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P70207 (PLXA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plexin-A2

Short name=Plex 2
Short name=Plexin-2
Gene names
Name:Plxna2
Synonyms:Kiaa0463
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1894 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Ref.4 Ref.5 Ref.6

Subunit structure

Homodimer. Interacts with RND1 By similarity. Interacts directly with NRP1 and NRP2. The PLXNA2 homodimer interacts with a SEMA6A homodimer, giving rise to a heterotetramer. Ref.4 Ref.5 Ref.6 Ref.7

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.6 Ref.7.

Sequence similarities

Belongs to the plexin family.

Contains 4 IPT/TIG domains.

Contains 1 Sema domain.

Sequence caution

The sequence AAH68155.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAA13189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Potential
Chain35 – 18941860Plexin-A2
PRO_0000232748

Regions

Topological domain35 – 12371203Extracellular Potential
Transmembrane1238 – 125821Helical; Potential
Topological domain1259 – 1894636Cytoplasmic Potential
Domain35 – 508474Sema
Domain858 – 95194IPT/TIG 1
Domain954 – 103784IPT/TIG 2
Domain1041 – 113999IPT/TIG 3
Domain1143 – 122886IPT/TIG 4
Coiled coil1261 – 131050 Potential

Amino acid modifications

Glycosylation151N-linked (GlcNAc...) Potential
Glycosylation761N-linked (GlcNAc...) Ref.6 Ref.7
Glycosylation1631N-linked (GlcNAc...) Ref.6
Glycosylation3271N-linked (GlcNAc...) Ref.6
Glycosylation5981N-linked (GlcNAc...) Ref.6
Glycosylation6961N-linked (GlcNAc...) Potential
Glycosylation7561N-linked (GlcNAc...) Potential
Glycosylation11801N-linked (GlcNAc...) Potential
Glycosylation12051N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 103 Ref.6 Ref.7
Disulfide bond129 ↔ 137 Ref.6 Ref.7
Disulfide bond284 ↔ 405 Ref.6 Ref.7
Disulfide bond300 ↔ 356 Ref.6 Ref.7
Disulfide bond374 ↔ 393 Ref.6 Ref.7
Disulfide bond511 ↔ 528 Ref.6 Ref.7
Disulfide bond517 ↔ 559 Ref.6 Ref.7
Disulfide bond520 ↔ 537 Ref.6 Ref.7
Disulfide bond531 ↔ 543 Ref.6 Ref.7
Disulfide bond594 ↔ 613 Ref.6 Ref.7

Experimental info

Mutagenesis1931D → K: Abolishes interaction with SEMA6A. Ref.7
Mutagenesis2211F → A or R: Abolishes interaction with SEMA6A. Ref.6 Ref.7
Mutagenesis3961A → E: Abolishes interaction with SEMA6A. Ref.6 Ref.7
Sequence conflict2111A → P in AAH68155. Ref.2

Secondary structure

........................................................................................................................................ 1894
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P70207 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 9F6C34F48BC29CEE

FASTA1,894211,535
        10         20         30         40         50         60 
MEQRRFYLRA MQADNLSVVL LSVAWLLLAR GTTGMPQYST FHSENRDWTF NHLTVHRRTG 

        70         80         90        100        110        120 
AVYVGAINRV YKLTGNLTIQ VAHKTGPEED NKACYPPLIV QPCSEVLTLT NNVNKLLIID 

       130        140        150        160        170        180 
YSENRLLACG SLYQGVCKLL RLDDLFILVE PSHKKEHYLS SVNKTGTMYG VIVRSEGEDG 

       190        200        210        220        230        240 
KLFIGTAVDG KQDYFPTLSS RKLPRDPESS AMLDYELHSD FVSSLIKIPS DTLALVSHFD 

       250        260        270        280        290        300 
IFYIYGFASG GFVYFLTVQP ETPDGMAINS AGDLFYTSRI VRLCKDDPKF HSYVSLPFGC 

       310        320        330        340        350        360 
TRAGVEYRLL QAAYLAKPGE ALAQAFNISS DEDVLFAIFS KGQKQYHHPP DDSALCAFPI 

       370        380        390        400        410        420 
RAINLQIKER LQSCYHGEGN LELNWLLGKD VQCTKAPVPI DDNFCGLDIN QPLGGSTPVE 

       430        440        450        460        470        480 
GLTLYTTSRD RLTSVASYVY NGYSVVFVGT KSGKLKKIRA DGPPHGGVQY EMVSVFKDGS 

       490        500        510        520        530        540 
PILRDMAFSI NQLYLYVMSE RQVTRVPVES CEQYTTCGEC LSSGDPHCGW CALHNMCSRR 

       550        560        570        580        590        600 
DKCQRAWEAN RFAASISQCM SLEVHPNSIS VSDHSRLLSL VVNDAPNLSE GIACAFGNLT 

       610        620        630        640        650        660 
EVEGQVSGSQ VICISPGPKD VPVIPLDQDW FGLELQLRSK ETGKIFVSTE FKFYNCSAHQ 

       670        680        690        700        710        720 
LCLSCVNSAF RCHWCKYRNL CTHDPTTCSF QEGRINVSED CPQLVPTEEI LIPVGEVKPI 

       730        740        750        760        770        780 
TLKARNLPQP QSGQRGYECV LSIQGAVHRV PALRFNSSSV QCQNSSYQYD GMDISNLAVD 

       790        800        810        820        830        840 
FAVVWNGNFI IDNPQDLKVH LYKCAAQRES CGLCLKADHK FECGWCSGER RCTLHQHCPS 

       850        860        870        880        890        900 
TSSPWLDWSS HNVKCSNPQI TEILTVSGPP EGGTRVTIHG VNLGLDFSEI AHHVQVAGVP 

       910        920        930        940        950        960 
CTPIPGEYII AEQIVCEMGH AVIGTTSGPV RLCIGECKPE FMTKSHQQYT FVNPSVLSLS 

       970        980        990       1000       1010       1020 
PIRGPESGGT MVTITGHYLG AGSSVAVYLG NQTCEFYGRS MNEIVCVSPP SSNGLGPVPV 

      1030       1040       1050       1060       1070       1080 
SVSVDRARVD SSLQFEYIDD PRVQRIEPEW SITSGHTPLT ITGFNLDVIQ EPRVRVKFNG 

      1090       1100       1110       1120       1130       1140 
KESVNVCTVV NTTTLTCLAP SLTSDYRPGL DTVERPDEFG FLFNNVQSLL IYNDTKFIYY 

      1150       1160       1170       1180       1190       1200 
PNPTFELLSP TGILDQKPGS PIILKGKNLC PPASGGAKLN YTVMIGETPC TVTVSETQLL 

      1210       1220       1230       1240       1250       1260 
CEPPNLTGQH KVMVHVGGMV FSPGSVSVIS DSLLTLPAII SIAAGGSLLL IIVIIVLIAY 

      1270       1280       1290       1300       1310       1320 
KRKSRENDLT LKRLQMQMDN LESRVALECK EAFAELQTDI NELTSDLDRS GIPYLDYRTY 

      1330       1340       1350       1360       1370       1380 
AMRVLFPGIE DHPVLRELEV QGNGQQHVEK ALKLFAQLIN NKVFLLTFIR TLELQRSFSM 

      1390       1400       1410       1420       1430       1440 
RDRGNVASLI MTGLQGRLEY ATDVLKQLLS DLIDKNLENK NHPKLLLRRT ESVAEKMLTN 

      1450       1460       1470       1480       1490       1500 
WFAFLLHKFL KECAGEPLFM LYCAIKQQME KGPIDAITGE ARYSLSEDKL IRQQIEYKTL 

      1510       1520       1530       1540       1550       1560 
ILNCVNPDNE NSPEIPVKVL NCDTITQVKE KILDAVYKNV PYSQRPRAVD MDLEWRQGRI 

      1570       1580       1590       1600       1610       1620 
ARVVLQDEDI TTKIEGDWKR LNTLMHYQVS DRSVVALVPK QTSSYNIPAS ASISRTSISR 

      1630       1640       1650       1660       1670       1680 
YDSSFRYTGS PDSLRSRVPM ITPDLESGVK VWHLVKNHDH GDQKEGDRGS KMVSEIYLTR 

      1690       1700       1710       1720       1730       1740 
LLATKGTLQK FVDDLFETLF STVHRGSALP LAIKYMFDFL DEQADRHSIH DTDVRHTWKS 

      1750       1760       1770       1780       1790       1800 
NCLPLRFWVN VIKNPQFVFD IHKGSITDAC LSVVAQTFMD SCSTSEHRLG KDSPSNKLLY 

      1810       1820       1830       1840       1850       1860 
AKDIPSYKNW VERYYADIAK LPAISDQDMN AYLAEQSRLH ATEFNMLSAL NEIYSYVSKY 

      1870       1880       1890 
SEELIGALEQ DEQARRQRLA YKVEHLINAM SIES 

« Hide

References

« Hide 'large scale' references
[1]"Identification of plexin family molecules in mice."
Kameyama T., Murakami Y., Suto F., Kawakami A., Takagi S., Hirata T., Fujisawa H.
Biochem. Biophys. Res. Commun. 226:396-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Colon.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 366-1894.
Tissue: Brain.
[4]"Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors."
Takahashi T., Fournier A., Nakamura F., Wang L.-H., Murakami Y., Kalb R.G., Fujisawa H., Strittmatter S.M.
Cell 99:59-69(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NRP1 AND SEMA3A.
[5]"Plexin/neuropilin complexes mediate repulsion by the axonal guidance signal semaphorin 3A."
Rohm B., Ottemeyer A., Lohrum M., Pueschel A.W.
Mech. Dev. 93:95-104(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NRP1; NRP2 AND SEMA3A.
[6]"Structural basis of semaphorin-plexin signalling."
Janssen B.J., Robinson R.A., Perez-Branguli F., Bell C.H., Mitchell K.J., Siebold C., Jones E.Y.
Nature 467:1118-1122(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 35-701 IN COMPLEX WITH SEMA6A, INTERACTION WITH SEMA6A, FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-76; ASN-163; ASN-327 AND ASN-598, MUTAGENESIS OF PHE-221 AND ALA-396, DISULFIDE BONDS.
[7]"Structural basis for semaphorin signalling through the plexin receptor."
Nogi T., Yasui N., Mihara E., Matsunaga Y., Noda M., Yamashita N., Toyofuku T., Uchiyama S., Goshima Y., Kumanogoh A., Takagi J.
Nature 467:1123-1127(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 31-561 IN COMPLEX WITH SEMA6A, INTERACTION WITH SEMA6A, SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-76, MUTAGENESIS OF ASP-193; PHE-221 AND ALA-396, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86949 mRNA. Translation: BAA13189.1. Different initiation.
BC024509 mRNA. Translation: AAH24509.1.
BC056475 mRNA. Translation: AAH56475.1.
BC068155 mRNA. Translation: AAH68155.1. Different initiation.
AK122289 mRNA. Translation: BAC65571.1.
CCDSCCDS35827.1.
PIRJC4975.
RefSeqNP_032908.2. NM_008882.2.
UniGeneMm.2251.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AL8X-ray3.60B31-561[»]
3AL9X-ray2.10A/B31-561[»]
3OKTX-ray2.30A35-703[»]
3OKYX-ray2.20A35-703[»]
4GZAX-ray7.00A/B/C/D/E/F33-703[»]
ProteinModelPortalP70207.
SMRP70207. Positions 36-702, 809-1040, 1273-1890.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202262. 1 interaction.
DIPDIP-32252N.
IntActP70207. 1 interaction.

PTM databases

PhosphoSiteP70207.

Proteomic databases

MaxQBP70207.
PaxDbP70207.
PRIDEP70207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027952; ENSMUSP00000027952; ENSMUSG00000026640.
GeneID18845.
KEGGmmu:18845.
UCSCuc007een.1. mouse.

Organism-specific databases

CTD5362.
MGIMGI:107684. Plxna2.
RougeSearch...

Phylogenomic databases

eggNOGNOG254546.
GeneTreeENSGT00750000117292.
HOGENOMHOG000231377.
HOVERGENHBG105711.
InParanoidP70207.
KOK06820.
OMACKDDPKF.
OrthoDBEOG7Q8CM9.
PhylomeDBP70207.
TreeFamTF312962.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressP70207.
BgeeP70207.
CleanExMM_PLXNA2.
GenevestigatorP70207.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.60.40.10. 4 hits.
InterProIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR016201. Plexin-like_fold.
IPR013548. Plexin_cytoplasmic_RasGAP_dom.
IPR002165. Plexin_repeat.
IPR008936. Rho_GTPase_activation_prot.
IPR001627. Semap_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamPF08337. Plexin_cytopl. 1 hit.
PF01437. PSI. 3 hits.
PF01403. Sema. 1 hit.
PF01833. TIG. 4 hits.
[Graphical view]
SMARTSM00429. IPT. 4 hits.
SM00423. PSI. 3 hits.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 2 hits.
SSF48350. SSF48350. 2 hits.
SSF81296. SSF81296. 4 hits.
PROSITEPS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLXNA2. mouse.
EvolutionaryTraceP70207.
NextBio295220.
PROP70207.
SOURCESearch...

Entry information

Entry namePLXA2_MOUSE
AccessionPrimary (citable) accession number: P70207
Secondary accession number(s): Q6NVE6 expand/collapse secondary AC list , Q6PHN4, Q80TZ7, Q8R1I4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot