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P70259

- GP143_MOUSE

UniProt

P70259 - GP143_MOUSE

Protein

G-protein coupled receptor 143

Gene

Gpr143

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca2+ influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.1 Publication

    GO - Molecular functioni

    1. dopamine binding Source: UniProtKB
    2. G-protein coupled receptor activity Source: UniProtKB
    3. L-DOPA binding Source: UniProtKB
    4. L-DOPA receptor activity Source: UniProtKB
    5. tyrosine binding Source: UniProtKB

    GO - Biological processi

    1. calcium-mediated signaling using intracellular calcium source Source: UniProtKB
    2. G-protein coupled receptor signaling pathway Source: UniProtKB
    3. melanosome localization Source: UniProtKB
    4. melanosome organization Source: UniProtKB
    5. melanosome transport Source: UniProtKB
    6. neuropeptide signaling pathway Source: GOC
    7. phosphatidylinositol-mediated signaling Source: UniProtKB
    8. regulation of calcium-mediated signaling Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-protein coupled receptor 143
    Alternative name(s):
    MOA1
    Ocular albinism type 1 protein homolog
    Gene namesi
    Name:Gpr143
    Synonyms:Oa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:107193. Gpr143.

    Subcellular locationi

    Golgi apparatus By similarity. Melanosome membrane By similarity; Multi-pass membrane protein By similarity. Lysosome membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. Golgi apparatus Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW
    4. lysosomal membrane Source: UniProtKB
    5. melanosome Source: UniProtKB
    6. melanosome membrane Source: UniProtKB
    7. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Golgi apparatus, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 405405G-protein coupled receptor 143PRO_0000195087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.By similarity
    Phosphorylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP70259.

    PTM databases

    PhosphoSiteiP70259.

    Expressioni

    Gene expression databases

    BgeeiP70259.
    CleanExiMM_GPR143.
    GenevestigatoriP70259.

    Interactioni

    Subunit structurei

    Interacts with heterotrimeric G(i) proteins. Interacts with ARRB1 and ARRB2. Interacts with MLANA By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026383.

    Structurei

    3D structure databases

    ProteinModelPortaliP70259.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2727ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini49 – 7830CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini100 – 12425ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini146 – 1538CytoplasmicSequence Analysis
    Topological domaini175 – 19117ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini213 – 24836CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini270 – 29223ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini314 – 40592CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 4821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei79 – 9921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei125 – 14521Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei249 – 26921Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei293 – 31321Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni221 – 23818Necessary for its G protein-activation ability and normal distribution of melanosomesBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi222 – 23110lysosomal/melanosomal membrane localization signalBy similarity
    Motifi329 – 3302lysosomal/melanosomal membrane localization signalBy similarity

    Domaini

    The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39873.
    HOGENOMiHOG000112769.
    HOVERGENiHBG051784.
    InParanoidiP70259.
    KOiK08470.
    OMAiMGILNPM.
    OrthoDBiEOG7HXCQZ.
    PhylomeDBiP70259.
    TreeFamiTF324849.

    Family and domain databases

    InterProiIPR001414. Ocular_alb1.
    [Graphical view]
    PfamiPF02101. Ocular_alb. 1 hit.
    [Graphical view]
    PRINTSiPR00965. OCULARALBNSM.

    Sequencei

    Sequence statusi: Complete.

    P70259-1 [UniParc]FASTAAdd to Basket

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    MASPRLGIFC CPTWDAATQL VLSFQPRVFH ALCLGSGTLR LVLGLLQLLS    50
    GRRSVGHRAP ATSPAASVHI LRAATACDLL GCLGIVIRST VWIAYPEFIE 100
    NISNVNATDI WPATFCVGSA MWIQLLYSAC FWWLFCYAVD VYLVIRRSAG 150
    RSTILLYHIM AWGLAVLLCV EGAVMLYYPS VSRCERGLDH AIPHYVTTYL 200
    PLLLVLVANP ILFHKTVTSV ASLLKGRKGV YTENERLMGA VIKTRFFKIM 250
    LVLIACWLSN IINESLLFYL EMQPDIHGGS LKRIQNAART TWFIMGILNP 300
    AQGLLLSLAF YGWTGCSLDV HPPKMVIQWE TMTASAAEGT YQTPVRSCVP 350
    HQNPRKVVCV GGHTSDEVLS ILSEDSDAST VEIHTATGSC NIKEVDSISQ 400
    AQGEL 405
    Length:405
    Mass (Da):44,564
    Last modified:February 1, 1997 - v1
    Checksum:i716405A6512ED312
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti175 – 1751M → S in AAC52890. (PubMed:8921399)Curated
    Sequence conflicti213 – 2131F → L in AAC52890. (PubMed:8921399)Curated
    Sequence conflicti285 – 2851Q → L in AAC52890. (PubMed:8921399)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98352 Genomic DNA. Translation: CAA66996.1.
    U63918 mRNA. Translation: AAC52890.1.
    CCDSiCCDS30476.1.
    RefSeqiNP_035081.3. NM_010951.3.
    UniGeneiMm.5157.

    Genome annotation databases

    EnsembliENSMUST00000026383; ENSMUSP00000026383; ENSMUSG00000025333.
    GeneIDi18241.
    KEGGimmu:18241.
    UCSCiuc009uqo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98352 Genomic DNA. Translation: CAA66996.1 .
    U63918 mRNA. Translation: AAC52890.1 .
    CCDSi CCDS30476.1.
    RefSeqi NP_035081.3. NM_010951.3.
    UniGenei Mm.5157.

    3D structure databases

    ProteinModelPortali P70259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026383.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P70259.

    Proteomic databases

    PRIDEi P70259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026383 ; ENSMUSP00000026383 ; ENSMUSG00000025333 .
    GeneIDi 18241.
    KEGGi mmu:18241.
    UCSCi uc009uqo.1. mouse.

    Organism-specific databases

    CTDi 4935.
    MGIi MGI:107193. Gpr143.

    Phylogenomic databases

    eggNOGi NOG39873.
    HOGENOMi HOG000112769.
    HOVERGENi HBG051784.
    InParanoidi P70259.
    KOi K08470.
    OMAi MGILNPM.
    OrthoDBi EOG7HXCQZ.
    PhylomeDBi P70259.
    TreeFami TF324849.

    Miscellaneous databases

    NextBioi 293676.
    PROi P70259.
    SOURCEi Search...

    Gene expression databases

    Bgeei P70259.
    CleanExi MM_GPR143.
    Genevestigatori P70259.

    Family and domain databases

    InterProi IPR001414. Ocular_alb1.
    [Graphical view ]
    Pfami PF02101. Ocular_alb. 1 hit.
    [Graphical view ]
    PRINTSi PR00965. OCULARALBNSM.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the murine homolog of the ocular albinism type 1 (OA1) gene: sequence, genomic structure, and expression analysis in pigment cells."
      Bassi M.T., Incerti B., Easty D.J., Sviderskaya E.V., Ballabio A.
      Genome Res. 6:880-885(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Retina.
    2. "Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene."
      Newton J.M., Orlow S.J., Barsh G.S.
      Genomics 37:219-225(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells."
      Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., Schiaffino M.V.
      Hum. Mol. Genet. 17:3487-3501(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGP143_MOUSE
    AccessioniPrimary (citable) accession number: P70259
    Secondary accession number(s): O89031
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3