Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P70255

- NFIC_MOUSE

UniProt

P70255 - NFIC_MOUSE

Protein

Nuclear factor 1 C-type

Gene

Nfic

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi1 – 195195CTF/NF-IPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter proximal region DNA binding Source: Ensembl
    2. double-stranded DNA binding Source: Ensembl
    3. sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    3. odontogenesis of dentin-containing tooth Source: MGI
    4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    DNA replication, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_221403. RNA Polymerase III Transcription Termination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear factor 1 C-type
    Short name:
    NF1-C
    Short name:
    Nuclear factor 1/C
    Alternative name(s):
    CCAAT-box-binding transcription factor
    Short name:
    CTF
    Nuclear factor I/C
    Short name:
    NF-I/C
    Short name:
    NFI-C
    TGGCA-binding protein
    Gene namesi
    Name:Nfic
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:109591. Nfic.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 439439Nuclear factor 1 C-typePRO_0000100200Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei194 – 1941PhosphoserineBy similarity
    Modified residuei294 – 2941PhosphoserineBy similarity
    Modified residuei323 – 3231Phosphoserine1 Publication
    Modified residuei333 – 3331Phosphoserine1 Publication
    Modified residuei337 – 3371PhosphoserineBy similarity
    Modified residuei339 – 3391Phosphoserine2 Publications
    Modified residuei343 – 3431Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP70255.
    PRIDEiP70255.

    PTM databases

    PhosphoSiteiP70255.

    Expressioni

    Tissue specificityi

    Highest levels in skeletal muscle. Lower levels in heart, liver, kidney, lung and brain. Very low levels in testis and spleen.

    Gene expression databases

    ArrayExpressiP70255.
    BgeeiP70255.
    CleanExiMM_NFIC.
    GenevestigatoriP70255.

    Interactioni

    Subunit structurei

    Binds DNA as a homodimer.

    Protein-protein interaction databases

    BioGridi201748. 1 interaction.
    IntActiP70255. 1 interaction.
    MINTiMINT-4103664.

    Structurei

    3D structure databases

    ProteinModelPortaliP70255.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CTF/NF-I family.PROSITE-ProRule annotation
    Contains 1 CTF/NF-I DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG252172.
    GeneTreeiENSGT00390000009905.
    HOVERGENiHBG006561.
    InParanoidiP70255.
    KOiK09170.
    OMAiMEGGISP.
    OrthoDBiEOG7WDN2K.
    PhylomeDBiP70255.
    TreeFamiTF313889.

    Family and domain databases

    InterProiIPR000647. CTF/NFI.
    IPR020604. CTF/NFI_DNA-bd-dom.
    IPR019739. CTF/NFI_DNA-bd_CS.
    IPR019548. CTF/NFI_DNA-bd_N.
    IPR003619. MAD_homology1_Dwarfin-type.
    [Graphical view]
    PANTHERiPTHR11492. PTHR11492. 1 hit.
    PfamiPF00859. CTF_NFI. 1 hit.
    PF03165. MH1. 1 hit.
    PF10524. NfI_DNAbd_pre-N. 1 hit.
    [Graphical view]
    SMARTiSM00523. DWA. 1 hit.
    [Graphical view]
    PROSITEiPS00349. CTF_NFI_1. 1 hit.
    PS51080. CTF_NFI_2. 1 hit.
    [Graphical view]

    Sequences (7)i

    Sequence statusi: Complete.

    This entry describes 7 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P70255-1) [UniParc]FASTAAdd to Basket

    Also known as: C1A, C2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS    50
    KDEERAVKDE LLGEKAEVKQ KWASRLLAKL RKDIRPECRE DFVLAVTGKK 100
    APGCVLSNPD QKGKMRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER 150
    LVKAAACAHP VLCVQPHHIG VAVKELDLYL AYFVRERDAE QSSSPRTGVG 200
    SDQEDSKPIT LDTTDFQESF VTSGVFSVTE LIQVSRTPVV TGTGPNFSLG 250
    ELQGHLAYDL NPASAGMRRT LPSTSSSGSK RHKSGSMEED VDTSPGGDYY 300
    TSPNSPTSSS RNWTEDIEGG ISSPVKKTEM DKSPFNSPSP QDSPRLSSFT 350
    QHHRPVIAVH SGIARSPHPT SALHFPATPI LPQTASTYFP HTAIRYPPHL 400
    NPQDPLKDLV SLACDPATQQ PGPPALRPTR PLQTVPLWD 439
    Length:439
    Mass (Da):48,768
    Last modified:February 1, 1997 - v1
    Checksum:iF794131A7A1B686A
    GO
    Isoform 2 (identifier: P70255-2) [UniParc]FASTAAdd to Basket

    Also known as: C1B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-10: MYSSPLCLTQ → M

    Show »
    Length:430
    Mass (Da):47,775
    Checksum:i348C76F604357548
    GO
    Isoform 3 (identifier: P70255-3) [UniParc]FASTAAdd to Basket

    Also known as: C5

    The sequence of this isoform differs from the canonical sequence as follows:
         424-439: PALRPTRPLQTVPLWD → SWYLG

    Show »
    Length:428
    Mass (Da):47,533
    Checksum:i6B9FD2D21C7AC136
    GO
    Isoform 4 (identifier: P70255-4) [UniParc]FASTAAdd to Basket

    Also known as: C8

    The sequence of this isoform differs from the canonical sequence as follows:
         320-361: Missing.

    Show »
    Length:397
    Mass (Da):44,171
    Checksum:iE66D569D32A5AD99
    GO
    Isoform 5 (identifier: P70255-5) [UniParc]FASTAAdd to Basket

    Also known as: C9

    The sequence of this isoform differs from the canonical sequence as follows:
         362-423: Missing.

    Show »
    Length:377
    Mass (Da):42,137
    Checksum:iD6812CA58974C641
    GO
    Isoform 6 (identifier: P70255-6) [UniParc]FASTAAdd to Basket

    Also known as: C10

    The sequence of this isoform differs from the canonical sequence as follows:
         247-374: Missing.

    Show »
    Length:311
    Mass (Da):35,067
    Checksum:i546844CFEB48DABF
    GO
    Isoform 7 (identifier: P70255-7) [UniParc]FASTAAdd to Basket

    Also known as: C11

    The sequence of this isoform differs from the canonical sequence as follows:
         247-374: Missing.
         424-439: PALRPTRPLQTVPLWD → SWYLG

    Show »
    Length:300
    Mass (Da):33,832
    Checksum:i86D75E67C14D2ECF
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1010MYSSPLCLTQ → M in isoform 2. 1 PublicationVSP_003557
    Alternative sequencei247 – 374128Missing in isoform 6 and isoform 7. CuratedVSP_007556Add
    BLAST
    Alternative sequencei320 – 36142Missing in isoform 4. CuratedVSP_007557Add
    BLAST
    Alternative sequencei362 – 42362Missing in isoform 5. CuratedVSP_007558Add
    BLAST
    Alternative sequencei424 – 43916PALRP…VPLWD → SWYLG in isoform 3 and isoform 7. CuratedVSP_007559Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y07692 mRNA. Translation: CAA68956.1.
    Y07693 mRNA. Translation: CAA68957.1.
    U57635 mRNA. Translation: AAB49930.1.
    AK087539 mRNA. Translation: BAC39918.1.
    AK155258 mRNA. Translation: BAE33151.1.
    AF358455 mRNA. Translation: AAK21331.1.
    AF358456 mRNA. Translation: AAK21332.1.
    AF358457 mRNA. Translation: AAK21333.1.
    AF358458 mRNA. Translation: AAK21334.1.
    AF358459 mRNA. Translation: AAK21335.1.
    AF358460 mRNA. Translation: AAK21336.1.
    AF111265 Genomic DNA. Translation: AAD39100.1.
    CCDSiCCDS24058.1. [P70255-1]
    CCDS35999.1. [P70255-3]
    RefSeqiNP_032714.1. NM_008688.3. [P70255-1]
    XP_006513398.1. XM_006513335.1. [P70255-2]
    UniGeneiMm.426936.
    Mm.453078.

    Genome annotation databases

    EnsembliENSMUST00000020461; ENSMUSP00000020461; ENSMUSG00000055053. [P70255-1]
    ENSMUST00000078185; ENSMUSP00000077317; ENSMUSG00000055053. [P70255-4]
    ENSMUST00000105321; ENSMUSP00000100958; ENSMUSG00000055053. [P70255-3]
    ENSMUST00000117966; ENSMUSP00000113046; ENSMUSG00000055053. [P70255-2]
    GeneIDi18029.
    KEGGimmu:18029.
    UCSCiuc007ghx.1. mouse. [P70255-4]
    uc007ghy.1. mouse. [P70255-1]
    uc007gia.1. mouse. [P70255-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y07692 mRNA. Translation: CAA68956.1 .
    Y07693 mRNA. Translation: CAA68957.1 .
    U57635 mRNA. Translation: AAB49930.1 .
    AK087539 mRNA. Translation: BAC39918.1 .
    AK155258 mRNA. Translation: BAE33151.1 .
    AF358455 mRNA. Translation: AAK21331.1 .
    AF358456 mRNA. Translation: AAK21332.1 .
    AF358457 mRNA. Translation: AAK21333.1 .
    AF358458 mRNA. Translation: AAK21334.1 .
    AF358459 mRNA. Translation: AAK21335.1 .
    AF358460 mRNA. Translation: AAK21336.1 .
    AF111265 Genomic DNA. Translation: AAD39100.1 .
    CCDSi CCDS24058.1. [P70255-1 ]
    CCDS35999.1. [P70255-3 ]
    RefSeqi NP_032714.1. NM_008688.3. [P70255-1 ]
    XP_006513398.1. XM_006513335.1. [P70255-2 ]
    UniGenei Mm.426936.
    Mm.453078.

    3D structure databases

    ProteinModelPortali P70255.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201748. 1 interaction.
    IntActi P70255. 1 interaction.
    MINTi MINT-4103664.

    PTM databases

    PhosphoSitei P70255.

    Proteomic databases

    PaxDbi P70255.
    PRIDEi P70255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020461 ; ENSMUSP00000020461 ; ENSMUSG00000055053 . [P70255-1 ]
    ENSMUST00000078185 ; ENSMUSP00000077317 ; ENSMUSG00000055053 . [P70255-4 ]
    ENSMUST00000105321 ; ENSMUSP00000100958 ; ENSMUSG00000055053 . [P70255-3 ]
    ENSMUST00000117966 ; ENSMUSP00000113046 ; ENSMUSG00000055053 . [P70255-2 ]
    GeneIDi 18029.
    KEGGi mmu:18029.
    UCSCi uc007ghx.1. mouse. [P70255-4 ]
    uc007ghy.1. mouse. [P70255-1 ]
    uc007gia.1. mouse. [P70255-2 ]

    Organism-specific databases

    CTDi 4782.
    MGIi MGI:109591. Nfic.

    Phylogenomic databases

    eggNOGi NOG252172.
    GeneTreei ENSGT00390000009905.
    HOVERGENi HBG006561.
    InParanoidi P70255.
    KOi K09170.
    OMAi MEGGISP.
    OrthoDBi EOG7WDN2K.
    PhylomeDBi P70255.
    TreeFami TF313889.

    Enzyme and pathway databases

    Reactomei REACT_221403. RNA Polymerase III Transcription Termination.

    Miscellaneous databases

    ChiTaRSi NFIC. mouse.
    NextBioi 293103.
    PROi P70255.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70255.
    Bgeei P70255.
    CleanExi MM_NFIC.
    Genevestigatori P70255.

    Family and domain databases

    InterProi IPR000647. CTF/NFI.
    IPR020604. CTF/NFI_DNA-bd-dom.
    IPR019739. CTF/NFI_DNA-bd_CS.
    IPR019548. CTF/NFI_DNA-bd_N.
    IPR003619. MAD_homology1_Dwarfin-type.
    [Graphical view ]
    PANTHERi PTHR11492. PTHR11492. 1 hit.
    Pfami PF00859. CTF_NFI. 1 hit.
    PF03165. MH1. 1 hit.
    PF10524. NfI_DNAbd_pre-N. 1 hit.
    [Graphical view ]
    SMARTi SM00523. DWA. 1 hit.
    [Graphical view ]
    PROSITEi PS00349. CTF_NFI_1. 1 hit.
    PS51080. CTF_NFI_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
      Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
      Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Strain: NIH Swiss.
    2. "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
      Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
      Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Liver and Skeletal muscle.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Eye.
    4. Kane R., Martin F.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 179-439 (ISOFORMS 1; 3; 4; 5; 6 AND 7).
      Strain: CD-1.
      Tissue: Mammary gland.
    5. "Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
      Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
      Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 11-187.
      Strain: 129.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-333; SER-339 AND SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNFIC_MOUSE
    AccessioniPrimary (citable) accession number: P70255
    Secondary accession number(s): O09072
    , P70256, Q3U2I9, Q99MA3, Q99MA4, Q99MA5, Q99MA6, Q99MA7, Q99MA8, Q9R1G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3