Reviewed, UniProtKB/Swiss-Prot P70236 (MP2K6_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 6
      Short name=MAP kinase kinase 6
      Short name=MAPKK 6
    EC=2.7.12.2
Alternative name(s):
    MAPK/ERK kinase 6
    SAPKK3

Gene names

Name:	Map2k6
Synonyms:	Prkmk6, Sapkk3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Probably activated by dual phosphorylation on Ser-207 and Thr-211 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	activation of MAPK activity Inferred from direct assay. Source: MGI cardiac muscle contraction Inferred from mutant phenotype. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: MGI protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Dual specificity mitogen-activated protein kinase kinase 6		PRO_0000086387
Regions
	Domain	53 – 314	262	Protein kinase
	Nucleotide binding	59 – 67	9	ATP By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	82	1	ATP By similarity
Amino acid modifications
	Modified residue	28	1	Phosphothreonine By similarity
	Modified residue	82	1	N6-acetyllysine By similarity
	Modified residue	207	1	Phosphoserine Probable
	Modified residue	211	1	Phosphothreonine Probable

Sequences

	Sequence		Length	Mass (Da)
	P70236-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 62CEF6C28AF50BAC	FASTA	334	37,432
	10 20 30 40 50 60 MSQSKGKKRN PGLKIPKEAF EQPQTSSTPP RDLDSKACIS IGNQNFEVKA DDLEPIVELG 70 80 90 100 110 120 RGAYGVVEKM RHVPSGQIMA VKRIRATVNS QEQKRLLMDL DVSMRTVDCP FTVTFYGALF 130 140 150 160 170 180 REGDVWICME LMDTSLDKFY KQVIDKGQTI PEDILGKIAV SIVKALEHLH SKLSVIHRDV 190 200 210 220 230 240 KPSNVLINTL GQVKMCDFGI SGYLVDSVAK TIDAGCKPYM APERINPELN QKGYSVKSDI 250 260 270 280 290 300 WSLGITMIEL AILRFPYDSW GTPFQQLKQV VEEPSPQLPA DKFSADFVDF TSQCLKKNSK 310 320 330 ERPTYPELMQ HPFFTVHESK AADVASFVKL ILGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and cDNA cloning of SAPKK3, the major activator of RK/p38 in stress- and cytokine-stimulated monocytes and epithelial cells." Cuenda A., Alonso G., Morrice N., Jones M., Meier R., Cohen P., Nebreda A.R. EMBO J. 15:4156-4164(1996) [PubMed: 8861944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97052 mRNA. Translation: CAA65764.1. BC075652 mRNA. Translation: AAH75652.1.
IPI	IPI00137439.
PIR	S71632.
RefSeq	NP_036073.1.
UniGene	Mm.14487
3D structure databases
HSSP	HSSP built from PDB template 1F3M based on UniProtKB Q13153.
ModBase	Search...
Protein-protein interaction databases
STRING	P70236.
PTM databases
PhosphoSite	P70236.
Proteomic databases
PRIDE	P70236.
Genome annotation databases
Ensembl	ENSMUST00000020949; ENSMUSP00000020949; ENSMUSG00000020623; Mus musculus. [Genome view]
GeneID	26399.
KEGG	mmu:26399.
UCSC	uc007mdr.1. mouse.
Organism-specific databases
CTD	26399.
MGI	MGI:1346870. Map2k6.
Phylogenomic databases
HOGENOM	P70236.
HOVERGEN	P70236.
OMA	QTSSTXR.
Enzyme and pathway databases
BRENDA	2.7.12.2. 244.
Gene expression databases
ArrayExpress	P70236.
Bgee	P70236.
CleanEx	MM_MAP2K6.
Genevestigator	P70236.
GermOnline	ENSMUSG00000020623. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304355.
SOURCE	Search...

Entry information

Entry name

MP2K6_MOUSE

Accession

Primary (citable) accession number: P70236

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)