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P70280 (VAMP7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-associated membrane protein 7

Short name=VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene names
Name:Vamp7
Synonyms:Sybl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Ref.5

Subunit structure

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes By similarity. May interact with STX17.

Subcellular location

Cytoplasmic vesiclesecretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatustrans-Golgi network membrane; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Cytoplasmic vesiclephagosome membrane; Single-pass type IV membrane protein. Cell junctionsynapsesynaptosome. Note: In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney By similarity. Ref.5 Ref.7

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.

Sequence similarities

Belongs to the synaptobrevin family.

Contains 1 longin domain.

Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Biological processExocytosis
Protein transport
Transport
   Cellular componentCell junction
Cytoplasmic vesicle
Endoplasmic reticulum
Endosome
Golgi apparatus
Lysosome
Membrane
Synapse
Synaptosome
   DomainCoiled coil
Signal-anchor
Transmembrane
Transmembrane helix
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processER to Golgi vesicle-mediated transport

Inferred from sequence or structural similarity PubMed 16495485. Source: UniProtKB

Golgi to plasma membrane protein transport

Inferred from mutant phenotype PubMed 20582536. Source: MGI

SNARE complex assembly

Inferred from direct assay PubMed 21151919. Source: BHF-UCL

calcium ion-dependent exocytosis

Inferred from sequence or structural similarity PubMed 14993220. Source: UniProtKB

endocytosis

Inferred from Biological aspect of Ancestor. Source: RefGenome

endosome to lysosome transport

Inferred from sequence or structural similarity PubMed 10888671. Source: UniProtKB

eosinophil degranulation

Inferred from sequence or structural similarity PubMed 14993220. Source: UniProtKB

exocytosis

Inferred from Biological aspect of Ancestor. Source: RefGenome

neutrophil degranulation

Inferred from sequence or structural similarity PubMed 14993220. Source: UniProtKB

phagocytosis, engulfment

Inferred from sequence or structural similarity PubMed 15133481. Source: UniProtKB

regulation of protein targeting to vacuolar membrane

Inferred from mutant phenotype PubMed 18227281. Source: MGI

triglyceride transport

Inferred from electronic annotation. Source: Ensembl

vesicle fusion

Inferred from sequence or structural similarity PubMed 10888671. Source: UniProtKB

vesicle fusion with Golgi apparatus

Inferred from electronic annotation. Source: Ensembl

vesicle-mediated transport

Inferred from sequence or structural similarity PubMed 10888671. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 18321981. Source: MGI

SNARE complex

Inferred from sequence or structural similarity PubMed 15133481. Source: UniProtKB

apical part of cell

Inferred from direct assay PubMed 14758363. Source: MGI

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from direct assay PubMed 21151919. Source: BHF-UCL

endoplasmic reticulum membrane

Inferred from sequence or structural similarity PubMed 16495485. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

late endosome membrane

Inferred from sequence or structural similarity PubMed 10459012. Source: UniProtKB

lysosomal membrane

Inferred from sequence or structural similarity PubMed 10459012. Source: UniProtKB

neuron projection

Inferred from direct assay Ref.7. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from direct assay PubMed 18321981. Source: MGI

phagocytic vesicle

Inferred from direct assay Ref.5. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

synapse

Inferred from electronic annotation. Source: UniProtKB-KW

transport vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionSNAP receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

SNARE binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 220219Vesicle-associated membrane protein 7
PRO_0000316087

Regions

Topological domain2 – 188187Cytoplasmic Potential
Transmembrane189 – 20921Helical; Anchor for type IV membrane protein; Potential
Topological domain210 – 22011Vesicular Potential
Domain7 – 110104Longin
Domain125 – 18561v-SNARE coiled-coil homology
Compositional bias191 – 1955Poly-Ile

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1681Phosphoserine Ref.6

Secondary structure

........................ 220
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P70280 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 109DBA4BE5772B0C

FASTA22024,967
        10         20         30         40         50         60 
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY LFHYICQDRI 

        70         80         90        100        110        120 
VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY AMNSEFSSVL AAQLKHHSEN 

       130        140        150        160        170        180 
KSLDKVMETQ AQVDELKGIM VRNIDLVAQR GERLELLIDK TENLVDSSVT FKTTSRNLAR 

       190        200        210        220 
AMCMKNIKLT IIIIIVSIVF IYIIVSLLCG GFTWPNCVKK 

« Hide

References

« Hide 'large scale' references
[1]"Differential expression pattern of XqPAR-linked genes SYBL1 and IL9R correlates with the structure and evolution of the region."
D'Esposito M., Matarazzo M.R., Ciccodicola A., Strazzullo M., Mazzarella R., Quaderi N.A., Fujiwara H., Ko M.S., Rowe L.B., Ricco A., Archidiacono N., Rocchi M., Schlessinger D., D'Urso M.
Hum. Mol. Genet. 6:1917-1923(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Human and mouse SYBL1 gene structure and expression."
Matarazzo M.R., Cuccurese M., Strazzullo M., Vacca M., Curci A., Miano M.G., Cocchia M., Mercadante G., Torino A., D'Urso M., Ciccodicola A., D'Esposito M.
Gene 240:233-238(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Embryo, Kidney, Spleen and Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages."
Braun V., Fraisier V., Raposo G., Hurbain I., Sibarita J.-B., Chavrier P., Galli T., Niedergang F.
EMBO J. 23:4166-4176(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes."
Itakura E., Kishi-Itakura C., Mizushima N.
Cell 151:1256-1269(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STX17.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96737 mRNA. Translation: CAA65509.1.
AJ133536 expand/collapse EMBL AC list , AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
AK002825 mRNA. Translation: BAB22386.1.
AK011510 mRNA. Translation: BAB27667.1.
AK089035 mRNA. Translation: BAC40712.1.
AK165299 mRNA. Translation: BAE38126.1.
BC003764 mRNA. Translation: AAH03764.1.
RefSeqNP_035645.1. NM_011515.4.
UniGeneMm.1517.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortalP70280.
SMRP70280. Positions 1-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203595. 1 interaction.
DIPDIP-46794N.
IntActP70280. 3 interactions.
MINTMINT-1870218.
STRING10090.ENSMUSP00000052262.

PTM databases

PhosphoSiteP70280.

Proteomic databases

MaxQBP70280.
PaxDbP70280.
PRIDEP70280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
GeneID20955.
KEGGmmu:20955.
UCSCuc009uym.1. mouse.

Organism-specific databases

CTD6845.
MGIMGI:1096399. Vamp7.

Phylogenomic databases

eggNOGCOG5143.
GeneTreeENSGT00510000047733.
HOGENOMHOG000042711.
HOVERGENHBG006675.
InParanoidP70280.
KOK08515.
OMAMKHYSES.
OrthoDBEOG75B87Z.
PhylomeDBP70280.
TreeFamTF323448.

Gene expression databases

BgeeP70280.
CleanExMM_VAMP7.
GenevestigatorP70280.

Family and domain databases

Gene3D3.30.450.50. 1 hit.
InterProIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSPR00219. SYNAPTOBREVN.
SUPFAMSSF64356. SSF64356. 1 hit.
PROSITEPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio299904.
PROP70280.
SOURCESearch...

Entry information

Entry nameVAMP7_MOUSE
AccessionPrimary (citable) accession number: P70280
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot