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Protein

Wiskott-Aldrich syndrome protein homolog

Gene

Was

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.

GO - Molecular functioni

GO - Biological processi

  • actin filament-based movement Source: MGI
  • actin filament polymerization Source: MGI
  • actin polymerization or depolymerization Source: MGI
  • endosomal transport Source: MGI
  • Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  • immune response Source: MGI
  • negative regulation of cell motility Source: MGI
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  • regulation of T cell antigen processing and presentation Source: MGI
  • T cell activation Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein homolog
Short name:
WASp
Gene namesi
Name:Was
Synonyms:Wasp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:105059. Was.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889911 – 520Wiskott-Aldrich syndrome protein homologAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei293PhosphotyrosineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70315.
PaxDbiP70315.
PeptideAtlasiP70315.
PRIDEiP70315.

PTM databases

iPTMnetiP70315.
PhosphoSitePlusiP70315.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031165.
CleanExiMM_WAS.
ExpressionAtlasiP70315. baseline and differential.
GenevisibleiP70315. MM.

Interactioni

Subunit structurei

Interacts with CDC42, RAC, NCK, FYN, HCK, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA (By similarity). Interacts with NCK1 (via SH3 domains).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ipo5Q8BKC58EBI-644195,EBI-643605
SrcP054802EBI-644195,EBI-298680
Wipf1Q8K1I73EBI-644195,EBI-644216

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204544. 6 interactors.
DIPiDIP-36426N.
IntActiP70315. 12 interactors.
MINTiMINT-1532664.
STRINGi10090.ENSMUSP00000033505.

Structurei

3D structure databases

ProteinModelPortaliP70315.
SMRiP70315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 150WH1PROSITE-ProRule annotationAdd BLAST110
Domaini240 – 253CRIBPROSITE-ProRule annotationAdd BLAST14
Repeati354 – 363GRSGPLPPXP motif 110
Repeati393 – 402GRSGPLPPXP motif 210
Domaini448 – 465WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 167Poly-Pro6
Compositional biasi314 – 321Poly-Pro8
Compositional biasi324 – 341Poly-GlyAdd BLAST18
Compositional biasi368 – 373Poly-Pro6
Compositional biasi376 – 379Poly-Pro4
Compositional biasi384 – 390Poly-Pro7
Compositional biasi397 – 403Poly-Pro7
Compositional biasi408 – 424Poly-ProAdd BLAST17
Compositional biasi503 – 520Asp/Glu-rich (acidic)Add BLAST18

Domaini

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111M1J. LUCA.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiP70315.
KOiK05747.
OMAiGPGVQQN.
OrthoDBiEOG091G0VCD.
PhylomeDBiP70315.
TreeFamiTF316736.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 2 hits.
InterProiIPR000095. CRIB_dom.
IPR011993. PH_dom-like.
IPR027641. WASP.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF35. PTHR23202:SF35. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT
60 70 80 90 100
TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE
110 120 130 140 150
QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR
160 170 180 190 200
NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV
210 220 230 240 250
TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS
260 270 280 290 300
HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG
310 320 330 340 350
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG
360 370 380 390 400
SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP
410 420 430 440 450
PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG
460 470 480 490 500
ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH
510 520
SSDEGEDQTG EDEEDDEWDD
Length:520
Mass (Da):54,192
Last modified:February 1, 1997 - v1
Checksum:i9C223733C59F0C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA. Translation: AAC52556.1.
CCDSiCCDS29984.1.
RefSeqiNP_033541.1. NM_009515.2.
UniGeneiMm.4735.

Genome annotation databases

EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165.
GeneIDi22376.
KEGGimmu:22376.
UCSCiuc009sns.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA. Translation: AAC52556.1.
CCDSiCCDS29984.1.
RefSeqiNP_033541.1. NM_009515.2.
UniGeneiMm.4735.

3D structure databases

ProteinModelPortaliP70315.
SMRiP70315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204544. 6 interactors.
DIPiDIP-36426N.
IntActiP70315. 12 interactors.
MINTiMINT-1532664.
STRINGi10090.ENSMUSP00000033505.

PTM databases

iPTMnetiP70315.
PhosphoSitePlusiP70315.

Proteomic databases

EPDiP70315.
PaxDbiP70315.
PeptideAtlasiP70315.
PRIDEiP70315.

Protocols and materials databases

DNASUi22376.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165.
GeneIDi22376.
KEGGimmu:22376.
UCSCiuc009sns.2. mouse.

Organism-specific databases

CTDi7454.
MGIiMGI:105059. Was.

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111M1J. LUCA.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiP70315.
KOiK05747.
OMAiGPGVQQN.
OrthoDBiEOG091G0VCD.
PhylomeDBiP70315.
TreeFamiTF316736.

Enzyme and pathway databases

ReactomeiR-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

PROiP70315.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031165.
CleanExiMM_WAS.
ExpressionAtlasiP70315. baseline and differential.
GenevisibleiP70315. MM.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 2 hits.
InterProiIPR000095. CRIB_dom.
IPR011993. PH_dom-like.
IPR027641. WASP.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF35. PTHR23202:SF35. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASP_MOUSE
AccessioniPrimary (citable) accession number: P70315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.