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Protein

Epiphycan

Gene

Epyc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization.

Names & Taxonomyi

Protein namesi
Recommended name:
Epiphycan
Alternative name(s):
Dermatan sulfate proteoglycan 3
Proteoglycan-Lb
Short name:
PG-Lb
Small chondroitin/dermatan sulfate proteoglycan
Gene namesi
Name:Epyc
Synonyms:Dspg3, Pglb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107942. Epyc.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 322303EpiphycanPRO_0000032769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641O-linked (Xyl...) (dermatan sulfate)By similarity
Glycosylationi96 – 961O-linked (GalNAc...)By similarity
Disulfide bondi118 ↔ 130By similarity
Disulfide bondi279 ↔ 312By similarity
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The O-linked polysaccharide on Ser-96 is probably the mucin type linked to GalNAc. There is one glycosaminoglycan chain, known to be dermatan sulfate, and it is probably the O-glycosylation at Ser-64 (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiP70186.
PRIDEiP70186.

PTM databases

iPTMnetiP70186.
PhosphoSiteiP70186.

Expressioni

Tissue specificityi

Confined to the middle zone of embryonic epiphyseal cartilage consisting of flattened chondrocytes and the ossifying region in the limb buds of chick embryos. Has also been detected in testis.1 Publication

Developmental stagei

Expression starts at E12.5 dpc and is restricted to developing cartilage.

Gene expression databases

BgeeiP70186.
CleanExiMM_EPYC.
GenevisibleiP70186. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020094.

Structurei

3D structure databases

ProteinModelPortaliP70186.
SMRiP70186. Positions 111-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 14338LRRNTAdd
BLAST
Repeati144 – 16522LRR 1Add
BLAST
Repeati168 – 18922LRR 2Add
BLAST
Repeati192 – 21322LRR 3Add
BLAST
Repeati238 – 25821LRR 4Add
BLAST
Repeati259 – 28022LRR 5Add
BLAST
Repeati290 – 31021LRR 6Add
BLAST

Sequence similaritiesi

Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00730000110781.
HOGENOMiHOG000273909.
HOVERGENiHBG006850.
InParanoidiP70186.
KOiK08127.
OMAiCDDHELD.
OrthoDBiEOG757CXS.
PhylomeDBiP70186.
TreeFamiTF351924.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR027217. Epiphycan.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24373:SF123. PTHR24373:SF123. 1 hit.
PfamiPF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMLARVALG LIIIDAVLAA PTTELFNYDS EVYDAILEDT GTFYNYEHIP
60 70 80 90 100
DNHVENEKVS ERLSGNRELL TPGPQLGDNQ DEDKDEESTP RLIDGSSPQE
110 120 130 140 150
PEFPGLLGPH TNEDFPTCLL CTCISTTVYC DDHELDAIPP LPKKTTYFYS
160 170 180 190 200
RFNRIKKINK NDFASLNDLK RIDLTSNLIS EIDEDAFRKL PHLQELVLRD
210 220 230 240 250
NKIKQLPELP NTLTFIDISN NRLGRKGIKQ EAFKDMYDLH HLYITDNSLD
260 270 280 290 300
HIPLPLPESL RALHLQNNDI LEMHEDTFCN VKNLTYVRKA LEDIRLDGNP
310 320
INLSRTPQAY MCLPRLPIGS FI
Length:322
Mass (Da):36,763
Last modified:February 1, 1997 - v1
Checksum:iC9C8CBEBECDAC19A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78274 mRNA. Translation: BAA11337.1.
D87187 Genomic DNA. Translation: BAA28770.1.
BC012695 mRNA. Translation: AAH12695.1.
AK015223 mRNA. Translation: BAB29756.1.
CCDSiCCDS24143.1.
PIRiS72271.
RefSeqiNP_031910.1. NM_007884.2.
XP_006513266.1. XM_006513203.2.
UniGeneiMm.148886.

Genome annotation databases

EnsembliENSMUST00000020094; ENSMUSP00000020094; ENSMUSG00000019936.
ENSMUST00000105285; ENSMUSP00000100922; ENSMUSG00000019936.
GeneIDi13516.
KEGGimmu:13516.
UCSCiuc007gxb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78274 mRNA. Translation: BAA11337.1.
D87187 Genomic DNA. Translation: BAA28770.1.
BC012695 mRNA. Translation: AAH12695.1.
AK015223 mRNA. Translation: BAB29756.1.
CCDSiCCDS24143.1.
PIRiS72271.
RefSeqiNP_031910.1. NM_007884.2.
XP_006513266.1. XM_006513203.2.
UniGeneiMm.148886.

3D structure databases

ProteinModelPortaliP70186.
SMRiP70186. Positions 111-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020094.

PTM databases

iPTMnetiP70186.
PhosphoSiteiP70186.

Proteomic databases

PaxDbiP70186.
PRIDEiP70186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020094; ENSMUSP00000020094; ENSMUSG00000019936.
ENSMUST00000105285; ENSMUSP00000100922; ENSMUSG00000019936.
GeneIDi13516.
KEGGimmu:13516.
UCSCiuc007gxb.1. mouse.

Organism-specific databases

CTDi1833.
MGIiMGI:107942. Epyc.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00730000110781.
HOGENOMiHOG000273909.
HOVERGENiHBG006850.
InParanoidiP70186.
KOiK08127.
OMAiCDDHELD.
OrthoDBiEOG757CXS.
PhylomeDBiP70186.
TreeFamiTF351924.

Miscellaneous databases

PROiP70186.
SOURCEiSearch...

Gene expression databases

BgeeiP70186.
CleanExiMM_EPYC.
GenevisibleiP70186. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR027217. Epiphycan.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24373:SF123. PTHR24373:SF123. 1 hit.
PfamiPF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Occurrence of PG-Lb, a leucine-rich small chondroitin/dermatan sulphate proteoglycan in mammalian epiphyseal cartilage: molecular cloning and sequence analysis of the mouse cDNA."
    Kurita K., Shinomura T., Ujita M., Zako M., Kida D., Iwata H., Kimata K.
    Biochem. J. 318:909-914(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Epiphyseal cartilage.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "The gene structure and organization of mouse PG-Lb, a small chondroitin/dermatan sulphate proteoglycan."
    Iwata Y., Shinomura T., Kurita K., Zako M., Kimata K.
    Biochem. J. 331:959-964(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
    Strain: 129/Sv.
  5. "Expression and localization of PG-Lb/epiphycan during mouse development."
    Johnson H.J., Shinomura T., Eberspaecher H., Pinero G., Decrombrugghe B., Hoeoek M.
    Dev. Dyn. 216:499-510(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPRESSION DURING DEVELOPMENT.

Entry informationi

Entry nameiEPYC_MOUSE
AccessioniPrimary (citable) accession number: P70186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.