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P70284 (TGIF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein TGIF1
Alternative name(s):
5'-TG-3'-interacting factor 1
Gene names
Name:Tgif1
Synonyms:Tgif
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities By similarity.

Subunit structure

Interacts with CTBP, SMAD2, SMAD3 and HDAC1 By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the TALE/TGIF homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdetermination of left/right symmetry

Inferred from mutant phenotype PubMed 16705179. Source: MGI

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 17082251. Source: MGI

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 17082251. Source: MGI

negative regulation of retinoic acid receptor signaling pathway

Inferred from mutant phenotype PubMed 16428452. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16428452. Source: MGI

neural tube closure

Inferred from mutant phenotype PubMed 17082251. Source: MGI

nodal signaling pathway

Inferred from genetic interaction PubMed 20040491. Source: MGI

positive regulation of fibroblast proliferation

Inferred from mutant phenotype PubMed 16705179. Source: MGI

positive regulation of neuron differentiation

Inferred from direct assay PubMed 18973577. Source: MGI

regulation of gastrulation

Inferred from genetic interaction PubMed 20040491. Source: MGI

retina development in camera-type eye

Inferred from direct assay PubMed 18973577. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 21503901. Source: MGI

   Molecular_functionchromatin binding

Inferred from direct assay PubMed 16428452. Source: MGI

protein binding

Inferred from physical interaction PubMed 16428452. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70284-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70284-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Homeobox protein TGIF1
PRO_0000049320

Regions

DNA binding35 – 9763Homeobox; TALE-type
Motif24 – 285CTBP-binding motif
Compositional bias36 – 394Poly-Arg

Natural variations

Alternative sequence1 – 2020Missing in isoform 2.
VSP_002297

Experimental info

Sequence conflict351K → M in CAA61896. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 17, 2003. Version 2.
Checksum: 625528713B4C0C42

FASTA27229,573
        10         20         30         40         50         60 
MKSKKGLVAA SGSDSEDEDS MDSPLDLSSS AASGKRRRRG NLPKESVQIL RDWLYEHRYN 

        70         80         90        100        110        120 
AYPSEQEKAL LSQQTHLSTL QVCNWFINAR RRLLPDMLRK DGKDPNQFTI SRRGAKISEA 

       130        140        150        160        170        180 
SSIEAAMGIK NFMPTLEESP FHSCVVGPNP TLGRPVSPKP PSPGSILARP SVICHTTVTA 

       190        200        210        220        230        240 
LKDGPFSLCQ PIGVGQSTDV PQIAPSNFTD TSLVYPEDTC KSGPSPNPQS GLFNTPPPTP 

       250        260        270 
PDLNQDFSGF QLLVDVALKR AAEMELQAKL TA 

« Hide

Isoform 2 [UniParc].

Checksum: 5595D6086BABF19A
Show »

FASTA25227,550

References

« Hide 'large scale' references
[1]"Expression of a novel murine homeobox gene in the developing cerebellar external granular layer during its proliferation."
Bertolino E., Wildt S., Richards G., Clerc R.G.
Dev. Dyn. 205:410-420(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Mammary fibroblast and Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89749 mRNA. Translation: CAA61896.1.
BC005724 mRNA. Translation: AAH05724.1.
BC012700 mRNA. Translation: AAH12700.1.
CCDSCCDS37683.1. [P70284-1]
CCDS50174.1. [P70284-2]
RefSeqNP_001157546.1. NM_001164074.1. [P70284-2]
NP_001157548.1. NM_001164076.1. [P70284-2]
NP_001157549.1. NM_001164077.1. [P70284-2]
NP_033398.2. NM_009372.3. [P70284-1]
XP_006524095.1. XM_006524032.1. [P70284-2]
UniGeneMm.101034.

3D structure databases

ProteinModelPortalP70284.
SMRP70284. Positions 42-119.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204166. 5 interactions.

PTM databases

PhosphoSiteP70284.

Proteomic databases

PRIDEP70284.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000059775; ENSMUSP00000060512; ENSMUSG00000047407. [P70284-1]
ENSMUST00000118283; ENSMUSP00000113192; ENSMUSG00000047407. [P70284-2]
ENSMUST00000172229; ENSMUSP00000127139; ENSMUSG00000047407. [P70284-2]
GeneID21815.
KEGGmmu:21815.
UCSCuc008dlp.2. mouse. [P70284-1]

Organism-specific databases

CTD7050.
MGIMGI:1194497. Tgif1.

Phylogenomic databases

eggNOGNOG308174.
GeneTreeENSGT00680000099964.
HOGENOMHOG000232039.
HOVERGENHBG001143.
InParanoidP70284.
OrthoDBEOG7H7937.
PhylomeDBP70284.

Gene expression databases

ArrayExpressP70284.
BgeeP70284.
CleanExMM_TGIF1.
GenevestigatorP70284.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTGIF1. mouse.
NextBio301216.
PROP70284.
SOURCESearch...

Entry information

Entry nameTGIF1_MOUSE
AccessionPrimary (citable) accession number: P70284
Secondary accession number(s): Q921F9, Q99JS3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 17, 2003
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot