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Protein

Phosphatidylethanolamine-binding protein 1

Gene

Pebp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity).By similarity
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5675221. Negative regulation of MAPK pathway.

Protein family/group databases

MEROPSiI51.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name:
PEBP-1
Alternative name(s):
HCNPpp
Cleaved into the following chain:
Gene namesi
Name:Pebp1
Synonyms:Pbp, Pebp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1344408. Pebp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000232752 – 187Phosphatidylethanolamine-binding protein 1Add BLAST186
PeptideiPRO_00000232762 – 12Hippocampal cholinergic neurostimulating peptideAdd BLAST11

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; in peptide hippocampal cholinergic neurostimulatingBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei42PhosphothreonineBy similarity1
Modified residuei51PhosphoserineCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei98PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP70296.
PaxDbiP70296.
PeptideAtlasiP70296.
PRIDEiP70296.
TopDownProteomicsiP70296.

2D gel databases

REPRODUCTION-2DPAGEP70296.
UCD-2DPAGEP70296.

PTM databases

iPTMnetiP70296.
PhosphoSitePlusiP70296.
SwissPalmiP70296.

Expressioni

Tissue specificityi

HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.

Gene expression databases

BgeeiENSMUSG00000032959.
CleanExiMM_PEBP1.
ExpressionAtlasiP70296. baseline and differential.
GenevisibleiP70296. MM.

Interactioni

Subunit structurei

Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204834. 2 interactors.
IntActiP70296. 6 interactors.
MINTiMINT-1869573.
STRINGi10090.ENSMUSP00000048425.

Structurei

3D structure databases

ProteinModelPortaliP70296.
SMRiP70296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 134Interaction with RAF1By similarityAdd BLAST42

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3346. Eukaryota.
COG1881. LUCA.
GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP70296.
PhylomeDBiP70296.
TreeFamiTF315074.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADISQWAG PLCLQEVDEP PQHALRVDYA GVTVDELGKV LTPTQVMNRP
60 70 80 90 100
SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSDYVGSG PPSGTGLHRY VWLVYEQEQP LSCDEPILSN KSGDNRGKFK
160 170 180
VETFRKKYNL GAPVAGTCYQ AEWDDYVPKL YEQLSGK
Length:187
Mass (Da):20,830
Last modified:January 23, 2007 - v3
Checksum:i4059C7C6E4A8BAA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116G → S in AAB06983 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43206 mRNA. Translation: AAB06983.1.
AF300422 mRNA. Translation: AAG25635.1.
AB046417 mRNA. Translation: BAB03276.1.
AK088212 mRNA. Translation: BAC40214.1.
BC008169 mRNA. Translation: AAH08169.1.
BC083063 mRNA. Translation: AAH83063.1.
CCDSiCCDS39234.1.
PIRiPN0043.
RefSeqiNP_061346.2. NM_018858.2.
UniGeneiMm.195898.
Mm.338476.
Mm.371595.

Genome annotation databases

EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
GeneIDi23980.
KEGGimmu:23980.
UCSCiuc008zfl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43206 mRNA. Translation: AAB06983.1.
AF300422 mRNA. Translation: AAG25635.1.
AB046417 mRNA. Translation: BAB03276.1.
AK088212 mRNA. Translation: BAC40214.1.
BC008169 mRNA. Translation: AAH08169.1.
BC083063 mRNA. Translation: AAH83063.1.
CCDSiCCDS39234.1.
PIRiPN0043.
RefSeqiNP_061346.2. NM_018858.2.
UniGeneiMm.195898.
Mm.338476.
Mm.371595.

3D structure databases

ProteinModelPortaliP70296.
SMRiP70296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204834. 2 interactors.
IntActiP70296. 6 interactors.
MINTiMINT-1869573.
STRINGi10090.ENSMUSP00000048425.

Protein family/group databases

MEROPSiI51.002.

PTM databases

iPTMnetiP70296.
PhosphoSitePlusiP70296.
SwissPalmiP70296.

2D gel databases

REPRODUCTION-2DPAGEP70296.
UCD-2DPAGEP70296.

Proteomic databases

EPDiP70296.
PaxDbiP70296.
PeptideAtlasiP70296.
PRIDEiP70296.
TopDownProteomicsiP70296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036951; ENSMUSP00000048425; ENSMUSG00000032959.
GeneIDi23980.
KEGGimmu:23980.
UCSCiuc008zfl.1. mouse.

Organism-specific databases

CTDi5037.
MGIiMGI:1344408. Pebp1.

Phylogenomic databases

eggNOGiKOG3346. Eukaryota.
COG1881. LUCA.
GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP70296.
PhylomeDBiP70296.
TreeFamiTF315074.

Enzyme and pathway databases

ReactomeiR-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

PROiP70296.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032959.
CleanExiMM_PEBP1.
ExpressionAtlasiP70296. baseline and differential.
GenevisibleiP70296. MM.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEBP1_MOUSE
AccessioniPrimary (citable) accession number: P70296
Secondary accession number(s): Q9D8G9, Q9JJ58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.