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P70168 (IMB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Importin subunit beta-1
Alternative name(s):
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name=PTAC97
SCG
Gene names
Name:Kpnb1
Synonyms:Impnb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus By similarity. Ref.6

Subunit structure

Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones By similarity. Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota By similarity. Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) By similarity. Ref.6 Ref.7 Ref.9

Subcellular location

Cytoplasm By similarity. Nucleus envelope By similarity.

Post-translational modification

Mono-ADP-ribosylated by PARP16 By similarity.

Sequence similarities

Belongs to the importin beta family.

Contains 8 HEAT repeats.

Contains 1 importin N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Importin subunit beta-1
PRO_0000120746

Regions

Domain21 – 10181Importin N-terminal
Repeat124 – 16340HEAT 1
Repeat168 – 20740HEAT 2
Repeat213 – 25038HEAT 3
Repeat317 – 35741HEAT 4
Repeat362 – 39938HEAT 5
Repeat404 – 44138HEAT 6
Repeat602 – 64140HEAT 7
Repeat687 – 72640HEAT 8
Region329 – 34214IAB-binding
Region334 – 41986Ran-GTP binding By similarity
Compositional bias337 – 3415Poly-Asp

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue121Phosphoserine By similarity
Modified residue2111N6-acetyllysine; alternate By similarity
Modified residue8351N6-acetyllysine By similarity
Modified residue8671N6-acetyllysine By similarity
Cross-link206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity

Experimental info

Sequence conflict3881M → V in BAA11034. Ref.1

Secondary structure

.................................................................................................................... 876
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P70168 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 00D6B15023A30598

FASTA87697,184
        10         20         30         40         50         60 
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS QVARVAAGLQ 

        70         80         90        100        110        120 
IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG TETYRPSSAS QCVAGIACAE 

       130        140        150        160        170        180 
IPVSQWPELI PQLVANVTNP NSTEHMKEST LEAIGYICQD IDPEQLQDKS NEILTAIIQG 

       190        200        210        220        230        240 
MRKEEPSNNV KLAATNALLN SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN 

       250        260        270        280        290        300 
LVKIMSLYYQ YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA 

       310        320        330        340        350        360 
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV CLMLLSTCCE 

       370        380        390        400        410        420 
DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN QLKPLVIQAM PTLIELMKDP 

       430        440        450        460        470        480 
SVVVRDTTAW TVGRICELLP EAAINDVYLA PLLQCLIEGL SAEPRVASNV CWAFSSLAEA 

       490        500        510        520        530        540 
AYEAADVADD QEEPATYCLS SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA 

       550        560        570        580        590        600 
KDCYPAVQKT TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA 

       610        620        630        640        650        660 
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF KPFLGIGLKN 

       670        680        690        700        710        720 
YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL GNENVHRSVK PQILSVFGDI 

       730        740        750        760        770        780 
ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ 

       790        800        810        820        830        840 
ENVHPDVMLV QPRVEFILSF IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR 

       850        860        870 
PMIHELLTEG RRSKTNKAKT LATWATKELR KLKNQA 

« Hide

References

« Hide 'large scale' references
[1]"Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1."
Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.
Genomics 36:213-215(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile."
Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.
FEBS Lett. 368:415-419(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92; 212-221; 377-389 AND 860-867.
Strain: C57BL/6.
Tissue: Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Limb.
[6]"Multiple pathways contribute to nuclear import of core histones."
Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
[7]"The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1."
Forwood J.K., Harley V., Jans D.A.
J. Biol. Chem. 276:46575-46582(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRY.
[8]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[9]"Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse."
Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.
J. Biol. Chem. 285:33113-33122(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KPNA7.
Strain: C57BL/6.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D67015 mRNA. Translation: BAA11034.1.
D45836 mRNA. Translation: BAA08273.1.
AL627445 Genomic DNA. Translation: CAM26994.1.
CH466556 Genomic DNA. Translation: EDL16074.1.
BC052438 mRNA. Translation: AAH52438.1.
BC055115 mRNA. Translation: AAH55115.1.
CCDSCCDS25316.1.
PIRS66288.
RefSeqNP_032405.3. NM_008379.3.
UniGeneMm.251013.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCJX-ray2.60A/B1-449[»]
1UKLX-ray3.00A/B1-876[»]
ProteinModelPortalP70168.
SMRP70168. Positions 1-876.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200654. 8 interactions.
DIPDIP-33404N.
IntActP70168. 13 interactions.
MINTMINT-6478527.

PTM databases

PhosphoSiteP70168.

Proteomic databases

MaxQBP70168.
PaxDbP70168.
PRIDEP70168.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
GeneID16211.
KEGGmmu:16211.
UCSCuc007ldu.1. mouse.

Organism-specific databases

CTD3837.
MGIMGI:107532. Kpnb1.

Phylogenomic databases

eggNOGCOG5215.
GeneTreeENSGT00550000074898.
HOGENOMHOG000204108.
HOVERGENHBG002369.
InParanoidQ6GTI5.
KOK14293.
OMAGLTIMGM.
OrthoDBEOG7GN2KZ.
TreeFamTF105655.

Gene expression databases

ArrayExpressP70168.
BgeeP70168.
CleanExMM_KPNB1.
GenevestigatorP70168.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view]
PANTHERPTHR10527:SF1. PTHR10527:SF1. 1 hit.
PfamPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKPNB1. mouse.
EvolutionaryTraceP70168.
NextBio289169.
PROP70168.
SOURCESearch...

Entry information

Entry nameIMB1_MOUSE
AccessionPrimary (citable) accession number: P70168
Secondary accession number(s): Q62117, Q6GTI5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot