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P70280

- VAMP7_MOUSE

UniProt

P70280 - VAMP7_MOUSE

Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.1 Publication

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: UniProtKB
    2. endosome to lysosome transport Source: UniProtKB
    3. eosinophil degranulation Source: UniProtKB
    4. ER to Golgi vesicle-mediated transport Source: UniProtKB
    5. Golgi to plasma membrane protein transport Source: MGI
    6. neutrophil degranulation Source: UniProtKB
    7. phagocytosis, engulfment Source: UniProtKB
    8. regulation of protein targeting to vacuolar membrane Source: MGI
    9. SNARE complex assembly Source: BHF-UCL
    10. triglyceride transport Source: Ensembl
    11. vesicle fusion Source: UniProtKB
    12. vesicle fusion with Golgi apparatus Source: Ensembl
    13. vesicle-mediated transport Source: UniProtKB

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-associated membrane protein 7
    Short name:
    VAMP-7
    Alternative name(s):
    Synaptobrevin-like protein 1
    Gene namesi
    Name:Vamp7
    Synonyms:Sybl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1096399. Vamp7.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatustrans-Golgi network membrane By similarity; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type IV membrane protein By similarity. Cytoplasmic vesiclephagosome membrane; Single-pass type IV membrane protein. Cell junctionsynapsesynaptosome
    Note: In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney By similarity.By similarity

    GO - Cellular componenti

    1. apical part of cell Source: MGI
    2. cell junction Source: UniProtKB-KW
    3. cell surface Source: BHF-UCL
    4. endoplasmic reticulum membrane Source: UniProtKB
    5. Golgi apparatus Source: MGI
    6. integral component of membrane Source: UniProtKB-KW
    7. late endosome membrane Source: UniProtKB
    8. lysosomal membrane Source: UniProtKB
    9. neuron projection Source: UniProtKB
    10. perinuclear region of cytoplasm Source: MGI
    11. phagocytic vesicle Source: UniProtKB
    12. phagocytic vesicle membrane Source: UniProtKB-SubCell
    13. SNARE complex Source: UniProtKB
    14. synapse Source: UniProtKB-KW
    15. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 220219Vesicle-associated membrane protein 7PRO_0000316087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei168 – 1681Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP70280.
    PaxDbiP70280.
    PRIDEiP70280.

    PTM databases

    PhosphoSiteiP70280.

    Expressioni

    Gene expression databases

    BgeeiP70280.
    CleanExiMM_VAMP7.
    GenevestigatoriP70280.

    Interactioni

    Subunit structurei

    Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes By similarity. May interact with STX17.By similarity

    Protein-protein interaction databases

    BioGridi203595. 1 interaction.
    DIPiDIP-46794N.
    IntActiP70280. 3 interactions.
    MINTiMINT-1870218.
    STRINGi10090.ENSMUSP00000052262.

    Structurei

    Secondary structure

    1
    220
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Beta strandi13 – 2311
    Helixi25 – 339
    Beta strandi38 – 4710
    Beta strandi50 – 578
    Beta strandi60 – 678
    Helixi72 – 9019
    Helixi91 – 955
    Turni99 – 1024
    Helixi103 – 11816
    Helixi130 – 1345
    Turni135 – 1384
    Beta strandi151 – 1533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VX8X-ray2.20A/B/C/D1-120[»]
    4AFIX-ray2.80A/B1-120[»]
    4B93X-ray2.00A1-187[»]
    ProteinModelPortaliP70280.
    SMRiP70280. Positions 1-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 188187CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini210 – 22011VesicularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei189 – 20921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 110104LonginPROSITE-ProRule annotationAdd
    BLAST
    Domaini125 – 18561v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi191 – 1955Poly-Ile

    Sequence similaritiesi

    Belongs to the synaptobrevin family.Curated
    Contains 1 longin domain.PROSITE-ProRule annotation
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5143.
    GeneTreeiENSGT00510000047733.
    HOGENOMiHOG000042711.
    HOVERGENiHBG006675.
    InParanoidiP70280.
    KOiK08515.
    OMAiMKHYSES.
    OrthoDBiEOG75B87Z.
    PhylomeDBiP70280.
    TreeFamiTF323448.

