Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P70275

- SEM3E_MOUSE

UniProt

P70275 - SEM3E_MOUSE

Protein

Semaphorin-3E

Gene

Sema3e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. receptor activity Source: InterPro
    3. semaphorin receptor binding Source: UniProt

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. negative regulation of angiogenesis Source: UniProtKB
    3. negative regulation of cell-matrix adhesion Source: UniProtKB
    4. nervous system development Source: UniProtKB-KW
    5. patterning of blood vessels Source: UniProtKB
    6. regulation of actin cytoskeleton reorganization Source: UniProtKB
    7. regulation of cell shape Source: UniProtKB
    8. semaphorin-plexin signaling pathway Source: UniProtKB
    9. sprouting angiogenesis Source: UniProtKB
    10. synapse organization Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Semaphorin-3E
    Alternative name(s):
    Semaphorin-H
    Short name:
    Sema H
    Gene namesi
    Name:Sema3e
    Synonyms:Semah, Semh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1340034. Sema3e.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Embryos present defects in the patterning of intersomitic vasculature.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 775750Semaphorin-3EPRO_0000032318Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi105 ↔ 115By similarity
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi133 ↔ 142By similarity
    Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi270 ↔ 382By similarity
    Disulfide bondi294 ↔ 342By similarity
    Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi519 ↔ 537By similarity
    Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi654 ↔ 729By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP70275.

    PTM databases

    PhosphoSiteiP70275.

    Expressioni

    Tissue specificityi

    Detected in neurons in the thalamus. Detected in embryonic vasculature. Developing lungs, developing skeletal elements and ventral horns of the developing neural tube. Correlates positively with tumor progression.2 Publications

    Gene expression databases

    ArrayExpressiP70275.
    BgeeiP70275.
    GenevestigatoriP70275.

    Interactioni

    Subunit structurei

    Interacts with PLXND1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PLXND1Q9Y4D72EBI-8876322,EBI-310731From a different organism.

    Protein-protein interaction databases

    IntActiP70275. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP70275.
    SMRiP70275. Positions 31-522, 588-660.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 516485SemaPROSITE-ProRule annotationAdd
    BLAST
    Domaini581 – 66989Ig-like C2-typeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi737 – 77034Arg/Lys-rich (basic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the semaphorin family.Curated
    Contains 1 Sema domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Signal

    Phylogenomic databases

    eggNOGiNOG324739.
    GeneTreeiENSGT00560000076660.
    HOGENOMiHOG000039964.
    HOVERGENiHBG055071.
    InParanoidiQ9QX23.
    KOiK06840.
    OMAiEIHWPST.
    OrthoDBiEOG7M0NQV.
    TreeFamiTF352628.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR007110. Ig-like_dom.
    IPR003599. Ig_sub.
    IPR013151. Immunoglobulin.
    IPR016201. Plexin-like_fold.
    IPR001627. Semap_dom.
    IPR027231. Semaphorin.
    IPR015513. Semaphorin_3E.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PANTHERiPTHR11036. PTHR11036. 1 hit.
    PTHR11036:SF22. PTHR11036:SF22. 1 hit.
    PfamiPF00047. ig. 1 hit.
    PF01403. Sema. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    SM00423. PSI. 1 hit.
    SM00630. Sema. 1 hit.
    [Graphical view]
    SUPFAMiSSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    PROSITEiPS50835. IG_LIKE. 1 hit.
    PS51004. SEMA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70275-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPAGHILTL LLWGHLLELW TPGHSANPSY PRLRLSHKEL LELNRTSIFQ    50
    SPLGFLDLHT MLLDEYQERL FVGGRDLVYS LNLERVSDGY REIYWPSTAV 100
    KVEECIMKGK DANECANYIR VLHHYNRTHL LTCATGAFDP HCAFIRVGHH 150
    SEEPLFHLES HRSERGRGRC PFDPNSSFVS TLVGNELFAG LYSDYWGRDS 200
    AIFRSMGKLG HIRTEHDDER LLKEPKFVGS YMIPDNEDRD DNKMYFFFTE 250
    KALEAENNAH TIYTRVGRLC VNDMGGQRIL VNKWSTFLKA RLVCSVPGMN 300
    GIDTYFDELE DVFLLPTRDP KNPVIFGLFN TTSNIFRGHA VCVYHMSSIR 350
    EAFNGPYAHK EGPEYHWSLY EGKVPYPRPG SCASKVNGGK YGTTKDYPDD 400
    AIRFARMHPL MYQPIKPVHK KPILVKTDGK YNLRQLAVDR VEAEDGQYDV 450
    LFIGTDTGIV LKVITIYNQE TEWMEEVILE ELQIFKDPAP IISMEISSKR 500
    QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD GISCSRYYPT 550
    GAHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDRTEERLA YGIESNSTLL 600
    ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG 650
    TYFCQTVEHN FVHTVRKITL EVVEEHKVEG MFHKDHEEER HHKMPCPPLS 700
    GMSQGTKPWY KEFLQLIGYS NFQRVEEYCE KVWCTDKKRK KLKMSPSKWK 750
    YANPQEKRLR SKAEHFRLPR HTLLS 775
    Length:775
    Mass (Da):89,543
    Last modified:July 27, 2011 - v3
    Checksum:i221E766F404098D4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti407 – 4082MH → ID in CAB02590. (PubMed:9515811)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z80941 mRNA. Translation: CAB02590.1.
    Z93947 mRNA. Translation: CAB07987.1. Sequence problems.
    Z93948 mRNA. Translation: CAB07988.1. Sequence problems.
    AF034744 mRNA. Translation: AAD01996.1.
    CH466635 Genomic DNA. Translation: EDL38710.1.
    BC057956 mRNA. Translation: AAH57956.1.
    CCDSiCCDS19093.1.
    RefSeqiNP_035478.2. NM_011348.2.
    UniGeneiMm.134093.

