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P70257 (NFIX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor 1 X-type

Short name=NF1-X
Short name=Nuclear factor 1/X
Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/X
Short name=NF-I/X
Short name=NFI-X
TGGCA-binding protein
Gene names
Name:Nfix
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor. Ref.1

Subunit structure

Binds DNA as a homodimer.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the CTF/NF-I family.

Contains 1 CTF/NF-I DNA-binding domain.

Ontologies

Keywords
   Biological processDNA replication
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
Repressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

astrocyte differentiation

Inferred from mutant phenotype PubMed 21800304. Source: MGI

cerebellar Purkinje cell layer development

Inferred from mutant phenotype PubMed 21800304. Source: MGI

cerebellar granule cell differentiation

Inferred from mutant phenotype PubMed 21800304. Source: MGI

cerebellum development

Inferred from mutant phenotype PubMed 21800304. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19706729. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 21800304Ref.4. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 20178747. Source: IntAct

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform NFIX3 (identifier: P70257-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform NFIX1 (identifier: P70257-1)

The sequence of this isoform differs from the canonical sequence as follows:
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.
Isoform NFIX2 (identifier: P70257-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-360: Missing.
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Nuclear factor 1 X-type
PRO_0000100205

Regions

DNA binding1 – 194194CTF/NF-I

Amino acid modifications

Modified residue2801Phosphoserine Ref.6 Ref.7
Modified residue2881Phosphoserine Ref.6
Modified residue3011Phosphoserine Ref.7
Modified residue3411Phosphoserine Ref.7

Natural variations

Alternative sequence320 – 36041Missing in isoform NFIX2.
VSP_003563
Alternative sequence419 – 44123PNGSG…PPVAR → HSQRQAPPLPTGLSASDPGT ATF in isoform NFIX1 and isoform NFIX2.
VSP_007546
Alternative sequence442 – 48847Missing in isoform NFIX1 and isoform NFIX2.
VSP_007547

Experimental info

Sequence conflict3531V → L in CAA68952. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform NFIX3 [UniParc].

Last modified June 16, 2003. Version 2.
Checksum: 157F67EC17C96AD0

FASTA48853,394
        10         20         30         40         50         60 
MYSPYCLTQD EFHPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSK DEERAVKDEL 

        70         80         90        100        110        120 
LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKP PCCVLSNPDQ KGKIRRIDCL 

       130        140        150        160        170        180 
RQADKVWRLD LVMVILFKGI PLESTDGERL YKSPQCSNPG LCVQPHHIGV TIKELDLYLA 

       190        200        210        220        230        240 
YFVHTPESGQ SDSSNQQGDA DIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG 

       250        260        270        280        290        300 
PNFSLADLES PSYYNINQVT LGRRSITSPP STSSTKRPKS IDDSEMESPV DDVFYPGTGR 

       310        320        330        340        350        360 
SPAAGSSQSS GWPNDVDAGP ASLKKSGKLD FCSALSSQGS SPRMAFTHHP LPVLAGVRPG 

       370        380        390        400        410        420 
SPRATASALH FPSTSIIQQS SPYFTHPTIR YHHHHGQDSL KEFVQFVCSD GSGQATGQPN 

       430        440        450        460        470        480 
GSGQGKVPGS FLLPPPPPVA RPVPLPMPDS KTTSTAPDGA ALTPPSPSFT TTGASSANRF 


VGIGPRDG 

« Hide

Isoform NFIX1 [UniParc].

Checksum: EB46ABAD96CE9E80
Show »

FASTA44148,853
Isoform NFIX2 [UniParc].

Checksum: 4E89D0236A3F7235
Show »

FASTA40044,637

References

« Hide 'large scale' references
[1]"NFI/X proteins: a class of NFI family of transcription factors with positive and negative regulatory domains."
Nebl G., Cato A.C.B.
Cell. Mol. Biol. Res. 41:85-95(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS NFIX1; NFIX2 AND NFIX3), FUNCTION.
[2]"Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS NFIX1 AND NFIX2).
Strain: NIH Swiss.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NFIX1).
Tissue: Mammary gland.
[4]"Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-488 (ISOFORM NFIX2).
Strain: BALB/c.
[5]"Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-186.
Strain: 129.
[6]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280; SER-301 AND SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S81451 mRNA. Translation: AAB36083.2.
Y07688 mRNA. Translation: CAA68952.1.
Y07689 mRNA. Translation: CAA68953.1.
BC003766 mRNA. Translation: AAH03766.1.
U57636 mRNA. Translation: AAB49931.1.
AF111266 Genomic DNA. Translation: AAD39101.1.
CCDSCCDS40412.1. [P70257-2]
CCDS40413.1. [P70257-1]
RefSeqNP_001075451.1. NM_001081982.2. [P70257-1]
NP_035036.1. NM_010906.3. [P70257-2]
UniGeneMm.487058.
Mm.9394.

3D structure databases

ProteinModelPortalP70257.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP70257. 4 interactions.
MINTMINT-4103732.

PTM databases

PhosphoSiteP70257.

Proteomic databases

PaxDbP70257.
PRIDEP70257.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911. [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911. [P70257-1]
GeneID18032.
KEGGmmu:18032.
UCSCuc009mne.1. mouse. [P70257-1]
uc009mnf.1. mouse. [P70257-2]

Organism-specific databases

CTD4784.
MGIMGI:97311. Nfix.

Phylogenomic databases

eggNOGNOG252172.
GeneTreeENSGT00390000009905.
HOGENOMHOG000013028.
HOVERGENHBG006561.
KOK09171.
PhylomeDBP70257.

Gene expression databases

ArrayExpressP70257.
BgeeP70257.
CleanExMM_NFIX.
GenevestigatorP70257.

Family and domain databases

InterProIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERPTHR11492. PTHR11492. 1 hit.
PfamPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTSM00523. DWA. 1 hit.
[Graphical view]
PROSITEPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293113.
PROP70257.
SOURCESearch...

Entry information

Entry nameNFIX_MOUSE
AccessionPrimary (citable) accession number: P70257
Secondary accession number(s): O08519 expand/collapse secondary AC list , P70258, Q64192, Q99L78, Q9R1G2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2003
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot