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P70206 (PLXA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plexin-A1

Short name=Plex 1
Short name=Plexin-1
Gene names
Name:Plxna1
Synonyms:Kiaa4053
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1894 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Ref.4 Ref.5

Subunit structure

Interacts directly with NRP1 and NRP2. Interacts with FARP2, RND1 and KDR/VEGFR2. Binding of SEMA3A leads to dissociation of FARP2. Interacts with WITH CRMP1, DPYSL2/CRMP2, DPYSL3/CRMP3 and DPYSL4/CRMP4. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Tissue specificity

Ubiquitous. Ref.1 Ref.6

Sequence similarities

Belongs to the plexin family.

Contains 4 IPT/TIG domains.

Contains 1 Sema domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 18941867Plexin-A1
PRO_0000232746

Regions

Topological domain28 – 12421215Extracellular Potential
Transmembrane1243 – 126321Helical; Potential
Topological domain1264 – 1894631Cytoplasmic Potential
Domain28 – 510483Sema
Domain862 – 95796IPT/TIG 1
Domain959 – 104385IPT/TIG 2
Domain1046 – 1145100IPT/TIG 3
Domain1148 – 123487IPT/TIG 4
Coiled coil1262 – 131554 Potential

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation6581N-linked (GlcNAc...) Potential
Glycosylation6701N-linked (GlcNAc...) Potential
Glycosylation6991N-linked (GlcNAc...) Potential
Glycosylation10411N-linked (GlcNAc...) Ref.10
Glycosylation11851N-linked (GlcNAc...) Potential
Glycosylation12101N-linked (GlcNAc...) Potential
Disulfide bond93 ↔ 102 By similarity
Disulfide bond128 ↔ 136 By similarity
Disulfide bond284 ↔ 405 By similarity
Disulfide bond300 ↔ 356 By similarity
Disulfide bond374 ↔ 393 By similarity
Disulfide bond513 ↔ 530 By similarity
Disulfide bond519 ↔ 561 By similarity
Disulfide bond522 ↔ 539 By similarity
Disulfide bond533 ↔ 545 By similarity
Disulfide bond596 ↔ 615 By similarity

Experimental info

Mutagenesis1266 – 12683KRK → AAA: Loss of interaction with FARP2. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P70206 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: A8E6BB29C6824C94

FASTA1,894211,099
        10         20         30         40         50         60 
MPLPPLSSRT LLLLLLLLLR GVWIAISSPP AGLGPQPAFR TFVASDWGLT HLVVHEQTGE 

        70         80         90        100        110        120 
VYVGAVNRIY KLSGNLTLLR AHVTGPVEDN EKCYPPPSVQ SCPHGLGSTD NVNKLLLLDY 

       130        140        150        160        170        180 
AANRLLACGS ASQGICQFLR LDDLFKLGEP HHRKEHYLSS VREAGSMAGV LIAGPPGQGQ 

       190        200        210        220        230        240 
AKLFVGTPID GKSEYFPTLS SRRLMANEED ADMFGFVYQD EFVSSQLKIP SDTLSKFPAF 

       250        260        270        280        290        300 
DIYYVYSFRS EQFVYYLTLQ LDTQLTSPDA AGEHFFTSKI VRLCVNDPKF YSYVEFPIGC 

       310        320        330        340        350        360 
EQAGVEYRLV QDAYLSRPGQ ALAKQLGLAE DEEVLFTVFA QGQKNRVKPP KESALCLFTL 

       370        380        390        400        410        420 
RAIKEKIKER IQSCYRGEGK LSLPWLLNKE LGCINSPLQI DDDFCGQDFN QPLGGTVTIE 

       430        440        450        460        470        480 
GTPLFVDKED GLTAVAAYDY QGRTVVFAGT RSGRIRKILV DLANPSGRPA LAYESVVAQE 

       490        500        510        520        530        540 
GNPILRDLVL SPNRQYLYAM TEKQVTQVPV ESCVQYTSCE LCLGSRDPHC GWCVLHSICS 

       550        560        570        580        590        600 
RQDACERAEE PQRFASDLLQ CVQLTVQPRN VSVTMSQVPL VLQAWNVPDL SAGVNCSFED 

       610        620        630        640        650        660 
FTETESILED GRIHCHSPSA REVAPITQGQ GDQRVVKLYL KSKETGKKFA SVDFVFYNCS 

       670        680        690        700        710        720 
VHQSCLACVN GSFPCHWCKY RHVCTNNAAD CAFLEGRVNM SEDCPQILPS THIYVPVGVV 

       730        740        750        760        770        780 
KPITLAARNL PQPQSGQRGY ECLFHIPGSP ARVTALRFNS SSLQCQNSSY SYEGNDVSDL 

       790        800        810        820        830        840 
PVNLSVVWNG NFVIDNPQNI QAHLYKCPAL RQSCGLCLKA DPRFECGWCV AERRCSLRHH 

       850        860        870        880        890        900 
CPADSPASWM HAHHGSSRCT DPKILKLSPE TGPRQGGTRL TITGENLGLR FEDVRLGVHV 

       910        920        930        940        950        960 
GKVLCSPVES EYISAEQIVC EIGDASTLRA HDALVEVCVR DCSLHYRALS PKRFTFVTPT 

       970        980        990       1000       1010       1020 
FYRVSPSRGP LSGGTWIGIE GSHLNAGSDV AVSIGGRPCS FSWRNSREIR CLTPPGHTPG 

      1030       1040       1050       1060       1070       1080 
SAPIVININR AQLSNPEVKY NYTEDPTILR IDPEWSINSG GTLLTVTGTN LATVREPRIR 

      1090       1100       1110       1120       1130       1140 
AKYGGIEREN SCMVYNDTTM VCRAPSIDNP KRSPPELGER PDEIGFIMDN VRTLLVLNSS 

      1150       1160       1170       1180       1190       1200 
SFLYYPDPVL EPLSPTGLLE LKPSSPLILK GRNLLPPAPG NSRLNYTVLI GSTPCILTVS 

      1210       1220       1230       1240       1250       1260 
ETQLLCEAPN LTGQHKVTVR AGGFEFSPGM LQVYSDSLLT LPAIVGIGGG GGLLLLVIVA 

      1270       1280       1290       1300       1310       1320 
VLIAYKRKSR DADRTLKRLQ LQMDNLESRV ALECKEAFAE LQTDIHELTS DLDGAGIPFL 

      1330       1340       1350       1360       1370       1380 
DYRTYAMRVL FPGIEDHPVL KEMEVQANVE KSLTLFGQLL TKKHFLLTFI RTLEAQRSFS 

      1390       1400       1410       1420       1430       1440 
MRDRGNVASL IMTALQGEME YATGVLKQLL SDLIEKNLES KNHPKLLLRR TESVAEKMLT 

      1450       1460       1470       1480       1490       1500 
NWFTFLLYKF LKECAGEPLF MLYCAIKQQM EKGPIDAITG EARYSLSEDK LIRQQIDYKT 

      1510       1520       1530       1540       1550       1560 
LTLNCVNPEH ENAPEVPVKG LNCDTVTQVK EKLLDAVYKG VPYSQRPKAG DMDLEWRQGR 

      1570       1580       1590       1600       1610       1620 
MARIILQDED VTTKIDNDWK RLNTLAHYQV TDGSSVALVP KQTSAYNISN SSTFTKSLSR 

      1630       1640       1650       1660       1670       1680 
YESMLRTASS PDSLRSRTPM ITPDLESGTK LWHLVKNHDH LDQREGDRGS KMVSEIYLTR 

      1690       1700       1710       1720       1730       1740 
LLATKGTLQK FVDDLFETIF STAHRGSALP LAIKYMFDFL DEQADKHQIH DSDVRHTWKS 

      1750       1760       1770       1780       1790       1800 
NCLPLRFWVN VIKNPQFVFD IHKNSITDAC LSVVAQTFMD SCSTSEHKLG KDSPSNKLLY 

      1810       1820       1830       1840       1850       1860 
AKDIPNYKSW VERYYADIAK MPAISDQDMS AYLAEQSRLH LSQFNSMSAL HEIYSYIAKY 

      1870       1880       1890 
KDEILVALEK DEQARRQRLR SKLEQVVDTM ALSS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a neuronal cell surface molecule, plexin, in mice."
Kameyama T., Murakami Y., Suto F., Kawakami A., Takagi S., Hirata T., Fujisawa H.
Biochem. Biophys. Res. Commun. 226:524-529(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c and ICR.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 495-1894.
Tissue: Brain.
[4]"Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors."
Takahashi T., Fournier A., Nakamura F., Wang L.-H., Murakami Y., Kalb R.G., Fujisawa H., Strittmatter S.M.
Cell 99:59-69(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NRP1; NRP2; SEMA3A AND SEMA3F.
[5]"Plexin/neuropilin complexes mediate repulsion by the axonal guidance signal semaphorin 3A."
Rohm B., Ottemeyer A., Lohrum M., Pueschel A.W.
Mech. Dev. 93:95-104(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NRP1; NRP2; SEMA3A AND SEMA3C.
[6]"Plexin-A1 and plexin-B1 specifically interact at their cytoplasmic domains."
Usui H., Taniguchi M., Yokomizo T., Shimizu T.
Biochem. Biophys. Res. Commun. 300:927-931(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PLXNB1, TISSUE SPECIFICITY.
[7]"Structural bases for CRMP function in plexin-dependent semaphorin3A signaling."
Deo R.C., Schmidt E.F., Elhabazi A., Togashi H., Burley S.K., Strittmatter S.M.
EMBO J. 23:9-22(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CRMP1; DPYSL2/CRMP2; DPYSL3/CRMP3 AND DPYSL4/CRMP4.
[8]"Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2."
Toyofuku T., Zhang H., Kumanogoh A., Takegahara N., Suto F., Kamei J., Aoki K., Yabuki M., Hori M., Fujisawa H., Kikutani H.
Genes Dev. 18:435-447(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SEMA6D AND KDR.
[9]"FARP2 triggers signals for Sema3A-mediated axonal repulsion."
Toyofuku T., Yoshida J., Sugimoto T., Zhang H., Kumanogoh A., Hori M., Kikutani H.
Nat. Neurosci. 8:1712-1719(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FARP2 AND RND1, MUTAGENESIS OF 1266-LYS--LYS-1268.
[10]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1041.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86948 mRNA. Translation: BAA13188.1.
BC138023 mRNA. Translation: AAI38024.1.
AK220460 mRNA. Translation: BAD90489.1.
CCDSCCDS20344.1.
PIRJC4980.
RefSeqNP_032907.1. NM_008881.2.
UniGeneMm.3789.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RYTX-ray3.58A/B1269-1894[»]
ProteinModelPortalP70206.
SMRP70206. Positions 37-705, 812-923, 1278-1890.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202261. 4 interactions.
DIPDIP-29745N.
IntActP70206. 3 interactions.
MINTMINT-4106183.

PTM databases

PhosphoSiteP70206.

Proteomic databases

MaxQBP70206.
PaxDbP70206.
PRIDEP70206.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049845; ENSMUSP00000063066; ENSMUSG00000030084.
ENSMUST00000163139; ENSMUSP00000131840; ENSMUSG00000030084.
GeneID18844.
KEGGmmu:18844.
UCSCuc009cwg.2. mouse.

Organism-specific databases

CTD5361.
MGIMGI:107685. Plxna1.
RougeSearch...

Phylogenomic databases

eggNOGNOG254546.
GeneTreeENSGT00750000117292.
HOGENOMHOG000231377.
HOVERGENHBG105711.
InParanoidB2RQP7.
KOK06820.
OMAIGCEQAG.
OrthoDBEOG7Q8CM9.
PhylomeDBP70206.
TreeFamTF312962.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressP70206.
BgeeP70206.
CleanExMM_PLXNA1.
GenevestigatorP70206.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.60.40.10. 4 hits.
InterProIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR016201. Plexin-like_fold.
IPR013548. Plexin_cytoplasmic_RasGAP_dom.
IPR002165. Plexin_repeat.
IPR008936. Rho_GTPase_activation_prot.
IPR001627. Semap_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamPF08337. Plexin_cytopl. 1 hit.
PF01437. PSI. 3 hits.
PF01403. Sema. 1 hit.
PF01833. TIG. 4 hits.
[Graphical view]
SMARTSM00429. IPT. 4 hits.
SM00423. PSI. 3 hits.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 2 hits.
SSF48350. SSF48350. 2 hits.
SSF81296. SSF81296. 4 hits.
PROSITEPS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295216.
PROP70206.
SOURCESearch...

Entry information

Entry namePLXA1_MOUSE
AccessionPrimary (citable) accession number: P70206
Secondary accession number(s): B2RQP7, Q5DTR0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot