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Protein

Phosphatidylethanolamine-binding protein 1

Gene

Pebp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity).By similarity
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
LigandATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway

Protein family/group databases

MEROPSiI51.002

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name:
PEBP-1
Alternative name(s):
HCNPpp
Cleaved into the following chain:
Gene namesi
Name:Pebp1
Synonyms:Pbp, Pebp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1344408 Pebp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000232752 – 187Phosphatidylethanolamine-binding protein 1Add BLAST186
PeptideiPRO_00000232762 – 12Hippocampal cholinergic neurostimulating peptideAdd BLAST11

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; in peptide hippocampal cholinergic neurostimulatingBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei42PhosphothreonineBy similarity1
Modified residuei51PhosphoserineCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei98PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP70296
PaxDbiP70296
PeptideAtlasiP70296
PRIDEiP70296
TopDownProteomicsiP70296

2D gel databases

REPRODUCTION-2DPAGEiP70296
UCD-2DPAGEiP70296

PTM databases

iPTMnetiP70296
PhosphoSitePlusiP70296
SwissPalmiP70296

Expressioni

Tissue specificityi

HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.

Gene expression databases

BgeeiENSMUSG00000032959
CleanExiMM_PEBP1
ExpressionAtlasiP70296 baseline and differential
GenevisibleiP70296 MM

Interactioni

Subunit structurei

Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204834, 6 interactors
IntActiP70296, 6 interactors
MINTiP70296
STRINGi10090.ENSMUSP00000048425

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 7Combined sources3
Helixi14 – 16Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi26 – 29Combined sources4
Beta strandi32 – 34Combined sources3
Helixi44 – 46Combined sources3
Beta strandi51 – 54Combined sources4
Beta strandi62 – 74Combined sources13
Beta strandi76 – 78Combined sources3
Beta strandi84 – 93Combined sources10
Helixi97 – 99Combined sources3
Beta strandi100 – 104Combined sources5
Beta strandi118 – 126Combined sources9
Helixi151 – 157Combined sources7
Beta strandi164 – 171Combined sources8
Helixi177 – 184Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6ENSX-ray1.30A2-187[»]
ProteinModelPortaliP70296
SMRiP70296
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 134Interaction with RAF1By similarityAdd BLAST42

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3346 Eukaryota
COG1881 LUCA
GeneTreeiENSGT00900000141013
HOGENOMiHOG000237655
HOVERGENiHBG008165
InParanoidiP70296
PhylomeDBiP70296
TreeFamiTF315074

Family and domain databases

CDDicd00866 PEBP_euk, 1 hit
Gene3Di3.90.280.10, 1 hit
InterProiView protein in InterPro
IPR008914 PEBP
IPR036610 PEBP-like_sf
IPR035810 PEBP_euk
IPR001858 Phosphotidylethanolamine-bd_CS
PANTHERiPTHR11362 PTHR11362, 1 hit
PfamiView protein in Pfam
PF01161 PBP, 1 hit
SUPFAMiSSF49777 SSF49777, 1 hit
PROSITEiView protein in PROSITE
PS01220 PBP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADISQWAG PLCLQEVDEP PQHALRVDYA GVTVDELGKV LTPTQVMNRP
60 70 80 90 100
SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSDYVGSG PPSGTGLHRY VWLVYEQEQP LSCDEPILSN KSGDNRGKFK
160 170 180
VETFRKKYNL GAPVAGTCYQ AEWDDYVPKL YEQLSGK
Length:187
Mass (Da):20,830
Last modified:January 23, 2007 - v3
Checksum:i4059C7C6E4A8BAA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116G → S in AAB06983 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43206 mRNA Translation: AAB06983.1
AF300422 mRNA Translation: AAG25635.1
AB046417 mRNA Translation: BAB03276.1
AK088212 mRNA Translation: BAC40214.1
BC008169 mRNA Translation: AAH08169.1
BC083063 mRNA Translation: AAH83063.1
CCDSiCCDS39234.1
PIRiPN0043
RefSeqiNP_061346.2, NM_018858.2
UniGeneiMm.195898
Mm.338476
Mm.371595

Genome annotation databases

EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959
GeneIDi23980
KEGGimmu:23980
UCSCiuc008zfl.1 mouse

Entry informationi

Entry nameiPEBP1_MOUSE
AccessioniPrimary (citable) accession number: P70296
Secondary accession number(s): Q9D8G9, Q9JJ58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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