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Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.1 Publication

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.

GO - Molecular functioni

GO - Biological processi

  • calcium ion regulated exocytosis Source: UniProtKB
  • endocytosis Source: GO_Central
  • endosome to lysosome transport Source: UniProtKB
  • eosinophil degranulation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: GO_Central
  • Golgi to plasma membrane protein transport Source: MGI
  • natural killer cell degranulation Source: MGI
  • neutrophil degranulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: UniProtKB
  • positive regulation of histamine secretion by mast cell Source: MGI
  • regulation of protein targeting to vacuolar membrane Source: MGI
  • SNARE complex assembly Source: BHF-UCL
  • triglyceride transport Source: Ensembl
  • vesicle fusion Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: Ensembl
  • vesicle-mediated transport Source: UniProtKB
  • vesicle transport along microtubule Source: Ensembl

Keywordsi

Biological processExocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-421837 Clathrin derived vesicle budding
R-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-9020591 Interleukin-12 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene namesi
Name:Vamp7
Synonyms:Sybl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1096399 Vamp7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 188CytoplasmicSequence analysisAdd BLAST187
Transmembranei189 – 209Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini210 – 220VesicularSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003160872 – 220Vesicle-associated membrane protein 7Add BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei168PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70280
MaxQBiP70280
PaxDbiP70280
PeptideAtlasiP70280
PRIDEiP70280

PTM databases

iPTMnetiP70280
PhosphoSitePlusiP70280
SwissPalmiP70280

Expressioni

Gene expression databases

BgeeiENSMUSG00000051412
CleanExiMM_VAMP7
GenevisibleiP70280 MM

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203595, 1 interactor
CORUMiP70280
DIPiDIP-46794N
IntActiP70280, 12 interactors
MINTiP70280
STRINGi10090.ENSMUSP00000052262

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi13 – 23Combined sources11
Helixi25 – 33Combined sources9
Beta strandi38 – 47Combined sources10
Beta strandi50 – 57Combined sources8
Beta strandi60 – 67Combined sources8
Helixi72 – 90Combined sources19
Helixi91 – 95Combined sources5
Turni99 – 102Combined sources4
Helixi103 – 118Combined sources16
Helixi130 – 134Combined sources5
Turni135 – 138Combined sources4
Beta strandi151 – 153Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortaliP70280
SMRiP70280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 110LonginPROSITE-ProRule annotationAdd BLAST104
Domaini125 – 185v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 195Poly-Ile5

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0859 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00510000047733
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiP70280
KOiK08515
OMAiKLTYTHG
OrthoDBiEOG091G0MPE
PhylomeDBiP70280
TreeFamiTF323448

Family and domain databases

InterProiView protein in InterPro
IPR011012 Longin-like_dom_sf
IPR010908 Longin_dom
IPR001388 Synaptobrevin
PfamiView protein in Pfam
PF13774 Longin, 1 hit
PF00957 Synaptobrevin, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
SMARTiView protein in SMART
SM01270 Longin, 1 hit
SUPFAMiSSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS50859 LONGIN, 1 hit
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN KSLDKVMETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCMKNIKLT IIIIIVSIVF
210 220
IYIIVSLLCG GFTWPNCVKK
Length:220
Mass (Da):24,967
Last modified:February 1, 1997 - v1
Checksum:i109DBA4BE5772B0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96737 mRNA Translation: CAA65509.1
AJ133536
, AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA Translation: CAB94231.1
AK002825 mRNA Translation: BAB22386.1
AK011510 mRNA Translation: BAB27667.1
AK089035 mRNA Translation: BAC40712.1
AK165299 mRNA Translation: BAE38126.1
BC003764 mRNA Translation: AAH03764.1
RefSeqiNP_001289067.1, NM_001302138.1
NP_035645.1, NM_011515.5
UniGeneiMm.1517

Genome annotation databases

EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412
GeneIDi20955
KEGGimmu:20955
UCSCiuc009uym.2 mouse

Similar proteinsi

Entry informationi

Entry nameiVAMP7_MOUSE
AccessioniPrimary (citable) accession number: P70280
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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