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P70208 (PLXA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plexin-A3
Gene names
Name:Plxna3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1872 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Tissue specificity

Detected in embryonic hindbrain, spinal cord, dorsal root ganglion, trigeminal ganglion and superior cervical ganglion. In newborns, detected throughout all layers of the hippocampus. Ref.1 Ref.5 Ref.7

Disruption phenotype

No visible phenotype, but causes subtle changes in the central nervous system. Mice exhibit altered apical dendrite spine morphology in pyramidal neurons. Mice exhibit defasciculation of the facial branchiomotor nerve and of the ophthalmic branch of the trigeminus, with variable severity. The number of neurons in the dorsal root ganglion is higher than normal, probably due to reduced neuronal apoptosis. In mice lacking both Plxna3 and Plxna4, migrating neurons do not show the normal response to Sema3A and Sema3F and do not migrate away from these semaphorins (in vitro). Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Sequence similarities

Belongs to the plexin family.

Contains 4 IPT/TIG domains.

Contains 1 Sema domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P70208-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P70208-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1072-1148: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 18721853Plexin-A3
PRO_0000411104

Regions

Topological domain20 – 12201201Extracellular Potential
Transmembrane1221 – 124121Helical; Potential
Topological domain1242 – 1872631Cytoplasmic Potential
Domain20 – 489470Sema
Domain841 – 93494IPT/TIG 1
Domain936 – 102186IPT/TIG 2
Domain1024 – 1123100IPT/TIG 3
Domain1126 – 121287IPT/TIG 4
Coiled coil1240 – 129455 Potential

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation5491N-linked (GlcNAc...) Potential
Glycosylation11631N-linked (GlcNAc...) Potential
Disulfide bond78 ↔ 87 By similarity
Disulfide bond113 ↔ 121 By similarity
Disulfide bond267 ↔ 388 By similarity
Disulfide bond283 ↔ 339 By similarity
Disulfide bond357 ↔ 376 By similarity
Disulfide bond492 ↔ 509 By similarity
Disulfide bond498 ↔ 540 By similarity
Disulfide bond501 ↔ 518 By similarity
Disulfide bond512 ↔ 524 By similarity
Disulfide bond575 ↔ 595 By similarity

Natural variations

Alternative sequence1072 – 114877Missing in isoform 2.
VSP_041609

Experimental info

Mutagenesis12791L → R: Strongly reduced response to semaphorin. Ref.10
Mutagenesis13001Y → A: Almost abolishes response to semaphorin. Ref.10
Mutagenesis13091F → A: Almost abolishes response to semaphorin. Ref.10
Mutagenesis1407 – 14082RR → AA: Abolishes response to semaphorin.
Mutagenesis14531I → R: Abolishes response to semaphorin. Ref.10
Mutagenesis14641L → G: Almost abolishes response to semaphorin. Ref.10
Mutagenesis15061Q → A: Abolishes response to semaphorin. Ref.10
Mutagenesis1631 – 16322HL → AA: Abolishes response to semaphorin.
Sequence conflict16271T → A in BAC33681. Ref.4
Sequence conflict18611L → P in BAA13190. Ref.1

Secondary structure

.................................................................................... 1872
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 39D57886A0C4830A

FASTA1,872207,958
        10         20         30         40         50         60 
MPTVCLLPLL FFTIGGCLGS SRPFRTFVVT DTTLTHLAVH RVTGEVFVGA VNRVFKLAPN 

        70         80         90        100        110        120 
LTELRAHVTG PIEDNARCYP PPSMRVCSHR LVPVDNVNKL LLIDYAARRL VACGSIWQGI 

       130        140        150        160        170        180 
CQFLRLDDLF KLGEPHHRKE HYLSGAQEPD SMAGVIVEQV QGPSKLFVGT AVDGKSEYFP 

       190        200        210        220        230        240 
TLSSRKLIDD EDSGDMFSLV YQDEFVSSQI KIPSDTLSLY PAFDIYYIYG FVSASFVYFL 

       250        260        270        280        290        300 
TLQLDTQQTL LDTAGEKFFT SKIVRMCAGD SEFYSYVEFP IGCSWRGVEY RLVQSAHLAK 

       310        320        330        340        350        360 
PGLLLAQALG VPADEDVLFT IFSQGQKNRA NPPRQTILCL FTLSSINAHI RRRIQSCYRG 

       370        380        390        400        410        420 
EGTLALPWLL NKELPCINTP LQINGNFCGL VLNQPLGGLH VIEGLPLLAD STDGMASVAA 

       430        440        450        460        470        480 
YTYHQHSVVF IGTRSGNLKK VRVDGSQDAQ LYETVSVVQG SPILRDLLFS PDHRHIYLLS 

       490        500        510        520        530        540 
EKQVSQLPVE TCEQYLSCAA CLGSGDPHCG WCVLQHRCCR EGACPGASAP HGFAEELSKC 

       550        560        570        580        590        600 
IQVRVRPNNV SVTSSGVQLT VAMRNVPDLS VGVSCSFEEV TESEAILLPS GELRCPSPSL 

       610        620        630        640        650        660 
QELQTLTRGH GATHTVRLQL LSMETGVRFA GVDFVFYNCS ALQSCMSCVG SPYPCHWCKY 

       670        680        690        700        710        720 
RHVCTSHPHE CSFQEGRVHS PEGCPEILPQ GDLLIPVGVM QPLTLRAKNL PQPQSGQKNY 

       730        740        750        760        770        780 
ECVVRVQGRQ HRVPAVRFNS SSVQCQNASY FYEGDEFGDT ELDFSVVWDG DFPIDKPPSF 

       790        800        810        820        830        840 
RALLYKCWAQ RPSCGLCLKA DPRFNCGWCI SEHRCQLRAH CPAPKSNWMH PSQKGARCSH 

       850        860        870        880        890        900 
PRITQIHPLT GPKEGGTRVT IVGENLGLTS REVGLRVAGV RCNSIPTEYV SAERIVCEME 

       910        920        930        940        950        960 
ESLVPSPPPG PAELCVGDCS ADFRTQSQQL YSFVTPTFDR VSPSRGPASG GTRLTISGIS 

       970        980        990       1000       1010       1020 
LDAGSRVTVI IRDGECQFVR RDAEAIVCIS PVSTLGPSQS PITLAIDHAN ISNTGVIYTY 

      1030       1040       1050       1060       1070       1080 
TQDPTVTHLE PTWSIINGST SITVSGTHLL TVQEPRVRAK YRGIETTNTC QVINDTAMLC 

      1090       1100       1110       1120       1130       1140 
KAPGIFLGHP QPRAQGEHPD EFGFLLDHVQ AARSLNRSSF TYYPDPSFEP LGPSGVLDVK 

      1150       1160       1170       1180       1190       1200 
PGSHVVLKGK NLIPAAAGSS RLNYTVLIGG QPCALTVSDT QLLCDSPSQT GRQPVMVLVG 

      1210       1220       1230       1240       1250       1260 
GLEFWLGTLH ITADRALTLP AMVGLAAGGG LLLLAITVVL VAYKRKTQDA DRTLKRLQLQ 

      1270       1280       1290       1300       1310       1320 
MDNLESRVAL ECKEAFAELQ TDINELTNHM DGVQIPFLDY RTYAVRVLFP GIEAHPVLKE 

      1330       1340       1350       1360       1370       1380 
LDTPPNVEKA LRLFGQLLHS RAFLLTFIHT LEAQSSFSMR DRGTVASLTM VALQSRLDYA 

      1390       1400       1410       1420       1430       1440 
TGLLKQLLAD LIEKNLESKN HPKLLLRRTE SVAEKMLTNW FTFLLHKFLK ECAGEPLFLL 

      1450       1460       1470       1480       1490       1500 
YCAIKQQMEK GPIDAITGEA RYSLSEDKLI RQQIDYKTLT LHCVCPESEG SAQVPVKVLN 

      1510       1520       1530       1540       1550       1560 
CDSITQAKDK LLDTVYKGIP YSQRPKAEDM DLEWRQGRMA RIILQDEDIT TKIECDWKRV 

      1570       1580       1590       1600       1610       1620 
NSLAHYQVTD GSLVALVPKQ VSAYNMANSF TFTRSLSRYE SLLRAASSPD SLRSRAPMLT 

      1630       1640       1650       1660       1670       1680 
PDQEAGTKLW HLVRNHDHTD HREGDRGSKM VSEIYLTRLL ATKGTLQKFV DDLFETVFST 

      1690       1700       1710       1720       1730       1740 
AHRGSALPLA IKYMFDFLDE QADQRQISDP DVRHTWKSNC LPLRFWVNVI KNPQFVFDIH 

      1750       1760       1770       1780       1790       1800 
KNSITDACLS VVAQTFMDSC STSEHRLGKD SPSNKLLYAK DIPNYKSWVE RYYRDIAKMA 

      1810       1820       1830       1840       1850       1860 
SISDQDMDAY LVEQSRLHAN DFNVLSALSE LYFYVTKYRQ EILTSLDRDA SCRKHKLRQK 

      1870 
LEQIITLVSS SS 

« Hide

Isoform 2 [UniParc].

