Reviewed, UniProtKB/Swiss-Prot P70280 (VAMP7_MOUSE)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 7
      Short name=VAMP-7
Alternative name(s):
    Synaptobrevin-like protein 1

Gene names

Name:	Vamp7
Synonyms:	Sybl1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Ref.5
Subunit structure	Component of the SNARE complex composed of Stx4, Snap23 and Vamp7 that binds Syt7 during lysosomal exocytosis. Component of the SNARE complex composed of Stx7, Stx8, Vamp7 and Vti1b that is required for heterotypic fusion of late endosomes with lysosomes in liver cells By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Cytoplasmic vesicle › phagosome membrane; Single-pass type IV membrane protein.
Miscellaneous	Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Lysosome Membrane
Domain	Coiled coil Signal-anchor Transmembrane
PTM	Acetylation
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB calcium ion-dependent exocytosis Inferred from sequence or structural similarity. Source: UniProtKB endosome to lysosome transport Inferred from sequence or structural similarity. Source: UniProtKB eosinophil degranulation Inferred from sequence or structural similarity. Source: UniProtKB neutrophil degranulation Inferred from sequence or structural similarity. Source: UniProtKB phagocytosis, engulfment Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle fusion Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW SNARE complex Inferred from sequence or structural similarity. Source: UniProtKB apical part of cell Inferred from direct assay. Source: MGI endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome membrane Inferred from sequence or structural similarity. Source: UniProtKB lysosomal membrane Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle Ref.5 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 220	219	Vesicle-associated membrane protein 7		PRO_0000316087
Regions
	Topological domain	2 – 188	187	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Anchor for type IV membrane protein Potential
	Topological domain	210 – 220	11	Vesicular Potential
	Domain	7 – 110	104	Longin
	Domain	125 – 185	61	v-SNARE coiled-coil homology
	Compositional bias	191 – 195	5	Poly-Ile
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70280-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 109DBA4BE5772B0C	FASTA	220	24,967
	10 20 30 40 50 60 MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY LFHYICQDRI 70 80 90 100 110 120 VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY AMNSEFSSVL AAQLKHHSEN 130 140 150 160 170 180 KSLDKVMETQ AQVDELKGIM VRNIDLVAQR GERLELLIDK TENLVDSSVT FKTTSRNLAR 190 200 210 220 AMCMKNIKLT IIIIIVSIVF IYIIVSLLCG GFTWPNCVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression pattern of XqPAR-linked genes SYBL1 and IL9R correlates with the structure and evolution of the region." D'Esposito M., Matarazzo M.R., Ciccodicola A., Strazzullo M., Mazzarella R., Quaderi N.A., Fujiwara H., Ko M.S., Rowe L.B., Ricco A., Archidiacono N., Rocchi M., Schlessinger D., D'Urso M. Hum. Mol. Genet. 6:1917-1923(1997) [PubMed: 9302271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human and mouse SYBL1 gene structure and expression." Matarazzo M.R., Cuccurese M., Strazzullo M., Vacca M., Curci A., Miano M.G., Cocchia M., Mercadante G., Torino A., D'Urso M., Ciccodicola A., D'Esposito M. Gene 240:233-238(1999) [PubMed: 10564831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Embryo, Kidney, Spleen and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages." Braun V., Fraisier V., Raposo G., Hurbain I., Sibarita J.-B., Chavrier P., Galli T., Niedergang F. EMBO J. 23:4166-4176(2004) [PubMed: 15470500] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96737 mRNA. Translation: CAA65509.1. AJ133536 , AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1. AK002825 mRNA. Translation: BAB22386.1. AK011510 mRNA. Translation: BAB27667.1. AK089035 mRNA. Translation: BAC40712.1. AK165299 mRNA. Translation: BAE38126.1. BC003764 mRNA. Translation: AAH03764.1.
IPI	IPI00137647.
RefSeq	NP_035645.1.
UniGene	Mm.1517
3D structure databases
HSSP	HSSP built from PDB template 1GL2 based on UniProtKB Q9WUF4.
SMR	P70280. Positions 1-122.
ModBase	Search...
Protein-protein interaction databases
STRING	P70280.
PTM databases
PhosphoSite	P70280.
Proteomic databases
PRIDE	P70280.
Genome annotation databases
Ensembl	ENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412; Mus musculus. [Genome view]
GeneID	20955.
KEGG	mmu:20955.
UCSC	uc009uym.1. mouse.
Organism-specific databases
CTD	20955.
MGI	MGI:1096399. Vamp7.
Phylogenomic databases
HOGENOM	P70280.
HOVERGEN	P70280.
OMA	FLCMAND.
Gene expression databases
ArrayExpress	P70280.
Bgee	P70280.
CleanEx	MM_VAMP7.
Genevestigator	P70280.
Family and domain databases
InterPro	IPR010908. Longin. IPR001388. Synaptobrevin. [Graphical view]
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PRINTS	PR00219. SYNAPTOBREVN.
ProDom	PD001229. Synaptobrevin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50859. LONGIN. 1 hit. PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	299904.
SOURCE	Search...

Entry information

Entry name

VAMP7_MOUSE

Accession

Primary (citable) accession number: P70280

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)