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P70259 (GP143_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 143
Alternative name(s):
MOA1
Ocular albinism type 1 protein homolog
Gene names
Name:Gpr143
Synonyms:Oa1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca2+ influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport. Ref.3

Subunit structure

Interacts with heterotrimeric G(i) proteins. Interacts with ARRB1 and ARRB2. Interacts with MLANA By similarity.

Subcellular location

Golgi apparatus By similarity. Melanosome membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity. Note: Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine By similarity.

Domain

The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells By similarity.

Post-translational modification

Glycosylated By similarity.

Phosphorylated By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor OA family.

Ontologies

Keywords
   Cellular componentCell membrane
Golgi apparatus
Lysosome
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-mediated signaling using intracellular calcium source

Inferred from sequence or structural similarity. Source: UniProtKB

melanosome localization

Inferred from mutant phenotype Ref.3. Source: UniProtKB

melanosome organization

Inferred from mutant phenotype Ref.3. Source: UniProtKB

melanosome transport

Inferred from mutant phenotype Ref.3. Source: UniProtKB

neuropeptide signaling pathway

Inferred from sequence or structural similarity. Source: GOC

phosphatidylinositol-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

apical plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosomal membrane

Inferred from sequence or structural similarity. Source: UniProtKB

melanosome

Inferred from sequence or structural similarity. Source: UniProtKB

melanosome membrane

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

L-DOPA binding

Inferred from sequence or structural similarity. Source: UniProtKB

L-DOPA receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

dopamine binding

Inferred from sequence or structural similarity. Source: UniProtKB

tyrosine binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405G-protein coupled receptor 143
PRO_0000195087

Regions

Topological domain1 – 2727Extracellular Potential
Transmembrane28 – 4821Helical; Name=1; Potential
Topological domain49 – 7830Cytoplasmic Potential
Transmembrane79 – 9921Helical; Name=2; Potential
Topological domain100 – 12425Extracellular Potential
Transmembrane125 – 14521Helical; Name=3; Potential
Topological domain146 – 1538Cytoplasmic Potential
Transmembrane154 – 17421Helical; Name=4; Potential
Topological domain175 – 19117Extracellular Potential
Transmembrane192 – 21221Helical; Name=5; Potential
Topological domain213 – 24836Cytoplasmic Potential
Transmembrane249 – 26921Helical; Name=6; Potential
Topological domain270 – 29223Extracellular Potential
Transmembrane293 – 31321Helical; Name=7; Potential
Topological domain314 – 40592Cytoplasmic Potential
Region221 – 23818Necessary for its G protein-activation ability and normal distribution of melanosomes By similarity
Motif222 – 23110lysosomal/melanosomal membrane localization signal By similarity
Motif329 – 3302lysosomal/melanosomal membrane localization signal By similarity

Amino acid modifications

Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1751M → S in AAC52890. Ref.2
Sequence conflict2131F → L in AAC52890. Ref.2
Sequence conflict2851Q → L in AAC52890. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P70259 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 716405A6512ED312

FASTA40544,564
        10         20         30         40         50         60 
MASPRLGIFC CPTWDAATQL VLSFQPRVFH ALCLGSGTLR LVLGLLQLLS GRRSVGHRAP 

        70         80         90        100        110        120 
ATSPAASVHI LRAATACDLL GCLGIVIRST VWIAYPEFIE NISNVNATDI WPATFCVGSA 

       130        140        150        160        170        180 
MWIQLLYSAC FWWLFCYAVD VYLVIRRSAG RSTILLYHIM AWGLAVLLCV EGAVMLYYPS 

       190        200        210        220        230        240 
VSRCERGLDH AIPHYVTTYL PLLLVLVANP ILFHKTVTSV ASLLKGRKGV YTENERLMGA 

       250        260        270        280        290        300 
VIKTRFFKIM LVLIACWLSN IINESLLFYL EMQPDIHGGS LKRIQNAART TWFIMGILNP 

       310        320        330        340        350        360 
AQGLLLSLAF YGWTGCSLDV HPPKMVIQWE TMTASAAEGT YQTPVRSCVP HQNPRKVVCV 

       370        380        390        400 
GGHTSDEVLS ILSEDSDAST VEIHTATGSC NIKEVDSISQ AQGEL 

« Hide

References

[1]"Cloning of the murine homolog of the ocular albinism type 1 (OA1) gene: sequence, genomic structure, and expression analysis in pigment cells."
Bassi M.T., Incerti B., Easty D.J., Sviderskaya E.V., Ballabio A.
Genome Res. 6:880-885(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Retina.
[2]"Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene."
Newton J.M., Orlow S.J., Barsh G.S.
Genomics 37:219-225(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells."
Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., Schiaffino M.V.
Hum. Mol. Genet. 17:3487-3501(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98352 Genomic DNA. Translation: CAA66996.1.
U63918 mRNA. Translation: AAC52890.1.
CCDSCCDS30476.1.
RefSeqNP_035081.3. NM_010951.3.
UniGeneMm.5157.

3D structure databases

ProteinModelPortalP70259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000026383.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP70259.

Proteomic databases

PRIDEP70259.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026383; ENSMUSP00000026383; ENSMUSG00000025333.
GeneID18241.
KEGGmmu:18241.
UCSCuc009uqo.1. mouse.

Organism-specific databases

CTD4935.
MGIMGI:107193. Gpr143.

Phylogenomic databases

eggNOGNOG39873.
HOGENOMHOG000112769.
HOVERGENHBG051784.
InParanoidP70259.
KOK08470.
OMAMGILNPM.
OrthoDBEOG7HXCQZ.
PhylomeDBP70259.
TreeFamTF324849.

Gene expression databases

BgeeP70259.
CleanExMM_GPR143.
GenevestigatorP70259.

Family and domain databases

InterProIPR001414. Ocular_alb1.
[Graphical view]
PfamPF02101. Ocular_alb. 1 hit.
[Graphical view]
PRINTSPR00965. OCULARALBNSM.
ProtoNetSearch...

Other

NextBio293676.
PROP70259.
SOURCESearch...

Entry information

Entry nameGP143_MOUSE
AccessionPrimary (citable) accession number: P70259
Secondary accession number(s): O89031
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries