P70310 (NTR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurotensin receptor type 2
Short name=NT-R-2
Short name=NTR2

Alternative name(s):
Low-affinity levocabastine-sensitive neurotensin receptor
NTRL

Gene names

Name:

Ntsr2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed maximally in the cerebellum, hyppocampus, piriform cortex and neocortex of adult brain.
Developmental stage	Expressed poorly in 7-day-old brain. Expression increases at day 15 to reach a maximal level in 35-day-old brain.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR2 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay Ref.1. Source: MGI regulation of membrane potential Inferred from direct assay Ref.1. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay Ref.1. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled neurotensin receptor activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Neurotensin receptor type 2		PRO_0000069950
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 55	23	Helical; Name=1; Potential
	Topological domain	56 – 64	9	Cytoplasmic Potential
	Transmembrane	65 – 87	23	Helical; Name=2; Potential
	Topological domain	88 – 109	22	Extracellular Potential
	Transmembrane	110 – 131	22	Helical; Name=3; Potential
	Topological domain	132 – 154	23	Cytoplasmic Potential
	Transmembrane	155 – 176	22	Helical; Name=4; Potential
	Topological domain	177 – 217	41	Extracellular Potential
	Transmembrane	218 – 238	21	Helical; Name=5; Potential
	Topological domain	239 – 298	60	Cytoplasmic Potential
	Transmembrane	299 – 319	21	Helical; Name=6; Potential
	Topological domain	320 – 338	19	Extracellular Potential
	Transmembrane	339 – 359	21	Helical; Name=7; Potential
	Topological domain	360 – 417	58	Cytoplasmic Potential
Amino acid modifications
	Lipidation	378	1	S-palmitoyl cysteine Potential
	Disulfide bond	108 ↔ 194		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70310 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EBFDDBD6507223DD	FASTA	417	46,537
	10 20 30 40 50 60 METSSLWPPR PSPSAGLSLE ARLGVDTRLW AKVLFTALYS LIFALGTAGN ALSVHVVLKA 70 80 90 100 110 120 RTGRPGRLRY HVLSLALSAL LLLLISVPME LYNFVWSHYP WVFGDLGCRG YYFVRELCAY 130 140 150 160 170 180 ATVLSVASLS AERCLAVCQP LRARRLLTPR RTCRLLSLVW VASLGLALPM AVIMGQKHEM 190 200 210 220 230 240 ERADGEPEPA SRVCTVLVSR ASSRSTFQVK RAGLLRSPLW ELTAILNGIT VNHLVALYSQ 250 260 270 280 290 300 VPSASAQVNS IPSRLELLSE EGLLGFITWR KTLSLGVQAS LVRHKDASQI RSLQHSAQVL 310 320 330 340 350 360 RAIVAVYVIC WLPYHARRLM YCYIPDDGWT DELYDFYHYF YMVTNTLFYV SSAVTPVLYN 370 380 390 400 410 AVSSSFRKLF LESLSSLCGE QRSVVPLPQE APESTTSTYS FRLWGSPRNP SLGEIQV

References

[1]	"Structure, functional expression, and cerebral localization of the levocabastine-sensitive neurotensin/neuromedin N receptor from mouse brain." Mazella J., Botto J.-M., Guillemare E., Coppola T., Sarret P., Vincent J.-P. J. Neurosci. 16:5613-5620(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51908 mRNA. Translation: AAB17285.1.
IPI	IPI00346625.
UniGene	Mm.281715.
3D structure databases
ProteinModelPortal	P70310.
SMR	P70310. Positions 20-369.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P70310.
Proteomic databases
PRIDE	P70310.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:108018. Ntsr2.
Phylogenomic databases
eggNOG	NOG303702.
HOGENOM	HOG000231271.
HOVERGEN	HBG098285.
OrthoDB	EOG4V438D.
Gene expression databases
CleanEx	MM_NTSR2.
Genevestigator	P70310.
GermOnline	ENSMUSG00000020591. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR003986. NT2_rcpt. IPR003984. NT_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01479. NEUROTENSINR. PR01481. NEUROTENSN2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P70310.
ChEMBL	CHEMBL2580.
SOURCE	Search...

Entry information

Entry name

NTR2_MOUSE

Accession

Primary (citable) accession number: P70310

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program