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P70168

- IMB1_MOUSE

UniProt

P70168 - IMB1_MOUSE

Protein

Importin subunit beta-1

Gene

Kpnb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. protein transporter activity Source: MGI

    GO - Biological processi

    1. protein import into nucleus Source: MGI
    2. ribosomal protein import into nucleus Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
    REACT_205002. Activation of DNA fragmentation factor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Importin subunit beta-1
    Alternative name(s):
    Karyopherin subunit beta-1
    Nuclear factor p97
    Pore targeting complex 97 kDa subunit
    Short name:
    PTAC97
    SCG
    Gene namesi
    Name:Kpnb1
    Synonyms:Impnb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:107532. Kpnb1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus envelope By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear membrane Source: Ensembl
    3. protein complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 876876Importin subunit beta-1PRO_0000120746Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei12 – 121PhosphoserineBy similarity
    Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei211 – 2111N6-acetyllysine; alternateBy similarity
    Cross-linki211 – 211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei835 – 8351N6-acetyllysineBy similarity
    Modified residuei867 – 8671N6-acetyllysineBy similarity

    Post-translational modificationi

    Mono-ADP-ribosylated by PARP16.By similarity

    Keywords - PTMi

    Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP70168.
    PaxDbiP70168.
    PRIDEiP70168.

    PTM databases

    PhosphoSiteiP70168.

    Expressioni

    Gene expression databases

    ArrayExpressiP70168.
    BgeeiP70168.
    CleanExiMM_KPNB1.
    GenevestigatoriP70168.

    Interactioni

    Subunit structurei

    Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones By similarity. Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota By similarity. Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) By similarity. Interacts with SLC35G1 and STIM1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200654. 8 interactions.
    DIPiDIP-33404N.
    IntActiP70168. 13 interactions.
    MINTiMINT-6478527.

    Structurei

    Secondary structure

    876
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 77
    Turni8 – 114
    Helixi15 – 4531
    Beta strandi47 – 493
    Helixi51 – 6212
    Turni63 – 653
    Helixi70 – 8213
    Helixi85 – 9814
    Beta strandi104 – 1063
    Helixi108 – 12013
    Helixi121 – 1233
    Helixi129 – 13810
    Helixi144 – 16017
    Helixi163 – 1664
    Helixi167 – 1693
    Helixi170 – 18112
    Helixi188 – 20114
    Turni202 – 2043
    Helixi206 – 2094
    Helixi212 – 22514
    Helixi231 – 24717
    Helixi249 – 2546
    Turni255 – 2595
    Helixi260 – 2689
    Helixi273 – 30230
    Helixi314 – 32916
    Helixi330 – 3323
    Beta strandi338 – 3403
    Helixi344 – 35916
    Helixi360 – 3623
    Helixi363 – 37412
    Helixi380 – 39213
    Beta strandi394 – 3974
    Helixi399 – 41618
    Helixi422 – 43817
    Helixi440 – 4445
    Beta strandi447 – 4493
    Helixi464 – 48522
    Helixi486 – 4883
    Beta strandi491 – 4955
    Helixi500 – 51415
    Turni517 – 5204
    Helixi521 – 5233
    Helixi524 – 53714
    Helixi544 – 56320
    Helixi564 – 5674
    Helixi571 – 59222
    Helixi597 – 61620
    Helixi625 – 63915
    Turni640 – 6434
    Helixi644 – 6485
    Helixi651 – 6599
    Helixi661 – 6633
    Helixi664 – 68017
    Helixi682 – 6854
    Helixi686 – 70116
    Beta strandi703 – 7053
    Helixi709 – 72416
    Helixi725 – 7284
    Helixi729 – 74315
    Helixi753 – 77725
    Beta strandi779 – 7824
    Helixi785 – 7906
    Helixi793 – 80614
    Helixi812 – 82817
    Helixi834 – 8385
    Helixi841 – 8499
    Helixi856 – 87116
    Helixi872 – 8754

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GCJX-ray2.60A/B1-449[»]
    1UKLX-ray3.00A/B1-876[»]
    ProteinModelPortaliP70168.
    SMRiP70168. Positions 1-876.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP70168.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 10181Importin N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Repeati124 – 16340HEAT 1Add
    BLAST
    Repeati168 – 20740HEAT 2Add
    BLAST
    Repeati213 – 25038HEAT 3Add
    BLAST
    Repeati317 – 35741HEAT 4Add
    BLAST
    Repeati362 – 39938HEAT 5Add
    BLAST
    Repeati404 – 44138HEAT 6Add
    BLAST
    Repeati602 – 64140HEAT 7Add
    BLAST
    Repeati687 – 72640HEAT 8Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni329 – 34214IAB-bindingAdd
    BLAST
    Regioni334 – 41986Ran-GTP bindingBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi337 – 3415Poly-Asp

    Sequence similaritiesi

    Belongs to the importin beta family.Curated
    Contains 8 HEAT repeats.PROSITE-ProRule annotation
    Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5215.
    GeneTreeiENSGT00550000074898.
    HOGENOMiHOG000204108.
    HOVERGENiHBG002369.
    InParanoidiQ6GTI5.
    KOiK14293.
    OMAiGLTIMGM.
    OrthoDBiEOG7GN2KZ.
    TreeFamiTF105655.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR021133. HEAT_type_2.
    IPR001494. Importin-beta_N.
    IPR027140. KPNB1.
    [Graphical view]
    PANTHERiPTHR10527:SF1. PTHR10527:SF1. 1 hit.
    PfamiPF03810. IBN_N. 1 hit.
    [Graphical view]
    SMARTiSM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50077. HEAT_REPEAT. 1 hit.
    PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70168-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS    50
    QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG 100
    TETYRPSSAS QCVAGIACAE IPVSQWPELI PQLVANVTNP NSTEHMKEST 150
    LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN 200
    SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ 250
    YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA 300
    AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV 350
    CLMLLSTCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN 400
    QLKPLVIQAM PTLIELMKDP SVVVRDTTAW TVGRICELLP EAAINDVYLA 450
    PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS 500
    SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT 550
    TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA 600
    LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF 650
    KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL 700
    GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS 750
    DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF 800
    IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG 850
    RRSKTNKAKT LATWATKELR KLKNQA 876
    Length:876
    Mass (Da):97,184
    Last modified:July 27, 2011 - v2
    Checksum:i00D6B15023A30598
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti388 – 3881M → V in BAA11034. (PubMed:8812441)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67015 mRNA. Translation: BAA11034.1.
    D45836 mRNA. Translation: BAA08273.1.
    AL627445 Genomic DNA. Translation: CAM26994.1.
    CH466556 Genomic DNA. Translation: EDL16074.1.
    BC052438 mRNA. Translation: AAH52438.1.
    BC055115 mRNA. Translation: AAH55115.1.
    CCDSiCCDS25316.1.
    PIRiS66288.
    RefSeqiNP_032405.3. NM_008379.3.
    UniGeneiMm.251013.

    Genome annotation databases

    EnsembliENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
    GeneIDi16211.
    KEGGimmu:16211.
    UCSCiuc007ldu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D67015 mRNA. Translation: BAA11034.1 .
    D45836 mRNA. Translation: BAA08273.1 .
    AL627445 Genomic DNA. Translation: CAM26994.1 .
    CH466556 Genomic DNA. Translation: EDL16074.1 .
    BC052438 mRNA. Translation: AAH52438.1 .
    BC055115 mRNA. Translation: AAH55115.1 .
    CCDSi CCDS25316.1.
    PIRi S66288.
    RefSeqi NP_032405.3. NM_008379.3.
    UniGenei Mm.251013.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GCJ X-ray 2.60 A/B 1-449 [» ]
    1UKL X-ray 3.00 A/B 1-876 [» ]
    ProteinModelPortali P70168.
    SMRi P70168. Positions 1-876.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200654. 8 interactions.
    DIPi DIP-33404N.
    IntActi P70168. 13 interactions.
    MINTi MINT-6478527.

    PTM databases

    PhosphoSitei P70168.

    Proteomic databases

    MaxQBi P70168.
    PaxDbi P70168.
    PRIDEi P70168.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001479 ; ENSMUSP00000001479 ; ENSMUSG00000001440 .
    GeneIDi 16211.
    KEGGi mmu:16211.
    UCSCi uc007ldu.1. mouse.

    Organism-specific databases

    CTDi 3837.
    MGIi MGI:107532. Kpnb1.

    Phylogenomic databases

    eggNOGi COG5215.
    GeneTreei ENSGT00550000074898.
    HOGENOMi HOG000204108.
    HOVERGENi HBG002369.
    InParanoidi Q6GTI5.
    KOi K14293.
    OMAi GLTIMGM.
    OrthoDBi EOG7GN2KZ.
    TreeFami TF105655.

    Enzyme and pathway databases

    Reactomei REACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
    REACT_205002. Activation of DNA fragmentation factor.

    Miscellaneous databases

    ChiTaRSi KPNB1. mouse.
    EvolutionaryTracei P70168.
    NextBioi 289169.
    PROi P70168.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70168.
    Bgeei P70168.
    CleanExi MM_KPNB1.
    Genevestigatori P70168.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR021133. HEAT_type_2.
    IPR001494. Importin-beta_N.
    IPR027140. KPNB1.
    [Graphical view ]
    PANTHERi PTHR10527:SF1. PTHR10527:SF1. 1 hit.
    Pfami PF03810. IBN_N. 1 hit.
    [Graphical view ]
    SMARTi SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50077. HEAT_REPEAT. 1 hit.
    PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1."
      Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.
      Genomics 36:213-215(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile."
      Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.
      FEBS Lett. 368:415-419(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92; 212-221; 377-389 AND 860-867.
      Strain: C57BL/6.
      Tissue: Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Limb.
    6. "Multiple pathways contribute to nuclear import of core histones."
      Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
      EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
    7. "The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1."
      Forwood J.K., Harley V., Jans D.A.
      J. Biol. Chem. 276:46575-46582(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRY.
    8. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    9. "Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse."
      Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.
      J. Biol. Chem. 285:33113-33122(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KPNA7.
      Strain: C57BL/6.
      Tissue: Ovary.

    Entry informationi

    Entry nameiIMB1_MOUSE
    AccessioniPrimary (citable) accession number: P70168
    Secondary accession number(s): Q62117, Q6GTI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3