P70259 (GP143_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 143

Alternative name(s):
MOA1
Ocular albinism type 1 protein homolog

Gene names

Name:	Gpr143
Synonyms:	Oa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca2+ influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport. Ref.3
Subunit structure	Interacts with heterotrimeric G(i) proteins. Interacts with ARRB1 and ARRB2. Interacts with MLANA By similarity.
Subcellular location	Golgi apparatus By similarity. Melanosome membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity. Note: Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine By similarity.
Domain	The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells By similarity.
Post-translational modification	Glycosylated By similarity. Phosphorylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor OA family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Lysosome Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium-mediated signaling using intracellular calcium source Inferred from sequence or structural similarity. Source: UniProtKB melanosome organization Inferred from mutant phenotype Ref.3. Source: UniProtKB melanosome transport Inferred from mutant phenotype Ref.3. Source: UniProtKB phosphatidylinositol-mediated signaling Inferred from sequence or structural similarity. Source: UniProtKB regulation of calcium-mediated signaling Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from sequence or structural similarity. Source: UniProtKB melanosome membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	L-DOPA binding Inferred from sequence or structural similarity. Source: UniProtKB L-DOPA receptor activity Inferred from sequence or structural similarity. Source: UniProtKB dopamine binding Inferred from sequence or structural similarity. Source: UniProtKB tyrosine binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	G-protein coupled receptor 143		PRO_0000195087
Regions
	Topological domain	1 – 27	27	Extracellular Potential
	Transmembrane	28 – 48	21	Helical; Name=1; Potential
	Topological domain	49 – 78	30	Cytoplasmic Potential
	Transmembrane	79 – 99	21	Helical; Name=2; Potential
	Topological domain	100 – 124	25	Extracellular Potential
	Transmembrane	125 – 145	21	Helical; Name=3; Potential
	Topological domain	146 – 153	8	Cytoplasmic Potential
	Transmembrane	154 – 174	21	Helical; Name=4; Potential
	Topological domain	175 – 191	17	Extracellular Potential
	Transmembrane	192 – 212	21	Helical; Name=5; Potential
	Topological domain	213 – 248	36	Cytoplasmic Potential
	Transmembrane	249 – 269	21	Helical; Name=6; Potential
	Topological domain	270 – 292	23	Extracellular Potential
	Transmembrane	293 – 313	21	Helical; Name=7; Potential
	Topological domain	314 – 405	92	Cytoplasmic Potential
	Region	221 – 238	18	Necessary for its G protein-activation ability and normal distribution of melanosomes By similarity
	Motif	222 – 231	10	lysosomal/melanosomal membrane localization signal By similarity
	Motif	329 – 330	2	lysosomal/melanosomal membrane localization signal By similarity
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	175	1	M → S in AAC52890. Ref.2
	Sequence conflict	213	1	F → L in AAC52890. Ref.2
	Sequence conflict	285	1	Q → L in AAC52890. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P70259 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 716405A6512ED312	FASTA	405	44,564
	10 20 30 40 50 60 MASPRLGIFC CPTWDAATQL VLSFQPRVFH ALCLGSGTLR LVLGLLQLLS GRRSVGHRAP 70 80 90 100 110 120 ATSPAASVHI LRAATACDLL GCLGIVIRST VWIAYPEFIE NISNVNATDI WPATFCVGSA 130 140 150 160 170 180 MWIQLLYSAC FWWLFCYAVD VYLVIRRSAG RSTILLYHIM AWGLAVLLCV EGAVMLYYPS 190 200 210 220 230 240 VSRCERGLDH AIPHYVTTYL PLLLVLVANP ILFHKTVTSV ASLLKGRKGV YTENERLMGA 250 260 270 280 290 300 VIKTRFFKIM LVLIACWLSN IINESLLFYL EMQPDIHGGS LKRIQNAART TWFIMGILNP 310 320 330 340 350 360 AQGLLLSLAF YGWTGCSLDV HPPKMVIQWE TMTASAAEGT YQTPVRSCVP HQNPRKVVCV 370 380 390 400 GGHTSDEVLS ILSEDSDAST VEIHTATGSC NIKEVDSISQ AQGEL

References

[1]	"Cloning of the murine homolog of the ocular albinism type 1 (OA1) gene: sequence, genomic structure, and expression analysis in pigment cells." Bassi M.T., Incerti B., Easty D.J., Sviderskaya E.V., Ballabio A. Genome Res. 6:880-885(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Retina.
[2]	"Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene." Newton J.M., Orlow S.J., Barsh G.S. Genomics 37:219-225(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells." Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., Schiaffino M.V. Hum. Mol. Genet. 17:3487-3501(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98352 Genomic DNA. Translation: CAA66996.1. U63918 mRNA. Translation: AAC52890.1.
IPI	IPI00137505.
RefSeq	NP_035081.3. NM_010951.3.
UniGene	Mm.5157.
3D structure databases
ProteinModelPortal	P70259.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000026383.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P70259.
Proteomic databases
PRIDE	P70259.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026383; ENSMUSP00000026383; ENSMUSG00000025333.
GeneID	18241.
KEGG	mmu:18241.
Organism-specific databases
CTD	4935.
MGI	MGI:107193. Gpr143.
Phylogenomic databases
eggNOG	NOG39873.
HOGENOM	HOG000112769.
HOVERGEN	HBG051784.
InParanoid	P70259.
KO	K08470.
OrthoDB	EOG42RD7J.
Gene expression databases
ArrayExpress	P70259.
Bgee	P70259.
CleanEx	MM_GPR143.
Genevestigator	P70259.
GermOnline	ENSMUSG00000025333. Mus musculus.
Family and domain databases
InterPro	IPR001414. Ocular_alb1. [Graphical view]
Pfam	PF02101. Ocular_alb. 1 hit. [Graphical view]
PRINTS	PR00965. OCULARALBNSM.
ProtoNet	Search...
Other
NextBio	293676.
SOURCE	Search...

Entry information

Entry name

GP143_MOUSE

Accession

Primary (citable) accession number: P70259
Secondary accession number(s): O89031

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program