Checksum: 2E590AF8E22FEBA5
Show »

FASTA1,795199,604

References

« Hide 'large scale' references
[1]"Identification of plexin family molecules in mice."
Kameyama T., Murakami Y., Suto F., Kawakami A., Takagi S., Hirata T., Fujisawa H.
Biochem. Biophys. Res. Commun. 226:396-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Embryonic brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[3]"Towards functional annotation of all Xq28 genes: expression and intracellular localization analyses reveal novel candidates for disease genes."
Kolb-Kokocinski A.A., Mehrle A., Bechtel S., Wellenreuther R., Simpson J., Pepperkok R., Wiemann S., Poustka A.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1116-1560.
Strain: NMRI.
Tissue: Embryo.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1138-1872.
Strain: C57BL/6J.
[5]"Plexin-A3 mediates semaphorin signaling and regulates the development of hippocampal axonal projections."
Cheng H.J., Bagri A., Yaron A., Stein E., Pleasure S.J., Tessier-Lavigne M.
Neuron 32:249-263(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[6]"Plexin-A3 and plexin-A4 restrict the migration of sympathetic neurons but not their neural crest precursors."
Waimey K.E., Huang P.H., Chen M., Cheng H.J.
Dev. Biol. 315:448-458(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[7]"Plexin A3 and plexin A4 convey semaphorin signals during facial nerve development."
Schwarz Q., Waimey K.E., Golding M., Takamatsu H., Kumanogoh A., Fujisawa H., Cheng H.J., Ruhrberg C.
Dev. Biol. 324:1-9(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
[8]"The Semaphorin receptor PlexinA3 mediates neuronal apoptosis during dorsal root ganglia development."
Ben-Zvi A., Manor O., Schachner M., Yaron A., Tessier-Lavigne M., Behar O.
J. Neurosci. 28:12427-12432(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[9]"Secreted semaphorins control spine distribution and morphogenesis in the postnatal CNS."
Tran T.S., Rubio M.E., Clem R.L., Johnson D., Case L., Tessier-Lavigne M., Huganir R.L., Ginty D.D., Kolodkin A.L.
Nature 462:1065-1069(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[10]"Crystal structure of the plexin A3 intracellular region reveals an autoinhibited conformation through active site sequestration."
He H., Yang T., Terman J.R., Zhang X.
Proc. Natl. Acad. Sci. U.S.A. 106:15610-15615(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1170-1795, FUNCTION, MUTAGENESIS OF LEU-1279; TYR-1300; PHE-1309; 1407-ARG-ARG-1408; ILE-1453; LEU-1464; GLN-1506 AND 1631-HIS-HIS-1632.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86950 mRNA. Translation: BAA13190.1.
BC093482 mRNA. Translation: AAH93482.1.
AJ748653 mRNA. Translation: CAG38687.1.
AK049319 mRNA. Translation: BAC33681.1.
PIRJC4976.
RefSeqNP_032909.2. NM_008883.2.
XP_006527963.1. XM_006527900.1.
UniGeneMm.1685.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IG3X-ray1.99A1247-1872[»]
ProteinModelPortalP70208.
SMRP70208. Positions 1247-1871.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48959N.
STRING10090.ENSMUSP00000004326.

Proteomic databases

PRIDEP70208.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004326; ENSMUSP00000004326; ENSMUSG00000031398. [P70208-1]
GeneID18846.
KEGGmmu:18846.
UCSCuc009too.1. mouse. [P70208-1]
uc012hku.1. mouse. [P70208-2]

Organism-specific databases

CTD55558.
MGIMGI:107683. Plxna3.

Phylogenomic databases

GeneTreeENSGT00750000117292.
HOGENOMHOG000231377.
HOVERGENHBG105711.
InParanoidP70208.
KOK06820.
OMASHPHECS.
OrthoDBEOG7Q8CM9.
TreeFamTF312962.

Gene expression databases

BgeeP70208.
GenevestigatorP70208.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.60.40.10. 4 hits.
InterProIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR016201. Plexin-like_fold.
IPR013548. Plexin_cytoplasmic_RasGAP_dom.
IPR002165. Plexin_repeat.
IPR008936. Rho_GTPase_activation_prot.
IPR001627. Semap_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamPF08337. Plexin_cytopl. 1 hit.
PF01437. PSI. 3 hits.
PF01403. Sema. 1 hit.
PF01833. TIG. 4 hits.
[Graphical view]
SMARTSM00429. IPT. 4 hits.
SM00423. PSI. 3 hits.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF81296. SSF81296. 4 hits.
PROSITEPS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP70208.
NextBio295224.
PROP70208.
SOURCESearch...

Entry information

Entry namePLXA3_MOUSE
AccessionPrimary (citable) accession number: P70208
Secondary accession number(s): A5D6Q5, Q684J0, Q8BWZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot