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Protein

Stimulated by retinoic acid gene 8 protein

Gene

Stra8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription.6 Publications

GO - Biological processi

  • cellular response to retinoic acid Source: UniProtKB
  • DNA replication Source: UniProtKB-KW
  • female meiosis sister chromatid cohesion Source: MGI
  • fertilization Source: MGI
  • meiotic chromosome condensation Source: MGI
  • meiotic DNA double-strand break formation Source: MGI
  • meiotic DNA replication checkpoint Source: MGI
  • oocyte development Source: MGI
  • ovarian follicle development Source: MGI
  • reciprocal meiotic recombination Source: MGI
  • regulation of organ growth Source: MGI
  • spermatogenesis Source: MGI
  • synapsis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Differentiation, DNA replication, Meiosis, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Stimulated by retinoic acid gene 8 protein
Gene namesi
Name:Stra8Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:107917. Stra8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Female and male mice are infertile due to severe gametogenesis impairment. In female mutant embryos, the initial mitotic development of germ cells is normal, but they fail to undergo promeiotic DNA replication and meiotic chromosome condensation. In male mutants, the premeiotic DNA replication is conserved and germ cells are able to partly condense chromosomes and initiate meiotic recombination. However, they fail to regularily continue over the leptotene stage of prophase I.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Stimulated by retinoic acid gene 8 proteinPRO_0000318115Add
BLAST

Post-translational modificationi

Phosphorylated in P19 EC cells.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70278.
PRIDEiP70278.

PTM databases

iPTMnetiP70278.
PhosphoSiteiP70278.

Expressioni

Tissue specificityi

Expressed exclusively in premeiotic germ cells in both sexes. In females, is expressed in the embryonic ovary. In males, is expressed in pubertal and adult testes, in premeiotic spermatogenic cells. Expressed by some type A and B spermatogonia, preleptotene spermatocytes, and early leptotene spermatocytes (at protein level). Expression begins in late undifferntiated spermatogonia and persists during in differentiating spermatogonia (at protein level).4 Publications

Developmental stagei

Up-regulated in embryonic germ cells of the female gonads in an anterior-to-posterior wave from E12.5 to E16.5. In male gonads, expression is first detected after birth.2 Publications

Inductioni

Up-regulated by retinoic acid in embryonic ovaries and adult testes, and by all-trans and 9-cis retinoic acid in P19 embryonic carcinoma (EC) cells. Transcription is repressed by DMRT1 in undifferentiated spermatogonia.4 Publications

Gene expression databases

BgeeiP70278.
CleanExiMM_STRA8.
ExpressionAtlasiP70278. baseline and differential.
GenevisibleiP70278. MM.

Interactioni

Subunit structurei

Interacts with XPO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Arid4bA2CG634EBI-6394115,EBI-6394082

Protein-protein interaction databases

IntActiP70278. 6 interactions.
STRINGi10090.ENSMUSP00000031876.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili66 – 9530Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi28 – 336Nuclear localization signal (NLS)1 Publication
Motifi209 – 21810Nuclear export signal (NES)1 Publication

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 19391Glu-richSequence analysisAdd
BLAST
Compositional biasi377 – 3804Poly-AspSequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEEF. Eukaryota.
ENOG410ZG79. LUCA.
GeneTreeiENSGT00390000017181.
HOGENOMiHOG000154445.
HOVERGENiHBG095604.
InParanoidiP70278.
OMAiLTASKWQ.
OrthoDBiEOG7M98GS.
PhylomeDBiP70278.
TreeFamiTF335594.

Sequencei

Sequence statusi: Complete.

P70278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPGEGNQP SDDGAPQPLA QLQKLEPRVV RRRLSQARHR ATLVGLFNNL
60 70 80 90 100
RKAVYSQSDI TASKWQVLNR TKIHIQEQEE SLDKLLKLKA SFNLQDGNPN
110 120 130 140 150
SLEEVKEEYA RMYSENDSVF LNSFLQDSPP EWFPSEAVGP DAEEEGEEEG
160 170 180 190 200
EEEGEEGEEE EEGDEEGEEE EENGEEREVE EYQEEEEEEE EEEKKVDLSH
210 220 230 240 250
SSSTLLPDLM EFERYLNFYK QTMDLLTMNS IISAHEVTLP IVSAAISHLW
260 270 280 290 300
QTLSEEKKAR LLQVWEQQHS AFADLTEACL ELAGVEGSMK DSGVDSQGAS
310 320 330 340 350
CSLESTPEEI LFEDAFDVAS FLDKSEAQHM SNISAMFATC NSENPEEKFQ
360 370 380 390
LYIQIIEFFK SLGCVNTPLN QEPEPPDDDD AMLLKCLETF DDL
Length:393
Mass (Da):44,555
Last modified:February 1, 1997 - v1
Checksum:iB81DBF3D28082E9F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 6312KAVYS…DITAS → SVEGAWFTVVAL in AAI00747 (PubMed:15489334).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75287 mRNA. Translation: CAA99711.1.
BC100746 mRNA. Translation: AAI00747.1.
BC103590 mRNA. Translation: AAI03591.1.
BC103591 mRNA. Translation: AAI03592.1.
CCDSiCCDS39458.1.
RefSeqiNP_033318.1. NM_009292.1.
XP_011239572.1. XM_011241270.1.
UniGeneiMm.5171.

Genome annotation databases

EnsembliENSMUST00000031876; ENSMUSP00000031876; ENSMUSG00000029848.
ENSMUST00000114999; ENSMUSP00000110651; ENSMUSG00000029848.
GeneIDi20899.
KEGGimmu:20899.
UCSCiuc009bia.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75287 mRNA. Translation: CAA99711.1.
BC100746 mRNA. Translation: AAI00747.1.
BC103590 mRNA. Translation: AAI03591.1.
BC103591 mRNA. Translation: AAI03592.1.
CCDSiCCDS39458.1.
RefSeqiNP_033318.1. NM_009292.1.
XP_011239572.1. XM_011241270.1.
UniGeneiMm.5171.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70278. 6 interactions.
STRINGi10090.ENSMUSP00000031876.

PTM databases

iPTMnetiP70278.
PhosphoSiteiP70278.

Proteomic databases

PaxDbiP70278.
PRIDEiP70278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031876; ENSMUSP00000031876; ENSMUSG00000029848.
ENSMUST00000114999; ENSMUSP00000110651; ENSMUSG00000029848.
GeneIDi20899.
KEGGimmu:20899.
UCSCiuc009bia.1. mouse.

Organism-specific databases

CTDi346673.
MGIiMGI:107917. Stra8.

Phylogenomic databases

eggNOGiENOG410IEEF. Eukaryota.
ENOG410ZG79. LUCA.
GeneTreeiENSGT00390000017181.
HOGENOMiHOG000154445.
HOVERGENiHBG095604.
InParanoidiP70278.
OMAiLTASKWQ.
OrthoDBiEOG7M98GS.
PhylomeDBiP70278.
TreeFamiTF335594.

Miscellaneous databases

NextBioi299761.
PROiP70278.
SOURCEiSearch...

Gene expression databases

BgeeiP70278.
CleanExiMM_STRA8.
ExpressionAtlasiP70278. baseline and differential.
GenevisibleiP70278. MM.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene."
    Oulad-Abdelghani M., Bouillet P., Decimo D., Gansmuller A., Heyberger S., Dolle P., Bronner S., Lutz Y., Chambon P.
    J. Cell Biol. 135:469-477(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, PHOSPHORYLATION.
    Tissue: Embryonic carcinomaImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Sexual differentiation of germ cells in XX mouse gonads occurs in an anterior-to-posterior wave."
    Menke D.B., Koubova J., Page D.C.
    Dev. Biol. 262:303-312(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  4. "In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication."
    Baltus A.E., Menke D.B., Hu Y.-C., Goodheart M.L., Carpenter A.E., de Rooij D.G., Page D.C.
    Nat. Genet. 38:1430-1434(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Retinoic acid regulates sex-specific timing of meiotic initiation in mice."
    Koubova J., Menke D.B., Zhou Q., Capel B., Griswold M.D., Page D.C.
    Proc. Natl. Acad. Sci. U.S.A. 103:2474-2479(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, INDUCTION.
  6. "Expression of stimulated by retinoic acid gene 8 (Stra8) in spermatogenic cells induced by retinoic acid: an in vivo study in vitamin A-sufficient postnatal murine testes."
    Zhou Q., Nie R., Li Y., Friel P., Mitchell D., Hess R.A., Small C., Griswold M.D.
    Biol. Reprod. 79:35-42(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  7. "Stra8 and its inducer, retinoic acid, regulate meiotic initiation in both spermatogenesis and oogenesis in mice."
    Anderson E.L., Baltus A.E., Roepers-Gajadien H.L., Hassold T.J., de Rooij D.G., van Pelt A.M., Page D.C.
    Proc. Natl. Acad. Sci. U.S.A. 105:14976-14980(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation."
    Mark M., Jacobs H., Oulad-Abdelghani M., Dennefeld C., Feret B., Vernet N., Codreanu C.A., Chambon P., Ghyselinck N.B.
    J. Cell Sci. 121:3233-3242(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "STRA8 shuttles between nucleus and cytoplasm and displays transcriptional activity."
    Tedesco M., La Sala G., Barbagallo F., De Felici M., Farini D.
    J. Biol. Chem. 284:35781-35793(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH XPO1.
  10. "The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells."
    Matson C.K., Murphy M.W., Griswold M.D., Yoshida S., Bardwell V.J., Zarkower D.
    Dev. Cell 19:612-624(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "Periodic retinoic acid-STRA8 signaling intersects with periodic germ-cell competencies to regulate spermatogenesis."
    Endo T., Romer K.A., Anderson E.L., Baltus A.E., de Rooij D.G., Page D.C.
    Proc. Natl. Acad. Sci. U.S.A. 112:E2347-E2356(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSTRA8_MOUSE
AccessioniPrimary (citable) accession number: P70278
Secondary accession number(s): Q496Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.