ID   PITX1_MOUSE             Reviewed;         315 AA.
AC   P70314; O54742; P70427;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   24-JAN-2024, entry version 190.
DE   RecName: Full=Pituitary homeobox 1;
DE   AltName: Full=Hindlimb-expressed homeobox protein backfoot;
DE   AltName: Full=Homeobox protein P-OTX;
DE   AltName: Full=Homeobox protein PITX1;
DE   AltName: Full=Paired-like homeodomain transcription factor 1;
DE   AltName: Full=Pituitary OTX-related factor;
GN   Name=Pitx1; Synonyms=Bft, Potx, Ptx1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=8755540; DOI=10.1073/pnas.93.15.7706;
RA   Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.;
RT   "P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior
RT   pituitary gland development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=8675014; DOI=10.1101/gad.10.10.1284;
RA   Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y.,
RA   Drouin J.;
RT   "Ptx1, a bicoid-related homeo box transcription factor involved in
RT   transcription of the pro-opiomelanocortin gene.";
RL   Genes Dev. 10:1284-1295(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Salivary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
RX   PubMed=9070926; DOI=10.1006/geno.1996.4558;
RA   Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.;
RT   "Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and
RT   mouse chromosome 13 (Bft).";
RL   Genomics 40:108-113(1997).
RN   [5]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=9226452; DOI=10.1242/dev.124.14.2807;
RA   Lanctot C., Lamolet B., Drouin J.;
RT   "The bicoid-related homeoprotein Ptx1 defines the most anterior domain of
RT   the embryo and differentiates posterior from anterior lateral mesoderm.";
RL   Development 124:2807-2817(1997).
CC   -!- FUNCTION: Sequence-specific transcription factor that binds gene
CC       promoters and activates their transcription. May play a role in the
CC       development of anterior structures, and in particular, the brain and
CC       facies and in specifying the identity or structure of hindlimb. Can
CC       independently activate and synergize with PIT-1 on pituitary-specific
CC       target gene promoters, thus may subserve functions in generating both
CC       precursor and specific cell phenotypes in the anterior pituitary gland
CC       and in several other organs. Can activate pituitary transcription of
CC       the proopiomelanocortin gene. {ECO:0000250|UniProtKB:P56673,
CC       ECO:0000269|PubMed:8675014, ECO:0000269|PubMed:8755540}.
CC   -!- SUBUNIT: Interacts with POU1F1. {ECO:0000250|UniProtKB:P78337}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in primordial Rathke pouch, oral
CC       epithelium, first branchial arch, duodenum, and hindlimb.
CC   -!- DEVELOPMENTAL STAGE: Expressed throughout pituitary development. Not
CC       found in 8 dpc embryos but seen in embryos at 8.5 dpc in the region of
CC       the first branchial arch and ventral portion of the caudal-most region
CC       of the embryo. At 9.5 dpc observed in the hindlimb buds, maxillary and
CC       mandibular components of the first branchial arch and Rathke pouch and
CC       the pattern of expression continues through 10.5 dpc at 11.5 dpc also
CC       found in ventral mesenchyme covering the abdominal cavity and in the
CC       nasal epithelium. At 17.5 dpc expressed in derivatives of the first
CC       branchial arch, including tongue and mandible as well as duodenum,
CC       salivary glands, nasal epithelium and condensing cartilage in the
CC       hindlimbs. {ECO:0000269|PubMed:9226452}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U54499; AAC52716.1; -; mRNA.
DR   EMBL; U71206; AAB16860.1; -; mRNA.
DR   EMBL; BC012696; AAH12696.1; -; mRNA.
DR   EMBL; U70371; AAC53059.1; -; mRNA.
DR   CCDS; CCDS26556.1; -.
DR   RefSeq; NP_035227.1; NM_011097.2.
DR   RefSeq; XP_006517220.1; XM_006517157.3.
DR   RefSeq; XP_006517221.1; XM_006517158.3.
DR   AlphaFoldDB; P70314; -.
DR   SMR; P70314; -.
DR   BioGRID; 202186; 4.
DR   STRING; 10090.ENSMUSP00000021968; -.
DR   PhosphoSitePlus; P70314; -.
DR   PaxDb; 10090-ENSMUSP00000021968; -.
DR   ProteomicsDB; 288174; -.
DR   Antibodypedia; 14722; 257 antibodies from 33 providers.
DR   DNASU; 18740; -.
DR   Ensembl; ENSMUST00000021968.7; ENSMUSP00000021968.6; ENSMUSG00000021506.8.
DR   GeneID; 18740; -.
DR   KEGG; mmu:18740; -.
DR   UCSC; uc007qsd.1; mouse.
DR   AGR; MGI:107374; -.
DR   CTD; 5307; -.
DR   MGI; MGI:107374; Pitx1.
DR   VEuPathDB; HostDB:ENSMUSG00000021506; -.
DR   eggNOG; KOG0486; Eukaryota.
DR   GeneTree; ENSGT00940000154518; -.
DR   HOGENOM; CLU_030301_0_0_1; -.
DR   InParanoid; P70314; -.
DR   OMA; WHAKSLA; -.
DR   OrthoDB; 5395268at2759; -.
DR   PhylomeDB; P70314; -.
DR   TreeFam; TF351940; -.
DR   BioGRID-ORCS; 18740; 3 hits in 79 CRISPR screens.
DR   ChiTaRS; Pitx1; mouse.
DR   PRO; PR:P70314; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; P70314; Protein.
DR   Bgee; ENSMUSG00000021506; Expressed in mouth mucosa and 151 other cell types or tissues.
DR   ExpressionAtlas; P70314; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR   GO; GO:0014707; P:branchiomeric skeletal muscle development; IMP:MGI.
DR   GO; GO:0051216; P:cartilage development; IMP:MGI.
DR   GO; GO:0035116; P:embryonic hindlimb morphogenesis; IMP:CACAO.
DR   GO; GO:0035137; P:hindlimb morphogenesis; IDA:MGI.
DR   GO; GO:0048625; P:myoblast fate commitment; IMP:MGI.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:CACAO.
DR   GO; GO:0021983; P:pituitary gland development; IMP:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; ISO:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR016233; Homeobox_Pitx/unc30.
DR   InterPro; IPR003654; OAR_dom.
DR   PANTHER; PTHR45882:SF1; PITUITARY HOMEOBOX 1; 1.
DR   PANTHER; PTHR45882; PITUITARY HOMEOBOX HOMOLOG PTX1; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF03826; OAR; 1.
DR   PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS50803; OAR; 1.
DR   Genevisible; P70314; MM.
PE   2: Evidence at transcript level;
KW   Acetylation; Activator; Developmental protein; DNA-binding; Homeobox;
KW   Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..315
FT                   /note="Pituitary homeobox 1"
FT                   /id="PRO_0000049219"
FT   DNA_BIND        90..149
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          151..280
FT                   /note="Interaction with PIT-1"
FT   MOTIF           281..294
FT                   /note="OAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT   MOTIF           287..291
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        45..66
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P78337"
FT   MOD_RES         46
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P78337"
FT   MOD_RES         48
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P78337"
FT   CONFLICT        287
FT                   /note="R -> A (in Ref. 1; AAC52716)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   315 AA;  34075 MW;  6E1C24718DCA3F30 CRC64;
     MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES SDADLPDKER
     GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ QLQELEATFQ RNRYPDMSMR
     EEIAVWTNLT EPRVRVWFKN RRAKWRKRER NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY
     SYNNWAAKSL APAPLSTKSF TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN
     SGLNNINNLT GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG
     LQGPASGLNA CQYNS
//