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Protein

Ileal sodium/bile acid cotransporter

Gene

Slc10a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism (By similarity).By similarity

GO - Molecular functioni

  1. bile acid:sodium symporter activity Source: GO_Central

GO - Biological processi

  1. bile acid and bile salt transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_297274. Orphan transporters.
REACT_300927. Recycling of bile acids and salts.

Names & Taxonomyi

Protein namesi
Recommended name:
Ileal sodium/bile acid cotransporter
Alternative name(s):
Apical sodium-dependent bile acid transporter
Short name:
ASBT
Ileal Na(+)/bile acid cotransporter
Ileal sodium-dependent bile acid transporter
Short name:
IBAT
Short name:
ISBT
Na(+)-dependent ileal bile acid transporter
Sodium/taurocholate cotransporting polypeptide, ileal
Solute carrier family 10 member 2
Gene namesi
Name:Slc10a2
Synonyms:Ntcp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1201406. Slc10a2.

Subcellular locationi

  1. Membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828ExtracellularSequence AnalysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Topological domaini50 – 8738CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 12618ExtracellularSequence AnalysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence AnalysisAdd
BLAST
Topological domaini148 – 15710CytoplasmicSequence Analysis
Transmembranei158 – 17821HelicalSequence AnalysisAdd
BLAST
Topological domaini179 – 19517ExtracellularSequence AnalysisAdd
BLAST
Transmembranei196 – 21621HelicalSequence AnalysisAdd
BLAST
Topological domaini217 – 2248CytoplasmicSequence Analysis
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST
Topological domaini246 – 28439ExtracellularSequence AnalysisAdd
BLAST
Transmembranei285 – 30521HelicalSequence AnalysisAdd
BLAST
Topological domaini306 – 34843CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. integral component of plasma membrane Source: GO_Central
  3. microvillus Source: MGI
  4. nucleus Source: Ensembl
  5. proteasome complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Ileal sodium/bile acid cotransporterPRO_0000052340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP70172.

PTM databases

PhosphoSiteiP70172.

Expressioni

Gene expression databases

BgeeiP70172.
CleanExiMM_SLC10A2.
ExpressionAtlasiP70172. baseline and differential.
GenevestigatoriP70172.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP70172.
SMRiP70172. Positions 41-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0385.
HOGENOMiHOG000234524.
HOVERGENiHBG006537.
InParanoidiP70172.
KOiK14342.
OMAiNILAYWI.
OrthoDBiEOG7XWPNW.
PhylomeDBiP70172.
TreeFamiTF315811.

Family and domain databases

InterProiIPR002657. BilAc:Na_symport/Acr3.
IPR004710. Bilac:Na_transpt.
IPR030207. SLC10A2.
[Graphical view]
PANTHERiPTHR10361. PTHR10361. 1 hit.
PTHR10361:SF19. PTHR10361:SF19. 1 hit.
PfamiPF01758. SBF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00841. bass. 1 hit.

Sequencei

Sequence statusi: Complete.

P70172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNSSVCPPN ATVCEGDSCV VPESNFNAIL NTVMSTVLTI LLAMVMFSMG
60 70 80 90 100
CNVEVHKFLG HIKRPWGIFV GFLCQFGIMP LTGFILSVAS GILPVQAVVV
110 120 130 140 150
LIMGCCPGGT GSNILAYWID GDMDLSVSMT TCSTLLALGM MPLCLFVYTK
160 170 180 190 200
MWVDSGTIVI PYDSIGISLV ALVIPVSFGM FVNHKWPQKA KIILKIGSIT
210 220 230 240 250
GVILIVLIAV IGGILYQSAW IIEPKLWIIG TIFPIAGYSL GFFLARLAGQ
260 270 280 290 300
PWYRCRTVAL ETGMQNTQLC STIVQLSFSP EDLNLVFTFP LIYTVFQLVF
310 320 330 340
AAVILGIYVT YRKCYGKNDA EFLEKTDNEM DSRPSFDETN KGFQPDEK
Length:348
Mass (Da):38,134
Last modified:January 1, 1998 - v2
Checksum:iD00B5E43431875D7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291I → V in strain: NZB/BlNJ and C57BL/6. 2 Publications
Natural varianti36 – 361T → S in strain: NZB/BlNJ and C57BL/6. 2 Publications
Natural varianti40 – 401I → T in strain: NZB/BlNJ and C57BL/6. 2 Publications
Natural varianti178 – 1781F → I in strain: NZB/BlNJ and C57BL/6. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002693 mRNA. Translation: BAA19606.1.
D87059 mRNA. Translation: BAA13237.1.
AF271073
, AF266724, AF266725, AF266726, AF266727, AF266728 Genomic DNA. Translation: AAK55514.1.
AY529655 mRNA. Translation: AAS20611.1.
AB078635 Genomic DNA. Translation: BAB84081.1.
CCDSiCCDS22089.1.
RefSeqiNP_035518.1. NM_011388.2.
UniGeneiMm.3500.

Genome annotation databases

EnsembliENSMUST00000023835; ENSMUSP00000023835; ENSMUSG00000023073.
GeneIDi20494.
KEGGimmu:20494.
UCSCiuc009kub.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002693 mRNA. Translation: BAA19606.1.
D87059 mRNA. Translation: BAA13237.1.
AF271073
, AF266724, AF266725, AF266726, AF266727, AF266728 Genomic DNA. Translation: AAK55514.1.
AY529655 mRNA. Translation: AAS20611.1.
AB078635 Genomic DNA. Translation: BAB84081.1.
CCDSiCCDS22089.1.
RefSeqiNP_035518.1. NM_011388.2.
UniGeneiMm.3500.

3D structure databases

ProteinModelPortaliP70172.
SMRiP70172. Positions 41-267.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP70172.
ChEMBLiCHEMBL2073708.

PTM databases

PhosphoSiteiP70172.

Proteomic databases

PRIDEiP70172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023835; ENSMUSP00000023835; ENSMUSG00000023073.
GeneIDi20494.
KEGGimmu:20494.
UCSCiuc009kub.1. mouse.

Organism-specific databases

CTDi6555.
MGIiMGI:1201406. Slc10a2.

Phylogenomic databases

eggNOGiCOG0385.
HOGENOMiHOG000234524.
HOVERGENiHBG006537.
InParanoidiP70172.
KOiK14342.
OMAiNILAYWI.
OrthoDBiEOG7XWPNW.
PhylomeDBiP70172.
TreeFamiTF315811.

Enzyme and pathway databases

ReactomeiREACT_297274. Orphan transporters.
REACT_300927. Recycling of bile acids and salts.

Miscellaneous databases

NextBioi298641.
PROiP70172.
SOURCEiSearch...

Gene expression databases

BgeeiP70172.
CleanExiMM_SLC10A2.
ExpressionAtlasiP70172. baseline and differential.
GenevestigatoriP70172.

Family and domain databases

InterProiIPR002657. BilAc:Na_symport/Acr3.
IPR004710. Bilac:Na_transpt.
IPR030207. SLC10A2.
[Graphical view]
PANTHERiPTHR10361. PTHR10361. 1 hit.
PTHR10361:SF19. PTHR10361:SF19. 1 hit.
PfamiPF01758. SBF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00841. bass. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter."
    Saeki T., Matoba K., Furukawa H., Kirifuji K., Kanamoto R., Iwami K.
    J. Biochem. 125:846-851(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. "Disruption of the ileal bile acid transporter gene in mice."
    Dawson P.A., Craddock A.L., Tietjen M.E., Haywood J.H.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE, VARIANTS VAL-29; SER-36; THR-40 AND ILE-178.
    Strain: C57BL/6.
  3. "New genetic determinants of cholesterol gallstone formation revealed in an intercross of mouse strains NZB and SM."
    Lyons M.A., Korstanje R., Li R., Sheehan S.M., Walsh K.A., Carey M.C., Churchill G.A., Paigen B.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE, VARIANTS VAL-29; SER-36; THR-40 AND ILE-178.
    Strain: NZB/BlNJ and SM/J.
  4. "Identification of transcription start sites in mouse ileal sodium-dependent bile acid transporter gene."
    Saeki T., Kirifuji K., Kanamoto R., Iwami K.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 1-125.

Entry informationi

Entry nameiNTCP2_MOUSE
AccessioniPrimary (citable) accession number: P70172
Secondary accession number(s): Q8VI83, Q925U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 1, 1998
Last modified: April 1, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.