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P70314

- PITX1_MOUSE

UniProt

P70314 - PITX1_MOUSE

Protein

Pituitary homeobox 1

Gene

Pitx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Acts as a transcriptional activator by binding to promoter sequences with a TAAT/GCC core motif. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi90 – 14960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    3. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI

    GO - Biological processi

    1. branchiomeric skeletal muscle development Source: MGI
    2. cartilage development Source: MGI
    3. embryonic hindlimb morphogenesis Source: MGI
    4. hindlimb morphogenesis Source: MGI
    5. myoblast fate commitment Source: MGI
    6. pituitary gland development Source: MGI
    7. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    8. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pituitary homeobox 1
    Alternative name(s):
    Hindlimb-expressed homeobox protein backfoot
    Homeobox protein P-OTX
    Homeobox protein PITX1
    Paired-like homeodomain transcription factor 1
    Pituitary OTX-related factor
    Gene namesi
    Name:Pitx1
    Synonyms:Bft, Potx, Ptx1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:107374. Pitx1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleolus Source: Ensembl
    3. nucleus Source: MGI
    4. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Pituitary homeobox 1PRO_0000049219Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei46 – 461PhosphoserineBy similarity
    Modified residuei48 – 481PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP70314.

    PTM databases

    PhosphoSiteiP70314.

    Expressioni

    Tissue specificityi

    Expressed in primordial Rathke pouch, oral epithelium, first branchial arch, duodenum, and hindlimb.

    Developmental stagei

    Expressed throughout pituitary development. Not found in E8 embryos but seen in embryos at E8.5 in the region of the first branchial arch and ventral portion of the caudal-most region of the embryo. At E9.5 observed in the hindlimb buds, maxillary and mandibular components of the first branchial arch and Rathke pouch and the pattern of expression continues through E10.5 at E11.5 also found in ventral mesenchyme covering the abdominal cavity and in the nasal epithelium. At E17.5 expressed in derivatives of the first branchial arch, including tongue and mandible as well as duodenum, salivary glands, nasal epithelium and condensing cartilage in the hindlimbs.1 Publication

    Gene expression databases

    BgeeiP70314.
    CleanExiMM_PITX1.
    GenevestigatoriP70314.

    Interactioni

    Protein-protein interaction databases

    BioGridi202186. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP70314.
    SMRiP70314. Positions 90-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni151 – 280130Interacts with PIT-1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi281 – 29414OARAdd
    BLAST
    Motifi287 – 2915Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG249113.
    HOGENOMiHOG000273886.
    HOVERGENiHBG068770.
    InParanoidiP70314.
    KOiK09356.
    OMAiCKNSYLP.
    OrthoDBiEOG7VTDNW.
    PhylomeDBiP70314.
    TreeFamiTF351940.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR016233. Homeobox_Pitx/unc30.
    IPR009057. Homeodomain-like.
    IPR003654. OAR_dom.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF03826. OAR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS50803. OAR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70314-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES    50
    SDADLPDKER GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ 100
    QLQELEATFQ RNRYPDMSMR EEIAVWTNLT EPRVRVWFKN RRAKWRKRER 150
    NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY SYNNWAAKSL APAPLSTKSF 200
    TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN SGLNNINNLT 250
    GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG 300
    LQGPASGLNA CQYNS 315
    Length:315
    Mass (Da):34,075
    Last modified:July 15, 1999 - v2
    Checksum:i6E1C24718DCA3F30
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti287 – 2871R → A in AAC52716. (PubMed:8755540)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54499 mRNA. Translation: AAC52716.1.
    U71206 mRNA. Translation: AAB16860.1.
    BC012696 mRNA. Translation: AAH12696.1.
    U70371 mRNA. Translation: AAC53059.1.
    CCDSiCCDS26556.1.
    RefSeqiNP_035227.1. NM_011097.2.
    XP_006517220.1. XM_006517157.1.
    XP_006517221.1. XM_006517158.1.
    UniGeneiMm.135195.

    Genome annotation databases

    EnsembliENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
    GeneIDi18740.
    KEGGimmu:18740.
    UCSCiuc007qsd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54499 mRNA. Translation: AAC52716.1 .
    U71206 mRNA. Translation: AAB16860.1 .
    BC012696 mRNA. Translation: AAH12696.1 .
    U70371 mRNA. Translation: AAC53059.1 .
    CCDSi CCDS26556.1.
    RefSeqi NP_035227.1. NM_011097.2.
    XP_006517220.1. XM_006517157.1.
    XP_006517221.1. XM_006517158.1.
    UniGenei Mm.135195.

    3D structure databases

    ProteinModelPortali P70314.
    SMRi P70314. Positions 90-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202186. 4 interactions.

    PTM databases

    PhosphoSitei P70314.

    Proteomic databases

    PRIDEi P70314.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021968 ; ENSMUSP00000021968 ; ENSMUSG00000021506 .
    GeneIDi 18740.
    KEGGi mmu:18740.
    UCSCi uc007qsd.1. mouse.

    Organism-specific databases

    CTDi 5307.
    MGIi MGI:107374. Pitx1.

    Phylogenomic databases

    eggNOGi NOG249113.
    HOGENOMi HOG000273886.
    HOVERGENi HBG068770.
    InParanoidi P70314.
    KOi K09356.
    OMAi CKNSYLP.
    OrthoDBi EOG7VTDNW.
    PhylomeDBi P70314.
    TreeFami TF351940.

    Miscellaneous databases

    NextBioi 294881.
    PROi P70314.
    SOURCEi Search...

    Gene expression databases

    Bgeei P70314.
    CleanExi MM_PITX1.
    Genevestigatori P70314.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR016233. Homeobox_Pitx/unc30.
    IPR009057. Homeodomain-like.
    IPR003654. OAR_dom.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF03826. OAR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS50803. OAR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior pituitary gland development."
      Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.
      Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    2. "Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene."
      Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y., Drouin J.
      Genes Dev. 10:1284-1295(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    4. "Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and mouse chromosome 13 (Bft)."
      Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.
      Genomics 40:108-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
    5. "The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm."
      Lanctot C., Lamolet B., Drouin J.
      Development 124:2807-2817(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiPITX1_MOUSE
    AccessioniPrimary (citable) accession number: P70314
    Secondary accession number(s): O54742, P70427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3