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Protein

TNF receptor-associated factor 5

Gene

Traf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri45 – 8541RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri127 – 18155TRAF-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri182 – 23958TRAF-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: InterPro
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • regulation of apoptotic process Source: InterPro
  • signal transduction Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TNF receptor-associated factor 5
Gene namesi
Name:Traf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107548. Traf5.

Subcellular locationi

GO - Cellular componenti

  • CD40 receptor complex Source: BHF-UCL
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic side of plasma membrane Source: BHF-UCL
  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558TNF receptor-associated factor 5PRO_0000056406Add
BLAST

Proteomic databases

MaxQBiP70191.
PRIDEiP70191.

PTM databases

PhosphoSiteiP70191.

Expressioni

Gene expression databases

CleanExiMM_TRAF5.
GenevisibleiP70191. MM.

Interactioni

Subunit structurei

Homotrimer (Probable). Heterotrimer with TRAF3 (By similarity). Associates with TNFRSF5/CD40 through interaction with TRAF3 (By similarity). Associates with LTBR/TNFRSF3, TNFRSF4, TNFRSF8/CD30, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF17, TNFRSF19/TROY, RIPK2, MAP3K14, MAP3K5, and TRAF and TNF receptor associated protein TDP2 (By similarity). Interacts (via C-terminus) with EIF2AK2/PKR (via the kinase catalytic domain) (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
COG7P834362EBI-523899,EBI-389534From a different organism.
Srsf1Q6PDM22EBI-523899,EBI-2550360
TRAF2Q129332EBI-523899,EBI-355744From a different organism.
Traf3ip2Q8N7N63EBI-523899,EBI-646165

Protein-protein interaction databases

BioGridi204306. 4 interactions.
IntActiP70191. 13 interactions.

Structurei

Secondary structure

1
558
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi384 – 39916Combined sources
Beta strandi402 – 4109Combined sources
Helixi413 – 4219Combined sources
Beta strandi433 – 4364Combined sources
Beta strandi441 – 4477Combined sources
Helixi452 – 4543Combined sources
Turni455 – 4573Combined sources
Beta strandi458 – 4669Combined sources
Helixi471 – 4733Combined sources
Beta strandi481 – 4866Combined sources
Beta strandi496 – 5016Combined sources
Helixi507 – 5093Combined sources
Beta strandi513 – 5164Combined sources
Beta strandi520 – 5278Combined sources
Helixi528 – 5325Combined sources
Beta strandi538 – 5403Combined sources
Beta strandi543 – 5508Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GJHX-ray2.80A/B/C381-558[»]
ProteinModelPortaliP70191.
SMRiP70191. Positions 32-189, 347-557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini403 – 550148MATHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 558214Interaction with EIF2AK2/PKRBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili252 – 30251Sequence AnalysisAdd
BLAST
Coiled coili340 – 40061Sequence AnalysisAdd
BLAST

Domaini

The MATH/TRAF domain binds to receptor cytoplasmic domains.

Sequence similaritiesi

Contains 1 MATH domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 2 TRAF-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri45 – 8541RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri127 – 18155TRAF-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri182 – 23958TRAF-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG239194.
HOGENOMiHOG000231557.
HOVERGENiHBG058222.
InParanoidiP70191.
KOiK09849.
PhylomeDBiP70191.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.90.890.10. 2 hits.
InterProiIPR002083. MATH.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR027130. TRAF5.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR10131:SF66. PTHR10131:SF66. 1 hit.
PfamiPF00917. MATH. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 3 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSEEQAAV PCAFIRQNSG NSISLDFEPD TEYQFVEQLE ERYKCAFCHS
60 70 80 90 100
VLHNPHQTGC GHRFCQQCIR SLRELNSVPI CPVDKEVIKP QEVFKDNCCK
110 120 130 140 150
REVLNLHVYC KNAPGCNARI ILGRFQDHLQ HCSFQAVPCP NESCREAMLR
160 170 180 190 200
KDVKEHLSAY CRFREEKCLY CKRDIVVTNL QDHEENSCPA YPVSCPNRCV
210 220 230 240 250
QTIPRARVNE HLTVCPEAEQ DCPFKHYGCT VKGKRGNLLE HERAALQDHM
260 270 280 290 300
LLVLEKNYQL EQRISDLYQS LEQKESKIQQ LAETVKKFEK ELKQFTQMFG
310 320 330 340 350
RNGTFLSNVQ ALTSHTDKSA WLEAQVRQLL QIVNQQPSRL DLRSLVDAVD
360 370 380 390 400
SVKQRITQLE ASDQRLVLLE GETSKHDAHI NIHKAQLNKN EERFKQLEGA
410 420 430 440 450
CYSGKLIWKV TDYRVKKREA VEGHTVSVFS QPFYTSRCGY RLCARAYLNG
460 470 480 490 500
DGSGKGTHLS LYFVVMRGEF DSLLQWPFRQ RVTLMLLDQS GKKNHIVETF
510 520 530 540 550
KADPNSSSFK RPDGEMNIAS GCPRFVSHST LENSKNTYIK DDTLFLKVAV

DLTDLEDL
Length:558
Mass (Da):64,145
Last modified:February 1, 1997 - v1
Checksum:i48F64FB83BD587E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281Q → H in BAA11218 (PubMed:8663299).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78141 mRNA. Translation: BAA11218.1.
D83528 mRNA. Translation: BAA11942.1.
BC012702 mRNA. Translation: AAH12702.1.
RefSeqiNP_035763.2. NM_011633.2.
UniGeneiMm.380491.
Mm.389227.

Genome annotation databases

GeneIDi22033.
KEGGimmu:22033.
UCSCiuc029qvg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78141 mRNA. Translation: BAA11218.1.
D83528 mRNA. Translation: BAA11942.1.
BC012702 mRNA. Translation: AAH12702.1.
RefSeqiNP_035763.2. NM_011633.2.
UniGeneiMm.380491.
Mm.389227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GJHX-ray2.80A/B/C381-558[»]
ProteinModelPortaliP70191.
SMRiP70191. Positions 32-189, 347-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204306. 4 interactions.
IntActiP70191. 13 interactions.

PTM databases

PhosphoSiteiP70191.

Proteomic databases

MaxQBiP70191.
PRIDEiP70191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22033.
KEGGimmu:22033.
UCSCiuc029qvg.2. mouse.

Organism-specific databases

CTDi7188.
MGIiMGI:107548. Traf5.

Phylogenomic databases

eggNOGiNOG239194.
HOGENOMiHOG000231557.
HOVERGENiHBG058222.
InParanoidiP70191.
KOiK09849.
PhylomeDBiP70191.

Miscellaneous databases

NextBioi301794.
PROiP70191.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TRAF5.
GenevisibleiP70191. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.90.890.10. 2 hits.
InterProiIPR002083. MATH.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR027130. TRAF5.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR10131:SF66. PTHR10131:SF66. 1 hit.
PfamiPF00917. MATH. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 3 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TRAF5, an activator of NF-kappaB and putative signal transducer for the lymphotoxin-beta receptor."
    Nakano H., Oshima H., Chung W., Williams-Abbott L., Ware C.F., Yagita H., Okumura K.
    J. Biol. Chem. 271:14661-14664(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH LTBR.
    Strain: BALB/c.
  2. "TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling."
    Ishida T., Tojo T., Aoki T., Kobayashi N., Ohishi T., Watanabe T., Yamamoto T., Inoue J.
    Proc. Natl. Acad. Sci. U.S.A. 93:9437-9442(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TNFRSF5.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  4. Cited for: FUNCTION.

Entry informationi

Entry nameiTRAF5_MOUSE
AccessioniPrimary (citable) accession number: P70191
Secondary accession number(s): Q61480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: February 1, 1997
Last modified: July 22, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.