Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70168

- IMB1_MOUSE

UniProt

P70168 - IMB1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Importin subunit beta-1

Gene

Kpnb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. protein transporter activity Source: MGI

GO - Biological processi

  1. protein import into nucleus Source: MGI
  2. ribosomal protein import into nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_205002. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1
Alternative name(s):
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name:
PTAC97
SCG
Gene namesi
Name:Kpnb1
Synonyms:Impnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:107532. Kpnb1.

Subcellular locationi

Cytoplasm By similarity. Nucleus envelope By similarity

GO - Cellular componenti

  1. endoplasmic reticulum tubular network Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
  3. nuclear membrane Source: Ensembl
  4. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Importin subunit beta-1PRO_0000120746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei211 – 2111N6-acetyllysine; alternateBy similarity
Cross-linki211 – 211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei835 – 8351N6-acetyllysineBy similarity
Modified residuei867 – 8671N6-acetyllysineBy similarity

Post-translational modificationi

Mono-ADP-ribosylated by PARP16.By similarity

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP70168.
PaxDbiP70168.
PRIDEiP70168.

PTM databases

PhosphoSiteiP70168.

Expressioni

Gene expression databases

BgeeiP70168.
CleanExiMM_KPNB1.
ExpressionAtlasiP70168. baseline and differential.
GenevestigatoriP70168.

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota (By similarity). Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) (By similarity). Interacts with SLC35G1 and STIM1 (By similarity). Interacts with DCAF8.By similarity

Protein-protein interaction databases

BioGridi200654. 8 interactions.
DIPiDIP-33404N.
IntActiP70168. 13 interactions.
MINTiMINT-6478527.

Structurei

Secondary structure

876
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 77Combined sources
Turni8 – 114Combined sources
Helixi15 – 4531Combined sources
Beta strandi47 – 493Combined sources
Helixi51 – 6212Combined sources
Turni63 – 653Combined sources
Helixi70 – 8213Combined sources
Helixi85 – 9814Combined sources
Beta strandi104 – 1063Combined sources
Helixi108 – 12013Combined sources
Helixi121 – 1233Combined sources
Helixi129 – 13810Combined sources
Helixi144 – 16017Combined sources
Helixi163 – 1664Combined sources
Helixi167 – 1693Combined sources
Helixi170 – 18112Combined sources
Helixi188 – 20114Combined sources
Turni202 – 2043Combined sources
Helixi206 – 2094Combined sources
Helixi212 – 22514Combined sources
Helixi231 – 24717Combined sources
Helixi249 – 2546Combined sources
Turni255 – 2595Combined sources
Helixi260 – 2689Combined sources
Helixi273 – 30230Combined sources
Helixi314 – 32916Combined sources
Helixi330 – 3323Combined sources
Beta strandi338 – 3403Combined sources
Helixi344 – 35916Combined sources
Helixi360 – 3623Combined sources
Helixi363 – 37412Combined sources
Helixi380 – 39213Combined sources
Beta strandi394 – 3974Combined sources
Helixi399 – 41618Combined sources
Helixi422 – 43817Combined sources
Helixi440 – 4445Combined sources
Beta strandi447 – 4493Combined sources
Helixi464 – 48522Combined sources
Helixi486 – 4883Combined sources
Beta strandi491 – 4955Combined sources
Helixi500 – 51415Combined sources
Turni517 – 5204Combined sources
Helixi521 – 5233Combined sources
Helixi524 – 53714Combined sources
Helixi544 – 56320Combined sources
Helixi564 – 5674Combined sources
Helixi571 – 59222Combined sources
Helixi597 – 61620Combined sources
Helixi625 – 63915Combined sources
Turni640 – 6434Combined sources
Helixi644 – 6485Combined sources
Helixi651 – 6599Combined sources
Helixi661 – 6633Combined sources
Helixi664 – 68017Combined sources
Helixi682 – 6854Combined sources
Helixi686 – 70116Combined sources
Beta strandi703 – 7053Combined sources
Helixi709 – 72416Combined sources
Helixi725 – 7284Combined sources
Helixi729 – 74315Combined sources
Helixi753 – 77725Combined sources
Beta strandi779 – 7824Combined sources
Helixi785 – 7906Combined sources
Helixi793 – 80614Combined sources
Helixi812 – 82817Combined sources
Helixi834 – 8385Combined sources
Helixi841 – 8499Combined sources
Helixi856 – 87116Combined sources
Helixi872 – 8754Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCJX-ray2.60A/B1-449[»]
1UKLX-ray3.00A/B1-876[»]
ProteinModelPortaliP70168.
SMRiP70168. Positions 1-876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70168.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10181Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati124 – 16340HEAT 1Add
BLAST
Repeati168 – 20740HEAT 2Add
BLAST
Repeati213 – 25038HEAT 3Add
BLAST
Repeati317 – 35741HEAT 4Add
BLAST
Repeati362 – 39938HEAT 5Add
BLAST
Repeati404 – 44138HEAT 6Add
BLAST
Repeati602 – 64140HEAT 7Add
BLAST
Repeati687 – 72640HEAT 8Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 34214IAB-bindingAdd
BLAST
Regioni334 – 41986Ran-GTP bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3415Poly-Asp

