Reviewed, UniProtKB/Swiss-Prot P70211 (DCC_MOUSE)

Names and origin

Protein names

Recommended name:
    Netrin receptor DCC
Alternative name(s):
    Tumor suppressor protein DCC

Gene names

Name:

Dcc

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
Subunit structure	Interacts with the cytoplasmic part of UNC5A, UNC5B, UNC5C and probably UNC5D By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	In the embryo, expressed at high levels in the developing brain and neural tube. In adult, highly expressed in brain with very low levels found in testis, heart and thymus. Isoform C is expressed only in the embryo.
Developmental stage	Low levels in early gestation. Highest levels expressed during mid gestation. Levels decrease in late gestation and remain at this level in the adult.
Post-translational modification	Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity.
Sequence similarities	Belongs to the immunoglobulin superfamily. DCC family. Contains 6 fibronectin type-III domains. Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Coding sequence diversity	Alternative initiation Alternative splicing
Disease	Tumor suppressor
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Molecular function	Developmental protein Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW neuron migration Inferred from mutant phenotype. Source: MGI spinal cord ventral commissure morphogenesis Inferred from mutant phenotype. Source: MGI
Cellular component	axon Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dscam	Q9ERC8	1	EBI-1798863,EBI-1798601
MAZ	P56270	1	EBI-1798863,EBI-1809742	From a different organism.
Maz	P56671	1	EBI-1798863,EBI-1809712
Ntn1	O09118	1	EBI-1798863,EBI-1798844

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform A (identifier: P70211-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: P70211-2)

The sequence of this isoform differs from the canonical sequence as follows:
     819-838: Missing.

Isoform B (identifier: P70211-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Note: Produced by alternative initiation at Met-85 of isoform A.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1447	1422	Netrin receptor DCC		PRO_0000014745
Regions
	Topological domain	26 – 1097	1072	Extracellular Potential
	Transmembrane	1098 – 1122	25	Potential
	Topological domain	1123 – 1447	325	Cytoplasmic Potential
	Domain	36 – 135	100	Ig-like C2-type 1
	Domain	139 – 229	91	Ig-like C2-type 2
	Domain	234 – 326	93	Ig-like C2-type 3
	Domain	331 – 416	86	Ig-like C2-type 4
	Domain	429 – 520	92	Fibronectin type-III 1
	Domain	528 – 616	89	Fibronectin type-III 2
	Domain	622 – 714	93	Fibronectin type-III 3
	Domain	726 – 814	89	Fibronectin type-III 4
	Domain	843 – 939	97	Fibronectin type-III 5
	Domain	944 – 1041	98	Fibronectin type-III 6
Amino acid modifications
	Modified residue	1073	1	Phosphoserine By similarity
	Modified residue	1074	1	Phosphothreonine By similarity
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	318	1	N-linked (GlcNAc...) Potential
	Glycosylation	478	1	N-linked (GlcNAc...) Potential
	Glycosylation	628	1	N-linked (GlcNAc...) Potential
	Glycosylation	702	1	N-linked (GlcNAc...) Potential
	Disulfide bond	61 ↔ 117		By similarity
	Disulfide bond	161 ↔ 212		By similarity
	Disulfide bond	261 ↔ 310		By similarity
	Disulfide bond	352 ↔ 400		By similarity
Natural variations
	Alternative sequence	1 – 84	84	Missing in isoform B.		VSP_018807
	Alternative sequence	819 – 838	20	Missing in isoform C.		VSP_002501

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 0D1F1097C22D5B9F	FASTA	1,447	158,299
	10 20 30 40 50 60 MENSLGCVWV PKLAFVLFGA SLLSAHLQVT GFQIKPFTSL HFVSEPSDAV TMRGGNVLLN 70 80 90 100 110 120 CSAESDRGVP VIKWKKDGLI LALGMDDRKQ QLPNGSLLIQ NILHSRHHKP DEGLYQCEAS 130 140 150 160 170 180 LADSGSIISR TAKVTVAGPL RFLSQTESIT AFMGDTVLLK CEVIGEPMPT IHWQKNQQDL 190 200 210 220 230 240 NPLPGDSRVV VLPSGALQIS RLQPGDSGVY RCSARNPASI RTGNEAEVRI LSDPGLHRQL 250 260 270 280 290 300 YFLQRPSNVI AIEGKDAVLE CCVSGYPPPS FTWLRGEEVI QLRSKKYSLL GGSNLLISNV 310 320 330 340 350 360 TDDDSGTYTC VVTYKNENIS ASAELTVLVP PWFLNHPSNL YAYESMDIEF ECAVSGKPVP 370 380 390 400 410 420 TVNWMKNGDV VIPSDYFQIV GGSNLRILGV VKSDEGFYQC VAENEAGNAQ SSAQLIVPKP 430 440 450 460 470 480 AIPSSSILPS APRDVLPVLV SSRFVRLSWR PPAEAKGNIQ TFTVFFSREG DNRERALNTT 490 500 510 520 530 540 QPGSLQLTVG NLKPEAMYTF RVVAYNEWGP GESSQPIKVA TQPELQVPGP VENLHAVSTS 550 560 570 580 590 600 PTSILITWEP PAYANGPVQG YRLFCTEVST GKEQNIEVDG LSYKLEGLKK FTEYTLRFLA 610 620 630 640 650 660 YNRYGPGVST DDITVVTLSD VPSAPPQNIS LEVVNSRSIK VSWLPPPSGT QNGFITGYKI 670 680 690 700 710 720 RHRKTTRRGE METLEPNNLW YLFTGLEKGS QYSFQVSAMT VNGTGPPSNW YTAETPENDL 730 740 750 760 770 780 DESQVPDQPS SLHVRPQTNC IIMSWTPPLN PNIVVRGYII GYGVGSPYAE TVRVDSKQRY 790 800 810 820 830 840 YSIERLESSS HYVISLKAFN NAGEGVPLYE SATTRSITDP TDPVDYYPLL DDFPTSGPDV 850 860 870 880 890 900 STPMLPPVGV QAVALTHEAV RVSWADNSVP KNQKTSDVRL YTVRWRTSFS ASAKYKSEDT 910 920 930 940 950 960 TSLSYTATGL KPNTMYEFSV MVTKNRRSST WSMTAHATTY EAAPTSAPKD LTVITREGKP 970 980 990 1000 1010 1020 RAVIVSWQPP LEANGKITAY ILFYTLDKNI PIDDWIMETI SGDRLTHQIM DLSLDTMYYF 1030 1040 1050 1060 1070 1080 RIQARNVKGV GPLSDPILFR TLKVEHPDKM ANDQGRHGDG GYWPVDTNLI DRSTLNEPPI 1090 1100 1110 1120 1130 1140 GQMHPPHGSV TPQKNSNLLV ITVVTVGVLT VLVVVIVAVI CTRRSSAQQR KKRATHSGSK 1150 1160 1170 1180 1190 1200 RKGSQKDLRP PDLWIHHEEM EMKNIEKPTG TDPAGRDSPI QSCQDLTPVS HSQSETQMGS 1210 1220 1230 1240 1250 1260 KSASHSGQDT EDAGSSMSTL ERSLAARRAT RAKLMIPMEA QSSNPAVVSA IPVPTLESAQ 1270 1280 1290 1300 1310 1320 YPGILPSPTC GYPHPQFTLR PVPFPTLSVD RGFGAGRTQS VSEGPTTQQQ PMLPPAQPEH 1330 1340 1350 1360 1370 1380 PSSEEAPSRT IPTACVRPTH PLRSFANPLL PPPMSAIEPK VPYTPLLSQP GPTLPKTHVK 1390 1400 1410 1420 1430 1440 TASLGLAGKA RSPLLPVSVP TAPEVSEESH KPTEDPASVY EQDDLSEQMA SLEGLMKQLN AITGSAF
	Isoform C. Checksum: 3AE86089F5ADEE46	FASTA	1,427	156,093
	10 20 30 40 50 60 MENSLGCVWV PKLAFVLFGA SLLSAHLQVT GFQIKPFTSL HFVSEPSDAV TMRGGNVLLN 70 80 90 100 110 120 CSAESDRGVP VIKWKKDGLI LALGMDDRKQ QLPNGSLLIQ NILHSRHHKP DEGLYQCEAS 130 140 150 160 170 180 LADSGSIISR TAKVTVAGPL RFLSQTESIT AFMGDTVLLK CEVIGEPMPT IHWQKNQQDL 190 200 210 220 230 240 NPLPGDSRVV VLPSGALQIS RLQPGDSGVY RCSARNPASI RTGNEAEVRI LSDPGLHRQL 250 260 270 280 290 300 YFLQRPSNVI AIEGKDAVLE CCVSGYPPPS FTWLRGEEVI QLRSKKYSLL GGSNLLISNV 310 320 330 340 350 360 TDDDSGTYTC VVTYKNENIS ASAELTVLVP PWFLNHPSNL YAYESMDIEF ECAVSGKPVP 370 380 390 400 410 420 TVNWMKNGDV VIPSDYFQIV GGSNLRILGV VKSDEGFYQC VAENEAGNAQ SSAQLIVPKP 430 440 450 460 470 480 AIPSSSILPS APRDVLPVLV SSRFVRLSWR PPAEAKGNIQ TFTVFFSREG DNRERALNTT 490 500 510 520 530 540 QPGSLQLTVG NLKPEAMYTF RVVAYNEWGP GESSQPIKVA TQPELQVPGP VENLHAVSTS 550 560 570 580 590 600 PTSILITWEP PAYANGPVQG YRLFCTEVST GKEQNIEVDG LSYKLEGLKK FTEYTLRFLA 610 620 630 640 650 660 YNRYGPGVST DDITVVTLSD VPSAPPQNIS LEVVNSRSIK VSWLPPPSGT QNGFITGYKI 670 680 690 700 710 720 RHRKTTRRGE METLEPNNLW YLFTGLEKGS QYSFQVSAMT VNGTGPPSNW YTAETPENDL 730 740 750 760 770 780 DESQVPDQPS SLHVRPQTNC IIMSWTPPLN PNIVVRGYII GYGVGSPYAE TVRVDSKQRY 790 800 810 820 830 840 YSIERLESSS HYVISLKAFN NAGEGVPLYE SATTRSITDV STPMLPPVGV QAVALTHEAV 850 860 870 880 890 900 RVSWADNSVP KNQKTSDVRL YTVRWRTSFS ASAKYKSEDT TSLSYTATGL KPNTMYEFSV 910 920 930 940 950 960 MVTKNRRSST WSMTAHATTY EAAPTSAPKD LTVITREGKP RAVIVSWQPP LEANGKITAY 970 980 990 1000 1010 1020 ILFYTLDKNI PIDDWIMETI SGDRLTHQIM DLSLDTMYYF RIQARNVKGV GPLSDPILFR 1030 1040 1050 1060 1070 1080 TLKVEHPDKM ANDQGRHGDG GYWPVDTNLI DRSTLNEPPI GQMHPPHGSV TPQKNSNLLV 1090 1100 1110 1120 1130 1140 ITVVTVGVLT VLVVVIVAVI CTRRSSAQQR KKRATHSGSK RKGSQKDLRP PDLWIHHEEM 1150 1160 1170 1180 1190 1200 EMKNIEKPTG TDPAGRDSPI QSCQDLTPVS HSQSETQMGS KSASHSGQDT EDAGSSMSTL 1210 1220 1230 1240 1250 1260 ERSLAARRAT RAKLMIPMEA QSSNPAVVSA IPVPTLESAQ YPGILPSPTC GYPHPQFTLR 1270 1280 1290 1300 1310 1320 PVPFPTLSVD RGFGAGRTQS VSEGPTTQQQ PMLPPAQPEH PSSEEAPSRT IPTACVRPTH 1330 1340 1350 1360 1370 1380 PLRSFANPLL PPPMSAIEPK VPYTPLLSQP GPTLPKTHVK TASLGLAGKA RSPLLPVSVP 1390 1400 1410 1420 TAPEVSEESH KPTEDPASVY EQDDLSEQMA SLEGLMKQLN AITGSAF
	Isoform B. Checksum: FF0ADBA076D6AC61	FASTA	1,363	149,303
	10 20 30 40 50 60 MDDRKQQLPN GSLLIQNILH SRHHKPDEGL YQCEASLADS GSIISRTAKV TVAGPLRFLS 70 80 90 100 110 120 QTESITAFMG DTVLLKCEVI GEPMPTIHWQ KNQQDLNPLP GDSRVVVLPS GALQISRLQP 130 140 150 160 170 180 GDSGVYRCSA RNPASIRTGN EAEVRILSDP GLHRQLYFLQ RPSNVIAIEG KDAVLECCVS 190 200 210 220 230 240 GYPPPSFTWL RGEEVIQLRS KKYSLLGGSN LLISNVTDDD SGTYTCVVTY KNENISASAE 250 260 270 280 290 300 LTVLVPPWFL NHPSNLYAYE SMDIEFECAV SGKPVPTVNW MKNGDVVIPS DYFQIVGGSN 310 320 330 340 350 360 LRILGVVKSD EGFYQCVAEN EAGNAQSSAQ LIVPKPAIPS SSILPSAPRD VLPVLVSSRF 370 380 390 400 410 420 VRLSWRPPAE AKGNIQTFTV FFSREGDNRE RALNTTQPGS LQLTVGNLKP EAMYTFRVVA 430 440 450 460 470 480 YNEWGPGESS QPIKVATQPE LQVPGPVENL HAVSTSPTSI LITWEPPAYA NGPVQGYRLF 490 500 510 520 530 540 CTEVSTGKEQ NIEVDGLSYK LEGLKKFTEY TLRFLAYNRY GPGVSTDDIT VVTLSDVPSA 550 560 570 580 590 600 PPQNISLEVV NSRSIKVSWL PPPSGTQNGF ITGYKIRHRK TTRRGEMETL EPNNLWYLFT 610 620 630 640 650 660 GLEKGSQYSF QVSAMTVNGT GPPSNWYTAE TPENDLDESQ VPDQPSSLHV RPQTNCIIMS 670 680 690 700 710 720 WTPPLNPNIV VRGYIIGYGV GSPYAETVRV DSKQRYYSIE RLESSSHYVI SLKAFNNAGE 730 740 750 760 770 780 GVPLYESATT RSITDPTDPV DYYPLLDDFP TSGPDVSTPM LPPVGVQAVA LTHEAVRVSW 790 800 810 820 830 840 ADNSVPKNQK TSDVRLYTVR WRTSFSASAK YKSEDTTSLS YTATGLKPNT MYEFSVMVTK 850 860 870 880 890 900 NRRSSTWSMT AHATTYEAAP TSAPKDLTVI TREGKPRAVI VSWQPPLEAN GKITAYILFY 910 920 930 940 950 960 TLDKNIPIDD WIMETISGDR LTHQIMDLSL DTMYYFRIQA RNVKGVGPLS DPILFRTLKV 970 980 990 1000 1010 1020 EHPDKMANDQ GRHGDGGYWP VDTNLIDRST LNEPPIGQMH PPHGSVTPQK NSNLLVITVV 1030 1040 1050 1060 1070 1080 TVGVLTVLVV VIVAVICTRR SSAQQRKKRA THSGSKRKGS QKDLRPPDLW IHHEEMEMKN 1090 1100 1110 1120 1130 1140 IEKPTGTDPA GRDSPIQSCQ DLTPVSHSQS ETQMGSKSAS HSGQDTEDAG SSMSTLERSL 1150 1160 1170 1180 1190 1200 AARRATRAKL MIPMEAQSSN PAVVSAIPVP TLESAQYPGI LPSPTCGYPH PQFTLRPVPF 1210 1220 1230 1240 1250 1260 PTLSVDRGFG AGRTQSVSEG PTTQQQPMLP PAQPEHPSSE EAPSRTIPTA CVRPTHPLRS 1270 1280 1290 1300 1310 1320 FANPLLPPPM SAIEPKVPYT PLLSQPGPTL PKTHVKTASL GLAGKARSPL LPVSVPTAPE 1330 1340 1350 1360 VSEESHKPTE DPASVYEQDD LSEQMASLEG LMKQLNAITG SAF

References

[1]	"Cloning of the mouse homologue of the deleted in colorectal cancer gene (mDCC) and its expression in the developing mouse embryo." Cooper H.M., Armes P., Britto J., Gad J., Wilks A.F. Oncogene 11:2243-2254(1995) [PubMed: 8570174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C). Strain: BALB/c. Tissue: Brain.
[2]	Cooper H.M. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85788 mRNA. Translation: CAA59786.1.
IPI	IPI00137347. IPI00225034. IPI00759859.
UniGene	Mm.167882
3D structure databases
HSSP	HSSP built from PDB template 1IE5 based on UniProtKB P13590.
ModBase	Search...
Protein-protein interaction databases
IntAct	P70211. 5 interactions.
STRING	P70211.
PTM databases
PhosphoSite	P70211.
Proteomic databases
PRIDE	P70211.
Genome annotation databases
Ensembl	ENSMUST00000114943; ENSMUSP00000110593; ENSMUSG00000060534; Mus musculus. [Genome view]
UCSC	uc008fop.1. mouse.
Organism-specific databases
MGI	MGI:94869. Dcc.
Phylogenomic databases
HOGENOM	P70211.
HOVERGEN	P70211.
Gene expression databases
ArrayExpress	P70211.
Bgee	P70211.
CleanEx	MM_DCC.
Genevestigator	P70211.
GermOnline	ENSMUSG00000060534. Mus musculus.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR010560. Neogenin_C. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 6 hits. G3DSA:2.60.40.10. Ig-like_fold. 4 hits.
Pfam	PF00041. fn3. 6 hits. PF07679. I-set. 4 hits. PF06583. Neogenin_C. 1 hit. [Graphical view]
PRINTS	PR00014. FNTYPEIII.
SMART	SM00060. FN3. 6 hits. SM00409. IG. 1 hit. SM00408. IGc2. 3 hits. [Graphical view]
PROSITE	PS50853. FN3. 6 hits. PS50835. IG_LIKE. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

DCC_MOUSE

Accession

Primary (citable) accession number: P70211

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	October 13, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)