P70298 (CUX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein cut-like 2

Alternative name(s):
Homeobox protein Cux-2

Gene names

Name:	Cux2
Synonyms:	Cutl2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a transcription factor involved in neural specification. Binds to DNA in a sequence-specific manner.
Subcellular location	Nucleus.
Tissue specificity	Restricted to neural tissues. Expressed exclusively in the central and peripheral nervous systems.
Sequence similarities	Belongs to the CUT homeobox family. Contains 3 CUT DNA-binding domains. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil Homeobox Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to organic substance Inferred from direct assay PubMed 18794345. Source: MGI negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 15656993. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 15656993PubMed 17412818. Source: MGI
Molecular_function	DNA binding Inferred from direct assay PubMed 15656993. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1426	1426	Homeobox protein cut-like 2		PRO_0000202397
Regions
	DNA binding	482 – 569	88	CUT 1
	DNA binding	828 – 915	88	CUT 2
	DNA binding	983 – 1070	88	CUT 3
	DNA binding	1113 – 1172	60	Homeobox
	Coiled coil	131 – 311	181	Potential
	Coiled coil	587 – 655	69	Potential
Amino acid modifications
	Modified residue	81	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	199	1	S → G in AAC52762. Ref.1
	Sequence conflict	209	1	S → N in AAC52762. Ref.1
	Sequence conflict	324	1	T → M in AAC52762. Ref.1
	Sequence conflict	410	1	T → N in AAC52762. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70298 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 485B87D1C88D2577	FASTA	1,426	154,664
	10 20 30 40 50 60 MVAPVLKSFQ AEVVALSKRS REAEAAFLSV YKQLIEAPDP VPSFEVARTL DDRLQRPSFD 70 80 90 100 110 120 PSGQRLQDVH IAWKRCPEPP SAREQNEGTC PTGHTPANGN HLPGPEDTLV TDTLLQKNEA 130 140 150 160 170 180 ERQKGLQEVH ITLAARLGEA EEKIKVLHSA LKATQTELLE LRRKYDEEAA SKADEVGLIM 190 200 210 220 230 240 TNLEKANQRA EAAQREVESL REQLASVNSS IRLACCSPQG PSGEKVSFAL CSGPRLEAAL 250 260 270 280 290 300 ASKDREILRL LKDAQQLRHS LQELEEVSAN QIADLERQLA AKSEAIEKLQ EKLEAQADYE 310 320 330 340 350 360 EIKTELSILR AMKLASSTCS LPQTLAKPDD PLLVAKDVFF PTQKFLLEKP ALLASPEEDP 370 380 390 400 410 420 SEDDSIKGSL GTEPPYPPQL PPPPGPEDPL SPSPAQPLLG PSLGPDGPRT FSLSPFPSLA 430 440 450 460 470 480 PGERLAGDSL LSKHMMGPAA FKGETGNLLA FPPTFYGGAK PPSAPAASVP CPEPTGAPEA 490 500 510 520 530 540 VDGAGPEEEQ LDTAEIAFQV KEQLLKHNIG QRVFGHYVLG LSQGSVSEIL ARPKPWRKLT 550 560 570 580 590 600 VKGKEPFIKM KQFLSDEQNV LALRTIQVRQ RGSITPRIRT PETGSDDAIK SILEQAKKEI 610 620 630 640 650 660 ESQKGGESKN SPASVSIPNG TASSSTSEDA IKNILEQARR EMQAQQQALL EMESGPRGRS 670 680 690 700 710 720 VPPSPPERPS PATASQNGAL TCVKQEDGGG GSGSSSTVQA PLAVLSPAAF VQRIIRKVKS 730 740 750 760 770 780 EIGDAGYFDH HWASDRGLLS RPYASVSPSL SSSSSYSGQP NGRAWPRGDE ATIAPEDEAA 790 800 810 820 830 840 MGEDEAPRVG ELKAEAGAPE VGGGRLPYYP AYVPRTLKPT VPPLTPEQYE LYMYREVDTL 850 860 870 880 890 900 ELTRQVKEKL AKNGICQRIF GEKVLGLSQG SVSDMLSRPK PWSKLTQKGR EPFIRMQLWL 910 920 930 940 950 960 SDQLGQGQGQ APTQQPSASQ ASPTEPTSSP SPPPSPTEPE KTSQEPLGLS LESSKENQQP 970 980 990 1000 1010 1020 EGRASSSLGG KPFSSSQAAG GIQEMVAMSP ELDTYSITKR VKEVLTDNNL GQRLFGESIL 1030 1040 1050 1060 1070 1080 GLTQGSVSDL LSRPKPWHKL SLKGREPFVR MQLWLSDPHN VEKLRDMKKL EKKAYLKRRY 1090 1100 1110 1120 1130 1140 GLIGTGSDSE SPAAHSECPS PCLQPQELSL MQAKKPRVVL APAEKEALRK AYQLEPYPSQ 1150 1160 1170 1180 1190 1200 QTIELLSFQL NLKTNTVINW FHNYRSRMRR EMLVEGTQDD PDFDPSGGPN VLTPGHTHRE 1210 1220 1230 1240 1250 1260 PTPQSPDSET EDQKPPMKSL ELQEPEGPLQ RAAPDRALVK IKQEEGLEVD GDSQPQDVGD 1270 1280 1290 1300 1310 1320 PDRGQDGPKE EHTHPLGNSD LSELAPGPFL SGTPNPDCPS LHNPQEKGTG EQVHSEPLSF 1330 1340 1350 1360 1370 1380 KSTSESSCCS LEGPPNSPSV ISSPDLTTCV SPAPSSSAPI SPSLPGAPPA KVPSTSPTGD 1390 1400 1410 1420 TAAALHPSTK VNPNLQRRHE KMANLNSIIY RLERAANREE VLEWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure, neural-specific expression, and chromosomal localization of Cux-2, a second murine homeobox gene related to Drosophila cut." Quaggin S.E., Vanden Heuvel G.B., Golden K., Bodmer R., Igarashi P. J. Biol. Chem. 271:22624-22634(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U45665 mRNA. Translation: AAC52762.1. BC065113 mRNA. Translation: AAH65113.1.
IPI	IPI00323964.
PIR	T30817.
RefSeq	NP_031830.2. NM_007804.2.
UniGene	Mm.156172. Mm.332661.
3D structure databases
ProteinModelPortal	P70298.
SMR	P70298. Positions 487-569, 828-912, 977-1165.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70298. 1 interaction.
PTM databases
PhosphoSite	P70298.
Proteomic databases
PRIDE	P70298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000086317; ENSMUSP00000083497; ENSMUSG00000042589. ENSMUST00000111752; ENSMUSP00000107381; ENSMUSG00000042589. ENSMUST00000168288; ENSMUSP00000130302; ENSMUSG00000042589.
GeneID	13048.
KEGG	mmu:13048.
Organism-specific databases
CTD	23316.
MGI	MGI:107321. Cux2.
Phylogenomic databases
eggNOG	NOG325167.
GeneTree	ENSGT00530000063019.
HOGENOM	HOG000143386.
HOVERGEN	HBG051268.
InParanoid	Q6P1E6.
KO	K09313.
OMA	LVFPPAF.
OrthoDB	EOG4K9BBG.
Gene expression databases
ArrayExpress	P70298.
Bgee	P70298.
CleanEx	MM_CUX2.
Genevestigator	P70298.
GermOnline	ENSMUSG00000042589. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 3 hits.
InterPro	IPR003350. Hmoeo_CUT. IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. [Graphical view]
Pfam	PF02376. CUT. 3 hits. PF00046. Homeobox. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 3 hits.
PROSITE	PS51042. CUT. 3 hits. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282958.
SOURCE	Search...

Entry information

Entry name

CUX2_MOUSE

Accession

Primary (citable) accession number: P70298
Secondary accession number(s): Q6P1E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program