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P70259

- GP143_MOUSE

UniProt

P70259 - GP143_MOUSE

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Protein
G-protein coupled receptor 143
Gene
Gpr143, Oa1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca2+ influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.1 Publication

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB
  2. L-DOPA binding Source: UniProtKB
  3. L-DOPA receptor activity Source: UniProtKB
  4. dopamine binding Source: UniProtKB
  5. tyrosine binding Source: UniProtKB

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: UniProtKB
  2. calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  3. melanosome localization Source: UniProtKB
  4. melanosome organization Source: UniProtKB
  5. melanosome transport Source: UniProtKB
  6. neuropeptide signaling pathway Source: GOC
  7. phosphatidylinositol-mediated signaling Source: UniProtKB
  8. regulation of calcium-mediated signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 143
Alternative name(s):
MOA1
Ocular albinism type 1 protein homolog
Gene namesi
Name:Gpr143
Synonyms:Oa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:107193. Gpr143.

Subcellular locationi

Golgi apparatus By similarity. Melanosome membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity
Note: Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727Extracellular Reviewed prediction
Add
BLAST
Transmembranei28 – 4821Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini49 – 7830Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei79 – 9921Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini100 – 12425Extracellular Reviewed prediction
Add
BLAST
Transmembranei125 – 14521Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini146 – 1538Cytoplasmic Reviewed prediction
Transmembranei154 – 17421Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini175 – 19117Extracellular Reviewed prediction
Add
BLAST
Transmembranei192 – 21221Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini213 – 24836Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei249 – 26921Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini270 – 29223Extracellular Reviewed prediction
Add
BLAST
Transmembranei293 – 31321Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini314 – 40592Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. apical plasma membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. lysosomal membrane Source: UniProtKB
  5. melanosome Source: UniProtKB
  6. melanosome membrane Source: UniProtKB
  7. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405G-protein coupled receptor 143
PRO_0000195087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi106 – 1061N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated By similarity.
Phosphorylated By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP70259.

PTM databases

PhosphoSiteiP70259.

Expressioni

Gene expression databases

BgeeiP70259.
CleanExiMM_GPR143.
GenevestigatoriP70259.

Interactioni

Subunit structurei

Interacts with heterotrimeric G(i) proteins. Interacts with ARRB1 and ARRB2. Interacts with MLANA By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026383.

Structurei

3D structure databases

ProteinModelPortaliP70259.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 23818Necessary for its G protein-activation ability and normal distribution of melanosomes By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi222 – 23110lysosomal/melanosomal membrane localization signal By similarity
Motifi329 – 3302lysosomal/melanosomal membrane localization signal By similarity

Domaini

The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39873.
HOGENOMiHOG000112769.
HOVERGENiHBG051784.
InParanoidiP70259.
KOiK08470.
OMAiMGILNPM.
OrthoDBiEOG7HXCQZ.
PhylomeDBiP70259.
TreeFamiTF324849.

Family and domain databases

InterProiIPR001414. Ocular_alb1.
[Graphical view]
PfamiPF02101. Ocular_alb. 1 hit.
[Graphical view]
PRINTSiPR00965. OCULARALBNSM.

Sequencei

Sequence statusi: Complete.

P70259-1 [UniParc]FASTAAdd to Basket

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MASPRLGIFC CPTWDAATQL VLSFQPRVFH ALCLGSGTLR LVLGLLQLLS    50
GRRSVGHRAP ATSPAASVHI LRAATACDLL GCLGIVIRST VWIAYPEFIE 100
NISNVNATDI WPATFCVGSA MWIQLLYSAC FWWLFCYAVD VYLVIRRSAG 150
RSTILLYHIM AWGLAVLLCV EGAVMLYYPS VSRCERGLDH AIPHYVTTYL 200
PLLLVLVANP ILFHKTVTSV ASLLKGRKGV YTENERLMGA VIKTRFFKIM 250
LVLIACWLSN IINESLLFYL EMQPDIHGGS LKRIQNAART TWFIMGILNP 300
AQGLLLSLAF YGWTGCSLDV HPPKMVIQWE TMTASAAEGT YQTPVRSCVP 350
HQNPRKVVCV GGHTSDEVLS ILSEDSDAST VEIHTATGSC NIKEVDSISQ 400
AQGEL 405
Length:405
Mass (Da):44,564
Last modified:February 1, 1997 - v1
Checksum:i716405A6512ED312
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751M → S in AAC52890. 1 Publication
Sequence conflicti213 – 2131F → L in AAC52890. 1 Publication
Sequence conflicti285 – 2851Q → L in AAC52890. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98352 Genomic DNA. Translation: CAA66996.1.
U63918 mRNA. Translation: AAC52890.1.
CCDSiCCDS30476.1.
RefSeqiNP_035081.3. NM_010951.3.
UniGeneiMm.5157.

Genome annotation databases

EnsembliENSMUST00000026383; ENSMUSP00000026383; ENSMUSG00000025333.
GeneIDi18241.
KEGGimmu:18241.
UCSCiuc009uqo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98352 Genomic DNA. Translation: CAA66996.1 .
U63918 mRNA. Translation: AAC52890.1 .
CCDSi CCDS30476.1.
RefSeqi NP_035081.3. NM_010951.3.
UniGenei Mm.5157.

3D structure databases

ProteinModelPortali P70259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000026383.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P70259.

Proteomic databases

PRIDEi P70259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026383 ; ENSMUSP00000026383 ; ENSMUSG00000025333 .
GeneIDi 18241.
KEGGi mmu:18241.
UCSCi uc009uqo.1. mouse.

Organism-specific databases

CTDi 4935.
MGIi MGI:107193. Gpr143.

Phylogenomic databases

eggNOGi NOG39873.
HOGENOMi HOG000112769.
HOVERGENi HBG051784.
InParanoidi P70259.
KOi K08470.
OMAi MGILNPM.
OrthoDBi EOG7HXCQZ.
PhylomeDBi P70259.
TreeFami TF324849.

Miscellaneous databases

NextBioi 293676.
PROi P70259.
SOURCEi Search...

Gene expression databases

Bgeei P70259.
CleanExi MM_GPR143.
Genevestigatori P70259.

Family and domain databases

InterProi IPR001414. Ocular_alb1.
[Graphical view ]
Pfami PF02101. Ocular_alb. 1 hit.
[Graphical view ]
PRINTSi PR00965. OCULARALBNSM.
ProtoNeti Search...

Publicationsi

  1. "Cloning of the murine homolog of the ocular albinism type 1 (OA1) gene: sequence, genomic structure, and expression analysis in pigment cells."
    Bassi M.T., Incerti B., Easty D.J., Sviderskaya E.V., Ballabio A.
    Genome Res. 6:880-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Retina.
  2. "Isolation and characterization of a mouse homolog of the X-linked ocular albinism (OA1) gene."
    Newton J.M., Orlow S.J., Barsh G.S.
    Genomics 37:219-225(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells."
    Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., Schiaffino M.V.
    Hum. Mol. Genet. 17:3487-3501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGP143_MOUSE
AccessioniPrimary (citable) accession number: P70259
Secondary accession number(s): O89031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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