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Protein

Signal transducing adapter molecule 1

Gene

Stam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.
R-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 1
Short name:
STAM-1
Gene namesi
Name:Stam
Synonyms:Stam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1329014. Stam.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display disappearance of hippocampal CA3 pyramidal neurons as well as growth retardation in the third week after birth. Adult mice lacking Stam and Stam2 due to inducible gene targeting exhibit significant reduction in T-cell development in the thymus and profound reduction in the peripheral mature T-cells.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 548547Signal transducing adapter molecule 1PRO_0000190146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei156 – 1561PhosphoserineBy similarity
Modified residuei198 – 1981PhosphotyrosineBy similarity
Modified residuei381 – 3811PhosphotyrosineBy similarity
Modified residuei384 – 3841PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70297.
PaxDbiP70297.
PeptideAtlasiP70297.
PRIDEiP70297.

PTM databases

iPTMnetiP70297.
PhosphoSiteiP70297.

Expressioni

Tissue specificityi

Ubiquitously expressed. Enriched expression in synaptic vesicles.1 Publication

Developmental stagei

No change during brain development.

Gene expression databases

BgeeiENSMUSG00000026718.
ExpressionAtlasiP70297. baseline and differential.
GenevisibleiP70297. MM.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS (PubMed:19278655). Probably part of a complex at least composed of HSG, STAM and EPS15. Interacts with STAMBP/AMSH. Interacts with PDGFRB (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi203519. 1 interaction.
IntActiP70297. 1 interaction.
MINTiMINT-96905.
STRINGi10090.ENSMUSP00000100025.

Structurei

3D structure databases

ProteinModelPortaliP70297.
SMRiP70297. Positions 4-143, 211-267, 301-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 143128VHSPROSITE-ProRule annotationAdd
BLAST
Domaini171 – 19020UIMPROSITE-ProRule annotationAdd
BLAST
Domaini210 – 26960SH3PROSITE-ProRule annotationAdd
BLAST
Domaini369 – 38618ITAMAdd
BLAST

Domaini

The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.By similarity

Sequence similaritiesi

Belongs to the STAM family.Curated
Contains 1 ITAM domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
HOVERGENiHBG053175.
InParanoidiP70297.
KOiK04705.
OMAiWANCEER.
OrthoDBiEOG091G0H1Y.
PhylomeDBiP70297.
TreeFamiTF315007.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFATNPFD QDVEKATSEL NTAEDWGLIL DICDKVGQSR TGPKDCLRSI
60 70 80 90 100
MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG
110 120 130 140 150
HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA
160 170 180 190 200
EQAKASPALV AKDPGTVATK KEEEDLAKAI ELSLKEQRQQ SAPVSTLYPS
210 220 230 240 250
TSNLLTNHQH EGRKVRAVYD FEAAEDNELT FKAGEIITVL DDSDPNWWKG
260 270 280 290 300
ETHQGVGLFP SNFVTADLTA EPEMIKTEKK TVQFNDDVQI ETIEPEPEPA
310 320 330 340 350
FIDEDKMDQL LQMLQSTDPS DNQPDLPELL HLEAMCHQMG PLIDEKLEDI
360 370 380 390 400
DRKHSELSEL NVKVMEALSL YTKLMNEDPM YSMYAKLQSQ QYYLQSSAVS
410 420 430 440 450
ASQVYPGPAQ SGTYLVAGSA QMTHLQSYSL PPEQLSSISQ GAVPSSANQA
460 470 480 490 500
LPSQQTQASY PNAMVSSVQG NSYPSQASIY SPPAAAAAAA AAAVVPVPVP
510 520 530 540
ADVTIYQNAG PTMSQVPNYT LTSSTLPQTG GSQQPPQPQQ AYSQKALL
Length:548
Mass (Da):59,771
Last modified:January 23, 2007 - v3
Checksum:i1149D4F9F540C8B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43900 mRNA. Translation: AAC52840.1.
BC044666 mRNA. Translation: AAH44666.1.
BC055326 mRNA. Translation: AAH55326.1.
CCDSiCCDS15698.1.
PIRiJC4917.
RefSeqiNP_035614.1. NM_011484.2.
UniGeneiMm.273174.

Genome annotation databases

EnsembliENSMUST00000102960; ENSMUSP00000100025; ENSMUSG00000026718.
GeneIDi20844.
KEGGimmu:20844.
UCSCiuc008ikh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43900 mRNA. Translation: AAC52840.1.
BC044666 mRNA. Translation: AAH44666.1.
BC055326 mRNA. Translation: AAH55326.1.
CCDSiCCDS15698.1.
PIRiJC4917.
RefSeqiNP_035614.1. NM_011484.2.
UniGeneiMm.273174.

3D structure databases

ProteinModelPortaliP70297.
SMRiP70297. Positions 4-143, 211-267, 301-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203519. 1 interaction.
IntActiP70297. 1 interaction.
MINTiMINT-96905.
STRINGi10090.ENSMUSP00000100025.

PTM databases

iPTMnetiP70297.
PhosphoSiteiP70297.

Proteomic databases

EPDiP70297.
PaxDbiP70297.
PeptideAtlasiP70297.
PRIDEiP70297.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102960; ENSMUSP00000100025; ENSMUSG00000026718.
GeneIDi20844.
KEGGimmu:20844.
UCSCiuc008ikh.1. mouse.

Organism-specific databases

CTDi8027.
MGIiMGI:1329014. Stam.

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
HOVERGENiHBG053175.
InParanoidiP70297.
KOiK04705.
OMAiWANCEER.
OrthoDBiEOG091G0H1Y.
PhylomeDBiP70297.
TreeFamiTF315007.

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.
R-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiStam. mouse.
PROiP70297.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026718.
ExpressionAtlasiP70297. baseline and differential.
GenevisibleiP70297. MM.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTAM1_MOUSE
AccessioniPrimary (citable) accession number: P70297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.