Reviewed, UniProtKB/Swiss-Prot P70174 (HRH1_MOUSE)

Names and origin

Protein names

Recommended name:
    Histamine H1 receptor

Gene names

Name:	Hrh1
Synonyms:	Bphs

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Involved in circadian rhythm of locomotor activity and exploratory behavior. Also involved in responsiveness to pertussis toxin through its control of susceptibility to histamine hypersensitivity and enhancement of antigen-specific delayed-type hypersensitivity responses. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule.
Polymorphism	Strains C3H/HeJ and CBA/J are resistant to vasoactive amine sensitization elicited by histamine (VAASH) which is induced by pertussis toxin.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Biological rhythms
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histamine receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Histamine H1 receptor		PRO_0000069677
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 49	20	1 Potential
	Topological domain	50 – 63	14	Cytoplasmic Potential
	Transmembrane	64 – 83	20	2 Potential
	Topological domain	84 – 101	18	Extracellular Potential
	Transmembrane	102 – 123	22	3 Potential
	Topological domain	124 – 145	22	Cytoplasmic Potential
	Transmembrane	146 – 165	20	4 Potential
	Topological domain	166 – 189	24	Extracellular Potential
	Transmembrane	190 – 210	21	5 Potential
	Topological domain	211 – 419	209	Cytoplasmic Potential
	Transmembrane	420 – 439	20	6 Potential
	Topological domain	440 – 451	12	Extracellular Potential
	Transmembrane	452 – 471	20	7 Potential
	Topological domain	472 – 488	17	Cytoplasmic Potential
Amino acid modifications
	Modified residue	255	1	Phosphoserine By similarity
	Modified residue	275	1	Phosphoserine By similarity
	Lipidation	446	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Disulfide bond	100 ↔ 180		By similarity
Natural variations
	Natural variant	263	1	P → L in strain: C3H/HeJ and CBA/J. Ref.2
	Natural variant	312	1	V → M in strain: C3H/HeJ and CBA/J. Ref.2
	Natural variant	330	1	P → S in strain: C3H/HeJ and CBA/J. Ref.2
Experimental info
	Sequence conflict	2	1	S → R in BAA08791. Ref.1
	Sequence conflict	47	1	L → G in BAA08791. Ref.1
	Sequence conflict	79	1	V → I in BAA08791. Ref.1
	Sequence conflict	215 – 216	2	KA → NG in BAA08791. Ref.1
	Sequence conflict	224	1	R → P in BAA08791. Ref.1
	Sequence conflict	242	1	E → D in AAK66778. Ref.2
	Sequence conflict	244	1	A → S in BAA08791. Ref.1
	Sequence conflict	261	1	K → T in BAA08791. Ref.1
	Sequence conflict	278	1	E → A in BAA08791. Ref.1
	Sequence conflict	379	1	S → R in BAA08791. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P70174-1 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 82612DF712998B41	FASTA	488	55,682
	10 20 30 40 50 60 MSLPNTSSAS EDKMCEGNRT AMASPQLLPL VVVLSSISLV TVGLNLLVLY AVRSERKLHT 70 80 90 100 110 120 VGNLYIVSLS VADLIVGAVV MPMNILYLIM TKWSLGRPLC LFWLSMDYVA STASIFSVFI 130 140 150 160 170 180 LCIDRYRSVQ QPLRYLRYRT KTRASATILG AWFLSFLWVI PILGWHHFTP LAPELREDKC 190 200 210 220 230 240 ETDFYNVTWF KIMTAIINFY LPTLLMLWFY VKIYKAVRRH CQHRQLTNGS LPTFLEIKLR 250 260 270 280 290 300 SEDAKEGAKK PGKESPWGVQ KRPSRDPTGG LDQKSTSEDP KVTSPTVFSQ EGERETVTRP 310 320 330 340 350 360 CFRLDVMQTQ PVPEGDARGS KANDQTLSQP KMDEQSLSTC RRISETSEDQ TLVDRQSFSR 370 380 390 400 410 420 TTDSDTSIEP GLGKVKARSR SNSGLDYIKV TWKRLRSHSR QYVSGLHLNR ERKAAKQLGC 430 440 450 460 470 480 IMAAFILCWI PYFIFFMVIA FCNSCCSEPV HMFTIWLGYI NSTLNPLIYP LCNENFKKTF KKILHIRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characteristics of the mouse genomic histamine H1 receptor gene." Inoue I., Taniuchi I., Kitamura D., Jenkins N.A., Gilbert D.J., Copeland N.G., Watanabe T. Genomics 36:178-181(1996) [PubMed: 8812432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
[2]	"Identification of Bphs, an autoimmune disease locus, as histamine receptor H1." Ma R.Z., Gao J., Meeker N.D., Fillmore P.D., Tung K.S.K., Watanabe T., Zachary J.F., Offner H., Blankenhorn E.P., Teuscher C. Science 297:620-623(2002) [PubMed: 12142541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, VARIANTS LEU-263; MET-312 AND SER-330. Strain: 129/SvJ, A/J, B10.S/DvTe, BALB/cByJ, BALB/cJ, C3H/HeJ, C57BL/6J, CBA/J, DBA/1, DBA/2, FVB/NCr, NOD, SJL and SWR. Tissue: Spleen.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Adipose tissue, Cerebellum, Hypothalamus and Mesonephros.
[4]	"Impaired locomotor activity and exploratory behavior in mice lacking histamine H1 receptors." Inoue I., Yanai K., Kitamura D., Taniuchi I., Kobayashi T., Niimura K., Watanabe T., Watanabe T. Proc. Natl. Acad. Sci. U.S.A. 93:13316-13320(1996) [PubMed: 8917588] [Abstract] Cited for: FUNCTION.
[5]	"Analysis of the role of Bphs/Hrh1 in the genetic control of responsiveness to pertussis toxin." Gao J.F., Call S.B., Fillmore P.D., Watanabe T., Meeker N.D., Teuscher C. Infect. Immun. 71:1281-1287(2003) [PubMed: 12595443] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50095 Genomic DNA. Translation: BAA08791.1. AF387890 mRNA. Translation: AAK71654.1. AF387891 mRNA. Translation: AAK71655.1. AF387892 mRNA. Translation: AAK71656.1. AF387893 mRNA. Translation: AAK71657.1. AF387894 mRNA. Translation: AAK71658.1. AF387895 mRNA. Translation: AAK71659.1. AF387896 mRNA. Translation: AAK71660.1. AF388052 mRNA. Translation: AAK66774.2. AF388053 mRNA. Translation: AAK66775.1. AF388054 mRNA. Translation: AAK66776.1. AF388055 mRNA. Translation: AAK66777.1. AF388056 mRNA. Translation: AAK66778.1. AF388057 mRNA. Translation: AAK66779.1. AF388058 mRNA. Translation: AAK66780.1. AK032763 mRNA. Translation: BAC28011.1. AK038480 mRNA. Translation: BAC30013.1. AK046607 mRNA. Translation: BAC32805.1. AK047070 mRNA. Translation: BAC32950.1.
IPI	IPI00136348.
RefSeq	NP_032311.2.
UniGene	Mm.333327
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P70174.
Proteomic databases
PRIDE	P70174.
Genome annotation databases
Ensembl	ENSMUSG00000053004. Mus musculus. [Contig view]
GeneID	15465.
KEGG	mmu:15465.
Organism-specific databases
MGI	MGI:107619. Hrh1.
Phylogenomic databases
HOGENOM	P70174.
HOVERGEN	P70174.
OMA	P70174. LHMFTIW.
Gene expression databases
ArrayExpress	P70174.
Bgee	P70174.
CleanEx	MM_HRH1.
GermOnline	ENSMUSG00000053004. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000921. Histamine_H1_recept. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00530. HISTAMINEH1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288290.
SOURCE	Search...

Entry information

Entry name

HRH1_MOUSE

Accession

Primary (citable) accession number: P70174
Secondary accession number(s): Q91V75 , Q91XN0, Q91XN1, Q91XN2, Q91XN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 9, 2003
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)