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P70281 (SYCP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptonemal complex protein 3

Short name=SCP-3
Gene names
Name:Sycp3
Synonyms:Scp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for axial elements (AE) in meiotic cells and for assembly of the synaptonemal complex. May be important for testis development and male fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2. Ref.4 Ref.5

Subunit structure

Interacts with SYCP2 By similarity.

Subcellular location

Nucleus By similarity. Chromosome. Note: In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed By similarity. Ref.5

Post-translational modification

Phosphorylated. Ref.5

Disruption phenotype

Homozygous males with null mutations were sterile due to massive apoptotic cell death during meiotic prophase. Failed to form axial/lateral elements and SCs, and the chromosomes in the mutant spermatocytes did not synapse. In contrast, females were fertile and generated healthy offspring. However, they exhibited a sharp reduction in litter size that increased with advancing maternal age. Ref.4

Sequence similarities

Belongs to the XLR/SYCP3 family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Meiosis
Photosynthesis
   Cellular componentChromosome
Nucleus
   DomainCoiled coil
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchiasma assembly

Inferred from direct assay PubMed 12004129. Source: UniProtKB

female meiosis chromosome segregation

Inferred from direct assay PubMed 12004129. Source: UniProtKB

female meiosis chromosome separation

Non-traceable author statement PubMed 15305061. Source: UniProtKB

female meiosis sister chromatid cohesion

Inferred from direct assay PubMed 15870106. Source: UniProtKB

lateral element assembly

Inferred from genetic interaction PubMed 22164254. Source: MGI

meiotic DNA repair synthesis

Inferred from genetic interaction PubMed 22164254. Source: MGI

meiotic nuclear division

Inferred from direct assay PubMed 12091911. Source: MGI

mitosis

Inferred from direct assay PubMed 10809667. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype Ref.4. Source: UniProtKB

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

sperm chromatin condensation

Inferred from genetic interaction PubMed 22164254. Source: MGI

spermatogenesis

Inferred from mutant phenotype Ref.4. Source: UniProtKB

synapsis

Inferred from mutant phenotype PubMed 15870106. Source: MGI

synaptonemal complex assembly

Inferred from direct assay Ref.4. Source: UniProtKB

   Cellular_componentchromosome

Inferred from direct assay PubMed 10794082. Source: MGI

condensed chromosome

Inferred from direct assay PubMed 10809667PubMed 12091911PubMed 12591952PubMed 17784788PubMed 19625504. Source: MGI

condensed nuclear chromosome

Inferred from direct assay PubMed 18316482PubMed 21621532. Source: MGI

lateral element

Inferred from direct assay Ref.4. Source: UniProtKB

male germ cell nucleus

Inferred from direct assay PubMed 17696610. Source: MGI

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 21621532. Source: MGI

synaptonemal complex

Inferred from direct assay PubMed 12815066PubMed 14736746PubMed 15657431PubMed 16428451PubMed 16968740PubMed 17141210PubMed 17696610PubMed 17912366PubMed 18070917PubMed 20551173. Source: MGI

transverse filament

Inferred from direct assay PubMed 15870106. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein heterodimerization activity

Inferred from physical interaction PubMed 16717126. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Synaptonemal complex protein 3
PRO_0000223044

Regions

Coiled coil115 – 252138 Potential
Motif106 – 1094Nuclear localization signal Potential
Compositional bias123 – 250128Gln-rich

Experimental info

Sequence conflict1911F → K Ref.3
Sequence conflict1931M → I Ref.3

Sequences

Sequence LengthMass (Da)Tools
P70281 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E0121D58A8DCD2EA

FASTA25429,347
        10         20         30         40         50         60 
MLRGCGDSDS SPEPLSKHLK MVPGGRKHSG KSGKPPLVDQ PKKAFDFEKD DKDLSGSEED 

        70         80         90        100        110        120 
VADEKAPVID KHGKKRSAGI IEDVGGEVQN MLEKFGADIN KALLAKRKRI EMYTKASFKA 

       130        140        150        160        170        180 
SNQKIEQIWK TQQEEIQKLN NEYSQQFMNV LQQWELDIQK FEEQGEKLSN LFRQQQKIFQ 

       190        200        210        220        230        240 
QSRIVQSQRM FAMKQIHEQF IKSLEDVEKN NDNLFTGTQS ELKKEMAMLQ KKVMMETQQQ 

       250 
EMANVRKSLQ SMLF 

« Hide

References

« Hide 'large scale' references
[1]"The mouse synaptosomal complex protein gene Sycp3 maps to band C of chromosome 10."
Klink A., Lee M., Cooke H.J.
Mamm. Genome 8:376-377(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Liver and Testis.
[2]"The genomic structure of SYCP3, a meiosis-specific gene encoding a protein of the chromosome core."
Botelho R.J., DiNicolo L., Tsao N., Karaiskakis A., Tarsounas M., Moens P.B., Pearlman R.E.
Biochim. Biophys. Acta 1518:294-299(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryonic stem cell.
[4]"The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility."
Yuan L., Liu J.G., Zhao J., Brundell E., Daneholt B., Hoog C.
Mol. Cell 5:73-83(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis."
Fukuda T., Pratto F., Schimenti J.C., Turner J.M., Camerini-Otero R.D., Hoeoeg C.
PLoS Genet. 8:E1002485-E1002485(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y08485 mRNA. Translation: CAA69719.1.
Y08486 Genomic DNA. Translation: CAA69720.1.
AF181478 expand/collapse EMBL AC list , AF181473, AF181474, AF181475, AF181476, AF181477 Genomic DNA. Translation: AAG17538.1.
AK131949 mRNA. Translation: BAE20894.1.
RefSeqNP_035647.2. NM_011517.2.
UniGeneMm.297977.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203598. 2 interactions.
IntActP70281. 3 interactions.
MINTMINT-8144355.

PTM databases

PhosphoSiteP70281.

Proteomic databases

PaxDbP70281.
PRIDEP70281.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20962.
KEGGmmu:20962.

Organism-specific databases

CTD50511.
MGIMGI:109542. Sycp3.

Phylogenomic databases

eggNOGNOG27405.
HOGENOMHOG000236293.
HOVERGENHBG100390.
InParanoidP70281.
PhylomeDBP70281.

Enzyme and pathway databases

ReactomeREACT_188804. Cell Cycle.
REACT_198624. Meiosis.
REACT_75800. Meiotic Synapsis.

Gene expression databases

CleanExMM_SYCP3.
GenevestigatorP70281.

Family and domain databases

InterProIPR006888. Cor1/Xlr/Xmr.
[Graphical view]
PANTHERPTHR19368. PTHR19368. 1 hit.
PfamPF04803. Cor1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSYCP3. mouse.
NextBio299914.
PROP70281.
SOURCESearch...

Entry information

Entry nameSYCP3_MOUSE
AccessionPrimary (citable) accession number: P70281
Secondary accession number(s): Q3V2A4, Q549A7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot