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P70275 (SEM3E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Semaphorin-3E
Alternative name(s):
Semaphorin-H
Short name=Sema H
Gene names
Name:Sema3e
Synonyms:Semah, Semh
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation. Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Interacts with PLXND1. Ref.6 Ref.8

Subcellular location

Secreted.

Tissue specificity

Detected in neurons in the thalamus. Detected in embryonic vasculature. Developing lungs, developing skeletal elements and ventral horns of the developing neural tube. Correlates positively with tumor progression. Ref.6 Ref.9

Disruption phenotype

Embryos present defects in the patterning of intersomitic vasculature. Ref.6

Sequence similarities

Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Sema domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PLXND1Q9Y4D72EBI-8876322,EBI-310731From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 775750Semaphorin-3E
PRO_0000032318

Regions

Domain32 – 516485Sema
Domain581 – 66989Ig-like C2-type
Compositional bias737 – 77034Arg/Lys-rich (basic)

Amino acid modifications

Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation1261N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 115 By similarity
Disulfide bond133 ↔ 142 By similarity
Disulfide bond270 ↔ 382 By similarity
Disulfide bond294 ↔ 342 By similarity
Disulfide bond519 ↔ 537 By similarity
Disulfide bond654 ↔ 729 By similarity

Experimental info

Sequence conflict407 – 4082MH → ID in CAB02590. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P70275 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 221E766F404098D4

FASTA77589,543
        10         20         30         40         50         60 
MAPAGHILTL LLWGHLLELW TPGHSANPSY PRLRLSHKEL LELNRTSIFQ SPLGFLDLHT 

        70         80         90        100        110        120 
MLLDEYQERL FVGGRDLVYS LNLERVSDGY REIYWPSTAV KVEECIMKGK DANECANYIR 

       130        140        150        160        170        180 
VLHHYNRTHL LTCATGAFDP HCAFIRVGHH SEEPLFHLES HRSERGRGRC PFDPNSSFVS 

       190        200        210        220        230        240 
TLVGNELFAG LYSDYWGRDS AIFRSMGKLG HIRTEHDDER LLKEPKFVGS YMIPDNEDRD 

       250        260        270        280        290        300 
DNKMYFFFTE KALEAENNAH TIYTRVGRLC VNDMGGQRIL VNKWSTFLKA RLVCSVPGMN 

       310        320        330        340        350        360 
GIDTYFDELE DVFLLPTRDP KNPVIFGLFN TTSNIFRGHA VCVYHMSSIR EAFNGPYAHK 

       370        380        390        400        410        420 
EGPEYHWSLY EGKVPYPRPG SCASKVNGGK YGTTKDYPDD AIRFARMHPL MYQPIKPVHK 

       430        440        450        460        470        480 
KPILVKTDGK YNLRQLAVDR VEAEDGQYDV LFIGTDTGIV LKVITIYNQE TEWMEEVILE 

       490        500        510        520        530        540 
ELQIFKDPAP IISMEISSKR QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD 

       550        560        570        580        590        600 
GISCSRYYPT GAHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDRTEERLA YGIESNSTLL 

       610        620        630        640        650        660 
ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG TYFCQTVEHN 

       670        680        690        700        710        720 
FVHTVRKITL EVVEEHKVEG MFHKDHEEER HHKMPCPPLS GMSQGTKPWY KEFLQLIGYS 

       730        740        750        760        770 
NFQRVEEYCE KVWCTDKKRK KLKMSPSKWK YANPQEKRLR SKAEHFRLPR HTLLS 

« Hide

References

« Hide 'large scale' references
[1]"Transcription of a novel mouse semaphorin gene, M-semaH, correlates with the metastatic ability of mouse tumor cell lines."
Christensen C.R.L., Klingelhoefer J., Tarabykina S., Hulgaard E.F., Kramerov D., Lukanidin E.
Cancer Res. 58:1238-1244(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]Christensen C.R.L.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Developmental localization of semaphorin H messenger RNA acting as a collapsing factor on sensory axons in the mouse brain."
Miyazaki N., Furuyama T., Sakai T., Fujioka S., Mori T., Ohoka Y., Takeda N., Kubo T., Inagaki S.
Neuroscience 93:401-408(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57/Black 6.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[6]"Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins."
Gu C., Yoshida Y., Livet J., Reimert D.V., Mann F., Merte J., Henderson C.E., Jessell T.M., Kolodkin A.L., Ginty D.D.
Science 307:265-268(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, INTERACTION WITH PLXND1, TISSUE SPECIFICITY.
[7]"Specificity of sensory-motor connections encoded by Sema3e-Plxnd1 recognition."
Pecho-Vrieseling E., Sigrist M., Yoshida Y., Jessell T.M., Arber S.
Nature 459:842-846(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras."
Sakurai A., Gavard J., Annas-Linhares Y., Basile J.R., Amornphimoltham P., Palmby T.R., Yagi H., Zhang F., Randazzo P.A., Li X., Weigert R., Gutkind J.S.
Mol. Cell. Biol. 30:3086-3098(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PLXND1.
[9]"Semaphorin 3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum."
Ding J.B., Oh W.J., Sabatini B.L., Gu C.
Nat. Neurosci. 15:215-223(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z80941 mRNA. Translation: CAB02590.1.
Z93947 mRNA. Translation: CAB07987.1. Sequence problems.
Z93948 mRNA. Translation: CAB07988.1. Sequence problems.
AF034744 mRNA. Translation: AAD01996.1.
CH466635 Genomic DNA. Translation: EDL38710.1.
BC057956 mRNA. Translation: AAH57956.1.
RefSeqNP_035478.2. NM_011348.2.
UniGeneMm.134093.

3D structure databases

ProteinModelPortalP70275.
SMRP70275. Positions 31-522, 587-713.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP70275. 1 interaction.

PTM databases

PhosphoSiteP70275.

Proteomic databases

PRIDEP70275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000073957; ENSMUSP00000073612; ENSMUSG00000063531.
GeneID20349.
KEGGmmu:20349.
UCSCuc008wme.1. mouse.

Organism-specific databases

CTD9723.
MGIMGI:1340034. Sema3e.

Phylogenomic databases

eggNOGNOG324739.
GeneTreeENSGT00560000076660.
HOGENOMHOG000039964.
HOVERGENHBG055071.
InParanoidQ9QX23.
KOK06840.
OMAMIPDNED.
OrthoDBEOG7M0NQV.
TreeFamTF352628.

Gene expression databases

ArrayExpressP70275.
BgeeP70275.
GenevestigatorP70275.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015513. Semaphorin_3E.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERPTHR11036. PTHR11036. 1 hit.
PTHR11036:SF22. PTHR11036:SF22. 1 hit.
PfamPF00047. ig. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298199.
PROP70275.
SOURCESearch...

Entry information

Entry nameSEM3E_MOUSE
AccessionPrimary (citable) accession number: P70275
Secondary accession number(s): O09078, O09079, Q9QX23
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot