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Protein

Importin subunit beta-1

Gene

Kpnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • protein import into nucleus Source: MGI
  • ribosomal protein import into nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_276462. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_299111. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1
Alternative name(s):
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name:
PTAC97
SCG
Gene namesi
Name:Kpnb1
Synonyms:Impnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107532. Kpnb1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Importin subunit beta-1PRO_0000120746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei211 – 2111N6-acetyllysine; alternateBy similarity
Cross-linki211 – 211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei835 – 8351N6-acetyllysineBy similarity
Modified residuei867 – 8671N6-acetyllysineBy similarity

Post-translational modificationi

Mono-ADP-ribosylated by PARP16.By similarity

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP70168.
PaxDbiP70168.
PRIDEiP70168.

PTM databases

PhosphoSiteiP70168.

Expressioni

Gene expression databases

BgeeiP70168.
CleanExiMM_KPNB1.
ExpressionAtlasiP70168. baseline and differential.
GenevisibleiP70168. MM.

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota (By similarity). Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) (By similarity). Interacts with SLC35G1 and STIM1 (By similarity). Interacts with DCAF8. Interacts with RAN (PubMed:25946333).By similarity1 Publication

Protein-protein interaction databases

BioGridi200654. 8 interactions.
DIPiDIP-33404N.
IntActiP70168. 14 interactions.
MINTiMINT-6478527.
STRINGi10090.ENSMUSP00000001479.

Structurei

Secondary structure

876
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 77Combined sources
Turni8 – 114Combined sources
Helixi15 – 4531Combined sources
Beta strandi47 – 493Combined sources
Helixi51 – 6212Combined sources
Turni63 – 653Combined sources
Helixi70 – 8213Combined sources
Helixi85 – 9814Combined sources
Beta strandi104 – 1063Combined sources
Helixi108 – 12013Combined sources
Helixi121 – 1233Combined sources
Helixi129 – 13810Combined sources
Helixi144 – 16017Combined sources
Helixi163 – 1664Combined sources
Helixi167 – 1693Combined sources
Helixi170 – 18112Combined sources
Helixi188 – 20114Combined sources
Turni202 – 2043Combined sources
Helixi206 – 2094Combined sources
Helixi212 – 22514Combined sources
Helixi231 – 24717Combined sources
Helixi249 – 2546Combined sources
Turni255 – 2595Combined sources
Helixi260 – 2689Combined sources
Helixi273 – 30230Combined sources
Helixi314 – 32916Combined sources
Helixi330 – 3323Combined sources
Beta strandi338 – 3403Combined sources
Helixi344 – 35916Combined sources
Helixi360 – 3623Combined sources
Helixi363 – 37412Combined sources
Helixi380 – 39213Combined sources
Beta strandi394 – 3974Combined sources
Helixi399 – 41618Combined sources
Helixi422 – 43817Combined sources
Helixi440 – 4445Combined sources
Beta strandi447 – 4493Combined sources
Helixi464 – 48522Combined sources
Helixi486 – 4883Combined sources
Beta strandi491 – 4955Combined sources
Helixi500 – 51415Combined sources
Turni517 – 5204Combined sources
Helixi521 – 5233Combined sources
Helixi524 – 53714Combined sources
Helixi544 – 56320Combined sources
Helixi564 – 5674Combined sources
Helixi571 – 59222Combined sources
Helixi597 – 61620Combined sources
Helixi625 – 63915Combined sources
Turni640 – 6434Combined sources
Helixi644 – 6485Combined sources
Helixi651 – 6599Combined sources
Helixi661 – 6633Combined sources
Helixi664 – 68017Combined sources
Helixi682 – 6854Combined sources
Helixi686 – 70116Combined sources
Beta strandi703 – 7053Combined sources
Helixi709 – 72416Combined sources
Helixi725 – 7284Combined sources
Helixi729 – 74315Combined sources
Helixi753 – 77725Combined sources
Beta strandi779 – 7824Combined sources
Helixi785 – 7906Combined sources
Helixi793 – 80614Combined sources
Helixi812 – 82817Combined sources
Helixi834 – 8385Combined sources
Helixi841 – 8499Combined sources
Helixi856 – 87116Combined sources
Helixi872 – 8754Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCJX-ray2.60A/B1-449[»]
1UKLX-ray3.00A/B1-876[»]
ProteinModelPortaliP70168.
SMRiP70168. Positions 1-876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70168.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati3 – 2927HEAT 11 PublicationAdd
BLAST
Domaini21 – 10181Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati32 – 6231HEAT 21 PublicationAdd
BLAST
Repeati85 – 12036HEAT 31 PublicationAdd
BLAST
Repeati129 – 16032HEAT 41 PublicationAdd
BLAST
Repeati170 – 20132HEAT 51 PublicationAdd
BLAST
Repeati212 – 24736HEAT 61 PublicationAdd
BLAST
Repeati260 – 30243HEAT 71 PublicationAdd
BLAST
Repeati314 – 35946HEAT 81 PublicationAdd
BLAST
Repeati363 – 39230HEAT 91 PublicationAdd
BLAST
Repeati399 – 43840HEAT 101 PublicationAdd
BLAST
Repeati449 – 48537HEAT 111 PublicationAdd
BLAST
Repeati500 – 53738HEAT 121 PublicationAdd
BLAST
Repeati544 – 59249HEAT 131 PublicationAdd
BLAST
Repeati597 – 63943HEAT 141 PublicationAdd
BLAST
Repeati644 – 68037HEAT 151 PublicationAdd
BLAST
Repeati686 – 72439HEAT 161 PublicationAdd
BLAST
Repeati729 – 77749HEAT 171 PublicationAdd
BLAST
Repeati785 – 82844HEAT 181 PublicationAdd
BLAST
Repeati834 – 87542HEAT 191 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 34214IAB-bindingAdd
BLAST
Regioni334 – 41986Ran-GTP bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3415Poly-Asp

Sequence similaritiesi

Contains 19 HEAT repeats.1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiP70168.
KOiK14293.
OMAiMSNILPY.
OrthoDBiEOG7GN2KZ.
TreeFamiTF105655.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view]
PANTHERiPTHR10527:SF1. PTHR10527:SF1. 1 hit.
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS
60 70 80 90 100
QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG
110 120 130 140 150
TETYRPSSAS QCVAGIACAE IPVSQWPELI PQLVANVTNP NSTEHMKEST
160 170 180 190 200
LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN
210 220 230 240 250
SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ
260 270 280 290 300
YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA
310 320 330 340 350
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV
360 370 380 390 400
CLMLLSTCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN
410 420 430 440 450
QLKPLVIQAM PTLIELMKDP SVVVRDTTAW TVGRICELLP EAAINDVYLA
460 470 480 490 500
PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS
510 520 530 540 550
SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT
560 570 580 590 600
TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA
610 620 630 640 650
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF
660 670 680 690 700
KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL
710 720 730 740 750
GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS
760 770 780 790 800
DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF
810 820 830 840 850
IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG
860 870
RRSKTNKAKT LATWATKELR KLKNQA
Length:876
Mass (Da):97,184
Last modified:July 27, 2011 - v2
Checksum:i00D6B15023A30598
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3881M → V in BAA11034 (PubMed:8812441).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67015 mRNA. Translation: BAA11034.1.
D45836 mRNA. Translation: BAA08273.1.
AL627445 Genomic DNA. Translation: CAM26994.1.
CH466556 Genomic DNA. Translation: EDL16074.1.
BC052438 mRNA. Translation: AAH52438.1.
BC055115 mRNA. Translation: AAH55115.1.
CCDSiCCDS25316.1.
PIRiS66288.
RefSeqiNP_032405.3. NM_008379.3.
UniGeneiMm.251013.

Genome annotation databases

EnsembliENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
GeneIDi16211.
KEGGimmu:16211.
UCSCiuc007ldu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67015 mRNA. Translation: BAA11034.1.
D45836 mRNA. Translation: BAA08273.1.
AL627445 Genomic DNA. Translation: CAM26994.1.
CH466556 Genomic DNA. Translation: EDL16074.1.
BC052438 mRNA. Translation: AAH52438.1.
BC055115 mRNA. Translation: AAH55115.1.
CCDSiCCDS25316.1.
PIRiS66288.
RefSeqiNP_032405.3. NM_008379.3.
UniGeneiMm.251013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCJX-ray2.60A/B1-449[»]
1UKLX-ray3.00A/B1-876[»]
ProteinModelPortaliP70168.
SMRiP70168. Positions 1-876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200654. 8 interactions.
DIPiDIP-33404N.
IntActiP70168. 14 interactions.
MINTiMINT-6478527.
STRINGi10090.ENSMUSP00000001479.

PTM databases

PhosphoSiteiP70168.

Proteomic databases

MaxQBiP70168.
PaxDbiP70168.
PRIDEiP70168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
GeneIDi16211.
KEGGimmu:16211.
UCSCiuc007ldu.1. mouse.

Organism-specific databases

CTDi3837.
MGIiMGI:107532. Kpnb1.

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiP70168.
KOiK14293.
OMAiMSNILPY.
OrthoDBiEOG7GN2KZ.
TreeFamiTF105655.

Enzyme and pathway databases

ReactomeiREACT_276462. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_299111. Activation of DNA fragmentation factor.

Miscellaneous databases

ChiTaRSiKpnb1. mouse.
EvolutionaryTraceiP70168.
NextBioi289169.
PROiP70168.
SOURCEiSearch...

Gene expression databases

BgeeiP70168.
CleanExiMM_KPNB1.
ExpressionAtlasiP70168. baseline and differential.
GenevisibleiP70168. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view]
PANTHERiPTHR10527:SF1. PTHR10527:SF1. 1 hit.
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1."
    Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.
    Genomics 36:213-215(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile."
    Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.
    FEBS Lett. 368:415-419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92; 212-221; 377-389 AND 860-867.
    Strain: C57BL/6.
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Limb.
  6. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  7. "The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1."
    Forwood J.K., Harley V., Jans D.A.
    J. Biol. Chem. 276:46575-46582(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRY.
  8. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. "Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse."
    Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.
    J. Biol. Chem. 285:33113-33122(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KPNA7.
    Strain: C57BL/6.
    Tissue: Ovary.
  10. "The inner nuclear membrane protein Nemp1 is a new type of RanGTP-binding protein in eukaryotes."
    Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M.
    PLoS ONE 10:E0127271-E0127271(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAN.
  11. "The adoption of a twisted structure of importin-beta is essential for the protein-protein interaction required for nuclear transport."
    Lee S.J., Imamoto N., Sakai H., Nakagawa A., Kose S., Koike M., Yamamoto M., Kumasaka T., Yoneda Y., Tsukihara T.
    J. Mol. Biol. 302:251-264(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 1-449, REPEAT STRUCTURE.
  12. "The structure of importin-beta bound to SREBP-2: nuclear import of a transcription factor."
    Lee S.J., Sekimoto T., Yamashita E., Nagoshi E., Nakagawa A., Imamoto N., Yoshimura M., Sakai H., Chong K.T., Tsukihara T., Yoneda Y.
    Science 302:1571-1575(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS), REPEAT STRUCTURE.

Entry informationi

Entry nameiIMB1_MOUSE
AccessioniPrimary (citable) accession number: P70168
Secondary accession number(s): Q62117, Q6GTI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.