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Protein

Atrial natriuretic peptide receptor 3

Gene

Npr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ChlorideBy similarity
Binding sitei130 – 1301Chloride; via amide nitrogenBy similarity
Binding sitei131 – 1311Chloride; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: Ensembl
  • negative regulation of smooth muscle cell proliferation Source: Ensembl
  • osteoclast proliferation Source: UniProtKB
  • pancreatic juice secretion Source: Ensembl
  • phosphatidylinositol-mediated signaling Source: Ensembl
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  • positive regulation of nitric-oxide synthase activity Source: Ensembl
  • positive regulation of urine volume Source: UniProtKB
  • regulation of blood pressure Source: UniProtKB
  • regulation of osteoblast proliferation Source: UniProtKB
  • skeletal system development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 3
Alternative name(s):
Atrial natriuretic peptide clearance receptor
Atrial natriuretic peptide receptor type C
Short name:
ANP-C
Short name:
ANPR-C
Short name:
NPR-C
EF-2
Gene namesi
Name:Npr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97373. Npr3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 478438ExtracellularSequence analysisAdd
BLAST
Transmembranei479 – 49921HelicalSequence analysisAdd
BLAST
Topological domaini500 – 53637CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Npr3 are the cause of a number of skeletal-overgrowth phenotypes, Longjohn (Lgj), Longjohn-2J (Lgj-2J) and Strigosus (Stri). These are all recessive conditions characterized by an elongated body, thoracic kyphosis, arachnodactyly, and sacral and/or tail kinks, but no significant changes in craniofacial structures (PubMed:10468599).

Disruption phenotypei

Half of the mice dies before weaning. They display reduced ability to concentrate urine, a lower blood pressure, and skeletal abnormalities. Despite a reduced half-life of NPPA in the circulation, the plasma levels of NPPA AND NPPB are not affected.1 Publication

Keywords - Diseasei

Disease mutation

Chemistry

ChEMBLiCHEMBL4711.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Propeptidei27 – 4014Sequence analysisPRO_0000012371Add
BLAST
Chaini41 – 536496Atrial natriuretic peptide receptor 3PRO_0000012372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi103 ↔ 131By similarity
Disulfide bondi208 ↔ 256By similarity
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi468 – 468InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP70180.
PaxDbiP70180.
PRIDEiP70180.

PTM databases

PhosphoSiteiP70180.

Expressioni

Gene expression databases

CleanExiMM_NPR3.
GenevisibleiP70180. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

DIPiDIP-46248N.
IntActiP70180. 1 interaction.
MINTiMINT-4087811.
STRINGi10090.ENSMUSP00000066737.

Chemistry

BindingDBiP70180.

Structurei

3D structure databases

ProteinModelPortaliP70180.
SMRiP70180. Positions 41-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ANF receptor family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00440000033872.
HOGENOMiHOG000236252.
HOVERGENiHBG060145.
InParanoidiP70180.
KOiK12325.
OMAiFQVAYED.
OrthoDBiEOG7BZVS0.
TreeFamiTF106339.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLLLFTFS ACVLLARVLL AGGASSGAGD TRPGSRRRAR EALAAQKIEV
60 70 80 90 100
LVLLPRDDSY LFSLARVRPA IEYALRSVEG NGTGRKLLPP GTRFQVAYED
110 120 130 140 150
SDCGNRALFS LVDRVAAARG AKPDLILGPV CEYAAAPVAR LASHWDLPML
160 170 180 190 200
SAGALAAGFQ HKDTEYSHLT RVAPAYAKMG EMMLALFRHH HWSRAALVYS
210 220 230 240 250
DDKLERNCYF TLEGVHEVFQ EEGLHTSAYN FDETKDLDLD DIVRYIQGSE
260 270 280 290 300
RVVIMCASGD TIRRIMLAVH RHGMTSGDYA FFNIELFNSS SYGDGSWRRG
310 320 330 340 350
DKHDSEAKQA YSSLQTVTLL RTVKPEFEKF SMEVKSSVEK QGLNEEDYVN
360 370 380 390 400
MFVEGFHDAI LLYVLALHEV LRAGYSKKDG GKIIQQTWNR TFEGIAGQVS
410 420 430 440 450
IDANGDRYGD FSVVAMTDTE AGTQEVIGDY FGKEGRFQMR SNVKYPWGPL
460 470 480 490 500
KLRLDETRIV EHTNSSPCKS SGGLEESAVT GIVVGALLGA GLLMAFYFFR
510 520 530
KKYRITIERR NQQEESNIGK HRELREDSIR SHFSVA
Length:536
Mass (Da):59,808
Last modified:October 3, 2012 - v3
Checksum:i78E14B5C4711411E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41L → F in BAA11241 (PubMed:8620881).Curated
Sequence conflicti4 – 41L → F in AAF00104 (PubMed:10468599).Curated
Sequence conflicti4 – 41L → F in AAF00105 (PubMed:10468599).Curated
Sequence conflicti4 – 41L → F in AAF00107 (PubMed:10468599).Curated
Sequence conflicti58 – 592DS → IT in BAA11241 (PubMed:8620881).Curated
Sequence conflicti132 – 1321E → G in BAA11241 (PubMed:8620881).Curated
Sequence conflicti293 – 2931G → V in AAB51036 (PubMed:9032278).Curated
Sequence conflicti348 – 3481Y → C in AAB51036 (PubMed:9032278).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti66 – 7712Missing in Lgj-2J. 1 Publication
Add
BLAST
Natural varianti168 – 1681H → N in Lgj. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78175 mRNA. Translation: BAA11241.1.
AF131861 Genomic DNA. Translation: AAF00104.1.
AF131862 Genomic DNA. Translation: AAF00105.1.
AF131864 Genomic DNA. Translation: AAF00107.1.
AC134793 Genomic DNA. No translation available.
CH466581 Genomic DNA. Translation: EDL03281.1.
U72676 mRNA. Translation: AAB51036.1.
CCDSiCCDS27386.1.
PIRiS71332.
RefSeqiNP_032754.2. NM_008728.2.
UniGeneiMm.25259.

Genome annotation databases

EnsembliENSMUST00000066529; ENSMUSP00000066737; ENSMUSG00000022206.
GeneIDi18162.
KEGGimmu:18162.
UCSCiuc007vhf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78175 mRNA. Translation: BAA11241.1.
AF131861 Genomic DNA. Translation: AAF00104.1.
AF131862 Genomic DNA. Translation: AAF00105.1.
AF131864 Genomic DNA. Translation: AAF00107.1.
AC134793 Genomic DNA. No translation available.
CH466581 Genomic DNA. Translation: EDL03281.1.
U72676 mRNA. Translation: AAB51036.1.
CCDSiCCDS27386.1.
PIRiS71332.
RefSeqiNP_032754.2. NM_008728.2.
UniGeneiMm.25259.

3D structure databases

ProteinModelPortaliP70180.
SMRiP70180. Positions 41-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46248N.
IntActiP70180. 1 interaction.
MINTiMINT-4087811.
STRINGi10090.ENSMUSP00000066737.

Chemistry

BindingDBiP70180.
ChEMBLiCHEMBL4711.

PTM databases

PhosphoSiteiP70180.

Proteomic databases

MaxQBiP70180.
PaxDbiP70180.
PRIDEiP70180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066529; ENSMUSP00000066737; ENSMUSG00000022206.
GeneIDi18162.
KEGGimmu:18162.
UCSCiuc007vhf.2. mouse.

Organism-specific databases

CTDi4883.
MGIiMGI:97373. Npr3.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00440000033872.
HOGENOMiHOG000236252.
HOVERGENiHBG060145.
InParanoidiP70180.
KOiK12325.
OMAiFQVAYED.
OrthoDBiEOG7BZVS0.
TreeFamiTF106339.

Miscellaneous databases

PROiP70180.
SOURCEiSearch...

Gene expression databases

CleanExiMM_NPR3.
GenevisibleiP70180. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the 5'-flanking regulatory region of the mouse gene encoding the clearance receptor for atrial natriuretic peptide."
    Yanaka N., Kotera J., Taguchi I., Sugiura M., Kawashima K., Omori K.
    Eur. J. Biochem. 237:25-34(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Lung.
  2. "Three new allelic mouse mutations that cause skeletal overgrowth involve the natriuretic peptide receptor C gene (Npr3)."
    Jaubert J., Jaubert F., Martin N., Washburn L.L., Lee B.K., Eicher E.M., Guenet J.-L.
    Proc. Natl. Acad. Sci. U.S.A. 96:10278-10283(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LGJ ASN-168, VARIANT LGJ-2J 66-ARG--SER-77 DEL, POSSIBLE INVOLVEMENT IN SKELETAL-OVERGROWTH SYNDROME.
    Strain: 129/Sv and BALB/c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts."
    Fu X., Kamps M.P.
    Mol. Cell. Biol. 17:1503-1512(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 255-439.
  6. "The natriuretic peptide clearance receptor locally modulates the physiological effects of the natriuretic peptide system."
    Matsukawa N., Grzesik W.J., Takahashi N., Pandey K.N., Pang S., Yamauchi M., Smithies O.
    Proc. Natl. Acad. Sci. U.S.A. 96:7403-7408(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart and Lung.

Entry informationi

Entry nameiANPRC_MOUSE
AccessioniPrimary (citable) accession number: P70180
Secondary accession number(s): G3X9E5
, P97804, Q9R025, Q9R027, Q9R028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2012
Last modified: June 8, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has low affinity for peptide hormones in the absence of bound chloride.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.