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P70275

- SEM3E_MOUSE

UniProt

P70275 - SEM3E_MOUSE

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Protein

Semaphorin-3E

Gene

Sema3e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.4 Publications

GO - Molecular functioni

  1. receptor activity Source: InterPro
  2. semaphorin receptor binding Source: UniProt

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. negative regulation of angiogenesis Source: UniProtKB
  3. negative regulation of cell-matrix adhesion Source: UniProtKB
  4. nervous system development Source: UniProtKB-KW
  5. patterning of blood vessels Source: UniProtKB
  6. regulation of actin cytoskeleton reorganization Source: UniProtKB
  7. regulation of cell shape Source: UniProtKB
  8. semaphorin-plexin signaling pathway Source: UniProtKB
  9. sprouting angiogenesis Source: UniProtKB
  10. synapse organization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Angiogenesis, Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiREACT_231928. Other semaphorin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-3E
Alternative name(s):
Semaphorin-H
Short name:
Sema H
Gene namesi
Name:Sema3e
Synonyms:Semah, Semh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1340034. Sema3e.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Embryos present defects in the patterning of intersomitic vasculature.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 775750Semaphorin-3EPRO_0000032318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi105 ↔ 115By similarity
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi133 ↔ 142By similarity
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi270 ↔ 382By similarity
Disulfide bondi294 ↔ 342By similarity
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi519 ↔ 537By similarity
Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi654 ↔ 729By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70275.
PRIDEiP70275.

PTM databases

PhosphoSiteiP70275.

Expressioni

Tissue specificityi

Detected in neurons in the thalamus. Detected in embryonic vasculature. Developing lungs, developing skeletal elements and ventral horns of the developing neural tube. Correlates positively with tumor progression.2 Publications

Gene expression databases

BgeeiP70275.
ExpressionAtlasiP70275. baseline and differential.
GenevestigatoriP70275.

Interactioni

Subunit structurei

Interacts with PLXND1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PLXND1Q9Y4D72EBI-8876322,EBI-310731From a different organism.

Protein-protein interaction databases

IntActiP70275. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP70275.
SMRiP70275. Positions 31-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 516485SemaPROSITE-ProRule annotationAdd
BLAST
Domaini581 – 66989Ig-like C2-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi737 – 77034Arg/Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Belongs to the semaphorin family.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiNOG324739.
GeneTreeiENSGT00760000118854.
HOGENOMiHOG000039964.
HOVERGENiHBG055071.
InParanoidiP70275.
KOiK06840.
OMAiEIHWPST.
OrthoDBiEOG7M0NQV.
TreeFamiTF352628.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015513. Semaphorin_3E.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PTHR11036:SF22. PTHR11036:SF22. 1 hit.
PfamiPF00047. ig. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70275-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPAGHILTL LLWGHLLELW TPGHSANPSY PRLRLSHKEL LELNRTSIFQ
60 70 80 90 100
SPLGFLDLHT MLLDEYQERL FVGGRDLVYS LNLERVSDGY REIYWPSTAV
110 120 130 140 150
KVEECIMKGK DANECANYIR VLHHYNRTHL LTCATGAFDP HCAFIRVGHH
160 170 180 190 200
SEEPLFHLES HRSERGRGRC PFDPNSSFVS TLVGNELFAG LYSDYWGRDS
210 220 230 240 250
AIFRSMGKLG HIRTEHDDER LLKEPKFVGS YMIPDNEDRD DNKMYFFFTE
260 270 280 290 300
KALEAENNAH TIYTRVGRLC VNDMGGQRIL VNKWSTFLKA RLVCSVPGMN
310 320 330 340 350
GIDTYFDELE DVFLLPTRDP KNPVIFGLFN TTSNIFRGHA VCVYHMSSIR
360 370 380 390 400
EAFNGPYAHK EGPEYHWSLY EGKVPYPRPG SCASKVNGGK YGTTKDYPDD
410 420 430 440 450
AIRFARMHPL MYQPIKPVHK KPILVKTDGK YNLRQLAVDR VEAEDGQYDV
460 470 480 490 500
LFIGTDTGIV LKVITIYNQE TEWMEEVILE ELQIFKDPAP IISMEISSKR
510 520 530 540 550
QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD GISCSRYYPT
560 570 580 590 600
GAHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDRTEERLA YGIESNSTLL
610 620 630 640 650
ECTPRSLQAK VIWFVQKGRD VRKEEVKTDD RVVKMDLGLL FLRVRKSDAG
660 670 680 690 700
TYFCQTVEHN FVHTVRKITL EVVEEHKVEG MFHKDHEEER HHKMPCPPLS
710 720 730 740 750
GMSQGTKPWY KEFLQLIGYS NFQRVEEYCE KVWCTDKKRK KLKMSPSKWK
760 770
YANPQEKRLR SKAEHFRLPR HTLLS
Length:775
Mass (Da):89,543
Last modified:July 27, 2011 - v3
Checksum:i221E766F404098D4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4082MH → ID in CAB02590. (PubMed:9515811)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80941 mRNA. Translation: CAB02590.1.
Z93947 mRNA. Translation: CAB07987.1. Sequence problems.
Z93948 mRNA. Translation: CAB07988.1. Sequence problems.
AF034744 mRNA. Translation: AAD01996.1.
CH466635 Genomic DNA. Translation: EDL38710.1.
BC057956 mRNA. Translation: AAH57956.1.
CCDSiCCDS19093.1.
RefSeqiNP_035478.2. NM_011348.2.
UniGeneiMm.134093.

