Hyaluronan synthase 2 - Mus musculus (Mouse)

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P70312 (HAS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hyaluronan synthase 2
EC=2.4.1.212

Alternative name(s):
Hyaluronate synthase 2
Hyaluronic acid synthase 2
Short name=HA synthase 2

Gene names

Name:

Has2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in heart, brain, spleen, lung and skeletal muscle.
Developmental stage	Expressed at day 7.5.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 552

552

Hyaluronan synthase 2

PRO_0000197174

Regions

Topological domain

1 – 11

Cytoplasmic Potential

Transmembrane

12 – 32

Helical; Name=1; Potential

Topological domain

33 – 45

Extracellular Potential

Transmembrane

46 – 66

Helical; Name=2; Potential

Topological domain

67 – 374

308

Cytoplasmic Potential

Transmembrane

375 – 395

Helical; Name=3; Potential

Topological domain

396 – 402

Extracellular Potential

Transmembrane

403 – 423

Helical; Name=4; Potential

Topological domain

424 – 429

Cytoplasmic Potential

Transmembrane

430 – 450

Helical; Name=5; Potential

Topological domain

451 – 475

Extracellular Potential

Transmembrane

476 – 496

Helical; Name=6; Potential

Topological domain

497 – 510

Cytoplasmic Potential

Transmembrane

511 – 531

Helical; Name=7; Potential

Topological domain

532 – 552

Extracellular Potential

Experimental info

Sequence conflict

138

M → I in AAC53309. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P70312 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: B840BABC6D2260D5
Show »

FASTA

552

63,510

        10         20         30         40         50         60 
MHCERFLCVL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG AFLASHLIIQ 

        70         80         90        100        110        120 
SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR KCLQSVKRLT YPGIKVVMVI 

       130        140        150        160        170        180 
DGNSDDDLYM MDIFSEVMGR DKSATYIWKN NFHEKGPGET EESHKESSQH VTQLVLSNKS 

       190        200        210        220        230        240 
ICIMQKWGGK REVMYTAFRA LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG 

       250        260        270        280        290        300 
DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 

       310        320        330        340        350        360 
NQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ QTRWSKSYFR 

       370        380        390        400        410        420 
EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GKIWNILLFL LTVQLVGLIK 

       430        440        450        460        470        480 
SSFASCLRGN IVMVFMSLYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 

       490        500        510        520        530        540 
VSVWFTILLG GVIFTIYKES KKPFSESKQT VLIVGTLIYA CYWVMLLTLY VVLINKCGRR 

       550 
KKGQQYDMVL DV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a putative mouse hyaluronan synthase." Spicer A.P., Augustine M.L., McDonald J.A. J. Biol. Chem. 271:23400-23406(1996) [PubMed: 8798545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	Spicer A.P., Augustine M.L., McDonald J.A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 138.
[3]	"Coding sequence of a hyaluronan synthase homologue expressed during expansion of the mouse cumulus-oocyte complex." Fueloep C., Salustri A., Hascall V.C. Arch. Biochem. Biophys. 337:261-266(1997) [PubMed: 9016821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss CD1.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[7]	"Homologs of the Xenopus developmental gene DG42 are present in zebrafish and mouse and are involved in the synthesis of Nod-like chitin oligosaccharides during early embryogenesis." Semino C.E., Specht C.A., Raimondi A., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 93:4548-4553(1996) [PubMed: 8643441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 215-348. Strain: Swiss Webster. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52524 mRNA. Translation: AAC53309.2. U69695 mRNA. Translation: AAB17609.1. AK079729 mRNA. Translation: BAC37733.1. CH466545 Genomic DNA. Translation: EDL29280.1. BC080281 mRNA. Translation: AAH80281.1. U53222 Genomic DNA. Translation: AAC52651.1.
IPI	IPI00108079.
RefSeq	NP_032242.3. NM_008216.3.
UniGene	Mm.5148.
3D structure databases
ProteinModelPortal	P70312.
ModBase	Search...
Protein-protein interaction databases
STRING	P70312.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
PTM databases
PhosphoSite	P70312.
Proteomic databases
PRIDE	P70312.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050544; ENSMUSP00000062212; ENSMUSG00000022367.
GeneID	15117.
KEGG	mmu:15117.
Organism-specific databases
CTD	3037.
MGI	MGI:107821. Has2.
Phylogenomic databases
eggNOG	roNOG05966.
HOGENOM	HBG716008.
HOVERGEN	HBG000189.
InParanoid	P70312.
OrthoDB	EOG4KH2TP.
Enzyme and pathway databases
BRENDA	2.4.1.212. 3474.
Gene expression databases
ArrayExpress	P70312.
Bgee	P70312.
CleanEx	MM_HAS2.
Genevestigator	P70312.
GermOnline	ENSMUSG00000022367. Mus musculus.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001173. Glyco_trans_2. [Graphical view]
KO	K00752.
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	287534.
SOURCE	Search...

Entry information

Entry name

HAS2_MOUSE

Accession

Primary (citable) accession number: P70312
Secondary accession number(s): P70411, Q62405, Q68EF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 83 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents