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P70265

- F262_MOUSE

UniProt

P70265 - F262_MOUSE

Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Gene

Pfkfb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Synthesis and degradation of fructose 2,6-bisphosphate.

    Catalytic activityi

    Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
    ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

    Enzyme regulationi

    The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811Fructose 6-phosphateBy similarity
    Binding sitei105 – 1051Fructose 6-phosphateBy similarity
    Active sitei131 – 1311Sequence Analysis
    Binding sitei133 – 1331Fructose 6-phosphateBy similarity
    Binding sitei139 – 1391Fructose 6-phosphateBy similarity
    Active sitei161 – 1611Sequence Analysis
    Binding sitei175 – 1751Fructose 6-phosphateBy similarity
    Binding sitei196 – 1961Fructose 6-phosphateBy similarity
    Binding sitei200 – 2001Fructose 6-phosphateBy similarity
    Binding sitei259 – 2591Fructose 2,6-bisphosphateBy similarity
    Active sitei260 – 2601Tele-phosphohistidine intermediateBy similarity
    Binding sitei266 – 2661Fructose 2,6-bisphosphateBy similarity
    Binding sitei272 – 2721Fructose 2,6-bisphosphate; via amide nitrogenBy similarity
    Binding sitei309 – 3091Fructose 2,6-bisphosphateBy similarity
    Active sitei329 – 3291Proton donor/acceptorBy similarity
    Binding sitei340 – 3401Fructose 2,6-bisphosphateBy similarity
    Binding sitei354 – 3541Fructose 2,6-bisphosphateBy similarity
    Binding sitei358 – 3581Fructose 2,6-bisphosphateBy similarity
    Binding sitei369 – 3691Fructose 2,6-bisphosphateBy similarity
    Active sitei394 – 3941Proton donor/acceptorBy similarity
    Binding sitei395 – 3951Fructose 2,6-bisphosphateBy similarity
    Binding sitei399 – 3991Fructose 2,6-bisphosphateBy similarity
    Binding sitei431 – 4311ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi48 – 569ATPBy similarity
    Nucleotide bindingi170 – 1756ATPBy similarity
    Nucleotide bindingi351 – 3544ATPBy similarity
    Nucleotide bindingi395 – 3995ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructo-2-kinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. fructose-2,6-bisphosphate 2-phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 2,6-bisphosphate metabolic process Source: InterPro
    3. fructose metabolic process Source: InterPro
    4. glycolytic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.1.3.46. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
    Short name:
    6PF-2-K/Fru-2,6-P2ase 2
    Short name:
    PFK/FBPase 2
    Alternative name(s):
    6PF-2-K/Fru-2,6-P2ase heart-type isozyme
    Including the following 2 domains:
    Fructose-2,6-bisphosphatase (EC:3.1.3.46)
    Gene namesi
    Name:Pfkfb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:107815. Pfkfb2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 5195186-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2PRO_0000179965Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei32 – 321Phosphoserine; by PKABy similarity
    Modified residuei469 – 4691Phosphoserine; by AMPK and PKA
    Modified residuei471 – 4711PhosphothreonineBy similarity
    Modified residuei478 – 4781Phosphothreonine; by PKCBy similarity
    Modified residuei486 – 4861PhosphoserineBy similarity
    Modified residuei496 – 4961PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by AMPK stimulates activity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP70265.
    PaxDbiP70265.
    PRIDEiP70265.

    PTM databases

    PhosphoSiteiP70265.

    Expressioni

    Tissue specificityi

    Highest levels in kidney; also found in heart, brain, spleen, lung, liver, skeletal muscle and testis.

    Gene expression databases

    GenevestigatoriP70265.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi202123. 1 interaction.
    IntActiP70265. 2 interactions.
    STRINGi10090.ENSMUSP00000066426.

    Structurei

    3D structure databases

    ProteinModelPortaliP70265.
    SMRiP70265. Positions 40-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 2512506-phosphofructo-2-kinaseAdd
    BLAST
    Regioni252 – 519268Fructose-2,6-bisphosphataseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

    Phylogenomic databases

    eggNOGiCOG0406.
    HOGENOMiHOG000181112.
    HOVERGENiHBG005628.
    InParanoidiP70265.
    KOiK01103.
    PhylomeDBiP70265.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003094. 6Pfruct_kin.
    IPR013079. 6Phosfructo_kin.
    IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
    IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR027417. P-loop_NTPase.
    IPR001345. PG/BPGM_mutase_AS.
    [Graphical view]
    PANTHERiPTHR10606. PTHR10606. 1 hit.
    PfamiPF01591. 6PF2K. 1 hit.
    PF00300. His_Phos_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000709. 6PFK_2-Ptase. 1 hit.
    PRINTSiPR00991. 6PFRUCTKNASE.
    SMARTiSM00855. PGAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF53254. SSF53254. 1 hit.
    PROSITEiPS00175. PG_MUTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70265-1 [UniParc]FASTAAdd to Basket

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    MSENSTFSTE DSCNSSYKPH ASNLRRAGKT CSWASYMTNS PTLIVMIGLP    50
    ARGKTYVSKK LTRYLNWIGV PTKVFNLGVY RREAVKSYQS YDFFRHDNEE 100
    AMKIRKQCAL VALEDVKAYF TEESGQIAVF DATNTTRERR DMILNFAKQN 150
    AFKVFFVESV CDDPDVIAAN ILEVKVSSPD YPERNRENVM EDFLKRIECY 200
    KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK IVYYLMNIHV 250
    HPRTIYLCRH GESEFNLLGK IGGDSGLSVR GKQFAHALKK FLEEQEIQDL 300
    KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE 350
    FALRDQEKYL YRYPGGESYQ DLVQRLEPVI MELERQGNIL VISHQAVMRC 400
    LLAYFLDKGA DELPYLRCPL HIIFKLTPVA YGCKVETITL NVDAVDTHRD 450
    KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR RPRNYSVGSR PLKPLSPLRA 500
    LDMQEGADQP KTQVSIPVV 519
    Length:519
    Mass (Da):59,925
    Last modified:September 1, 2009 - v2
    Checksum:iFD4CF41AEDF22F89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91T → P in AAH18418. (PubMed:15489334)Curated
    Sequence conflicti12 – 121Missing in AAH18418. (PubMed:15489334)Curated
    Sequence conflicti222 – 2221M → I in AAH18418. (PubMed:15489334)Curated
    Sequence conflicti496 – 4961S → K in CAA67352. (PubMed:8814283)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98847 mRNA. Translation: CAA67352.1.
    BC018418 mRNA. Translation: AAH18418.1.
    PIRiS74242.
    RefSeqiNP_032851.2. NM_008825.4.
    UniGeneiMm.249861.

    Genome annotation databases

    GeneIDi18640.
    KEGGimmu:18640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98847 mRNA. Translation: CAA67352.1 .
    BC018418 mRNA. Translation: AAH18418.1 .
    PIRi S74242.
    RefSeqi NP_032851.2. NM_008825.4.
    UniGenei Mm.249861.

    3D structure databases

    ProteinModelPortali P70265.
    SMRi P70265. Positions 40-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202123. 1 interaction.
    IntActi P70265. 2 interactions.
    STRINGi 10090.ENSMUSP00000066426.

    PTM databases

    PhosphoSitei P70265.

    Proteomic databases

    MaxQBi P70265.
    PaxDbi P70265.
    PRIDEi P70265.

    Protocols and materials databases

    DNASUi 18640.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18640.
    KEGGi mmu:18640.

    Organism-specific databases

    CTDi 5208.
    MGIi MGI:107815. Pfkfb2.

    Phylogenomic databases

    eggNOGi COG0406.
    HOGENOMi HOG000181112.
    HOVERGENi HBG005628.
    InParanoidi P70265.
    KOi K01103.
    PhylomeDBi P70265.

    Enzyme and pathway databases

    BRENDAi 3.1.3.46. 3474.

    Miscellaneous databases

    NextBioi 294622.
    PROi P70265.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P70265.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003094. 6Pfruct_kin.
    IPR013079. 6Phosfructo_kin.
    IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
    IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR027417. P-loop_NTPase.
    IPR001345. PG/BPGM_mutase_AS.
    [Graphical view ]
    PANTHERi PTHR10606. PTHR10606. 1 hit.
    Pfami PF01591. 6PF2K. 1 hit.
    PF00300. His_Phos_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000709. 6PFK_2-Ptase. 1 hit.
    PRINTSi PR00991. 6PFRUCTKNASE.
    SMARTi SM00855. PGAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF53254. SSF53254. 1 hit.
    PROSITEi PS00175. PG_MUTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and tissue-specific expression of mouse kidney 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase."
      Batra R.S., Brown R.M., Brown G.K., Craig I.W.
      FEBS Lett. 393:167-173(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiF262_MOUSE
    AccessioniPrimary (citable) accession number: P70265
    Secondary accession number(s): Q8VEI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3