Genome annotation databases

EnsembliENSMUST00000073957; ENSMUSP00000073612; ENSMUSG00000063531.
GeneIDi20349.
KEGGimmu:20349.
UCSCiuc008wme.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80941 mRNA. Translation: CAB02590.1 .
Z93947 mRNA. Translation: CAB07987.1 . Sequence problems.
Z93948 mRNA. Translation: CAB07988.1 . Sequence problems.
AF034744 mRNA. Translation: AAD01996.1 .
CH466635 Genomic DNA. Translation: EDL38710.1 .
BC057956 mRNA. Translation: AAH57956.1 .
CCDSi CCDS19093.1.
RefSeqi NP_035478.2. NM_011348.2.
UniGenei Mm.134093.

3D structure databases

ProteinModelPortali P70275.
SMRi P70275. Positions 31-522.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P70275. 1 interaction.

PTM databases

PhosphoSitei P70275.

Proteomic databases

MaxQBi P70275.
PRIDEi P70275.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000073957 ; ENSMUSP00000073612 ; ENSMUSG00000063531 .
GeneIDi 20349.
KEGGi mmu:20349.
UCSCi uc008wme.1. mouse.

Organism-specific databases

CTDi 9723.
MGIi MGI:1340034. Sema3e.

Phylogenomic databases

eggNOGi NOG324739.
GeneTreei ENSGT00760000118854.
HOGENOMi HOG000039964.
HOVERGENi HBG055071.
InParanoidi P70275.
KOi K06840.
OMAi EIHWPST.
OrthoDBi EOG7M0NQV.
TreeFami TF352628.

Enzyme and pathway databases

Reactomei REACT_231928. Other semaphorin interactions.

Miscellaneous databases

NextBioi 298199.
PROi P70275.
SOURCEi Search...

Gene expression databases

Bgeei P70275.
ExpressionAtlasi P70275. baseline and differential.
Genevestigatori P70275.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015513. Semaphorin_3E.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
PANTHERi PTHR11036. PTHR11036. 1 hit.
PTHR11036:SF22. PTHR11036:SF22. 1 hit.
Pfami PF00047. ig. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view ]
SUPFAMi SSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEi PS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Transcription of a novel mouse semaphorin gene, M-semaH, correlates with the metastatic ability of mouse tumor cell lines."
    Christensen C.R.L., Klingelhoefer J., Tarabykina S., Hulgaard E.F., Kramerov D., Lukanidin E.
    Cancer Res. 58:1238-1244(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. Christensen C.R.L.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Developmental localization of semaphorin H messenger RNA acting as a collapsing factor on sensory axons in the mouse brain."
    Miyazaki N., Furuyama T., Sakai T., Fujioka S., Mori T., Ohoka Y., Takeda N., Kubo T., Inagaki S.
    Neuroscience 93:401-408(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57/Black 6.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  6. "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins."
    Gu C., Yoshida Y., Livet J., Reimert D.V., Mann F., Merte J., Henderson C.E., Jessell T.M., Kolodkin A.L., Ginty D.D.
    Science 307:265-268(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, INTERACTION WITH PLXND1, TISSUE SPECIFICITY.
  7. "Specificity of sensory-motor connections encoded by Sema3e-Plxnd1 recognition."
    Pecho-Vrieseling E., Sigrist M., Yoshida Y., Jessell T.M., Arber S.
    Nature 459:842-846(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras."
    Sakurai A., Gavard J., Annas-Linhares Y., Basile J.R., Amornphimoltham P., Palmby T.R., Yagi H., Zhang F., Randazzo P.A., Li X., Weigert R., Gutkind J.S.
    Mol. Cell. Biol. 30:3086-3098(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PLXND1.
  9. "Semaphorin 3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum."
    Ding J.B., Oh W.J., Sabatini B.L., Gu C.
    Nat. Neurosci. 15:215-223(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSEM3E_MOUSE
AccessioniPrimary (citable) accession number: P70275
Secondary accession number(s): O09078, O09079, Q9QX23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3