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P70296

- PEBP1_MOUSE

UniProt

P70296 - PEBP1_MOUSE

Protein

Phosphatidylethanolamine-binding protein 1

Gene

Pebp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation By similarity.By similarity
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. lipid binding Source: UniProtKB-KW
    3. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. aging Source: Ensembl
    2. brain development Source: Ensembl
    3. eating behavior Source: Ensembl
    4. MAPK cascade Source: Ensembl
    5. negative regulation of MAPK cascade Source: Ensembl
    6. negative regulation of protein phosphorylation Source: Ensembl
    7. positive regulation of acetylcholine metabolic process Source: Ensembl
    8. positive regulation of cAMP-mediated signaling Source: Ensembl
    9. positive regulation of mitosis Source: Ensembl
    10. regulation of neurotransmitter levels Source: Ensembl
    11. regulation of the force of heart contraction Source: Ensembl
    12. response to activity Source: Ensembl
    13. response to calcium ion Source: Ensembl
    14. response to cAMP Source: Ensembl
    15. response to corticosterone Source: Ensembl
    16. response to drug Source: Ensembl
    17. response to electrical stimulus Source: Ensembl
    18. response to ethanol Source: Ensembl
    19. response to heat Source: Ensembl
    20. response to oxidative stress Source: Ensembl
    21. response to toxic substance Source: Ensembl
    22. response to wounding Source: Ensembl
    23. sperm capacitation Source: MGI

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Ligandi

    ATP-binding, Lipid-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiI51.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylethanolamine-binding protein 1
    Short name:
    PEBP-1
    Alternative name(s):
    HCNPpp
    Cleaved into the following chain:
    Gene namesi
    Name:Pebp1
    Synonyms:Pbp, Pebp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1344408. Pebp1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical part of cell Source: Ensembl
    2. axon terminus Source: Ensembl
    3. cell surface Source: MGI
    4. extracellular space Source: Ensembl
    5. Golgi apparatus Source: Ensembl
    6. mitochondrial outer membrane Source: Ensembl
    7. neuronal cell body Source: Ensembl
    8. rough endoplasmic reticulum Source: Ensembl
    9. synaptic vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 187186Phosphatidylethanolamine-binding protein 1PRO_0000023275Add
    BLAST
    Peptidei2 – 1211Hippocampal cholinergic neurostimulating peptidePRO_0000023276Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine; in peptide hippocampal cholinergic neurostimulatingBy similarity
    Modified residuei42 – 421PhosphothreonineBy similarity
    Modified residuei52 – 521PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiP70296.
    PaxDbiP70296.
    PRIDEiP70296.

    2D gel databases

    REPRODUCTION-2DPAGEP70296.
    UCD-2DPAGEP70296.

    PTM databases

    PhosphoSiteiP70296.

    Expressioni

    Tissue specificityi

    HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.

    Gene expression databases

    ArrayExpressiP70296.
    BgeeiP70296.
    CleanExiMM_PEBP1.
    GenevestigatoriP70296.

    Interactioni

    Subunit structurei

    Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204834. 1 interaction.
    IntActiP70296. 5 interactions.
    MINTiMINT-1869573.
    STRINGi10090.ENSMUSP00000107604.

    Structurei

    3D structure databases

    ProteinModelPortaliP70296.
    SMRiP70296. Positions 1-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 13442Interaction with RAF1By similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG146517.
    HOGENOMiHOG000237655.
    HOVERGENiHBG008165.
    OrthoDBiEOG7P02K3.
    PhylomeDBiP70296.
    TreeFamiTF315074.

