Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70314

- PITX1_MOUSE

UniProt

P70314 - PITX1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Pituitary homeobox 1

Gene

Pitx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Acts as a transcriptional activator by binding to promoter sequences with a TAAT/GCC core motif. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi90 – 14960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  3. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI

GO - Biological processi

  1. branchiomeric skeletal muscle development Source: MGI
  2. cartilage development Source: MGI
  3. embryonic hindlimb morphogenesis Source: MGI
  4. hindlimb morphogenesis Source: MGI
  5. myoblast fate commitment Source: MGI
  6. pituitary gland development Source: MGI
  7. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  8. skeletal system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 1
Alternative name(s):
Hindlimb-expressed homeobox protein backfoot
Homeobox protein P-OTX
Homeobox protein PITX1
Paired-like homeodomain transcription factor 1
Pituitary OTX-related factor
Gene namesi
Name:Pitx1
Synonyms:Bft, Potx, Ptx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:107374. Pitx1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleolus Source: Ensembl
  3. nucleus Source: MGI
  4. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Pituitary homeobox 1PRO_0000049219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP70314.

PTM databases

PhosphoSiteiP70314.

Expressioni

Tissue specificityi

Expressed in primordial Rathke pouch, oral epithelium, first branchial arch, duodenum, and hindlimb.

Developmental stagei

Expressed throughout pituitary development. Not found in E8 embryos but seen in embryos at E8.5 in the region of the first branchial arch and ventral portion of the caudal-most region of the embryo. At E9.5 observed in the hindlimb buds, maxillary and mandibular components of the first branchial arch and Rathke pouch and the pattern of expression continues through E10.5 at E11.5 also found in ventral mesenchyme covering the abdominal cavity and in the nasal epithelium. At E17.5 expressed in derivatives of the first branchial arch, including tongue and mandible as well as duodenum, salivary glands, nasal epithelium and condensing cartilage in the hindlimbs.1 Publication

Gene expression databases

BgeeiP70314.
CleanExiMM_PITX1.
ExpressionAtlasiP70314. differential.
GenevestigatoriP70314.

Interactioni

Protein-protein interaction databases

BioGridi202186. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP70314.
SMRiP70314. Positions 90-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 280130Interacts with PIT-1Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi281 – 29414OARAdd
BLAST
Motifi287 – 2915Nuclear localization signalSequence Analysis

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG249113.
HOGENOMiHOG000273886.
HOVERGENiHBG068770.
InParanoidiP70314.
KOiK09356.
OMAiCKNSYLP.
OrthoDBiEOG7VTDNW.
PhylomeDBiP70314.
TreeFamiTF351940.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
PIRSFiPIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70314-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES
60 70 80 90 100
SDADLPDKER GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ
110 120 130 140 150
QLQELEATFQ RNRYPDMSMR EEIAVWTNLT EPRVRVWFKN RRAKWRKRER
160 170 180 190 200
NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY SYNNWAAKSL APAPLSTKSF
210 220 230 240 250
TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN SGLNNINNLT
260 270 280 290 300
GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG
310
LQGPASGLNA CQYNS
Length:315
Mass (Da):34,075
Last modified:July 15, 1999 - v2
Checksum:i6E1C24718DCA3F30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti287 – 2871R → A in AAC52716. (PubMed:8755540)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54499 mRNA. Translation: AAC52716.1.
U71206 mRNA. Translation: AAB16860.1.
BC012696 mRNA. Translation: AAH12696.1.
U70371 mRNA. Translation: AAC53059.1.
CCDSiCCDS26556.1.
RefSeqiNP_035227.1. NM_011097.2.
XP_006517220.1. XM_006517157.1.
XP_006517221.1. XM_006517158.1.
UniGeneiMm.135195.

Genome annotation databases

EnsembliENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
GeneIDi18740.
KEGGimmu:18740.
UCSCiuc007qsd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54499 mRNA. Translation: AAC52716.1 .
U71206 mRNA. Translation: AAB16860.1 .
BC012696 mRNA. Translation: AAH12696.1 .
U70371 mRNA. Translation: AAC53059.1 .
CCDSi CCDS26556.1.
RefSeqi NP_035227.1. NM_011097.2.
XP_006517220.1. XM_006517157.1.
XP_006517221.1. XM_006517158.1.
UniGenei Mm.135195.

3D structure databases

ProteinModelPortali P70314.
SMRi P70314. Positions 90-149.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202186. 4 interactions.

PTM databases

PhosphoSitei P70314.

Proteomic databases

PRIDEi P70314.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021968 ; ENSMUSP00000021968 ; ENSMUSG00000021506 .
GeneIDi 18740.
KEGGi mmu:18740.
UCSCi uc007qsd.1. mouse.

Organism-specific databases

CTDi 5307.
MGIi MGI:107374. Pitx1.

Phylogenomic databases

eggNOGi NOG249113.
HOGENOMi HOG000273886.
HOVERGENi HBG068770.
InParanoidi P70314.
KOi K09356.
OMAi CKNSYLP.
OrthoDBi EOG7VTDNW.
PhylomeDBi P70314.
TreeFami TF351940.

Miscellaneous databases

NextBioi 294881.
PROi P70314.
SOURCEi Search...

Gene expression databases

Bgeei P70314.
CleanExi MM_PITX1.
ExpressionAtlasi P70314. differential.
Genevestigatori P70314.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR016233. Homeobox_Pitx/unc30.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view ]
PIRSFi PIRSF000563. Homeobox_protein_Pitx/Unc30. 1 hit.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior pituitary gland development."
    Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.
    Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene."
    Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y., Drouin J.
    Genes Dev. 10:1284-1295(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and mouse chromosome 13 (Bft)."
    Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.
    Genomics 40:108-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
  5. "The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm."
    Lanctot C., Lamolet B., Drouin J.
    Development 124:2807-2817(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPITX1_MOUSE
AccessioniPrimary (citable) accession number: P70314
Secondary accession number(s): O54742, P70427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3