Reviewed, UniProtKB/Swiss-Prot P70265 (F262_MOUSE)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Alternative name(s):
    PFK-2/FBPase-2
    6PF-2-K/Fru-2,6-P2ASE heart-type isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

Pfkfb2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme By similarity.
Subunit structure	Homodimer By similarity.
Tissue specificity	Highest levels in kidney; also found in heart, brain, spleen, lung, liver, skeletal muscle and testis.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		PRO_0000179965
Regions
	Nucleotide binding	48 – 55	8	ATP Potential
	Region	1 – 251	251	6-phosphofructo-2-kinase
	Region	252 – 519	268	Fructose-2,6-bisphosphatase
Sites
	Active site	131	1	Potential
	Active site	161	1	Potential
	Active site	260	1	Tele-phosphohistidine intermediate By similarity
	Active site	329	1	Potential
	Active site	394	1	Proton donor By similarity
	Binding site	105	1	Fructose-6-phosphate By similarity
	Binding site	196	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	32	1	Phosphoserine; by PKA By similarity
	Modified residue	469	1	Phosphoserine; by PKA By similarity
	Modified residue	471	1	Phosphothreonine By similarity
	Modified residue	478	1	Phosphothreonine; by PKC By similarity
	Modified residue	486	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	9	1	T → P in AAH18418. Ref.2
	Sequence conflict	12	1	Missing in AAH18418. Ref.2
	Sequence conflict	222	1	M → I in AAH18418. Ref.2
	Sequence conflict	496	1	K → S in AAH18418. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P70265-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: ACD4F41AEDF22F9A	FASTA	519	59,967
	10 20 30 40 50 60 MSENSTFSTE DSCNSSYKPH ASNLRRAGKT CSWASYMTNS PTLIVMIGLP ARGKTYVSKK 70 80 90 100 110 120 LTRYLNWIGV PTKVFNLGVY RREAVKSYQS YDFFRHDNEE AMKIRKQCAL VALEDVKAYF 130 140 150 160 170 180 TEESGQIAVF DATNTTRERR DMILNFAKQN AFKVFFVESV CDDPDVIAAN ILEVKVSSPD 190 200 210 220 230 240 YPERNRENVM EDFLKRIECY KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK 250 260 270 280 290 300 IVYYLMNIHV HPRTIYLCRH GESEFNLLGK IGGDSGLSVR GKQFAHALKK FLEEQEIQDL 310 320 330 340 350 360 KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE FALRDQEKYL 370 380 390 400 410 420 YRYPGGESYQ DLVQRLEPVI MELERQGNIL VISHQAVMRC LLAYFLDKGA DELPYLRCPL 430 440 450 460 470 480 HIIFKLTPVA YGCKVETITL NVDAVDTHRD KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR 490 500 510 RPRNYSVGSR PLKPLKPLRA LDMQEGADQP KTQVSIPVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and tissue-specific expression of mouse kidney 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase." Batra R.S., Brown R.M., Brown G.K., Craig I.W. FEBS Lett. 393:167-173(1996) [PubMed: 8814283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98847 mRNA. Translation: CAA67352.1. BC018418 mRNA. Translation: AAH18418.1.
IPI	IPI00137533.
PIR	S74242.
UniGene	Mm.249861
3D structure databases
HSSP	HSSP built from PDB template 1C80 based on UniProtKB P07953.
ModBase	Search...
PTM databases
PhosphoSite	P70265.
Genome annotation databases
Ensembl	ENSMUSG00000026409. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107815. Pfkfb2.
Phylogenomic databases
HOGENOM	P70265.
HOVERGEN	P70265.
Enzyme and pathway databases
BRENDA	2.7.1.105. 244. 3.1.3.46. 244.
Gene expression databases
ArrayExpress	P70265.
Bgee	P70265.
GermOnline	ENSMUSG00000026409. Mus musculus.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

F262_MOUSE

Accession

Primary (citable) accession number: P70265
Secondary accession number(s): Q8VEI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)