    Family and domain databases

    Gene3Di3.90.280.10. 1 hit.
    InterProiIPR001858. Phosphotidylethanolamine-bd_CS.
    IPR008914. PtdEtn-bd_prot_PEBP.
    [Graphical view]
    PfamiPF01161. PBP. 1 hit.
    [Graphical view]
    SUPFAMiSSF49777. SSF49777. 1 hit.
    PROSITEiPS01220. PBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAADISQWAG PLCLQEVDEP PQHALRVDYA GVTVDELGKV LTPTQVMNRP    50
    SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG 100
    TVLSDYVGSG PPSGTGLHRY VWLVYEQEQP LSCDEPILSN KSGDNRGKFK 150
    VETFRKKYNL GAPVAGTCYQ AEWDDYVPKL YEQLSGK 187
    Length:187
    Mass (Da):20,830
    Last modified:January 23, 2007 - v3
    Checksum:i4059C7C6E4A8BAA0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti116 – 1161G → S in AAB06983. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43206 mRNA. Translation: AAB06983.1.
    AF300422 mRNA. Translation: AAG25635.1.
    AB046417 mRNA. Translation: BAB03276.1.
    AK088212 mRNA. Translation: BAC40214.1.
    BC008169 mRNA. Translation: AAH08169.1.
    BC083063 mRNA. Translation: AAH83063.1.
    CCDSiCCDS39234.1.
    PIRiPN0043.
    RefSeqiNP_061346.2. NM_018858.2.
    UniGeneiMm.195898.
    Mm.338476.
    Mm.371595.

    Genome annotation databases

    EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
    GeneIDi23980.
    KEGGimmu:23980.
    UCSCiuc008zfl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43206 mRNA. Translation: AAB06983.1 .
    AF300422 mRNA. Translation: AAG25635.1 .
    AB046417 mRNA. Translation: BAB03276.1 .
    AK088212 mRNA. Translation: BAC40214.1 .
    BC008169 mRNA. Translation: AAH08169.1 .
    BC083063 mRNA. Translation: AAH83063.1 .
    CCDSi CCDS39234.1.
    PIRi PN0043.
    RefSeqi NP_061346.2. NM_018858.2.
    UniGenei Mm.195898.
    Mm.338476.
    Mm.371595.

    3D structure databases

    ProteinModelPortali P70296.
    SMRi P70296. Positions 1-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204834. 1 interaction.
    IntActi P70296. 5 interactions.
    MINTi MINT-1869573.
    STRINGi 10090.ENSMUSP00000107604.

    Protein family/group databases

    MEROPSi I51.002.

    PTM databases

    PhosphoSitei P70296.

    2D gel databases

    REPRODUCTION-2DPAGE P70296.
    UCD-2DPAGE P70296.

    Proteomic databases

    MaxQBi P70296.
    PaxDbi P70296.
    PRIDEi P70296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036951 ; ENSMUSP00000048425 ; ENSMUSG00000032959 .
    GeneIDi 23980.
    KEGGi mmu:23980.
    UCSCi uc008zfl.1. mouse.

    Organism-specific databases

    CTDi 5037.
    MGIi MGI:1344408. Pebp1.

    Phylogenomic databases

    eggNOGi NOG146517.
    HOGENOMi HOG000237655.
    HOVERGENi HBG008165.
    OrthoDBi EOG7P02K3.
    PhylomeDBi P70296.
    TreeFami TF315074.

    Miscellaneous databases

    NextBioi 303865.
    PROi P70296.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70296.
    Bgeei P70296.
    CleanExi MM_PEBP1.
    Genevestigatori P70296.

    Family and domain databases

    Gene3Di 3.90.280.10. 1 hit.
    InterProi IPR001858. Phosphotidylethanolamine-bd_CS.
    IPR008914. PtdEtn-bd_prot_PEBP.
    [Graphical view ]
    Pfami PF01161. PBP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49777. SSF49777. 1 hit.
    PROSITEi PS01220. PBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of mouse phosphatidylethanolamine binding protein gene."
      Lin B., Frischauf A.-M.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Increased expression of hippocampal cholinergic neurostimulating peptide-related components and their messenger RNAs in the hippocampus of aged senescence-accelerated mice."
      Matsukawa N., Tooyama I., Kimura H., Yamamoto T., Tsugu Y., Oomura Y., Ojika K.
      Neuroscience 88:79-92(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-12.
      Strain: BALB/c.
      Tissue: Brain.
    3. Matsukawa N.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The phosphatidylethanolamine-binding protein is the prototype of a novel family of serine protease inhibitors."
      Hengst U., Albrecht H., Hess D., Monard D.
      J. Biol. Chem. 276:535-540(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-39; 63-76; 94-141 AND 158-180.
      Strain: C57BL/6.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Thymus.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and NMRI.
      Tissue: Mammary gland and Retina.
    7. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 63-76 AND 94-119, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiPEBP1_MOUSE
    AccessioniPrimary (citable) accession number: P70296
    Secondary accession number(s): Q9D8G9, Q9JJ58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3