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Protein

Nuclear factor 1 X-type

Gene

Nfix

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1 – 194194CTF/NF-IPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. astrocyte differentiation Source: MGI
  2. cerebellar granule cell differentiation Source: MGI
  3. cerebellar Purkinje cell layer development Source: MGI
  4. cerebellum development Source: MGI
  5. DNA replication Source: UniProtKB-KW
  6. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_285756. RNA Polymerase III Transcription Termination.
REACT_294024. RNA Polymerase III Abortive And Retractive Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 X-type
Short name:
NF1-X
Short name:
Nuclear factor 1/X
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/X
Short name:
NF-I/X
Short name:
NFI-X
TGGCA-binding protein
Gene namesi
Name:Nfix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97311. Nfix.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Nuclear factor 1 X-typePRO_0000100205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei280 – 2801Phosphoserine2 Publications
Modified residuei288 – 2881Phosphoserine1 Publication
Modified residuei301 – 3011Phosphoserine1 Publication
Modified residuei341 – 3411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70257.
PaxDbiP70257.
PRIDEiP70257.

PTM databases

PhosphoSiteiP70257.

Expressioni

Gene expression databases

BgeeiP70257.
CleanExiMM_NFIX.
ExpressionAtlasiP70257. baseline and differential.
GenevestigatoriP70257.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Mef2aQ609292EBI-2639084,EBI-2639094
PrkcqQ021113EBI-2639084,EBI-2639157

Protein-protein interaction databases

IntActiP70257. 4 interactions.
MINTiMINT-4103732.

Structurei

3D structure databases

ProteinModelPortaliP70257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation
Contains 1 CTF/NF-I DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG252172.
GeneTreeiENSGT00390000009905.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP70257.
KOiK09171.
PhylomeDBiP70257.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform NFIX3 (identifier: P70257-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSPYCLTQD EFHPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSK
60 70 80 90 100
DEERAVKDEL LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKP
110 120 130 140 150
PCCVLSNPDQ KGKIRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL
160 170 180 190 200
YKSPQCSNPG LCVQPHHIGV TIKELDLYLA YFVHTPESGQ SDSSNQQGDA
210 220 230 240 250
DIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG PNFSLADLES
260 270 280 290 300
PSYYNINQVT LGRRSITSPP STSSTKRPKS IDDSEMESPV DDVFYPGTGR
310 320 330 340 350
SPAAGSSQSS GWPNDVDAGP ASLKKSGKLD FCSALSSQGS SPRMAFTHHP
360 370 380 390 400
LPVLAGVRPG SPRATASALH FPSTSIIQQS SPYFTHPTIR YHHHHGQDSL
410 420 430 440 450
KEFVQFVCSD GSGQATGQPN GSGQGKVPGS FLLPPPPPVA RPVPLPMPDS
460 470 480
KTTSTAPDGA ALTPPSPSFT TTGASSANRF VGIGPRDG
Length:488
Mass (Da):53,394
Last modified:June 15, 2003 - v2
Checksum:i157F67EC17C96AD0
GO
Isoform NFIX1 (identifier: P70257-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:441
Mass (Da):48,853
Checksum:iEB46ABAD96CE9E80
GO
Isoform NFIX2 (identifier: P70257-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-360: Missing.
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:400
Mass (Da):44,637
Checksum:i4E89D0236A3F7235
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531V → L in CAA68952 (PubMed:12568726).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei320 – 36041Missing in isoform NFIX2. 3 PublicationsVSP_003563Add
BLAST
Alternative sequencei419 – 44123PNGSG…PPVAR → HSQRQAPPLPTGLSASDPGT ATF in isoform NFIX1 and isoform NFIX2. 4 PublicationsVSP_007546Add
BLAST
Alternative sequencei442 – 48847Missing in isoform NFIX1 and isoform NFIX2. 4 PublicationsVSP_007547Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81451 mRNA. Translation: AAB36083.2.
Y07688 mRNA. Translation: CAA68952.1.
Y07689 mRNA. Translation: CAA68953.1.
BC003766 mRNA. Translation: AAH03766.1.
U57636 mRNA. Translation: AAB49931.1.
AF111266 Genomic DNA. Translation: AAD39101.1.
CCDSiCCDS40412.1. [P70257-2]
CCDS40413.1. [P70257-1]
RefSeqiNP_001075451.1. NM_001081982.2. [P70257-1]
NP_035036.1. NM_010906.3. [P70257-2]
UniGeneiMm.487058.
Mm.9394.

Genome annotation databases

EnsembliENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911. [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911. [P70257-1]
GeneIDi18032.
KEGGimmu:18032.
UCSCiuc009mne.1. mouse. [P70257-1]
uc009mnf.1. mouse. [P70257-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81451 mRNA. Translation: AAB36083.2.
Y07688 mRNA. Translation: CAA68952.1.
Y07689 mRNA. Translation: CAA68953.1.
BC003766 mRNA. Translation: AAH03766.1.
U57636 mRNA. Translation: AAB49931.1.
AF111266 Genomic DNA. Translation: AAD39101.1.
CCDSiCCDS40412.1. [P70257-2]
CCDS40413.1. [P70257-1]
RefSeqiNP_001075451.1. NM_001081982.2. [P70257-1]
NP_035036.1. NM_010906.3. [P70257-2]
UniGeneiMm.487058.
Mm.9394.

3D structure databases

ProteinModelPortaliP70257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP70257. 4 interactions.
MINTiMINT-4103732.

PTM databases

PhosphoSiteiP70257.

Proteomic databases

MaxQBiP70257.
PaxDbiP70257.
PRIDEiP70257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911. [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911. [P70257-1]
GeneIDi18032.
KEGGimmu:18032.
UCSCiuc009mne.1. mouse. [P70257-1]
uc009mnf.1. mouse. [P70257-2]

Organism-specific databases

CTDi4784.
MGIiMGI:97311. Nfix.

Phylogenomic databases

eggNOGiNOG252172.
GeneTreeiENSGT00390000009905.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP70257.
KOiK09171.
PhylomeDBiP70257.

Enzyme and pathway databases

ReactomeiREACT_285756. RNA Polymerase III Transcription Termination.
REACT_294024. RNA Polymerase III Abortive And Retractive Initiation.

Miscellaneous databases

NextBioi293113.
PROiP70257.
SOURCEiSearch...

Gene expression databases

BgeeiP70257.
CleanExiMM_NFIX.
ExpressionAtlasiP70257. baseline and differential.
GenevestigatoriP70257.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NFI/X proteins: a class of NFI family of transcription factors with positive and negative regulatory domains."
    Nebl G., Cato A.C.B.
    Cell. Mol. Biol. Res. 41:85-95(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS NFIX1; NFIX2 AND NFIX3), FUNCTION.
  2. "Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
    Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
    Gene 304:171-181(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS NFIX1 AND NFIX2).
    Strain: NIH Swiss.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NFIX1).
    Tissue: Mammary gland.
  4. "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
    Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
    Dev. Dyn. 208:313-325(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-488 (ISOFORM NFIX2).
    Strain: BALB/c.
  5. "Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
    Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
    Mamm. Genome 10:390-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-186.
    Strain: 129.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280; SER-301 AND SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNFIX_MOUSE
AccessioniPrimary (citable) accession number: P70257
Secondary accession number(s): O08519
, P70258, Q64192, Q99L78, Q9R1G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: June 15, 2003
Last modified: March 31, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.