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Protein

Pituitary homeobox 1

Gene

Pitx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Acts as a transcriptional activator by binding to promoter sequences with a TAAT/GCC core motif. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi90 – 14960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • branchiomeric skeletal muscle development Source: MGI
  • cartilage development Source: MGI
  • embryonic hindlimb morphogenesis Source: CACAO
  • hindlimb morphogenesis Source: MGI
  • myoblast fate commitment Source: MGI
  • negative regulation of transcription, DNA-templated Source: CACAO
  • pituitary gland development Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • skeletal system development Source: MGI
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 1
Alternative name(s):
Hindlimb-expressed homeobox protein backfoot
Homeobox protein P-OTX
Homeobox protein PITX1
Paired-like homeodomain transcription factor 1
Pituitary OTX-related factor
Gene namesi
Name:Pitx1
Synonyms:Bft, Potx, Ptx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:107374. Pitx1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Pituitary homeobox 1PRO_0000049219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP70314.

PTM databases

PhosphoSiteiP70314.

Expressioni

Tissue specificityi

Expressed in primordial Rathke pouch, oral epithelium, first branchial arch, duodenum, and hindlimb.

Developmental stagei

Expressed throughout pituitary development. Not found in E8 embryos but seen in embryos at E8.5 in the region of the first branchial arch and ventral portion of the caudal-most region of the embryo. At E9.5 observed in the hindlimb buds, maxillary and mandibular components of the first branchial arch and Rathke pouch and the pattern of expression continues through E10.5 at E11.5 also found in ventral mesenchyme covering the abdominal cavity and in the nasal epithelium. At E17.5 expressed in derivatives of the first branchial arch, including tongue and mandible as well as duodenum, salivary glands, nasal epithelium and condensing cartilage in the hindlimbs.1 Publication

Gene expression databases

BgeeiP70314.
CleanExiMM_PITX1.
ExpressionAtlasiP70314. baseline and differential.
GenevisibleiP70314. MM.

Interactioni

Protein-protein interaction databases

BioGridi202186. 4 interactions.
STRINGi10090.ENSMUSP00000021968.

Structurei

3D structure databases

ProteinModelPortaliP70314.
SMRiP70314. Positions 90-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 280130Interacts with PIT-1Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi281 – 29414OARPROSITE-ProRule annotationAdd
BLAST
Motifi287 – 2915Nuclear localization signalSequence Analysis

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG249113.
GeneTreeiENSGT00760000118908.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiP70314.
KOiK09356.
OMAiCKNSYLP.
OrthoDBiEOG7VTDNW.
PhylomeDBiP70314.
TreeFamiTF351940.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES
60 70 80 90 100
SDADLPDKER GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ
110 120 130 140 150
QLQELEATFQ RNRYPDMSMR EEIAVWTNLT EPRVRVWFKN RRAKWRKRER
160 170 180 190 200
NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY SYNNWAAKSL APAPLSTKSF
210 220 230 240 250
TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN SGLNNINNLT
260 270 280 290 300
GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG
310
LQGPASGLNA CQYNS
Length:315
Mass (Da):34,075
Last modified:July 15, 1999 - v2
Checksum:i6E1C24718DCA3F30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti287 – 2871R → A in AAC52716 (PubMed:8755540).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54499 mRNA. Translation: AAC52716.1.
U71206 mRNA. Translation: AAB16860.1.
BC012696 mRNA. Translation: AAH12696.1.
U70371 mRNA. Translation: AAC53059.1.
CCDSiCCDS26556.1.
RefSeqiNP_035227.1. NM_011097.2.
XP_006517220.1. XM_006517157.2.
XP_006517221.1. XM_006517158.2.
UniGeneiMm.135195.

Genome annotation databases

EnsembliENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
GeneIDi18740.
KEGGimmu:18740.
UCSCiuc007qsd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54499 mRNA. Translation: AAC52716.1.
U71206 mRNA. Translation: AAB16860.1.
BC012696 mRNA. Translation: AAH12696.1.
U70371 mRNA. Translation: AAC53059.1.
CCDSiCCDS26556.1.
RefSeqiNP_035227.1. NM_011097.2.
XP_006517220.1. XM_006517157.2.
XP_006517221.1. XM_006517158.2.
UniGeneiMm.135195.

3D structure databases

ProteinModelPortaliP70314.
SMRiP70314. Positions 90-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202186. 4 interactions.
STRINGi10090.ENSMUSP00000021968.

PTM databases

PhosphoSiteiP70314.

Proteomic databases

PRIDEiP70314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
GeneIDi18740.
KEGGimmu:18740.
UCSCiuc007qsd.1. mouse.

Organism-specific databases

CTDi5307.
MGIiMGI:107374. Pitx1.

Phylogenomic databases

eggNOGiNOG249113.
GeneTreeiENSGT00760000118908.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiP70314.
KOiK09356.
OMAiCKNSYLP.
OrthoDBiEOG7VTDNW.
PhylomeDBiP70314.
TreeFamiTF351940.

Miscellaneous databases

NextBioi294881.
PROiP70314.
SOURCEiSearch...

Gene expression databases

BgeeiP70314.
CleanExiMM_PITX1.
ExpressionAtlasiP70314. baseline and differential.
GenevisibleiP70314. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior pituitary gland development."
    Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.
    Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene."
    Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y., Drouin J.
    Genes Dev. 10:1284-1295(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and mouse chromosome 13 (Bft)."
    Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.
    Genomics 40:108-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
  5. "The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm."
    Lanctot C., Lamolet B., Drouin J.
    Development 124:2807-2817(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPITX1_MOUSE
AccessioniPrimary (citable) accession number: P70314
Secondary accession number(s): O54742, P70427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: July 22, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.