    Family and domain databases

    Gene3Di3.30.450.50. 1 hit.
    InterProiIPR011012. Longin-like_dom.
    IPR010908. Longin_dom.
    IPR001388. Synaptobrevin.
    [Graphical view]
    PfamiPF13774. Longin. 1 hit.
    PF00957. Synaptobrevin. 1 hit.
    [Graphical view]
    PRINTSiPR00219. SYNAPTOBREVN.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS50859. LONGIN. 1 hit.
    PS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70280-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY    50
    LFHYICQDRI VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY 100
    AMNSEFSSVL AAQLKHHSEN KSLDKVMETQ AQVDELKGIM VRNIDLVAQR 150
    GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCMKNIKLT IIIIIVSIVF 200
    IYIIVSLLCG GFTWPNCVKK 220
    Length:220
    Mass (Da):24,967
    Last modified:February 1, 1997 - v1
    Checksum:i109DBA4BE5772B0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96737 mRNA. Translation: CAA65509.1.
    AJ133536
    , AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
    AK002825 mRNA. Translation: BAB22386.1.
    AK011510 mRNA. Translation: BAB27667.1.
    AK089035 mRNA. Translation: BAC40712.1.
    AK165299 mRNA. Translation: BAE38126.1.
    BC003764 mRNA. Translation: AAH03764.1.
    RefSeqiNP_035645.1. NM_011515.4.
    UniGeneiMm.1517.

    Genome annotation databases

    EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
    GeneIDi20955.
    KEGGimmu:20955.
    UCSCiuc009uym.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96737 mRNA. Translation: CAA65509.1 .
    AJ133536
    , AJ133537 , AJ133538 , AJ133539 , AJ133540 , AJ133541 , AJ133542 Genomic DNA. Translation: CAB94231.1 .
    AK002825 mRNA. Translation: BAB22386.1 .
    AK011510 mRNA. Translation: BAB27667.1 .
    AK089035 mRNA. Translation: BAC40712.1 .
    AK165299 mRNA. Translation: BAE38126.1 .
    BC003764 mRNA. Translation: AAH03764.1 .
    RefSeqi NP_035645.1. NM_011515.4.
    UniGenei Mm.1517.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VX8 X-ray 2.20 A/B/C/D 1-120 [» ]
    4AFI X-ray 2.80 A/B 1-120 [» ]
    4B93 X-ray 2.00 A 1-187 [» ]
    ProteinModelPortali P70280.
    SMRi P70280. Positions 1-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203595. 1 interaction.
    DIPi DIP-46794N.
    IntActi P70280. 3 interactions.
    MINTi MINT-1870218.
    STRINGi 10090.ENSMUSP00000052262.

    PTM databases

    PhosphoSitei P70280.

    Proteomic databases

    MaxQBi P70280.
    PaxDbi P70280.
    PRIDEi P70280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000051100 ; ENSMUSP00000052262 ; ENSMUSG00000051412 .
    GeneIDi 20955.
    KEGGi mmu:20955.
    UCSCi uc009uym.1. mouse.

    Organism-specific databases

    CTDi 6845.
    MGIi MGI:1096399. Vamp7.

    Phylogenomic databases

    eggNOGi COG5143.
    GeneTreei ENSGT00510000047733.
    HOGENOMi HOG000042711.
    HOVERGENi HBG006675.
    InParanoidi P70280.
    KOi K08515.
    OMAi MKHYSES.
    OrthoDBi EOG75B87Z.
    PhylomeDBi P70280.
    TreeFami TF323448.

    Miscellaneous databases

    NextBioi 299904.
    PROi P70280.
    SOURCEi Search...

    Gene expression databases

    Bgeei P70280.
    CleanExi MM_VAMP7.
    Genevestigatori P70280.

    Family and domain databases

    Gene3Di 3.30.450.50. 1 hit.
    InterProi IPR011012. Longin-like_dom.
    IPR010908. Longin_dom.
    IPR001388. Synaptobrevin.
    [Graphical view ]
    Pfami PF13774. Longin. 1 hit.
    PF00957. Synaptobrevin. 1 hit.
    [Graphical view ]
    PRINTSi PR00219. SYNAPTOBREVN.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS50859. LONGIN. 1 hit.
    PS00417. SYNAPTOBREVIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression pattern of XqPAR-linked genes SYBL1 and IL9R correlates with the structure and evolution of the region."
      D'Esposito M., Matarazzo M.R., Ciccodicola A., Strazzullo M., Mazzarella R., Quaderi N.A., Fujiwara H., Ko M.S., Rowe L.B., Ricco A., Archidiacono N., Rocchi M., Schlessinger D., D'Urso M.
      Hum. Mol. Genet. 6:1917-1923(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Embryo, Kidney, Spleen and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages."
      Braun V., Fraisier V., Raposo G., Hurbain I., Sibarita J.-B., Chavrier P., Galli T., Niedergang F.
      EMBO J. 23:4166-4176(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
      Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
      Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes."
      Itakura E., Kishi-Itakura C., Mizushima N.
      Cell 151:1256-1269(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STX17.

    Entry informationi

    Entry nameiVAMP7_MOUSE
    AccessioniPrimary (citable) accession number: P70280
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3