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Protein

Serine/threonine-protein kinase N1

Gene

Pkn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Enzyme regulationi

Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-778 (activation loop of the kinase domain) and Ser-920 (turn motif), need to be phosphorylated for its full activation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei648 – 6481ATPPROSITE-ProRule annotation
Active sitei744 – 7441Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi625 – 6339ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • B cell apoptotic process Source: MGI
  • B cell homeostasis Source: MGI
  • epithelial cell migration Source: UniProtKB
  • histone H3-T11 phosphorylation Source: UniProtKB
  • hyperosmotic response Source: MGI
  • intracellular signal transduction Source: GO_Central
  • negative regulation of B cell proliferation Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: MGI
  • regulation of cell motility Source: UniProtKB
  • regulation of germinal center formation Source: MGI
  • regulation of immunoglobulin production Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • renal system process Source: MGI
  • spleen development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5625886. Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase N1 (EC:2.7.11.13By similarity)
Alternative name(s):
Protein kinase C-like 1
Protein kinase C-like PKN
Protein-kinase C-related kinase 1
Serine-threonine protein kinase N
Gene namesi
Name:Pkn1
Synonyms:Pkn, Prk1, Prkcl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:108022. Pkn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Endosome By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Cleavage furrow By similarity
  • Midbody By similarity

  • Note: Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.By similarity

GO - Cellular componenti

  • cleavage furrow Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • endosome Source: UniProtKB
  • midbody Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 946945Serine/threonine-protein kinase N1PRO_0000055720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei451 – 4511N6-acetyllysineBy similarity
Modified residuei536 – 5361PhosphoserineCombined sources
Modified residuei540 – 5401PhosphoserineCombined sources
Modified residuei562 – 5621PhosphoserineBy similarity
Modified residuei565 – 5651PhosphoserineBy similarity
Modified residuei612 – 6121PhosphoserineBy similarity
Modified residuei778 – 7781Phosphothreonine; by PDPK1By similarity
Modified residuei782 – 7821PhosphothreonineBy similarity
Modified residuei918 – 9181PhosphothreonineCombined sources
Modified residuei920 – 9201PhosphoserineCombined sources

Post-translational modificationi

Autophosphorylated; preferably on serine.By similarity
Activated by limited proteolysis with trypsin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei108 – 1092Cleavage; by caspase-3By similarity
Sitei457 – 4582Cleavage; by caspase-3By similarity
Sitei561 – 5622Cleavage; by caspase-3By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70268.
MaxQBiP70268.
PaxDbiP70268.
PeptideAtlasiP70268.
PRIDEiP70268.

PTM databases

iPTMnetiP70268.
PhosphoSiteiP70268.

Miscellaneous databases

PMAP-CutDBP70268.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057672.
CleanExiMM_PKN1.
ExpressionAtlasiP70268. baseline and differential.
GenevisibleiP70268. MM.

Interactioni

Subunit structurei

Interacts with ZFAND6 (PubMed:11054541). Interacts with ANDR. Interacts with PRKCB. Interacts (via REM 1 and REM 2 repeats) with RAC1 (By similarity). Interacts (via REM 1 repeat) with RHOA (PubMed:8571127). Interacts with RHOB. Interacts (via C-terminus) with PDPK1. Interacts with CCNT2; enhances MYOD1-dependent transcription. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000116235.

Structurei

3D structure databases

ProteinModelPortaliP70268.
SMRiP70268. Positions 13-98, 125-190, 539-944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati34 – 11077REM 1Add
BLAST
Repeati123 – 21290REM 2Add
BLAST
Repeati213 – 29482REM 3Add
BLAST
Domaini328 – 464137C2Add
BLAST
Domaini619 – 878260Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini879 – 94668AGC-kinase C-terminalAdd
BLAST

Domaini

The C1 domain does not bind the diacylglycerol (DAG).By similarity

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 3 REM (Hr1) repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233032.
HOVERGENiHBG108317.
InParanoidiP70268.
KOiK06071.
OMAiEFRSSGE.
OrthoDBiEOG091G00YT.
PhylomeDBiP70268.
TreeFamiTF102005.

