Reviewed, UniProtKB/Swiss-Prot P70295 (AUP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ancient ubiquitous protein 1

Gene names

Name:

Aup1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome By similarity.
Subunit structure	Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cytoplasmic side By similarity.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the AUP1 family. Contains 1 CUE domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Ancient ubiquitous protein 1		PRO_0000020766
Regions
	Topological domain	1 – 20	20	Lumenal Potential
	Transmembrane	21 – 41	21	Signal-anchor for type III membrane protein Potential
	Topological domain	42 – 410	369	Cytoplasmic Potential
	Domain	296 – 338	43	CUE
Amino acid modifications
	Modified residue	292	1	Phosphoserine By similarity
	Modified residue	356	1	Phosphoserine By similarity
	Modified residue	363	1	Phosphoserine By similarity
	Modified residue	367	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70295-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E7D070CEB296BD5B	FASTA	410	46,121
	10 20 30 40 50 60 MEPPPAPGPE RLFDSHRLPS DGFLLLALLL YAPVGLCLLV LRLFLGLHVF LVSCALPDSV 70 80 90 100 110 120 LRRFVVRTMC AVLGLVARQE DSGLRDHRVR VLISNHVTPF DHNIVNLLTT CSTPLLNSPP 130 140 150 160 170 180 SFVCWSRGFM EMDRRVELVE SLKKFCASTR LPPTPLLLFP EEEATNGREG LLRFSSWPFS 190 200 210 220 230 240 IQDVVQPLTL QVQRPLVSVT VSDASWVSEL LWSLFVPFTV YQVRWLHPIR RQLGEESEEF 250 260 270 280 290 300 ALRVQQLVAK ELGQIGTRLT PADKAEHMKR QRHPRLRPQS VQSSFPSPPS PSSDVQLTTL 310 320 330 340 350 360 AHRVKEVLPH VPLNVIQRDL ARTGCVDLTI TNLLEGAVAF MPEDVTEGSQ SPPAPSAPKF 370 380 390 400 410 PSSGLATPQP TALTFAKSSW ARQESLQERK QALYEYARRR FRERQAQEAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13." Jang W., Weber J.S., Bashir R., Bushby K., Meisler M.H. Genomics 36:366-368(1996) [PubMed: 8812468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41736 mRNA. Translation: AAC52839.1. BC016485 mRNA. Translation: AAH16485.1.
IPI	IPI00137725.
UniGene	Mm.386752
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P70295.
PTM databases
PhosphoSite	P70295.
Proteomic databases
PRIDE	P70295.
Genome annotation databases
Ensembl	ENSMUST00000092618; ENSMUSP00000090281; ENSMUSG00000068328; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:107789. Aup1.
Phylogenomic databases
HOVERGEN	P70295.
Gene expression databases
ArrayExpress	P70295.
Bgee	P70295.
CleanEx	MM_AUP1.
Genevestigator	P70295.
GermOnline	ENSMUSG00000068328. Mus musculus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR003892. CUE. [Graphical view]
Pfam	PF02845. CUE. 1 hit. [Graphical view]
SMART	SM00546. CUE. 1 hit. SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS51140. CUE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AUP1_MOUSE

Accession

Primary (citable) accession number: P70295

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)