Reviewed,
UniProtKB/Swiss-Prot P70206 (PLXA1_MOUSE)
Last modified
May 5, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Plexin-A1 Short name=Plexin-1 Short name=Plex 1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 1894 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Ref.3 Ref.4 |
| Subunit structure | Interacts directly with NRP1 and NRP2. Interacts with FARP2, RND1 and KDR/VEGFR2. Binding of SEMA3A leads to dissociation of FARP2. Interacts with WITH CRMP1, DPYSL2/CRMP2, DPYSL3/CRMP3 and DPYSL4/CRMP4. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 |
| Subcellular location | Membrane; Single-pass type I membrane protein Potential. |
| Tissue specificity | |
| Sequence similarities | Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Coiled coil Repeat Signal Transmembrane |
| PTM | Glycoprotein Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | multicellular organismal development Inferred from electronic annotation. Source: InterPro signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellularInferred from electronic annotation. Source: InterPro membrane fractionInferred from direct assay. Source: MGI plasma membraneInferred by curator. Source: MGI |
| Molecular function | protein binding Ref.3 Inferred from physical interaction. Source: MGI semaphorin receptor activityInferred from physical interaction. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 27 | 27 | Potential | ||||||
| Chain | 28 – 1894 | 1867 | Plexin-A1 | PRO_0000232746 | |||||
Regions | |||||||||
| Topological domain | 28 – 1242 | 1215 | Extracellular Potential | ||||||
| Transmembrane | 1243 – 1263 | 21 | Potential | ||||||
| Topological domain | 1264 – 1894 | 631 | Cytoplasmic Potential | ||||||
| Domain | 28 – 510 | 483 | Sema | ||||||
| Domain | 862 – 957 | 96 | IPT/TIG 1 | ||||||
| Domain | 959 – 1043 | 85 | IPT/TIG 2 | ||||||
| Domain | 1046 – 1145 | 100 | IPT/TIG 3 | ||||||
| Domain | 1148 – 1234 | 87 | IPT/TIG 4 | ||||||
| Coiled coil | 1262 – 1315 | 54 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 120 | 1 | Phosphotyrosine Ref.9 | ||||||
| Glycosylation | 75 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 658 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 670 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 699 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1041 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1185 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1210 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 1266 – 1268 | 3 | KRK → AAA: Loss of interaction with FARP2. Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a neuronal cell surface molecule, plexin, in mice." Kameyama T., Murakami Y., Suto F., Kawakami A., Takagi S., Hirata T., Fujisawa H. Biochem. Biophys. Res. Commun. 226:524-529(1996) [PubMed: 8806667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c and ICR. Tissue: Brain. |
| [2] | "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 495-1894. Tissue: Brain. |
| [3] | "Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors." Takahashi T., Fournier A., Nakamura F., Wang L.-H., Murakami Y., Kalb R.G., Fujisawa H., Strittmatter S.M. Cell 99:59-69(1999) [PubMed: 10520994] [Abstract] Cited for: FUNCTION, INTERACTION WITH NRP1; NRP2; SEMA3A AND SEMA3F. |
| [4] | "Plexin/neuropilin complexes mediate repulsion by the axonal guidance signal semaphorin 3A." Rohm B., Ottemeyer A., Lohrum M., Pueschel A.W. Mech. Dev. 93:95-104(2000) [PubMed: 10781943] [Abstract] Cited for: FUNCTION, INTERACTION WITH NRP1; NRP2; SEMA3A AND SEMA3C. |
| [5] | "Plexin-A1 and plexin-B1 specifically interact at their cytoplasmic domains." Usui H., Taniguchi M., Yokomizo T., Shimizu T. Biochem. Biophys. Res. Commun. 300:927-931(2003) [PubMed: 12559962] [Abstract] Cited for: INTERACTION WITH PLXNB1, TISSUE SPECIFICITY. |
| [6] | "Structural bases for CRMP function in plexin-dependent semaphorin3A signaling." Deo R.C., Schmidt E.F., Elhabazi A., Togashi H., Burley S.K., Strittmatter S.M. EMBO J. 23:9-22(2004) [PubMed: 14685275] [Abstract] Cited for: INTERACTION WITH CRMP1; DPYSL2/CRMP2; DPYSL3/CRMP3 AND DPYSL4/CRMP4. |
| [7] | "Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2." Toyofuku T., Zhang H., Kumanogoh A., Takegahara N., Suto F., Kamei J., Aoki K., Yabuki M., Hori M., Fujisawa H., Kikutani H. Genes Dev. 18:435-447(2004) [PubMed: 14977921] [Abstract] Cited for: INTERACTION WITH SEMA6D AND KDR. |
| [8] | "FARP2 triggers signals for Sema3A-mediated axonal repulsion." Toyofuku T., Yoshida J., Sugimoto T., Zhang H., Kumanogoh A., Hori M., Kikutani H. Nat. Neurosci. 8:1712-1719(2005) [PubMed: 16286926] [Abstract] Cited for: INTERACTION WITH FARP2 AND RND1, MUTAGENESIS OF 1266-LYS--LYS-1268. |
| [9] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-120, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D86948 mRNA. Translation: BAA13188.1. AK220460 mRNA. Translation: BAD90489.1. | |
| IPI | IPI00137311. |
| PIR | JC4980. |
| RefSeq | NP_032907.1. |
| UniGene | Mm.3789 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P70206. |
Proteomic databases | |
| PRIDE | P70206. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000030084. Mus musculus. [Contig view] |
| GeneID | 18844. |
| KEGG | mmu:18844. |
Organism-specific databases | |
| MGI | MGI:107685. Plxna1. |
| Rouge | Search... |
Phylogenomic databases | |
| HOGENOM | P70206. |
| HOVERGEN | P70206. |
| OMA | P70206. CELCLGS. |
Gene expression databases | |
| ArrayExpress | P70206. |
| Bgee | P70206. |
| CleanEx | MM_PLXNA1. |
| GermOnline | ENSMUSG00000030084. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002909. IPT_TIG_rcpt. IPR003659. Plexin-like. IPR013548. Plexin_cytopl. IPR002165. Plexin_repeat. IPR001627. Semaphorin/CD100_Ag. IPR015943. WD40/YVTN_repeat-like. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| Pfam | PF08337. Plexin_cytopl. 1 hit. PF01437. PSI. 3 hits. PF01403. Sema. 1 hit. PF01833. TIG. 4 hits. [Graphical view] |
| SMART | SM00429. IPT. 4 hits. SM00423. PSI. 3 hits. SM00630. Sema. 1 hit. [Graphical view] |
| PROSITE | PS51004. SEMA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 295216. |
| SOURCE | Search... |
Entry information
| Entry name | PLXA1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P70206 Secondary accession number(s): Q5DTR0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


