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Protein

Stromal interaction molecule 1

Gene

Stim1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi76 – 87By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • activation of store-operated calcium channel activity Source: UniProtKB
  • cation transport Source: MGI
  • cellular calcium ion homeostasis Source: GO_Central
  • detection of calcium ion Source: UniProtKB
  • enamel mineralization Source: UniProtKB
  • myotube differentiation Source: MGI
  • regulation of calcium ion transport Source: UniProtKB
  • regulation of store-operated calcium entry Source: UniProtKB
  • store-operated calcium entry Source: MGI

Keywordsi

Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5578775. Ion homeostasis.
R-MMU-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 1
Gene namesi
Name:Stim1
Synonyms:Sim
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107476. Stim1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 213ExtracellularSequence analysisAdd BLAST191
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 685CytoplasmicSequence analysisAdd BLAST451

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane, Microtubule, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003332723 – 685Stromal interaction molecule 1Add BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi171N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei257PhosphoserineCombined sources1
Modified residuei504PhosphothreonineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei517PhosphothreonineBy similarity1
Modified residuei519PhosphoserineCombined sources1
Modified residuei521PhosphoserineCombined sources1
Modified residuei523PhosphoserineBy similarity1
Modified residuei524PhosphoserineCombined sources1
Modified residuei567PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei602PhosphoserineBy similarity1
Modified residuei608PhosphoserineBy similarity1
Modified residuei618PhosphoserineBy similarity1
Modified residuei621PhosphoserineBy similarity1
Modified residuei628PhosphoserineBy similarity1
Modified residuei660PhosphoserineCombined sources1
Modified residuei665PhosphothreonineCombined sources1
Modified residuei668PhosphoserineCombined sources1

Post-translational modificationi

Glycosylation is required for cell surface expression.By similarity
Phosphorylated predominantly on Ser residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP70302.
MaxQBiP70302.
PaxDbiP70302.
PeptideAtlasiP70302.
PRIDEiP70302.

PTM databases

iPTMnetiP70302.
PhosphoSitePlusiP70302.

Expressioni

Tissue specificityi

Expressed in maturation-stage ameloblasts (at protein level). Expressed in all tissues examined and in many cell types, including bone marrow stroma, fibroblast, B-cell precursors, lymphoma and erythroleukemia.2 Publications

Gene expression databases

BgeeiENSMUSG00000030987.
CleanExiMM_STIM1.
ExpressionAtlasiP70302. baseline and differential.
GenevisibleiP70302. MM.

Interactioni

Subunit structurei

Monomer in the presence of Ca2+. It oligomerizes in absence of Ca2+. Forms homooligomers and heterooligomers with STIM2. Interacts (via the transmembrane region and the SOAR/CAD domain) with SPPL3; the interaction promotes the binding of STIM1 to ORAI1. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+. Forms a complex with CRACR2A/EFCAB4B and ORAI1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Interacts with ASPH. Interacts with SLC35G1; intracellular Ca2+-dependent. May interact with ATP1A1, ATP2A2, ATP2B1, ATP2B4, KPNB1 and XPO1; through SLC35G1. Interacts with TMEM203. Interacts with TMEM110/STIMATE, promoting STIM1 conformational switch (By similarity). Interacts with TMEM178A (PubMed:26644563).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8402335,EBI-8402335

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule plus-end binding Source: UniProtKB
  • protease binding Source: MGI

Protein-protein interaction databases

DIPiDIP-48770N.
IntActiP70302. 2 interactors.
MINTiMINT-4135875.
STRINGi10090.ENSMUSP00000033289.

Structurei

3D structure databases

ProteinModelPortaliP70302.
SMRiP70302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 98EF-handAdd BLAST36
Domaini132 – 200SAMPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni334 – 444SOAR/CADBy similarityAdd BLAST111

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili248 – 442By similarityAdd BLAST195

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi642 – 645Microtubule tip localization signal4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi270 – 336Glu-richAdd BLAST67
Compositional biasi601 – 629Pro/Ser-richAdd BLAST29
Compositional biasi672 – 685Lys-richAdd BLAST14

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity
The STIM1 Orai1-activating region/CRAC-activating domain (SOAR/CAD) mediates interaction with ORAI1 to activate the channel.By similarity

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
GeneTreeiENSGT00390000000214.
HOGENOMiHOG000261647.
HOVERGENiHBG054652.
InParanoidiP70302.
KOiK16059.
OrthoDBiEOG091G0CO0.
TreeFamiTF313487.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
InterProiView protein in InterPro
IPR001660. SAM.
IPR013761. SAM/pointed_sf.
IPR032393. SOAR.
IPR030463. STM1.
PANTHERiPTHR15136:SF9. PTHR15136:SF9. 1 hit.
PfamiView protein in Pfam
PF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
SMARTiView protein in SMART
SM00454. SAM. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiView protein in PROSITE
PS50105. SAM_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70302-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVCARLALW LLWGLLLHQG QSLSHSHSEK NTGASSGATS EESTEAEFCR
60 70 80 90 100
IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD
110 120 130 140 150
PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVIQW LITYVELPQY
160 170 180 190 200
EETFRKLQLT GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF
210 220 230 240 250
GPPLLTRHNH LKDFMLVVSI VIGVGGCWFA YIQNRYSKEH MKKMMKDLEG
260 270 280 290 300
LHRAEQSLHD LQERLHKAQE EHRTVEVEKV HLEKKLRDEI NLAKQEAQRL
310 320 330 340 350
KELREGTENE RSRQKYAEEE LEQVREALRK AEKELESHSS WYAPEALQKW
360 370 380 390 400
LQLTHEVEVQ YYNIKKQNAE RQLLVAKEGA EKIKKKRNTL FGTFHVAHSS
410 420 430 440 450
SLDDVDHKIL TAKQALSEVT AALRERLHRW QQIEILCGFQ IVNNPGIHSL
460 470 480 490 500
VAALNIDPSW MGSTRPNPAH FIMTDDVDDM DEEIVSPLSM QSPSLQSSVR
510 520 530 540 550
QRLTEPQLGL GSQRDLTHSD SESSLHMSDR QRVAPKPPQM GRAADEALNA
560 570 580 590 600
MPSNGSHRLI EGVHPGSLVE KLPDSPALAK KTFMALNHGL DKAHSLMELN
610 620 630 640 650
PSVPPGGSPL LDSSHSLSPS SPDPDTPSPV GDNRALQGSR NTRIPHLAGK
660 670 680
KAMAEEDNGS IGEETDSSPG RKKFPLKIFK KPLKK
Length:685
Mass (Da):77,567
Last modified:July 27, 2011 - v2
Checksum:iF4DADF67436790FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331A → S in AAC52715 (PubMed:8707854).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47323 mRNA. Translation: AAC52715.1.
AK041944 mRNA. Translation: BAC31106.1.
CH466531 Genomic DNA. Translation: EDL16597.1.
BC021644 mRNA. Translation: AAH21644.1.
CCDSiCCDS21530.1.
RefSeqiNP_033313.2. NM_009287.4.
UniGeneiMm.645.

Genome annotation databases

EnsembliENSMUST00000033289; ENSMUSP00000033289; ENSMUSG00000030987.
GeneIDi20866.
KEGGimmu:20866.
UCSCiuc009irm.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSTIM1_MOUSE
AccessioniPrimary (citable) accession number: P70302
Secondary accession number(s): Q8K1E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot