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P70168

- IMB1_MOUSE

UniProt

P70168 - IMB1_MOUSE

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Protein
Importin subunit beta-1
Gene
Kpnb1, Impnb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus By similarity.1 Publication

GO - Molecular functioni

  1. protein binding Source: MGI
  2. protein transporter activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. protein import into nucleus Source: MGI
  2. ribosomal protein import into nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_205002. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1
Alternative name(s):
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name:
PTAC97
SCG
Gene namesi
Name:Kpnb1
Synonyms:Impnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:107532. Kpnb1.

Subcellular locationi

Cytoplasm By similarity. Nucleus envelope By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nuclear membrane Source: Ensembl
  3. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Importin subunit beta-1
PRO_0000120746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei12 – 121Phosphoserine By similarity
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei211 – 2111N6-acetyllysine; alternate By similarity
Cross-linki211 – 211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate By similarity
Modified residuei835 – 8351N6-acetyllysine By similarity
Modified residuei867 – 8671N6-acetyllysine By similarity

Post-translational modificationi

Mono-ADP-ribosylated by PARP16 By similarity.

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP70168.
PaxDbiP70168.
PRIDEiP70168.

PTM databases

PhosphoSiteiP70168.

Expressioni

Gene expression databases

ArrayExpressiP70168.
BgeeiP70168.
CleanExiMM_KPNB1.
GenevestigatoriP70168.

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones By similarity. Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota By similarity. Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) By similarity.3 Publications

Protein-protein interaction databases

BioGridi200654. 8 interactions.
DIPiDIP-33404N.
IntActiP70168. 13 interactions.
MINTiMINT-6478527.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 77
Turni8 – 114
Helixi15 – 4531
Beta strandi47 – 493
Helixi51 – 6212
Turni63 – 653
Helixi70 – 8213
Helixi85 – 9814
Beta strandi104 – 1063
Helixi108 – 12013
Helixi121 – 1233
Helixi129 – 13810
Helixi144 – 16017
Helixi163 – 1664
Helixi167 – 1693
Helixi170 – 18112
Helixi188 – 20114
Turni202 – 2043
Helixi206 – 2094
Helixi212 – 22514
Helixi231 – 24717
Helixi249 – 2546
Turni255 – 2595
Helixi260 – 2689
Helixi273 – 30230
Helixi314 – 32916
Helixi330 – 3323
Beta strandi338 – 3403
Helixi344 – 35916
Helixi360 – 3623
Helixi363 – 37412
Helixi380 – 39213
Beta strandi394 – 3974
Helixi399 – 41618
Helixi422 – 43817
Helixi440 – 4445
Beta strandi447 – 4493
Helixi464 – 48522
Helixi486 – 4883
Beta strandi491 – 4955
Helixi500 – 51415
Turni517 – 5204
Helixi521 – 5233
Helixi524 – 53714
Helixi544 – 56320
Helixi564 – 5674
Helixi571 – 59222
Helixi597 – 61620
Helixi625 – 63915
Turni640 – 6434
Helixi644 – 6485
Helixi651 – 6599
Helixi661 – 6633
Helixi664 – 68017
Helixi682 – 6854
Helixi686 – 70116
Beta strandi703 – 7053
Helixi709 – 72416
Helixi725 – 7284
Helixi729 – 74315
Helixi753 – 77725
Beta strandi779 – 7824
Helixi785 – 7906
Helixi793 – 80614
Helixi812 – 82817
Helixi834 – 8385
Helixi841 – 8499
Helixi856 – 87116
Helixi872 – 8754

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCJX-ray2.60A/B1-449[»]
1UKLX-ray3.00A/B1-876[»]
ProteinModelPortaliP70168.
SMRiP70168. Positions 1-876.

Miscellaneous databases

EvolutionaryTraceiP70168.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10181Importin N-terminal
Add
BLAST
Repeati124 – 16340HEAT 1
Add
BLAST
Repeati168 – 20740HEAT 2
Add
BLAST
Repeati213 – 25038HEAT 3
Add
BLAST
Repeati317 – 35741HEAT 4
Add
BLAST
Repeati362 – 39938HEAT 5
Add
BLAST
Repeati404 – 44138HEAT 6
Add
BLAST
Repeati602 – 64140HEAT 7
Add
BLAST
Repeati687 – 72640HEAT 8
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 34214IAB-binding
Add
BLAST
Regioni334 – 41986Ran-GTP binding By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3415Poly-Asp

Sequence similaritiesi

Belongs to the importin beta family.
Contains 8 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiQ6GTI5.
KOiK14293.
OMAiGLTIMGM.
OrthoDBiEOG7GN2KZ.
TreeFamiTF105655.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view]
PANTHERiPTHR10527:SF1. PTHR10527:SF1. 1 hit.
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70168-1 [UniParc]FASTAAdd to Basket

« Hide

MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS    50
QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG 100
TETYRPSSAS QCVAGIACAE IPVSQWPELI PQLVANVTNP NSTEHMKEST 150
LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN 200
SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ 250
YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA 300
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV 350
CLMLLSTCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN 400
QLKPLVIQAM PTLIELMKDP SVVVRDTTAW TVGRICELLP EAAINDVYLA 450
PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS 500
SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT 550
TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA 600
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF 650
KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL 700
GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS 750
DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF 800
IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG 850
RRSKTNKAKT LATWATKELR KLKNQA 876
Length:876
Mass (Da):97,184
Last modified:July 27, 2011 - v2
Checksum:i00D6B15023A30598
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3881M → V in BAA11034. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D67015 mRNA. Translation: BAA11034.1.
D45836 mRNA. Translation: BAA08273.1.
AL627445 Genomic DNA. Translation: CAM26994.1.
CH466556 Genomic DNA. Translation: EDL16074.1.
BC052438 mRNA. Translation: AAH52438.1.
BC055115 mRNA. Translation: AAH55115.1.
CCDSiCCDS25316.1.
PIRiS66288.
RefSeqiNP_032405.3. NM_008379.3.
UniGeneiMm.251013.

Genome annotation databases

EnsembliENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
GeneIDi16211.
KEGGimmu:16211.
UCSCiuc007ldu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D67015 mRNA. Translation: BAA11034.1 .
D45836 mRNA. Translation: BAA08273.1 .
AL627445 Genomic DNA. Translation: CAM26994.1 .
CH466556 Genomic DNA. Translation: EDL16074.1 .
BC052438 mRNA. Translation: AAH52438.1 .
BC055115 mRNA. Translation: AAH55115.1 .
CCDSi CCDS25316.1.
PIRi S66288.
RefSeqi NP_032405.3. NM_008379.3.
UniGenei Mm.251013.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GCJ X-ray 2.60 A/B 1-449 [» ]
1UKL X-ray 3.00 A/B 1-876 [» ]
ProteinModelPortali P70168.
SMRi P70168. Positions 1-876.
ModBasei Search...

Protein-protein interaction databases

BioGridi 200654. 8 interactions.
DIPi DIP-33404N.
IntActi P70168. 13 interactions.
MINTi MINT-6478527.

PTM databases

PhosphoSitei P70168.

Proteomic databases

MaxQBi P70168.
PaxDbi P70168.
PRIDEi P70168.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001479 ; ENSMUSP00000001479 ; ENSMUSG00000001440 .
GeneIDi 16211.
KEGGi mmu:16211.
UCSCi uc007ldu.1. mouse.

Organism-specific databases

CTDi 3837.
MGIi MGI:107532. Kpnb1.

Phylogenomic databases

eggNOGi COG5215.
GeneTreei ENSGT00550000074898.
HOGENOMi HOG000204108.
HOVERGENi HBG002369.
InParanoidi Q6GTI5.
KOi K14293.
OMAi GLTIMGM.
OrthoDBi EOG7GN2KZ.
TreeFami TF105655.

Enzyme and pathway databases

Reactomei REACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_205002. Activation of DNA fragmentation factor.

Miscellaneous databases

ChiTaRSi KPNB1. mouse.
EvolutionaryTracei P70168.
NextBioi 289169.
PROi P70168.
SOURCEi Search...

Gene expression databases

ArrayExpressi P70168.
Bgeei P70168.
CleanExi MM_KPNB1.
Genevestigatori P70168.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view ]
PANTHERi PTHR10527:SF1. PTHR10527:SF1. 1 hit.
Pfami PF03810. IBN_N. 1 hit.
[Graphical view ]
SMARTi SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1."
    Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.
    Genomics 36:213-215(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile."
    Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.
    FEBS Lett. 368:415-419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92; 212-221; 377-389 AND 860-867.
    Strain: C57BL/6.
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Limb.
  6. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  7. "The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1."
    Forwood J.K., Harley V., Jans D.A.
    J. Biol. Chem. 276:46575-46582(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRY.
  8. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. "Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse."
    Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.
    J. Biol. Chem. 285:33113-33122(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KPNA7.
    Strain: C57BL/6.
    Tissue: Ovary.

Entry informationi

Entry nameiIMB1_MOUSE
AccessioniPrimary (citable) accession number: P70168
Secondary accession number(s): Q62117, Q6GTI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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