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Protein

Vesicle-associated membrane protein 7

Gene

Vamp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.1 Publication

GO - Molecular functioni

GO - Biological processi

  • calcium ion regulated exocytosis Source: UniProtKB
  • endocytosis Source: GO_Central
  • endosome to lysosome transport Source: UniProtKB
  • eosinophil degranulation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: GO_Central
  • Golgi to plasma membrane protein transport Source: MGI
  • natural killer cell degranulation Source: MGI
  • neutrophil degranulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: UniProtKB
  • positive regulation of histamine secretion by mast cell Source: MGI
  • regulation of protein targeting to vacuolar membrane Source: MGI
  • SNARE complex assembly Source: BHF-UCL
  • triglyceride transport Source: Ensembl
  • vesicle fusion Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: Ensembl
  • vesicle-mediated transport Source: UniProtKB
  • vesicle transport along microtubule Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-421837. Clathrin derived vesicle budding.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Gene namesi
Name:Vamp7
Synonyms:Sybl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1096399. Vamp7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 188CytoplasmicSequence analysisAdd BLAST187
Transmembranei189 – 209Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini210 – 220VesicularSequence analysisAdd BLAST11

GO - Cellular componenti

  • apical part of cell Source: MGI
  • azurophil granule membrane Source: MGI
  • cell junction Source: UniProtKB-KW
  • cell surface Source: BHF-UCL
  • cytoplasm Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: MGI
  • lamellipodium Source: UniProtKB
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • membrane Source: MGI
  • neuron projection Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
  • platelet alpha granule Source: MGI
  • pseudopodium Source: UniProtKB
  • secretory granule Source: MGI
  • secretory granule membrane Source: MGI
  • SNARE complex Source: UniProtKB
  • synapse Source: UniProtKB-KW
  • trans-Golgi network Source: MGI
  • transport vesicle Source: UniProtKB
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003160872 – 220Vesicle-associated membrane protein 7Add BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei168PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70280.
MaxQBiP70280.
PaxDbiP70280.
PeptideAtlasiP70280.
PRIDEiP70280.

PTM databases

iPTMnetiP70280.
PhosphoSitePlusiP70280.
SwissPalmiP70280.

Expressioni

Gene expression databases

BgeeiENSMUSG00000051412.
CleanExiMM_VAMP7.
GenevisibleiP70280. MM.

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
STX17P569622EBI-6555653,EBI-2797775From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203595. 1 interactor.
DIPiDIP-46794N.
IntActiP70280. 5 interactors.
MINTiMINT-1870218.
STRINGi10090.ENSMUSP00000052262.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Beta strandi13 – 23Combined sources11
Helixi25 – 33Combined sources9
Beta strandi38 – 47Combined sources10
Beta strandi50 – 57Combined sources8
Beta strandi60 – 67Combined sources8
Helixi72 – 90Combined sources19
Helixi91 – 95Combined sources5
Turni99 – 102Combined sources4
Helixi103 – 118Combined sources16
Helixi130 – 134Combined sources5
Turni135 – 138Combined sources4
Beta strandi151 – 153Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortaliP70280.
SMRiP70280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 110LonginPROSITE-ProRule annotationAdd BLAST104
Domaini125 – 185v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 195Poly-Ile5

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0859. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP70280.
KOiK08515.
OMAiARSLFWK.
OrthoDBiEOG091G0MPE.
PhylomeDBiP70280.
TreeFamiTF323448.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFSFLN EVKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN KSLDKVMETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCMKNIKLT IIIIIVSIVF
210 220
IYIIVSLLCG GFTWPNCVKK
Length:220
Mass (Da):24,967
Last modified:February 1, 1997 - v1
Checksum:i109DBA4BE5772B0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96737 mRNA. Translation: CAA65509.1.
AJ133536
, AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
AK002825 mRNA. Translation: BAB22386.1.
AK011510 mRNA. Translation: BAB27667.1.
AK089035 mRNA. Translation: BAC40712.1.
AK165299 mRNA. Translation: BAE38126.1.
BC003764 mRNA. Translation: AAH03764.1.
RefSeqiNP_001289067.1. NM_001302138.1.
NP_035645.1. NM_011515.5.
UniGeneiMm.1517.

Genome annotation databases

EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
GeneIDi20955.
KEGGimmu:20955.
UCSCiuc009uym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96737 mRNA. Translation: CAA65509.1.
AJ133536
, AJ133537, AJ133538, AJ133539, AJ133540, AJ133541, AJ133542 Genomic DNA. Translation: CAB94231.1.
AK002825 mRNA. Translation: BAB22386.1.
AK011510 mRNA. Translation: BAB27667.1.
AK089035 mRNA. Translation: BAC40712.1.
AK165299 mRNA. Translation: BAE38126.1.
BC003764 mRNA. Translation: AAH03764.1.
RefSeqiNP_001289067.1. NM_001302138.1.
NP_035645.1. NM_011515.5.
UniGeneiMm.1517.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VX8X-ray2.20A/B/C/D1-120[»]
4AFIX-ray2.80A/B1-120[»]
4B93X-ray2.00A1-187[»]
ProteinModelPortaliP70280.
SMRiP70280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203595. 1 interactor.
DIPiDIP-46794N.
IntActiP70280. 5 interactors.
MINTiMINT-1870218.
STRINGi10090.ENSMUSP00000052262.

PTM databases

iPTMnetiP70280.
PhosphoSitePlusiP70280.
SwissPalmiP70280.

Proteomic databases

EPDiP70280.
MaxQBiP70280.
PaxDbiP70280.
PeptideAtlasiP70280.
PRIDEiP70280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051100; ENSMUSP00000052262; ENSMUSG00000051412.
GeneIDi20955.
KEGGimmu:20955.
UCSCiuc009uym.2. mouse.

Organism-specific databases

CTDi6845.
MGIiMGI:1096399. Vamp7.

Phylogenomic databases

eggNOGiKOG0859. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP70280.
KOiK08515.
OMAiARSLFWK.
OrthoDBiEOG091G0MPE.
PhylomeDBiP70280.
TreeFamiTF323448.

Enzyme and pathway databases

ReactomeiR-MMU-421837. Clathrin derived vesicle budding.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiP70280.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051412.
CleanExiMM_VAMP7.
GenevisibleiP70280. MM.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP7_MOUSE
AccessioniPrimary (citable) accession number: P70280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired receptor-mediated phagocytosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.