P70211 (DCC_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 112.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Netrin receptor DCC Alternative name(s): Tumor suppressor protein DCC | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 1447 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. |
| Subunit structure | Interacts with the cytoplasmic part of UNC5A, UNC5B, UNC5C and probably UNC5D By similarity. Interacts with DSCAM. Interacts with PTK2/FAK1. Ref.3 Ref.4 |
| Subcellular location | |
| Tissue specificity | In the embryo, expressed at high levels in the developing brain and neural tube. In adult, highly expressed in brain with very low levels found in testis, heart and thymus. Isoform C is expressed only in the embryo. |
| Developmental stage | Low levels in early gestation. Highest levels expressed during mid gestation. Levels decrease in late gestation and remain at this level in the adult. |
| Post-translational modification | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity. |
| Sequence similarities | Belongs to the immunoglobulin superfamily. DCC family. Contains 6 fibronectin type-III domains. Contains 4 Ig-like C2-type (immunoglobulin-like) domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Apoptosis |
| Cellular component | Membrane |
| Coding sequence diversity | Alternative initiation Alternative splicing |
| Disease | Tumor suppressor |
| Domain | Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix |
| Molecular function | Developmental protein Receptor |
| PTM | Disulfide bond Glycoprotein Phosphoprotein Ubl conjugation |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal developmentInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membraneTraceable author statement. Source: Reactome |
| Molecular function | protein binding Inferred from physical interaction Ref.4. Source: IntAct receptor activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Dscam | Q9ERC8 | 4 | EBI-1798863,EBI-1798601 | |
| MAZ | P56270 | 2 | EBI-1798863,EBI-1809742 | From a different organism. |
| Maz | P56671 | 3 | EBI-1798863,EBI-1809712 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select] | ||||||
| Isoform A (identifier: P70211-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform C (identifier: P70211-2) The sequence of this isoform differs from the canonical sequence as follows: 819-838: Missing. | ||||||
| Isoform B (identifier: P70211-3) The sequence of this isoform differs from the canonical sequence as follows: 1-84: Missing. | ||||||
| Note: Produced by alternative initiation at Met-85 of isoform A. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 25 | 25 | Potential | ||||||||
| Chain | 26 – 1447 | 1422 | Netrin receptor DCC | PRO_0000014745 | |||||||
Regions | |||||||||||
| Topological domain | 26 – 1097 | 1072 | Extracellular Potential | ||||||||
| Transmembrane | 1098 – 1122 | 25 | Helical; Potential | ||||||||
| Topological domain | 1123 – 1447 | 325 | Cytoplasmic Potential | ||||||||
| Domain | 36 – 135 | 100 | Ig-like C2-type 1 | ||||||||
| Domain | 139 – 229 | 91 | Ig-like C2-type 2 | ||||||||
| Domain | 234 – 326 | 93 | Ig-like C2-type 3 | ||||||||
| Domain | 331 – 416 | 86 | Ig-like C2-type 4 | ||||||||
| Domain | 429 – 520 | 92 | Fibronectin type-III 1 | ||||||||
| Domain | 528 – 616 | 89 | Fibronectin type-III 2 | ||||||||
| Domain | 622 – 714 | 93 | Fibronectin type-III 3 | ||||||||
| Domain | 726 – 814 | 89 | Fibronectin type-III 4 | ||||||||
| Domain | 843 – 939 | 97 | Fibronectin type-III 5 | ||||||||
| Domain | 944 – 1041 | 98 | Fibronectin type-III 6 | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 868 | 1 | Phosphoserine By similarity | ||||||||
| Modified residue | 1073 | 1 | Phosphoserine By similarity | ||||||||
| Modified residue | 1074 | 1 | Phosphothreonine By similarity | ||||||||
| Glycosylation | 60 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 94 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 299 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 318 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 478 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 628 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 702 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 61 ↔ 117 | By similarity | |||||||||
| Disulfide bond | 161 ↔ 212 | By similarity | |||||||||
| Disulfide bond | 261 ↔ 310 | By similarity | |||||||||
| Disulfide bond | 352 ↔ 400 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 1 – 84 | 84 | Missing in isoform B. | VSP_018807 | |||||||
| Alternative sequence | 819 – 838 | 20 | Missing in isoform C. | VSP_002501 | |||||||
Sequences
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References
| [1] | "Cloning of the mouse homologue of the deleted in colorectal cancer gene (mDCC) and its expression in the developing mouse embryo." Cooper H.M., Armes P., Britto J., Gad J., Wilks A.F. Oncogene 11:2243-2254(1995) [PubMed: 8570174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C). Strain: BALB/c. Tissue: Brain. |
| [2] | Cooper H.M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [3] | "Activation of FAK and Src are receptor-proximal events required for netrin signaling." Li W., Lee J., Vikis H.G., Lee S.H., Liu G., Aurandt J., Shen T.L., Fearon E.R., Guan J.L., Han M., Rao Y., Hong K., Guan K.L. Nat. Neurosci. 7:1213-1221(2004) [PubMed: 15494734] [Abstract] Cited for: INTERACTION WITH PTK2/FAK1. |
| [4] | "DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1." Ly A., Nikolaev A., Suresh G., Zheng Y., Tessier-Lavigne M., Stein E. Cell 133:1241-1254(2008) [PubMed: 18585357] [Abstract] Cited for: INTERACTION WITH DSCAM. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | X85788 mRNA. Translation: CAA59786.1. | ||||||||||||
| IPI | IPI00137347. IPI00225034. IPI00759859. | ||||||||||||
| UniGene | Mm.167882. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| ProteinModelPortal | P70211. | ||||||||||||
| SMR | P70211. Positions 38-1047, 1410-1445. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P70211. 10 interactions. | ||||||||||||
| STRING | P70211. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P70211. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P70211. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| UCSC | uc008fop.1. mouse. uc008foq.1. mouse. | ||||||||||||
Organism-specific databases | |||||||||||||
| MGI | MGI:94869. Dcc. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | roNOG04119. | ||||||||||||
| GeneTree | ENSGT00590000082947. | ||||||||||||
| HOGENOM | HBG358293. | ||||||||||||
| HOVERGEN | HBG005455. | ||||||||||||
| InParanoid | P70211. | ||||||||||||
| OrthoDB | EOG4WM4SV. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| Reactome | REACT_112933. Cell-Cell communication. REACT_115492. Developmental Biology. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P70211. | ||||||||||||
| Bgee | P70211. | ||||||||||||
| CleanEx | MM_DCC. | ||||||||||||
| Genevestigator | P70211. | ||||||||||||
| GermOnline | ENSMUSG00000060534. Mus musculus. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR003961. Fibronectin_type3. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR010560. Neogenin_C. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.60.40.10. Ig-like_fold. 10 hits. | ||||||||||||
| Pfam | PF00041. fn3. 6 hits. PF07679. I-set. 3 hits. PF06583. Neogenin_C. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00060. FN3. 6 hits. SM00409. IG. 1 hit. SM00408. IGc2. 3 hits. [Graphical view] | ||||||||||||
| SUPFAM | SSF49265. FN_III-like. 6 hits. | ||||||||||||
| PROSITE | PS50853. FN3. 6 hits. PS50835. IG_LIKE. 4 hits. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | DCC_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P70211 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with