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 8 HEAT repeats.PROSITE-ProRule annotation
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5215.
GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiP70168.
KOiK14293.
OMAiGLTIMGM.
OrthoDBiEOG7GN2KZ.
TreeFamiTF105655.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view]
PANTHERiPTHR10527:SF1. PTHR10527:SF1. 1 hit.
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70168-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS
60 70 80 90 100
QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG
110 120 130 140 150
TETYRPSSAS QCVAGIACAE IPVSQWPELI PQLVANVTNP NSTEHMKEST
160 170 180 190 200
LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN
210 220 230 240 250
SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ
260 270 280 290 300
YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA
310 320 330 340 350
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV
360 370 380 390 400
CLMLLSTCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GSILEGPEPN
410 420 430 440 450
QLKPLVIQAM PTLIELMKDP SVVVRDTTAW TVGRICELLP EAAINDVYLA
460 470 480 490 500
PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS
510 520 530 540 550
SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT
560 570 580 590 600
TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA
610 620 630 640 650
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF
660 670 680 690 700
KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNILPFCD EVMQLLLENL
710 720 730 740 750
GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS
760 770 780 790 800
DFDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF
810 820 830 840 850
IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG
860 870
RRSKTNKAKT LATWATKELR KLKNQA
Length:876
Mass (Da):97,184
Last modified:July 27, 2011 - v2
Checksum:i00D6B15023A30598
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti388 – 3881M → V in BAA11034. (PubMed:8812441)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67015 mRNA. Translation: BAA11034.1.
D45836 mRNA. Translation: BAA08273.1.
AL627445 Genomic DNA. Translation: CAM26994.1.
CH466556 Genomic DNA. Translation: EDL16074.1.
BC052438 mRNA. Translation: AAH52438.1.
BC055115 mRNA. Translation: AAH55115.1.
CCDSiCCDS25316.1.
PIRiS66288.
RefSeqiNP_032405.3. NM_008379.3.
UniGeneiMm.251013.

Genome annotation databases

EnsembliENSMUST00000001479; ENSMUSP00000001479; ENSMUSG00000001440.
GeneIDi16211.
KEGGimmu:16211.
UCSCiuc007ldu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D67015 mRNA. Translation: BAA11034.1 .
D45836 mRNA. Translation: BAA08273.1 .
AL627445 Genomic DNA. Translation: CAM26994.1 .
CH466556 Genomic DNA. Translation: EDL16074.1 .
BC052438 mRNA. Translation: AAH52438.1 .
BC055115 mRNA. Translation: AAH55115.1 .
CCDSi CCDS25316.1.
PIRi S66288.
RefSeqi NP_032405.3. NM_008379.3.
UniGenei Mm.251013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GCJ X-ray 2.60 A/B 1-449 [» ]
1UKL X-ray 3.00 A/B 1-876 [» ]
ProteinModelPortali P70168.
SMRi P70168. Positions 1-876.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200654. 8 interactions.
DIPi DIP-33404N.
IntActi P70168. 13 interactions.
MINTi MINT-6478527.

PTM databases

PhosphoSitei P70168.

Proteomic databases

MaxQBi P70168.
PaxDbi P70168.
PRIDEi P70168.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001479 ; ENSMUSP00000001479 ; ENSMUSG00000001440 .
GeneIDi 16211.
KEGGi mmu:16211.
UCSCi uc007ldu.1. mouse.

Organism-specific databases

CTDi 3837.
MGIi MGI:107532. Kpnb1.

Phylogenomic databases

eggNOGi COG5215.
GeneTreei ENSGT00550000074898.
HOGENOMi HOG000204108.
HOVERGENi HBG002369.
InParanoidi P70168.
KOi K14293.
OMAi GLTIMGM.
OrthoDBi EOG7GN2KZ.
TreeFami TF105655.

Enzyme and pathway databases

Reactomei REACT_198968. Regulation of cholesterol biosynthesis by SREBP (SREBF).
REACT_205002. Activation of DNA fragmentation factor.

Miscellaneous databases

ChiTaRSi Kpnb1. mouse.
EvolutionaryTracei P70168.
NextBioi 289169.
PROi P70168.
SOURCEi Search...

Gene expression databases

Bgeei P70168.
CleanExi MM_KPNB1.
ExpressionAtlasi P70168. baseline and differential.
Genevestigatori P70168.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
IPR027140. KPNB1.
[Graphical view ]
PANTHERi PTHR10527:SF1. PTHR10527:SF1. 1 hit.
Pfami PF03810. IBN_N. 1 hit.
[Graphical view ]
SMARTi SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1."
    Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.
    Genomics 36:213-215(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile."
    Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.
    FEBS Lett. 368:415-419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 74-92; 212-221; 377-389 AND 860-867.
    Strain: C57BL/6.
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Limb.
  6. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  7. "The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1."
    Forwood J.K., Harley V., Jans D.A.
    J. Biol. Chem. 276:46575-46582(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRY.
  8. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. "Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse."
    Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.
    J. Biol. Chem. 285:33113-33122(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KPNA7.
    Strain: C57BL/6.
    Tissue: Ovary.

Entry informationi

Entry nameiIMB1_MOUSE
AccessioniPrimary (citable) accession number: P70168
Secondary accession number(s): Q62117, Q6GTI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3