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P70191

- TRAF5_MOUSE

UniProt

P70191 - TRAF5_MOUSE

Protein

TNF receptor-associated factor 5

Gene

Traf5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri45 – 8541RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri127 – 18155TRAF-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri182 – 23958TRAF-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. signal transducer activity Source: MGI
    3. ubiquitin-protein transferase activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. positive regulation of cell proliferation Source: MGI
    3. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: InterPro
    4. positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
    5. regulation of apoptotic process Source: InterPro
    6. signal transduction Source: MGI

    Keywords - Biological processi

    Apoptosis

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TNF receptor-associated factor 5
    Gene namesi
    Name:Traf5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:107548. Traf5.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytosol

    GO - Cellular componenti

    1. CD40 receptor complex Source: BHF-UCL
    2. cytoplasmic side of plasma membrane Source: BHF-UCL
    3. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 558558TNF receptor-associated factor 5PRO_0000056406Add
    BLAST

    Proteomic databases

    PRIDEiP70191.

    PTM databases

    PhosphoSiteiP70191.

    Expressioni

    Gene expression databases

    CleanExiMM_TRAF5.
    GenevestigatoriP70191.

    Interactioni

    Subunit structurei

    Homotrimer Probable. Heterotrimer with TRAF3 By similarity. Associates with TNFRSF5/CD40 through interaction with TRAF3 By similarity. Associates with LTBR/TNFRSF3, TNFRSF4, TNFRSF8/CD30, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF17, TNFRSF19/TROY, RIPK2, MAP3K14, MAP3K5, and TRAF and TNF receptor associated protein TDP2 By similarity. Interacts (via C-terminus) with EIF2AK2/PKR (via the kinase catalytic domain) By similarity.By similarityCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COG7P834362EBI-523899,EBI-389534From a different organism.
    Srsf1Q6PDM22EBI-523899,EBI-2550360
    TRAF2Q129332EBI-523899,EBI-355744From a different organism.
    Traf3ip2Q8N7N63EBI-523899,EBI-646165

    Protein-protein interaction databases

    BioGridi204306. 4 interactions.
    IntActiP70191. 13 interactions.

    Structurei

    Secondary structure

    1
    558
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi384 – 39916
    Beta strandi402 – 4109
    Helixi413 – 4219
    Beta strandi433 – 4364
    Beta strandi441 – 4477
    Helixi452 – 4543
    Turni455 – 4573
    Beta strandi458 – 4669
    Helixi471 – 4733
    Beta strandi481 – 4866
    Beta strandi496 – 5016
    Helixi507 – 5093
    Beta strandi513 – 5164
    Beta strandi520 – 5278
    Helixi528 – 5325
    Beta strandi538 – 5403
    Beta strandi543 – 5508

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GJHX-ray2.80A/B/C381-558[»]
    ProteinModelPortaliP70191.
    SMRiP70191. Positions 32-189, 347-557.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini403 – 550148MATHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni345 – 558214Interaction with EIF2AK2/PKRBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili252 – 30251Sequence AnalysisAdd
    BLAST
    Coiled coili340 – 40061Sequence AnalysisAdd
    BLAST

    Domaini

    The MATH/TRAF domain binds to receptor cytoplasmic domains.

    Sequence similaritiesi

    Contains 1 MATH domain.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 2 TRAF-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri45 – 8541RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri127 – 18155TRAF-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri182 – 23958TRAF-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG239194.
    HOGENOMiHOG000231557.
    HOVERGENiHBG058222.
    InParanoidiP70191.
    KOiK09849.
    PhylomeDBiP70191.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    3.90.890.10. 2 hits.
    InterProiIPR002083. MATH.
    IPR013323. SIAH-type.
    IPR012227. TNF_rcpt--assoc_TRAF.
    IPR008974. TRAF-like.
    IPR027130. TRAF5.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    IPR001293. Znf_TRAF.
    [Graphical view]
    PANTHERiPTHR10131:SF66. PTHR10131:SF66. 1 hit.
    PfamiPF00917. MATH. 1 hit.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015614. TRAF. 1 hit.
    SMARTiSM00061. MATH. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF49599. SSF49599. 3 hits.
    PROSITEiPS50144. MATH. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    PS50145. ZF_TRAF. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70191-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHSEEQAAV PCAFIRQNSG NSISLDFEPD TEYQFVEQLE ERYKCAFCHS    50
    VLHNPHQTGC GHRFCQQCIR SLRELNSVPI CPVDKEVIKP QEVFKDNCCK 100
    REVLNLHVYC KNAPGCNARI ILGRFQDHLQ HCSFQAVPCP NESCREAMLR 150
    KDVKEHLSAY CRFREEKCLY CKRDIVVTNL QDHEENSCPA YPVSCPNRCV 200
    QTIPRARVNE HLTVCPEAEQ DCPFKHYGCT VKGKRGNLLE HERAALQDHM 250
    LLVLEKNYQL EQRISDLYQS LEQKESKIQQ LAETVKKFEK ELKQFTQMFG 300
    RNGTFLSNVQ ALTSHTDKSA WLEAQVRQLL QIVNQQPSRL DLRSLVDAVD 350
    SVKQRITQLE ASDQRLVLLE GETSKHDAHI NIHKAQLNKN EERFKQLEGA 400
    CYSGKLIWKV TDYRVKKREA VEGHTVSVFS QPFYTSRCGY RLCARAYLNG 450
    DGSGKGTHLS LYFVVMRGEF DSLLQWPFRQ RVTLMLLDQS GKKNHIVETF 500
    KADPNSSSFK RPDGEMNIAS GCPRFVSHST LENSKNTYIK DDTLFLKVAV 550
    DLTDLEDL 558
    Length:558
    Mass (Da):64,145
    Last modified:February 1, 1997 - v1
    Checksum:i48F64FB83BD587E5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti328 – 3281Q → H in BAA11218. (PubMed:8663299)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78141 mRNA. Translation: BAA11218.1.
    D83528 mRNA. Translation: BAA11942.1.
    BC012702 mRNA. Translation: AAH12702.1.
    RefSeqiNP_035763.2. NM_011633.2.
    UniGeneiMm.380491.
    Mm.389227.

    Genome annotation databases

    GeneIDi22033.
    KEGGimmu:22033.
    UCSCiuc029qvg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78141 mRNA. Translation: BAA11218.1 .
    D83528 mRNA. Translation: BAA11942.1 .
    BC012702 mRNA. Translation: AAH12702.1 .
    RefSeqi NP_035763.2. NM_011633.2.
    UniGenei Mm.380491.
    Mm.389227.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GJH X-ray 2.80 A/B/C 381-558 [» ]
    ProteinModelPortali P70191.
    SMRi P70191. Positions 32-189, 347-557.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204306. 4 interactions.
    IntActi P70191. 13 interactions.

    PTM databases

    PhosphoSitei P70191.

    Proteomic databases

    PRIDEi P70191.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 22033.
    KEGGi mmu:22033.
    UCSCi uc029qvg.1. mouse.

    Organism-specific databases

    CTDi 7188.
    MGIi MGI:107548. Traf5.

    Phylogenomic databases

    eggNOGi NOG239194.
    HOGENOMi HOG000231557.
    HOVERGENi HBG058222.
    InParanoidi P70191.
    KOi K09849.
    PhylomeDBi P70191.

    Miscellaneous databases

    NextBioi 301794.
    PROi P70191.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_TRAF5.
    Genevestigatori P70191.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    3.90.890.10. 2 hits.
    InterProi IPR002083. MATH.
    IPR013323. SIAH-type.
    IPR012227. TNF_rcpt--assoc_TRAF.
    IPR008974. TRAF-like.
    IPR027130. TRAF5.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    IPR001293. Znf_TRAF.
    [Graphical view ]
    PANTHERi PTHR10131:SF66. PTHR10131:SF66. 1 hit.
    Pfami PF00917. MATH. 1 hit.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015614. TRAF. 1 hit.
    SMARTi SM00061. MATH. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49599. SSF49599. 3 hits.
    PROSITEi PS50144. MATH. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    PS50145. ZF_TRAF. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TRAF5, an activator of NF-kappaB and putative signal transducer for the lymphotoxin-beta receptor."
      Nakano H., Oshima H., Chung W., Williams-Abbott L., Ware C.F., Yagita H., Okumura K.
      J. Biol. Chem. 271:14661-14664(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH LTBR.
      Strain: BALB/c.
    2. "TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling."
      Ishida T., Tojo T., Aoki T., Kobayashi N., Ohishi T., Watanabe T., Yamamoto T., Inoue J.
      Proc. Natl. Acad. Sci. U.S.A. 93:9437-9442(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TNFRSF5.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    4. Cited for: FUNCTION.

    Entry informationi

    Entry nameiTRAF5_MOUSE
    AccessioniPrimary (citable) accession number: P70191
    Secondary accession number(s): Q61480
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3