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P70259

- GP143_MOUSE

UniProt

P70259 - GP143_MOUSE

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Protein

G-protein coupled receptor 143

Gene

Gpr143

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca2+ influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.1 Publication

GO - Molecular functioni

  1. dopamine binding Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB
  3. L-DOPA binding Source: UniProtKB
  4. L-DOPA receptor activity Source: UniProtKB
  5. tyrosine binding Source: UniProtKB

GO - Biological processi

  1. calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  2. G-protein coupled receptor signaling pathway Source: UniProtKB
  3. melanosome localization Source: UniProtKB
  4. melanosome organization Source: UniProtKB
  5. melanosome transport Source: UniProtKB
  6. phosphatidylinositol-mediated signaling Source: UniProtKB
  7. regulation of calcium-mediated signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 143
Alternative name(s):
MOA1
Ocular albinism type 1 protein homolog
Gene namesi
Name:Gpr143
Synonyms:Oa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:107193. Gpr143.

Subcellular locationi

Golgi apparatus By similarity. Melanosome membrane By similarity; Multi-pass membrane protein By similarity. Lysosome membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727ExtracellularSequence AnalysisAdd
BLAST
Transmembranei28 – 4821Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini49 – 7830CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei79 – 9921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini100 – 12425ExtracellularSequence AnalysisAdd
BLAST
Transmembranei125 – 14521Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini146 – 1538CytoplasmicSequence Analysis
Transmembranei154 – 17421Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini175 – 19117ExtracellularSequence AnalysisAdd
BLAST
Transmembranei192 – 21221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini213 – 24836CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei249 – 26921Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini270 – 29223ExtracellularSequence AnalysisAdd
BLAST
Transmembranei293 – 31321Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini314 – 40592CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. Golgi apparatus Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. lysosomal membrane Source: UniProtKB
  5. melanosome Source: UniProtKB
  6. melanosome membrane Source: UniProtKB
  7. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405G-protein coupled receptor 143PRO_0000195087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.By similarity
Phosphorylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP70259.

PTM databases

PhosphoSiteiP70259.

Expressioni

Gene expression databases

BgeeiP70259.
CleanExiMM_GPR143.
ExpressionAtlasiP70259. differential.
GenevestigatoriP70259.

Interactioni

Subunit structurei

Interacts with heterotrimeric G(i) proteins. Interacts with ARRB1 and ARRB2. Interacts with MLANA (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026383.

Structurei

3D structure databases

ProteinModelPortaliP70259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 23818Necessary for its G protein-activation ability and normal distribution of melanosomesBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi222 – 23110lysosomal/melanosomal membrane localization signalBy similarity
Motifi329 – 3302lysosomal/melanosomal membrane localization signalBy similarity

Domaini

The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39873.
HOGENOMiHOG000112769.
HOVERGENiHBG051784.
InParanoidiP70259.
KOiK08470.
OMAiMGILNPM.
OrthoDBiEOG7HXCQZ.
PhylomeDBiP70259.
TreeFamiTF324849.

Family and domain databases

InterProiIPR001414. Ocular_alb1.
[Graphical view]
PfamiPF02101. Ocular_alb. 1 hit.
[Graphical view]
PRINTSiPR00965. OCULARALBNSM.

Sequencei

Sequence statusi: Complete.

P70259-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPRLGIFC CPTWDAATQL VLSFQPRVFH ALCLGSGTLR LVLGLLQLLS
60 70 80 90 100
GRRSVGHRAP ATSPAASVHI LRAATACDLL GCLGIVIRST VWIAYPEFIE
110 120 130 140 150
NISNVNATDI WPATFCVGSA MWIQLLYSAC FWWLFCYAVD VYLVIRRSAG
160 170 180 190 200
RSTILLYHIM AWGLAVLLCV EGAVMLYYPS VSRCERGLDH AIPHYVTTYL
210 220 230 240 250
PLLLVLVANP ILFHKTVTSV ASLLKGRKGV YTENERLMGA VIKTRFFKIM
260 270 280 290 300
LVLIACWLSN IINESLLFYL EMQPDIHGGS LKRIQNAART TWFIMGILNP
310 320 330 340 350
AQGLLLSLAF YGWTGCSLDV HPPKMVIQWE TMTASAAEGT YQTPVRSCVP
360 370 380 390 400
HQNPRKVVCV GGHTSDEVLS ILSEDSDAST VEIHTATGSC NIKEVDSISQ

AQGEL
Length:405
Mass (Da):44,564
Last modified:February 1, 1997 - v1
Checksum:i716405A6512ED312
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751M → S in AAC52890. (PubMed:8921399)Curated
Sequence conflicti213 – 2131F → L in AAC52890. (PubMed:8921399)Curated
Sequence conflicti285 – 2851Q → L in AAC52890. (PubMed:8921399)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98352 Genomic DNA. Translation: CAA66996.1.
U63918 mRNA. Translation: AAC52890.1.
CCDSiCCDS30476.1.
RefSeqiNP_035081.3. NM_010951.3.
UniGeneiMm.5157.

Genome annotation databases

EnsembliENSMUST00000026383; ENSMUSP00000026383; ENSMUSG00000025333.
GeneIDi18241.
KEGGimmu:18241.
UCSCiuc009uqo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98352 Genomic DNA. Translation: CAA66996.1 .
U63918 mRNA. Translation: AAC52890.1 .
CCDSi CCDS30476.1.
RefSeqi NP_035081.3. NM_010951.3.
UniGenei Mm.5157.

3D structure databases

ProteinModelPortali P70259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000026383.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P70259.

Proteomic databases

PRIDEi P70259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026383 ; ENSMUSP00000026383 ; ENSMUSG00000025333 .
GeneIDi 18241.
KEGGi mmu:18241.
UCSCi uc009uqo.1. mouse.

Organism-specific databases

CTDi 4935.
MGIi MGI:107193. Gpr143.

Phylogenomic databases

eggNOGi NOG39873.
HOGENOMi HOG000112769.
HOVERGENi HBG051784.
InParanoidi P70259.
KOi K08470.
OMAi MGILNPM.
OrthoDBi EOG7HXCQZ.
PhylomeDBi P70259.
TreeFami TF324849.

Miscellaneous databases

NextBioi 293676.
PROi P70259.
SOURCEi Search...

Gene expression databases

Bgeei P70259.
CleanExi MM_GPR143.
ExpressionAtlasi P70259. differential.
Genevestigatori P70259.

Family and domain databases

InterProi IPR001414. Ocular_alb1.
[Graphical view ]
Pfami PF02101. Ocular_alb. 1 hit.
[Graphical view ]
PRINTSi PR00965. OCULARALBNSM.
ProtoNeti Search...

Publicationsi

  1. "Cloning of the murine homolog of the ocular albinism type 1 (OA1) gene: sequence, genomic structure, and expression analysis in pigment cells."
    Bassi M.T., Incerti B., Easty D.J., Sviderskaya E.V., Ballabio A.
    Genome Res. 6:880-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Retina.
  2. "Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene."
    Newton J.M., Orlow S.J., Barsh G.S.
    Genomics 37:219-225(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells."
    Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., Schiaffino M.V.
    Hum. Mol. Genet. 17:3487-3501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGP143_MOUSE
AccessioniPrimary (citable) accession number: P70259
Secondary accession number(s): O89031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3