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Protein

Phosphatidylethanolamine-binding protein 1

Gene

Pebp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity).By similarity
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Protein family/group databases

MEROPSiI51.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name:
PEBP-1
Alternative name(s):
HCNPpp
Cleaved into the following chain:
Gene namesi
Name:Pebp1
Synonyms:Pbp, Pebp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1344408. Pebp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 187186Phosphatidylethanolamine-binding protein 1PRO_0000023275Add
BLAST
Peptidei2 – 1211Hippocampal cholinergic neurostimulating peptidePRO_0000023276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine; in peptide hippocampal cholinergic neurostimulatingBy similarity
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei42 – 421PhosphothreonineBy similarity
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei54 – 541PhosphoserineBy similarity
Modified residuei98 – 981PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP70296.
PaxDbiP70296.
PRIDEiP70296.

2D gel databases

REPRODUCTION-2DPAGEP70296.
UCD-2DPAGEP70296.

PTM databases

PhosphoSiteiP70296.

Expressioni

Tissue specificityi

HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.

Gene expression databases

BgeeiP70296.
CleanExiMM_PEBP1.
ExpressionAtlasiP70296. baseline and differential.
GenevisibleiP70296. MM.

Interactioni

Subunit structurei

Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade (By similarity).By similarity

Protein-protein interaction databases

BioGridi204834. 2 interactions.
IntActiP70296. 5 interactions.
MINTiMINT-1869573.
STRINGi10090.ENSMUSP00000048425.

Structurei

3D structure databases

ProteinModelPortaliP70296.
SMRiP70296. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 13442Interaction with RAF1By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG146517.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP70296.
OrthoDBiEOG7P02K3.
PhylomeDBiP70296.
TreeFamiTF315074.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR001858. Phosphotidylethanolamine-bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADISQWAG PLCLQEVDEP PQHALRVDYA GVTVDELGKV LTPTQVMNRP
60 70 80 90 100
SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSDYVGSG PPSGTGLHRY VWLVYEQEQP LSCDEPILSN KSGDNRGKFK
160 170 180
VETFRKKYNL GAPVAGTCYQ AEWDDYVPKL YEQLSGK
Length:187
Mass (Da):20,830
Last modified:January 23, 2007 - v3
Checksum:i4059C7C6E4A8BAA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti116 – 1161G → S in AAB06983 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43206 mRNA. Translation: AAB06983.1.
AF300422 mRNA. Translation: AAG25635.1.
AB046417 mRNA. Translation: BAB03276.1.
AK088212 mRNA. Translation: BAC40214.1.
BC008169 mRNA. Translation: AAH08169.1.
BC083063 mRNA. Translation: AAH83063.1.
CCDSiCCDS39234.1.
PIRiPN0043.
RefSeqiNP_061346.2. NM_018858.2.
UniGeneiMm.195898.
Mm.338476.
Mm.371595.

Genome annotation databases

EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
GeneIDi23980.
KEGGimmu:23980.
UCSCiuc008zfl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43206 mRNA. Translation: AAB06983.1.
AF300422 mRNA. Translation: AAG25635.1.
AB046417 mRNA. Translation: BAB03276.1.
AK088212 mRNA. Translation: BAC40214.1.
BC008169 mRNA. Translation: AAH08169.1.
BC083063 mRNA. Translation: AAH83063.1.
CCDSiCCDS39234.1.
PIRiPN0043.
RefSeqiNP_061346.2. NM_018858.2.
UniGeneiMm.195898.
Mm.338476.
Mm.371595.

3D structure databases

ProteinModelPortaliP70296.
SMRiP70296. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204834. 2 interactions.
IntActiP70296. 5 interactions.
MINTiMINT-1869573.
STRINGi10090.ENSMUSP00000048425.

Protein family/group databases

MEROPSiI51.002.

PTM databases

PhosphoSiteiP70296.

2D gel databases

REPRODUCTION-2DPAGEP70296.
UCD-2DPAGEP70296.

Proteomic databases

MaxQBiP70296.
PaxDbiP70296.
PRIDEiP70296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
GeneIDi23980.
KEGGimmu:23980.
UCSCiuc008zfl.1. mouse.

Organism-specific databases

CTDi5037.
MGIiMGI:1344408. Pebp1.

Phylogenomic databases

eggNOGiNOG146517.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP70296.
OrthoDBiEOG7P02K3.
PhylomeDBiP70296.
TreeFamiTF315074.

Miscellaneous databases

NextBioi303865.
PROiP70296.
SOURCEiSearch...

Gene expression databases

BgeeiP70296.
CleanExiMM_PEBP1.
ExpressionAtlasiP70296. baseline and differential.
GenevisibleiP70296. MM.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR001858. Phosphotidylethanolamine-bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mouse phosphatidylethanolamine binding protein gene."
    Lin B., Frischauf A.-M.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Increased expression of hippocampal cholinergic neurostimulating peptide-related components and their messenger RNAs in the hippocampus of aged senescence-accelerated mice."
    Matsukawa N., Tooyama I., Kimura H., Yamamoto T., Tsugu Y., Oomura Y., Ojika K.
    Neuroscience 88:79-92(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-12.
    Strain: BALB/c.
    Tissue: Brain.
  3. Matsukawa N.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The phosphatidylethanolamine-binding protein is the prototype of a novel family of serine protease inhibitors."
    Hengst U., Albrecht H., Hess D., Monard D.
    J. Biol. Chem. 276:535-540(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-39; 63-76; 94-141 AND 158-180.
    Strain: C57BL/6.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and NMRI.
    Tissue: Mammary gland and Retina.
  7. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 63-76 AND 94-119, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.

Entry informationi

Entry nameiPEBP1_MOUSE
AccessioniPrimary (citable) accession number: P70296
Secondary accession number(s): Q9D8G9, Q9JJ58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.