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P70255 (NFIC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor 1 C-type

Short name=NF1-C
Short name=Nuclear factor 1/C
Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/C
Short name=NF-I/C
Short name=NFI-C
TGGCA-binding protein
Gene names
Name:Nfic
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subunit structure

Binds DNA as a homodimer.

Subcellular location

Nucleus.

Tissue specificity

Highest levels in skeletal muscle. Lower levels in heart, liver, kidney, lung and brain. Very low levels in testis and spleen.

Sequence similarities

Belongs to the CTF/NF-I family.

Contains 1 CTF/NF-I DNA-binding domain.

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70255-1)

Also known as: C1A; C2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70255-2)

Also known as: C1B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MYSSPLCLTQ → M
Isoform 3 (identifier: P70255-3)

Also known as: C5;

The sequence of this isoform differs from the canonical sequence as follows:
     424-439: PALRPTRPLQTVPLWD → SWYLG
Isoform 4 (identifier: P70255-4)

Also known as: C8;

The sequence of this isoform differs from the canonical sequence as follows:
     320-361: Missing.
Isoform 5 (identifier: P70255-5)

Also known as: C9;

The sequence of this isoform differs from the canonical sequence as follows:
     362-423: Missing.
Isoform 6 (identifier: P70255-6)

Also known as: C10;

The sequence of this isoform differs from the canonical sequence as follows:
     247-374: Missing.
Isoform 7 (identifier: P70255-7)

Also known as: C11;

The sequence of this isoform differs from the canonical sequence as follows:
     247-374: Missing.
     424-439: PALRPTRPLQTVPLWD → SWYLG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Nuclear factor 1 C-type
PRO_0000100200

Regions

DNA binding1 – 195195CTF/NF-I

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1941Phosphoserine By similarity
Modified residue2941Phosphoserine By similarity
Modified residue3231Phosphoserine Ref.6
Modified residue3331Phosphoserine Ref.6
Modified residue3371Phosphoserine By similarity
Modified residue3391Phosphoserine Ref.6 Ref.7
Modified residue3431Phosphoserine Ref.6

Natural variations

Alternative sequence1 – 1010MYSSPLCLTQ → M in isoform 2.
VSP_003557
Alternative sequence247 – 374128Missing in isoform 6 and isoform 7.
VSP_007556
Alternative sequence320 – 36142Missing in isoform 4.
VSP_007557
Alternative sequence362 – 42362Missing in isoform 5.
VSP_007558
Alternative sequence424 – 43916PALRP…VPLWD → SWYLG in isoform 3 and isoform 7.
VSP_007559

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (C1A) (C2) [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: F794131A7A1B686A

FASTA43948,768
        10         20         30         40         50         60 
MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 

        70         80         90        100        110        120 
LLGEKAEVKQ KWASRLLAKL RKDIRPECRE DFVLAVTGKK APGCVLSNPD QKGKMRRIDC 

       130        140        150        160        170        180 
LRQADKVWRL DLVMVILFKG IPLESTDGER LVKAAACAHP VLCVQPHHIG VAVKELDLYL 

       190        200        210        220        230        240 
AYFVRERDAE QSSSPRTGVG SDQEDSKPIT LDTTDFQESF VTSGVFSVTE LIQVSRTPVV 

       250        260        270        280        290        300 
TGTGPNFSLG ELQGHLAYDL NPASAGMRRT LPSTSSSGSK RHKSGSMEED VDTSPGGDYY 

       310        320        330        340        350        360 
TSPNSPTSSS RNWTEDIEGG ISSPVKKTEM DKSPFNSPSP QDSPRLSSFT QHHRPVIAVH 

       370        380        390        400        410        420 
SGIARSPHPT SALHFPATPI LPQTASTYFP HTAIRYPPHL NPQDPLKDLV SLACDPATQQ 

       430 
PGPPALRPTR PLQTVPLWD 

« Hide

Isoform 2 (C1B) [UniParc].

Checksum: 348C76F604357548
Show »

FASTA43047,775
Isoform 3 (C5) [UniParc].

Checksum: 6B9FD2D21C7AC136
Show »

FASTA42847,533
Isoform 4 (C8) [UniParc].

Checksum: E66D569D32A5AD99
Show »

FASTA39744,171
Isoform 5 (C9) [UniParc].

Checksum: D6812CA58974C641
Show »

FASTA37742,137
Isoform 6 (C10) [UniParc].

Checksum: 546844CFEB48DABF
Show »

FASTA31135,067
Isoform 7 (C11) [UniParc].

Checksum: 86D75E67C14D2ECF
Show »

FASTA30033,832

References

« Hide 'large scale' references
[1]"Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: NIH Swiss.
[2]"Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Liver and Skeletal muscle.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Eye.
[4]Kane R., Martin F.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 179-439 (ISOFORMS 1; 3; 4; 5; 6 AND 7).
Strain: CD-1.
Tissue: Mammary gland.
[5]"Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 11-187.
Strain: 129.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-333; SER-339 AND SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y07692 mRNA. Translation: CAA68956.1.
Y07693 mRNA. Translation: CAA68957.1.
U57635 mRNA. Translation: AAB49930.1.
AK087539 mRNA. Translation: BAC39918.1.
AK155258 mRNA. Translation: BAE33151.1.
AF358455 mRNA. Translation: AAK21331.1.
AF358456 mRNA. Translation: AAK21332.1.
AF358457 mRNA. Translation: AAK21333.1.
AF358458 mRNA. Translation: AAK21334.1.
AF358459 mRNA. Translation: AAK21335.1.
AF358460 mRNA. Translation: AAK21336.1.
AF111265 Genomic DNA. Translation: AAD39100.1.
CCDSCCDS24058.1. [P70255-1]
CCDS35999.1. [P70255-3]
RefSeqNP_032714.1. NM_008688.3. [P70255-1]
XP_006513398.1. XM_006513335.1. [P70255-2]
UniGeneMm.426936.
Mm.453078.

3D structure databases

ProteinModelPortalP70255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201748. 1 interaction.
IntActP70255. 1 interaction.
MINTMINT-4103664.

PTM databases

PhosphoSiteP70255.

Proteomic databases

PaxDbP70255.
PRIDEP70255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020461; ENSMUSP00000020461; ENSMUSG00000055053. [P70255-1]
ENSMUST00000078185; ENSMUSP00000077317; ENSMUSG00000055053. [P70255-4]
ENSMUST00000105321; ENSMUSP00000100958; ENSMUSG00000055053. [P70255-3]
ENSMUST00000117966; ENSMUSP00000113046; ENSMUSG00000055053. [P70255-2]
GeneID18029.
KEGGmmu:18029.
UCSCuc007ghx.1. mouse. [P70255-4]
uc007ghy.1. mouse. [P70255-1]
uc007gia.1. mouse. [P70255-2]

Organism-specific databases

CTD4782.
MGIMGI:109591. Nfic.

Phylogenomic databases

eggNOGNOG252172.
GeneTreeENSGT00390000009905.
HOVERGENHBG006561.
InParanoidP70255.
KOK09170.
OMAMEGGISP.
OrthoDBEOG7WDN2K.
PhylomeDBP70255.
TreeFamTF313889.

Gene expression databases

ArrayExpressP70255.
BgeeP70255.
CleanExMM_NFIC.
GenevestigatorP70255.

Family and domain databases

InterProIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERPTHR11492. PTHR11492. 1 hit.
PfamPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTSM00523. DWA. 1 hit.
[Graphical view]
PROSITEPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFIC. mouse.
NextBio293103.
PROP70255.
SOURCESearch...

Entry information

Entry nameNFIC_MOUSE
AccessionPrimary (citable) accession number: P70255
Secondary accession number(s): O09072 expand/collapse secondary AC list , P70256, Q3U2I9, Q99MA3, Q99MA4, Q99MA5, Q99MA6, Q99MA7, Q99MA8, Q9R1G3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot