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Protein

Nuclear factor 1 X-type

Gene

Nfix

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 194CTF/NF-IPROSITE-ProRule annotationAdd BLAST194

GO - Molecular functioni

GO - Biological processi

  • astrocyte differentiation Source: MGI
  • cerebellar granule cell differentiation Source: MGI
  • cerebellar Purkinje cell layer development Source: MGI
  • cerebellum development Source: MGI
  • DNA replication Source: UniProtKB-KW
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 X-type
Short name:
NF1-X
Short name:
Nuclear factor 1/X
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/X
Short name:
NF-I/X
Short name:
NFI-X
TGGCA-binding protein
Gene namesi
Name:Nfix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97311. Nfix.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002051 – 488Nuclear factor 1 X-typeAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265PhosphoserineCombined sources1
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei280PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei341PhosphoserineCombined sources1
Modified residuei343Asymmetric dimethylarginineCombined sources1
Modified residuei390Asymmetric dimethylarginineCombined sources1
Isoform NFIX2 (identifier: P70257-2)
Modified residuei301PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP70257.
PeptideAtlasiP70257.
PRIDEiP70257.

PTM databases

iPTMnetiP70257.
PhosphoSitePlusiP70257.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001911.
CleanExiMM_NFIX.
ExpressionAtlasiP70257. baseline and differential.
GenevisibleiP70257. MM.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi201750. 1 interactor.
IntActiP70257. 5 interactors.
MINTiMINT-4103732.
STRINGi10090.ENSMUSP00000105386.

Structurei

3D structure databases

ProteinModelPortaliP70257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3663. Eukaryota.
ENOG410Y16G. LUCA.
GeneTreeiENSGT00390000009905.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP70257.
KOiK09171.
PhylomeDBiP70257.

Family and domain databases

InterProiView protein in InterPro
IPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiView protein in Pfam
PF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
SMARTiView protein in SMART
SM00523. DWA. 1 hit.
PROSITEiView protein in PROSITE
PS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform NFIX3 (identifier: P70257-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSPYCLTQD EFHPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSK
60 70 80 90 100
DEERAVKDEL LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKP
110 120 130 140 150
PCCVLSNPDQ KGKIRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL
160 170 180 190 200
YKSPQCSNPG LCVQPHHIGV TIKELDLYLA YFVHTPESGQ SDSSNQQGDA
210 220 230 240 250
DIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG PNFSLADLES
260 270 280 290 300
PSYYNINQVT LGRRSITSPP STSSTKRPKS IDDSEMESPV DDVFYPGTGR
310 320 330 340 350
SPAAGSSQSS GWPNDVDAGP ASLKKSGKLD FCSALSSQGS SPRMAFTHHP
360 370 380 390 400
LPVLAGVRPG SPRATASALH FPSTSIIQQS SPYFTHPTIR YHHHHGQDSL
410 420 430 440 450
KEFVQFVCSD GSGQATGQPN GSGQGKVPGS FLLPPPPPVA RPVPLPMPDS
460 470 480
KTTSTAPDGA ALTPPSPSFT TTGASSANRF VGIGPRDG
Length:488
Mass (Da):53,394
Last modified:June 16, 2003 - v2
Checksum:i157F67EC17C96AD0
GO
Isoform NFIX1 (identifier: P70257-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:441
Mass (Da):48,853
Checksum:iEB46ABAD96CE9E80
GO
Isoform NFIX2 (identifier: P70257-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-360: Missing.
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:400
Mass (Da):44,637
Checksum:i4E89D0236A3F7235
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353V → L in CAA68952 (PubMed:12568726).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003563320 – 360Missing in isoform NFIX2. 3 PublicationsAdd BLAST41
Alternative sequenceiVSP_007546419 – 441PNGSG…PPVAR → HSQRQAPPLPTGLSASDPGT ATF in isoform NFIX1 and isoform NFIX2. 4 PublicationsAdd BLAST23
Alternative sequenceiVSP_007547442 – 488Missing in isoform NFIX1 and isoform NFIX2. 4 PublicationsAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81451 mRNA. Translation: AAB36083.2.
Y07688 mRNA. Translation: CAA68952.1.
Y07689 mRNA. Translation: CAA68953.1.
BC003766 mRNA. Translation: AAH03766.1.
U57636 mRNA. Translation: AAB49931.1.
AF111266 Genomic DNA. Translation: AAD39101.1.
CCDSiCCDS40412.1. [P70257-2]
CCDS40413.1. [P70257-1]
RefSeqiNP_001075451.1. NM_001081982.2. [P70257-1]
NP_035036.1. NM_010906.3. [P70257-2]
UniGeneiMm.487058.
Mm.9394.

Genome annotation databases

EnsembliENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911. [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911. [P70257-1]
GeneIDi18032.
KEGGimmu:18032.
UCSCiuc009mne.2. mouse. [P70257-1]
uc009mnf.2. mouse. [P70257-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNFIX_MOUSE
AccessioniPrimary (citable) accession number: P70257
Secondary accession number(s): O08519
, P70258, Q64192, Q99L78, Q9R1G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2003
Last modified: August 30, 2017
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families