P70296 (PEBP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name=PEBP-1

Alternative name(s):
HCNPpp

Cleaved into the following chain:

1. Hippocampal cholinergic neurostimulating peptide
   Short name=HCNP

Gene names

Name:	Pebp1
Synonyms:	Pbp, Pebp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation By similarity. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor By similarity.
Subunit structure	Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341' By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.
Sequence similarities	Belongs to the phosphatidylethanolamine-binding protein family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Lipid-binding Nucleotide-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Acetylation Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: Compara aging Inferred from electronic annotation. Source: Compara brain development Inferred from electronic annotation. Source: Compara eating behavior Inferred from electronic annotation. Source: Compara negative regulation of MAPK cascade Inferred from electronic annotation. Source: Compara negative regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of acetylcholine metabolic process Inferred from electronic annotation. Source: Compara positive regulation of cAMP-mediated signaling Inferred from electronic annotation. Source: Compara positive regulation of mitosis Inferred from electronic annotation. Source: Compara regulation of neurotransmitter levels Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction Inferred from electronic annotation. Source: Compara response to activity Inferred from electronic annotation. Source: Compara response to cAMP Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to corticosterone stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to electrical stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to heat Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from electronic annotation. Source: Compara response to toxic substance Inferred from electronic annotation. Source: Compara response to wounding Inferred from electronic annotation. Source: Compara sperm capacitation Inferred from direct assay PubMed 16183867. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara apical part of cell Inferred from electronic annotation. Source: Compara axon terminus Inferred from electronic annotation. Source: Compara cell surface Inferred from direct assay PubMed 16183867. Source: MGI extracellular space Inferred from electronic annotation. Source: Compara mitochondrial outer membrane Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara rough endoplasmic reticulum Inferred from electronic annotation. Source: Compara synaptic vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lipid binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 187	186	Phosphatidylethanolamine-binding protein 1		PRO_0000023275
	Peptide	2 – 12	11	Hippocampal cholinergic neurostimulating peptide Ref.2		PRO_0000023276
Regions
	Region	93 – 134	42	Interaction with RAF1 By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; in peptide hippocampal cholinergic neurostimulating By similarity
	Modified residue	42	1	Phosphothreonine By similarity
	Modified residue	51	1	Phosphoserine Ref.8
	Modified residue	52	1	Phosphoserine Ref.8
	Modified residue	54	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	116	1	G → S in AAB06983. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70296 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4059C7C6E4A8BAA0	FASTA	187	20,830
	10 20 30 40 50 60 MAADISQWAG PLCLQEVDEP PQHALRVDYA GVTVDELGKV LTPTQVMNRP SSISWDGLDP 70 80 90 100 110 120 GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG TVLSDYVGSG PPSGTGLHRY 130 140 150 160 170 180 VWLVYEQEQP LSCDEPILSN KSGDNRGKFK VETFRKKYNL GAPVAGTCYQ AEWDDYVPKL YEQLSGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of mouse phosphatidylethanolamine binding protein gene." Lin B., Frischauf A.-M. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Increased expression of hippocampal cholinergic neurostimulating peptide-related components and their messenger RNAs in the hippocampus of aged senescence-accelerated mice." Matsukawa N., Tooyama I., Kimura H., Yamamoto T., Tsugu Y., Oomura Y., Ojika K. Neuroscience 88:79-92(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-12. Strain: BALB/c. Tissue: Brain.
[3]	Matsukawa N. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The phosphatidylethanolamine-binding protein is the prototype of a novel family of serine protease inhibitors." Hengst U., Albrecht H., Hess D., Monard D. J. Biol. Chem. 276:535-540(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-39; 63-76; 94-141 AND 158-180. Strain: C57BL/6.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Thymus.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and NMRI. Tissue: Mammary gland and Retina.
[7]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 63-76 AND 94-119, MASS SPECTROMETRY. Tissue: Hippocampus.
[8]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51; SER-52 AND SER-54, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43206 mRNA. Translation: AAB06983.1. AF300422 mRNA. Translation: AAG25635.1. AB046417 mRNA. Translation: BAB03276.1. AK088212 mRNA. Translation: BAC40214.1. BC008169 mRNA. Translation: AAH08169.1. BC083063 mRNA. Translation: AAH83063.1.
IPI	IPI00137730.
PIR	PN0043.
RefSeq	NP_061346.2. NM_018858.2.
UniGene	Mm.195898. Mm.338476. Mm.371595.
3D structure databases
ProteinModelPortal	P70296.
SMR	P70296. Positions 1-187.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70296. 2 interactions.
MINT	MINT-1869573.
STRING	10090.ENSMUSP00000107604.
Protein family/group databases
MEROPS	I51.002.
PTM databases
PhosphoSite	P70296.
2D gel databases
REPRODUCTION-2DPAGE	P70296.
UCD-2DPAGE	P70296.
Proteomic databases
PaxDb	P70296.
PRIDE	P70296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
GeneID	23980.
KEGG	mmu:23980.
UCSC	uc008zfl.1. mouse.
Organism-specific databases
CTD	5037.
MGI	MGI:1344408. Pebp1.
Phylogenomic databases
eggNOG	NOG146517.
HOGENOM	HOG000237655.
HOVERGEN	HBG008165.
OrthoDB	EOG4FFD2T.
Gene expression databases
ArrayExpress	P70296.
Bgee	P70296.
CleanEx	MM_PEBP1.
Genevestigator	P70296.
GermOnline	ENSMUSG00000032959. Mus musculus.
Family and domain databases
Gene3D	3.90.280.10. 1 hit.
InterPro	IPR001858. Phosphotidylethanolamine-bd_CS. IPR008914. PtdEtn-bd_prot_PEBP. [Graphical view]
Pfam	PF01161. PBP. 1 hit. [Graphical view]
SUPFAM	SSF49777. PEBP. 1 hit.
PROSITE	PS01220. PBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	303865.
SOURCE	Search...

Entry information

Entry name

PEBP1_MOUSE

Accession

Primary (citable) accession number: P70296
Secondary accession number(s): Q9D8G9, Q9JJ58

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program