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Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.1 Publication

GO - Molecular functioni

  1. SNAP receptor activity Source: GO_Central
  2. SNARE binding Source: GO_Central

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: UniProtKB
  2. endocytosis Source: GO_Central
  3. endosome to lysosome transport Source: UniProtKB
  4. eosinophil degranulation Source: UniProtKB
  5. ER to Golgi vesicle-mediated transport Source: UniProtKB
  6. exocytosis Source: GO_Central
  7. Golgi to plasma membrane protein transport Source: MGI
  8. neutrophil degranulation Source: UniProtKB
  9. phagocytosis, engulfment Source: UniProtKB
  10. positive regulation of dendrite morphogenesis Source: UniProtKB
  11. regulation of protein targeting to vacuolar membrane Source: MGI
  12. SNARE complex assembly Source: BHF-UCL
  13. triglyceride transport Source: Ensembl
  14. vesicle fusion Source: UniProtKB
  15. vesicle fusion with Golgi apparatus Source: Ensembl
  16. vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_231722. Lysosome Vesicle Biogenesis.
REACT_232671. Golgi Associated Vesicle Biogenesis.
REACT_234979. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene namesi
Name:Vamp7
Synonyms:Sybl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1096399. Vamp7.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatustrans-Golgi network membrane By similarity; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type IV membrane protein By similarity. Cytoplasmic vesiclephagosome membrane; Single-pass type IV membrane protein. Cell junctionsynapsesynaptosome
Note: In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 188187CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei189 – 20921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini210 – 22011VesicularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. cell surface Source: BHF-UCL
  4. cytoplasm Source: MGI
  5. endoplasmic reticulum membrane Source: UniProtKB
  6. extracellular vesicular exosome Source: MGI
  7. Golgi apparatus Source: MGI
  8. integral component of membrane Source: UniProtKB-KW
  9. intracellular membrane-bounded organelle Source: MGI
  10. lamellipodium Source: UniProtKB
  11. late endosome membrane Source: UniProtKB
  12. lysosomal membrane Source: UniProtKB
  13. neuron projection Source: UniProtKB
  14. perinuclear region of cytoplasm Source: MGI
  15. phagocytic vesicle Source: UniProtKB
  16. phagocytic vesicle membrane Source: UniProtKB-SubCell
  17. pseudopodium Source: UniProtKB
  18. SNARE complex Source: UniProtKB
  19. synapse Source: UniProtKB-KW
  20. transport vesicle Source: UniProtKB
  21. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 220219Vesicle-associated membrane protein 7PRO_0000316087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei168 – 1681Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP70280.
PaxDbiP70280.
PRIDEiP70280.

PTM databases

PhosphoSiteiP70280.

Expressioni

Gene expression databases

BgeeiP70280.
CleanExiMM_VAMP7.
GenevestigatoriP70280.

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17.By similarity

Protein-protein interaction databases

BioGridi203595. 1 interaction.
DIPiDIP-46794N.
IntActiP70280. 3 interactions.
MINTiMINT-1870218.
STRINGi10090.ENSMUSP00000052262.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109Combined sources
Beta strandi13 – 2311Combined sources
Helixi25 – 339Combined sources
Beta strandi38 – 4710Combined sources
Beta strandi50 – 578Combined sources
Beta strandi60 – 678Combined sources
Helixi72 – 9019Combined sources
Helixi91 – 955Combined sources
Turni99 – 1024Combined sources
Helixi103 – 11816Combined sources
Helixi130 – 1345Combined sources
Turni135 – 1384Combined sources
Beta strandi151 – 1533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortaliP70280.
SMRiP70280. Positions 1-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 110104LonginPROSITE-ProRule annotationAdd
BLAST
Domaini125 – 18561v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi191 – 1955Poly-Ile

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP70280.
KOiK08515.
OMAiCHDRIIY.
OrthoDBiEOG75B87Z.
PhylomeDBiP70280.
TreeFamiTF323448.

Family and domain databases

Gene3Di3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70280-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN KSLDKVMETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCMKNIKLT IIIIIVSIVF
210 220
IYIIVSLLCG GFTWPNCVKK
Length:220
Mass (Da):24,967
Last modified:February 1, 1997 - v1
Checksum:i109DBA4BE5772B0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96737 mRNA. Translation: CAA65509.1.
AJ133536
, AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
AK002825 mRNA. Translation: BAB22386.1.
AK011510 mRNA. Translation: BAB27667.1.
AK089035 mRNA. Translation: BAC40712.1.
AK165299 mRNA. Translation: BAE38126.1.
BC003764 mRNA. Translation: AAH03764.1.
RefSeqiNP_001289067.1. NM_001302138.1.
NP_035645.1. NM_011515.5.
UniGeneiMm.1517.

Genome annotation databases

EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
GeneIDi20955.
KEGGimmu:20955.
UCSCiuc009uym.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96737 mRNA. Translation: CAA65509.1.
AJ133536
, AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
AK002825 mRNA. Translation: BAB22386.1.
AK011510 mRNA. Translation: BAB27667.1.
AK089035 mRNA. Translation: BAC40712.1.
AK165299 mRNA. Translation: BAE38126.1.
BC003764 mRNA. Translation: AAH03764.1.
RefSeqiNP_001289067.1. NM_001302138.1.
NP_035645.1. NM_011515.5.
UniGeneiMm.1517.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortaliP70280.
SMRiP70280. Positions 1-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203595. 1 interaction.
DIPiDIP-46794N.
IntActiP70280. 3 interactions.
MINTiMINT-1870218.
STRINGi10090.ENSMUSP00000052262.

PTM databases

PhosphoSiteiP70280.

Proteomic databases

MaxQBiP70280.
PaxDbiP70280.
PRIDEiP70280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
GeneIDi20955.
KEGGimmu:20955.
UCSCiuc009uym.1. mouse.

Organism-specific databases

CTDi6845.
MGIiMGI:1096399. Vamp7.

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP70280.
KOiK08515.
OMAiCHDRIIY.
OrthoDBiEOG75B87Z.
PhylomeDBiP70280.
TreeFamiTF323448.

Enzyme and pathway databases

ReactomeiREACT_231722. Lysosome Vesicle Biogenesis.
REACT_232671. Golgi Associated Vesicle Biogenesis.
REACT_234979. Clathrin derived vesicle budding.

Miscellaneous databases

NextBioi299904.
PROiP70280.
SOURCEiSearch...

Gene expression databases

BgeeiP70280.
CleanExiMM_VAMP7.
GenevestigatoriP70280.

Family and domain databases

Gene3Di3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression pattern of XqPAR-linked genes SYBL1 and IL9R correlates with the structure and evolution of the region."
    D'Esposito M., Matarazzo M.R., Ciccodicola A., Strazzullo M., Mazzarella R., Quaderi N.A., Fujiwara H., Ko M.S., Rowe L.B., Ricco A., Archidiacono N., Rocchi M., Schlessinger D., D'Urso M.
    Hum. Mol. Genet. 6:1917-1923(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Kidney, Spleen and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages."
    Braun V., Fraisier V., Raposo G., Hurbain I., Sibarita J.-B., Chavrier P., Galli T., Niedergang F.
    EMBO J. 23:4166-4176(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
    Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
    Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes."
    Itakura E., Kishi-Itakura C., Mizushima N.
    Cell 151:1256-1269(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STX17.

Entry informationi

Entry nameiVAMP7_MOUSE
AccessioniPrimary (citable) accession number: P70280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.