residuei501 – 5011Phosphoserine1 Publication
    Modified residuei502 – 5021Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP70315.
    PRIDEiP70315.

    PTM databases

    PhosphoSiteiP70315.

    Expressioni

    Gene expression databases

    ArrayExpressiP70315.
    BgeeiP70315.
    CleanExiMM_WAS.
    GenevestigatoriP70315.

    Interactioni

    Subunit structurei

    Interacts with CDC42, RAC, NCK, FYN, HCK, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA By similarity. Interacts with NCK1 (via SH3 domains).By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ipo5Q8BKC58EBI-644195,EBI-643605
    SrcP054802EBI-644195,EBI-298680
    Wipf1Q8K1I73EBI-644195,EBI-644216

    Protein-protein interaction databases

    BioGridi204544. 7 interactions.
    DIPiDIP-36426N.
    IntActiP70315. 12 interactions.
    MINTiMINT-1532664.

    Structurei

    3D structure databases

    ProteinModelPortaliP70315.
    SMRiP70315. Positions 37-159, 244-311, 482-510.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 150110WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini240 – 25314CRIBPROSITE-ProRule annotationAdd
    BLAST
    Repeati354 – 36310GRSGPLPPXP motif 1
    Repeati393 – 40210GRSGPLPPXP motif 2
    Domaini448 – 46518WH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi162 – 1676Poly-Pro
    Compositional biasi314 – 3218Poly-Pro
    Compositional biasi324 – 34118Poly-GlyAdd
    BLAST
    Compositional biasi368 – 3736Poly-Pro
    Compositional biasi376 – 3794Poly-Pro
    Compositional biasi384 – 3907Poly-Pro
    Compositional biasi397 – 4037Poly-Pro
    Compositional biasi408 – 42417Poly-ProAdd
    BLAST
    Compositional biasi503 – 52018Asp/Glu-rich (acidic)Add
    BLAST

    Domaini

    The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
    The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270974.
    HOVERGENiHBG000222.
    InParanoidiP70315.
    KOiK05747.
    OMAiTPANEER.
    OrthoDBiEOG7FJH20.
    PhylomeDBiP70315.
    TreeFamiTF316736.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.90.810.10. 2 hits.
    InterProiIPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR027641. WASP.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view]
    PANTHERiPTHR12779:SF2. PTHR12779:SF2. 1 hit.
    PfamiPF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 1 hit.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47912. SSF47912. 2 hits.
    PROSITEiPS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT    50
    TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE 100
    QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR 150
    NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV 200
    TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS 250
    HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG 300
    GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG 350
    SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP 400
    PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG 450
    ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH 500
    SSDEGEDQTG EDEEDDEWDD 520
    Length:520
    Mass (Da):54,192
    Last modified:February 1, 1997 - v1
    Checksum:i9C223733C59F0C8A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54788 mRNA. Translation: AAC52556.1.
    CCDSiCCDS29984.1.
    RefSeqiNP_033541.1. NM_009515.2.
    UniGeneiMm.4735.

    Genome annotation databases

    EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165.
    GeneIDi22376.
    KEGGimmu:22376.
    UCSCiuc009sns.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54788 mRNA. Translation: AAC52556.1 .
    CCDSi CCDS29984.1.
    RefSeqi NP_033541.1. NM_009515.2.
    UniGenei Mm.4735.

    3D structure databases

    ProteinModelPortali P70315.
    SMRi P70315. Positions 37-159, 244-311, 482-510.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204544. 7 interactions.
    DIPi DIP-36426N.
    IntActi P70315. 12 interactions.
    MINTi MINT-1532664.

    PTM databases

    PhosphoSitei P70315.

    Proteomic databases

    PaxDbi P70315.
    PRIDEi P70315.

    Protocols and materials databases

    DNASUi 22376.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033505 ; ENSMUSP00000033505 ; ENSMUSG00000031165 .
    GeneIDi 22376.
    KEGGi mmu:22376.
    UCSCi uc009sns.2. mouse.

    Organism-specific databases

    CTDi 7454.
    MGIi MGI:105059. Was.

    Phylogenomic databases

    eggNOGi NOG270974.
    HOVERGENi HBG000222.
    InParanoidi P70315.
    KOi K05747.
    OMAi TPANEER.
    OrthoDBi EOG7FJH20.
    PhylomeDBi P70315.
    TreeFami TF316736.

    Enzyme and pathway databases

    Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    NextBioi 302721.
    PROi P70315.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70315.
    Bgeei P70315.
    CleanExi MM_WAS.
    Genevestigatori P70315.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.90.810.10. 2 hits.
    InterProi IPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR027641. WASP.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view ]
    PANTHERi PTHR12779:SF2. PTHR12779:SF2. 1 hit.
    Pfami PF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 1 hit.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47912. SSF47912. 2 hits.
    PROSITEi PS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP) gene is highly conserved and maps near the scurfy (sf) mutation on the X chromosome."
      Derry J.M.J., Wiedemann P., Blair P., Wang Y., Kerns J.A., Lemahieu V., Godfrey V.L., Wilkinson J.E., Francke U.
      Genomics 29:471-477(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling."
      Quilliam L.A., Lambert Q.T., Mickelson-Young L.A., Westwick J.K., Sparks A.B., Kay B.K., Jenkins N.A., Gilbert D.J., Copeland N.G., Der C.J.
      J. Biol. Chem. 271:28772-28776(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NCK1.
    3. "Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein."
      Wu Y., Spencer S.D., Lasky L.A.
      J. Biol. Chem. 273:5765-5770(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PSTPIP1.
    4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-293; SER-501 AND SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiWASP_MOUSE
    AccessioniPrimary (citable) accession number: P70315
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3