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Protein

TNF receptor-associated factor 5

Gene

Traf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri45 – 85RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri127 – 181TRAF-type 1PROSITE-ProRule annotationAdd BLAST55
Zinc fingeri182 – 239TRAF-type 2PROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: InterPro
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • regulation of apoptotic process Source: InterPro
  • signal transduction Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TNF receptor-associated factor 5
Gene namesi
Name:Traf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107548. Traf5.

Subcellular locationi

GO - Cellular componenti

  • CD40 receptor complex Source: BHF-UCL
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic side of plasma membrane Source: BHF-UCL
  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000564061 – 558TNF receptor-associated factor 5Add BLAST558

Proteomic databases

MaxQBiP70191.
PRIDEiP70191.

PTM databases

iPTMnetiP70191.
PhosphoSitePlusiP70191.

Expressioni

Gene expression databases

CleanExiMM_TRAF5.

Interactioni

Subunit structurei

Homotrimer (Probable). Heterotrimer with TRAF3 (By similarity). Associates with TNFRSF5/CD40 through interaction with TRAF3 (By similarity). Associates with LTBR/TNFRSF3, TNFRSF4, TNFRSF8/CD30, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF17, TNFRSF19/TROY, RIPK2, MAP3K14, MAP3K5, and TRAF and TNF receptor associated protein TDP2 (By similarity). Interacts (via C-terminus) with EIF2AK2/PKR (via the kinase catalytic domain) (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
COG7P834362EBI-523899,EBI-389534From a different organism.
Srsf1Q6PDM22EBI-523899,EBI-2550360
TRAF2Q129332EBI-523899,EBI-355744From a different organism.
Traf3ip2Q8N7N63EBI-523899,EBI-646165

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204306. 4 interactors.
DIPiDIP-34999N.
IntActiP70191. 13 interactors.

Structurei

Secondary structure

1558
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi384 – 399Combined sources16
Beta strandi402 – 410Combined sources9
Helixi413 – 421Combined sources9
Beta strandi433 – 436Combined sources4
Beta strandi441 – 447Combined sources7
Helixi452 – 454Combined sources3
Turni455 – 457Combined sources3
Beta strandi458 – 466Combined sources9
Helixi471 – 473Combined sources3
Beta strandi481 – 486Combined sources6
Beta strandi496 – 501Combined sources6
Helixi507 – 509Combined sources3
Beta strandi513 – 516Combined sources4
Beta strandi520 – 527Combined sources8
Helixi528 – 532Combined sources5
Beta strandi538 – 540Combined sources3
Beta strandi543 – 550Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GJHX-ray2.80A/B/C381-558[»]
ProteinModelPortaliP70191.
SMRiP70191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini403 – 550MATHPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 558Interaction with EIF2AK2/PKRBy similarityAdd BLAST214

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili252 – 302Sequence analysisAdd BLAST51
Coiled coili340 – 400Sequence analysisAdd BLAST61

Domaini

The MATH/TRAF domain binds to receptor cytoplasmic domains.

Sequence similaritiesi

Contains 1 MATH domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 2 TRAF-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri45 – 85RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri127 – 181TRAF-type 1PROSITE-ProRule annotationAdd BLAST55
Zinc fingeri182 – 239TRAF-type 2PROSITE-ProRule annotationAdd BLAST58

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000231557.
HOVERGENiHBG058222.
InParanoidiP70191.
KOiK09849.
PhylomeDBiP70191.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
3.30.40.10. 1 hit.
3.90.890.10. 3 hits.
InterProiIPR002083. MATH/TRAF_dom.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR027130. TRAF5.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR10131:SF83. PTHR10131:SF83. 1 hit.
PfamiPF00097. zf-C3HC4. 1 hit.
PF02176. zf-TRAF. 1 hit.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 3 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSEEQAAV PCAFIRQNSG NSISLDFEPD TEYQFVEQLE ERYKCAFCHS
60 70 80 90 100
VLHNPHQTGC GHRFCQQCIR SLRELNSVPI CPVDKEVIKP QEVFKDNCCK
110 120 130 140 150
REVLNLHVYC KNAPGCNARI ILGRFQDHLQ HCSFQAVPCP NESCREAMLR
160 170 180 190 200
KDVKEHLSAY CRFREEKCLY CKRDIVVTNL QDHEENSCPA YPVSCPNRCV
210 220 230 240 250
QTIPRARVNE HLTVCPEAEQ DCPFKHYGCT VKGKRGNLLE HERAALQDHM
260 270 280 290 300
LLVLEKNYQL EQRISDLYQS LEQKESKIQQ LAETVKKFEK ELKQFTQMFG
310 320 330 340 350
RNGTFLSNVQ ALTSHTDKSA WLEAQVRQLL QIVNQQPSRL DLRSLVDAVD
360 370 380 390 400
SVKQRITQLE ASDQRLVLLE GETSKHDAHI NIHKAQLNKN EERFKQLEGA
410 420 430 440 450
CYSGKLIWKV TDYRVKKREA VEGHTVSVFS QPFYTSRCGY RLCARAYLNG
460 470 480 490 500
DGSGKGTHLS LYFVVMRGEF DSLLQWPFRQ RVTLMLLDQS GKKNHIVETF
510 520 530 540 550
KADPNSSSFK RPDGEMNIAS GCPRFVSHST LENSKNTYIK DDTLFLKVAV

DLTDLEDL
Length:558
Mass (Da):64,145
Last modified:February 1, 1997 - v1
Checksum:i48F64FB83BD587E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328Q → H in BAA11218 (PubMed:8663299).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78141 mRNA. Translation: BAA11218.1.
D83528 mRNA. Translation: BAA11942.1.
BC012702 mRNA. Translation: AAH12702.1.
RefSeqiNP_035763.2. NM_011633.2.
UniGeneiMm.380491.
Mm.389227.

Genome annotation databases

GeneIDi22033.
KEGGimmu:22033.
UCSCiuc029qvg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78141 mRNA. Translation: BAA11218.1.
D83528 mRNA. Translation: BAA11942.1.
BC012702 mRNA. Translation: AAH12702.1.
RefSeqiNP_035763.2. NM_011633.2.
UniGeneiMm.380491.
Mm.389227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GJHX-ray2.80A/B/C381-558[»]
ProteinModelPortaliP70191.
SMRiP70191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204306. 4 interactors.
DIPiDIP-34999N.
IntActiP70191. 13 interactors.

PTM databases

iPTMnetiP70191.
PhosphoSitePlusiP70191.

Proteomic databases

MaxQBiP70191.
PRIDEiP70191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22033.
KEGGimmu:22033.
UCSCiuc029qvg.2. mouse.

Organism-specific databases

CTDi7188.
MGIiMGI:107548. Traf5.

Phylogenomic databases

HOGENOMiHOG000231557.
HOVERGENiHBG058222.
InParanoidiP70191.
KOiK09849.
PhylomeDBiP70191.

Miscellaneous databases

PROiP70191.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TRAF5.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
3.30.40.10. 1 hit.
3.90.890.10. 3 hits.
InterProiIPR002083. MATH/TRAF_dom.
IPR013323. SIAH-type.
IPR012227. TNF_rcpt--assoc_TRAF.
IPR008974. TRAF-like.
IPR027130. TRAF5.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR10131:SF83. PTHR10131:SF83. 1 hit.
PfamiPF00097. zf-C3HC4. 1 hit.
PF02176. zf-TRAF. 1 hit.
[Graphical view]
PIRSFiPIRSF015614. TRAF. 1 hit.
SMARTiSM00061. MATH. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 3 hits.
PROSITEiPS50144. MATH. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
PS50145. ZF_TRAF. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAF5_MOUSE
AccessioniPrimary (citable) accession number: P70191
Secondary accession number(s): Q61480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.