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Protein

Signal transducing adapter molecule 1

Gene

Stam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-182971 EGFR downregulation
R-MMU-5689901 Metalloprotease DUBs
R-MMU-6807004 Negative regulation of MET activity
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 1
Short name:
STAM-1
Gene namesi
Name:Stam
Synonyms:Stam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1329014 Stam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display disappearance of hippocampal CA3 pyramidal neurons as well as growth retardation in the third week after birth. Adult mice lacking Stam and Stam2 due to inducible gene targeting exhibit significant reduction in T-cell development in the thymus and profound reduction in the peripheral mature T-cells.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901461 – 548Signal transducing adapter molecule 1Add BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineBy similarity1
Modified residuei198PhosphotyrosineBy similarity1
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei381PhosphotyrosineBy similarity1
Modified residuei384PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB (By similarity).By similarity
Ubiquitinated by ITCH.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP70297
PaxDbiP70297
PeptideAtlasiP70297
PRIDEiP70297

PTM databases

iPTMnetiP70297
PhosphoSitePlusiP70297

Expressioni

Tissue specificityi

Ubiquitously expressed. Enriched expression in synaptic vesicles.1 Publication

Developmental stagei

No change during brain development.

Gene expression databases

BgeeiENSMUSG00000026718
ExpressionAtlasiP70297 baseline and differential
GenevisibleiP70297 MM

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS (PubMed:19278655). Probably part of a complex at least composed of HSG, STAM and EPS15 (By similarity). Found in a complex with HGS and E3 ligase ITCH and DTX3L (By similarity). Interacts with E3 ligase DTX3L; the interaction brings together STAM and HSG, promotes their recruitment to early endosomes and decreases STAM and HGS ubiquitination by ITCH (By similarity). Interacts with STAMBP/AMSH (By similarity). Interacts with PDGFRB (By similarity). Interacts with LITAF; the interaction is direct (By similarity). Identified in a complex with HGS and LITAF (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203519, 2 interactors
IntActiP70297, 1 interactor
MINTiP70297
STRINGi10090.ENSMUSP00000100025

Structurei

3D structure databases

ProteinModelPortaliP70297
SMRiP70297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 143VHSPROSITE-ProRule annotationAdd BLAST128
Domaini171 – 190UIMPROSITE-ProRule annotationAdd BLAST20
Domaini210 – 269SH3PROSITE-ProRule annotationAdd BLAST60
Domaini369 – 386ITAMAdd BLAST18

Domaini

The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.By similarity

Sequence similaritiesi

Belongs to the STAM family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199 Eukaryota
ENOG410XTSY LUCA
GeneTreeiENSGT00870000136415
HOVERGENiHBG053175
InParanoidiP70297
KOiK04705
OMAiGIMEQAY
OrthoDBiEOG091G0H1Y
PhylomeDBiP70297
TreeFamiTF315007

Family and domain databases

CDDicd11964 SH3_STAM1, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR008942 ENTH_VHS
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035657 STAM1_SH3
IPR003903 UIM_dom
IPR002014 VHS_dom
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PF02809 UIM, 1 hit
PF00790 VHS, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50330 UIM, 1 hit
PS50179 VHS, 1 hit

Sequencei

Sequence statusi: Complete.

P70297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFATNPFD QDVEKATSEL NTAEDWGLIL DICDKVGQSR TGPKDCLRSI
60 70 80 90 100
MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG
110 120 130 140 150
HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA
160 170 180 190 200
EQAKASPALV AKDPGTVATK KEEEDLAKAI ELSLKEQRQQ SAPVSTLYPS
210 220 230 240 250
TSNLLTNHQH EGRKVRAVYD FEAAEDNELT FKAGEIITVL DDSDPNWWKG
260 270 280 290 300
ETHQGVGLFP SNFVTADLTA EPEMIKTEKK TVQFNDDVQI ETIEPEPEPA
310 320 330 340 350
FIDEDKMDQL LQMLQSTDPS DNQPDLPELL HLEAMCHQMG PLIDEKLEDI
360 370 380 390 400
DRKHSELSEL NVKVMEALSL YTKLMNEDPM YSMYAKLQSQ QYYLQSSAVS
410 420 430 440 450
ASQVYPGPAQ SGTYLVAGSA QMTHLQSYSL PPEQLSSISQ GAVPSSANQA
460 470 480 490 500
LPSQQTQASY PNAMVSSVQG NSYPSQASIY SPPAAAAAAA AAAVVPVPVP
510 520 530 540
ADVTIYQNAG PTMSQVPNYT LTSSTLPQTG GSQQPPQPQQ AYSQKALL
Length:548
Mass (Da):59,771
Last modified:January 23, 2007 - v3
Checksum:i1149D4F9F540C8B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43900 mRNA Translation: AAC52840.1
BC044666 mRNA Translation: AAH44666.1
BC055326 mRNA Translation: AAH55326.1
CCDSiCCDS15698.1
PIRiJC4917
RefSeqiNP_035614.1, NM_011484.2
UniGeneiMm.273174

Genome annotation databases

EnsembliENSMUST00000102960; ENSMUSP00000100025; ENSMUSG00000026718
GeneIDi20844
KEGGimmu:20844
UCSCiuc008ikh.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSTAM1_MOUSE
AccessioniPrimary (citable) accession number: P70297
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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