    Genome annotation databases

    EnsembliENSMUST00000073957; ENSMUSP00000073612; ENSMUSG00000063531.
    GeneIDi20349.
    KEGGimmu:20349.
    UCSCiuc008wme.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z80941 mRNA. Translation: CAB02590.1 .
    Z93947 mRNA. Translation: CAB07987.1 . Sequence problems.
    Z93948 mRNA. Translation: CAB07988.1 . Sequence problems.
    AF034744 mRNA. Translation: AAD01996.1 .
    CH466635 Genomic DNA. Translation: EDL38710.1 .
    BC057956 mRNA. Translation: AAH57956.1 .
    CCDSi CCDS19093.1.
    RefSeqi NP_035478.2. NM_011348.2.
    UniGenei Mm.134093.

    3D structure databases

    ProteinModelPortali P70275.
    SMRi P70275. Positions 31-522, 588-660.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P70275. 1 interaction.

    PTM databases

    PhosphoSitei P70275.

    Proteomic databases

    PRIDEi P70275.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000073957 ; ENSMUSP00000073612 ; ENSMUSG00000063531 .
    GeneIDi 20349.
    KEGGi mmu:20349.
    UCSCi uc008wme.1. mouse.

    Organism-specific databases

    CTDi 9723.
    MGIi MGI:1340034. Sema3e.

    Phylogenomic databases

    eggNOGi NOG324739.
    GeneTreei ENSGT00560000076660.
    HOGENOMi HOG000039964.
    HOVERGENi HBG055071.
    InParanoidi Q9QX23.
    KOi K06840.
    OMAi EIHWPST.
    OrthoDBi EOG7M0NQV.
    TreeFami TF352628.

    Miscellaneous databases

    NextBioi 298199.
    PROi P70275.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70275.
    Bgeei P70275.
    Genevestigatori P70275.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR007110. Ig-like_dom.
    IPR003599. Ig_sub.
    IPR013151. Immunoglobulin.
    IPR016201. Plexin-like_fold.
    IPR001627. Semap_dom.
    IPR027231. Semaphorin.
    IPR015513. Semaphorin_3E.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    PANTHERi PTHR11036. PTHR11036. 1 hit.
    PTHR11036:SF22. PTHR11036:SF22. 1 hit.
    Pfami PF00047. ig. 1 hit.
    PF01403. Sema. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    SM00423. PSI. 1 hit.
    SM00630. Sema. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    PROSITEi PS50835. IG_LIKE. 1 hit.
    PS51004. SEMA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcription of a novel mouse semaphorin gene, M-semaH, correlates with the metastatic ability of mouse tumor cell lines."
      Christensen C.R.L., Klingelhoefer J., Tarabykina S., Hulgaard E.F., Kramerov D., Lukanidin E.
      Cancer Res. 58:1238-1244(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. Christensen C.R.L.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Developmental localization of semaphorin H messenger RNA acting as a collapsing factor on sensory axons in the mouse brain."
      Miyazaki N., Furuyama T., Sakai T., Fujioka S., Mori T., Ohoka Y., Takeda N., Kubo T., Inagaki S.
      Neuroscience 93:401-408(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57/Black 6.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    6. "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins."
      Gu C., Yoshida Y., Livet J., Reimert D.V., Mann F., Merte J., Henderson C.E., Jessell T.M., Kolodkin A.L., Ginty D.D.
      Science 307:265-268(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION, INTERACTION WITH PLXND1, TISSUE SPECIFICITY.
    7. "Specificity of sensory-motor connections encoded by Sema3e-Plxnd1 recognition."
      Pecho-Vrieseling E., Sigrist M., Yoshida Y., Jessell T.M., Arber S.
      Nature 459:842-846(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras."
      Sakurai A., Gavard J., Annas-Linhares Y., Basile J.R., Amornphimoltham P., Palmby T.R., Yagi H., Zhang F., Randazzo P.A., Li X., Weigert R., Gutkind J.S.
      Mol. Cell. Biol. 30:3086-3098(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PLXND1.
    9. "Semaphorin 3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum."
      Ding J.B., Oh W.J., Sabatini B.L., Gu C.
      Nat. Neurosci. 15:215-223(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSEM3E_MOUSE
    AccessioniPrimary (citable) accession number: P70275
    Secondary accession number(s): O09078, O09079, Q9QX23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3