Family and domain databases

Gene3Di1.10.287.160. 3 hits.
2.60.40.150. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P70268-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL
60 70 80 90 100
KLKEGAENLR RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL
110 120 130 140 150
PDPAAGSDAT QSLAEGSPIC SSTNLSRVAG LEKQLAIELK VKQGAENMIQ
160 170 180 190 200
TYSNGSSKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA LQAGELESQA
210 220 230 240 250
APDEAQGDPE LGAVELRIEE LRHHFRVEHA VAEGAKNVLR LLSGAKAPDR
260 270 280 290 300
KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS
310 320 330 340 350
SAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS
360 370 380 390 400
VGASGTPESR TPFLSRPARG LYSRSGSLSG RSSLRGEAEN ATEVSTVLKL
410 420 430 440 450
DNTVVGQTAW KPCGPNAWDQ SFTLELERAR ELELAVFWRD QRGLCALKFL
460 470 480 490 500
KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR NPIIERIPRL QRQKKIFSKQ
510 520 530 540 550
QGKAFQRARQ MNIDVATWVR LLRRLIPSAV ATGTFSPNAS PGAEIRHTGD
560 570 580 590 600
ISMEKLNLGA DSDSSSQKSP PGLPSTSCSL SSPTHESTTS PELPSETQET
610 620 630 640 650
PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFRS SGELFAIKAL
660 670 680 690 700
KKGDIVARDE VESLMCEKRI LAAVTRAGHP FLVNLFGCFQ TPEHVCFVME
710 720 730 740 750
YSAGGDLMLH IHSDVFSEPR AVFYSACVVL GLQFLHEHKI VYRDLKLDNL
760 770 780 790 800
LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC GTPEFLAPEV LTDTSYTRAV
810 820 830 840 850
DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR FLSAEAIGIM
860 870 880 890 900
RRLLRRNPER RLGSTERDAE DVKKQPFFRS LGWDVLLARR LPPPFVPTLS
910 920 930 940
GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY
Length:946
Mass (Da):104,411
Last modified:November 23, 2004 - v3
Checksum:iFCB4E61AD27C1271
GO
Isoform 2 (identifier: P70268-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MAGDAVQ → MAADPPLDSELE

Show »
Length:951
Mass (Da):105,007
Checksum:i536F65AD12BA476C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 77MAGDAVQ → MAADPPLDSELE in isoform 2. CuratedVSP_039223

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089431 mRNA. Translation: BAC40880.2.
AK149649 mRNA. Translation: BAE29005.1.
BC052923 mRNA. Translation: AAH52923.1.
Y07611 Genomic DNA. Translation: CAA68883.1.
CCDSiCCDS22459.1. [P70268-1]
CCDS57627.1. [P70268-2]
PIRiPC4220.
RefSeqiNP_001186522.1. NM_001199593.1. [P70268-2]
NP_796236.2. NM_177262.4. [P70268-1]
UniGeneiMm.213000.

Genome annotation databases

EnsembliENSMUST00000005616; ENSMUSP00000005616; ENSMUSG00000057672. [P70268-1]
ENSMUST00000144258; ENSMUSP00000116235; ENSMUSG00000057672. [P70268-2]
GeneIDi320795.
KEGGimmu:320795.
UCSCiuc009mkw.2. mouse. [P70268-2]
uc009mkx.2. mouse. [P70268-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089431 mRNA. Translation: BAC40880.2.
AK149649 mRNA. Translation: BAE29005.1.
BC052923 mRNA. Translation: AAH52923.1.
Y07611 Genomic DNA. Translation: CAA68883.1.
CCDSiCCDS22459.1. [P70268-1]
CCDS57627.1. [P70268-2]
PIRiPC4220.
RefSeqiNP_001186522.1. NM_001199593.1. [P70268-2]
NP_796236.2. NM_177262.4. [P70268-1]
UniGeneiMm.213000.

3D structure databases

ProteinModelPortaliP70268.
SMRiP70268. Positions 13-98, 125-190, 539-944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000116235.

PTM databases

iPTMnetiP70268.
PhosphoSiteiP70268.

Proteomic databases

EPDiP70268.
MaxQBiP70268.
PaxDbiP70268.
PeptideAtlasiP70268.
PRIDEiP70268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005616; ENSMUSP00000005616; ENSMUSG00000057672. [P70268-1]
ENSMUST00000144258; ENSMUSP00000116235; ENSMUSG00000057672. [P70268-2]
GeneIDi320795.
KEGGimmu:320795.
UCSCiuc009mkw.2. mouse. [P70268-2]
uc009mkx.2. mouse. [P70268-1]

Organism-specific databases

CTDi5585.
MGIiMGI:108022. Pkn1.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233032.
HOVERGENiHBG108317.
InParanoidiP70268.
KOiK06071.
OMAiEFRSSGE.
OrthoDBiEOG091G00YT.
PhylomeDBiP70268.
TreeFamiTF102005.

Enzyme and pathway databases

ReactomeiR-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5625886. Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.

Miscellaneous databases

ChiTaRSiPkn1. mouse.
PMAP-CutDBP70268.
PROiP70268.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057672.
CleanExiMM_PKN1.
ExpressionAtlasiP70268. baseline and differential.
GenevisibleiP70268. MM.

Family and domain databases

Gene3Di1.10.287.160. 3 hits.
2.60.40.150. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR011072. HR1_rho-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02185. HR1. 3 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00742. Hr1. 3 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 3 hits.
SSF49562. SSF49562. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKN1_MOUSE
AccessioniPrimary (citable) accession number: P70268
Secondary accession number(s): Q3UEA6, Q7TST2, Q8BTL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 160 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.