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P70314 (PITX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary homeobox 1
Alternative name(s):
Hindlimb-expressed homeobox protein backfoot
Homeobox protein P-OTX
Homeobox protein PITX1
Paired-like homeodomain transcription factor 1
Pituitary OTX-related factor
Gene names
Name:Pitx1
Synonyms:Bft, Potx, Ptx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Acts as a transcriptional activator by binding to promoter sequences with a TAAT/GCC core motif. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.

Subcellular location

Nucleus.

Tissue specificity

Expressed in primordial Rathke pouch, oral epithelium, first branchial arch, duodenum, and hindlimb.

Developmental stage

Expressed throughout pituitary development. Not found in E8 embryos but seen in embryos at E8.5 in the region of the first branchial arch and ventral portion of the caudal-most region of the embryo. At E9.5 observed in the hindlimb buds, maxillary and mandibular components of the first branchial arch and Rathke pouch and the pattern of expression continues through E10.5 at E11.5 also found in ventral mesenchyme covering the abdominal cavity and in the nasal epithelium. At E17.5 expressed in derivatives of the first branchial arch, including tongue and mandible as well as duodenum, salivary glands, nasal epithelium and condensing cartilage in the hindlimbs. Ref.5

Sequence similarities

Belongs to the paired homeobox family. Bicoid subfamily.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbranchiomeric skeletal muscle development

Inferred from mutant phenotype PubMed 17384148. Source: MGI

cartilage development

Inferred from mutant phenotype PubMed 10101115. Source: MGI

embryonic hindlimb morphogenesis

Inferred from genetic interaction PubMed 18231602. Source: MGI

hindlimb morphogenesis

Inferred from direct assay PubMed 15621531PubMed 16989801. Source: MGI

myoblast fate commitment

Inferred from mutant phenotype PubMed 17384148. Source: MGI

pituitary gland development

Inferred from mutant phenotype PubMed 10049363. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 9192866. Source: MGI

skeletal system development

Inferred from mutant phenotype PubMed 10101115. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 19531352. Source: MGI

nucleolus

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 19531352. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 9207461. Source: MGI

   Molecular_functionRNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription

Inferred from physical interaction PubMed 9192866. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Pituitary homeobox 1
PRO_0000049219

Regions

DNA binding90 – 14960Homeobox
Region151 – 280130Interacts with PIT-1
Motif281 – 29414OAR
Motif287 – 2915Nuclear localization signal Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue461Phosphoserine By similarity
Modified residue481Phosphoserine By similarity

Experimental info

Sequence conflict2871R → A in AAC52716. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P70314 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 6E1C24718DCA3F30

FASTA31534,075
        10         20         30         40         50         60 
MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES SDADLPDKER 

        70         80         90        100        110        120 
GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ QLQELEATFQ RNRYPDMSMR 

       130        140        150        160        170        180 
EEIAVWTNLT EPRVRVWFKN RRAKWRKRER NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY 

       190        200        210        220        230        240 
SYNNWAAKSL APAPLSTKSF TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN 

       250        260        270        280        290        300 
SGLNNINNLT GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG 

       310 
LQGPASGLNA CQYNS 

« Hide

References

« Hide 'large scale' references
[1]"P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior pituitary gland development."
Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.
Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]"Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene."
Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y., Drouin J.
Genes Dev. 10:1284-1295(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[4]"Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and mouse chromosome 13 (Bft)."
Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.
Genomics 40:108-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
[5]"The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm."
Lanctot C., Lamolet B., Drouin J.
Development 124:2807-2817(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U54499 mRNA. Translation: AAC52716.1.
U71206 mRNA. Translation: AAB16860.1.
BC012696 mRNA. Translation: AAH12696.1.
U70371 mRNA. Translation: AAC53059.1.
CCDSCCDS26556.1.
RefSeqNP_035227.1. NM_011097.2.
XP_006517220.1. XM_006517157.1.
XP_006517221.1. XM_006517158.1.
UniGeneMm.135195.

3D structure databases

ProteinModelPortalP70314.
SMRP70314. Positions 90-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202186. 4 interactions.

PTM databases

PhosphoSiteP70314.

Proteomic databases

PRIDEP70314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
GeneID18740.
KEGGmmu:18740.
UCSCuc007qsd.1. mouse.

Organism-specific databases

CTD5307.
MGIMGI:107374. Pitx1.

Phylogenomic databases

eggNOGNOG249113.
HOGENOMHOG000273886.
HOVERGENHBG068770.
InParanoidP70314.
KOK09356.
OMACKNSYLP.
OrthoDBEOG7VTDNW.
PhylomeDBP70314.
TreeFamTF351940.

Gene expression databases

BgeeP70314.
CleanExMM_PITX1.
GenevestigatorP70314.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294881.
PROP70314.
SOURCESearch...

Entry information

Entry namePITX1_MOUSE
AccessionPrimary (citable) accession number: P70314
Secondary accession number(s): O54742, P70427
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot