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Protein

Alpha-2,8-sialyltransferase 8E

Gene

St8sia5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281SubstrateBy similarity
Active sitei384 – 3841Proton donor/acceptorBy similarity

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry

SwissLipidsiSLP:000000750. [P70126-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8E (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8E
Short name:
SIAT8-E
Sialyltransferase St8Sia V
Short name:
ST8SiaV
Gene namesi
Name:St8sia5
Synonyms:Siat8e, St8siav
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:109243. St8sia5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini38 – 412375LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Alpha-2,8-sialyltransferase 8EPRO_0000149297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence analysis
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence analysis
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Disulfide bondi200 ↔ 349By similarity
Disulfide bondi214 ↔ 409By similarity
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP70126.
PRIDEiP70126.

PTM databases

iPTMnetiP70126.
PhosphoSiteiP70126.

Expressioni

Tissue specificityi

Highly expressed in brain. Expressed at low levels in other tissues, including liver, testis, lung, placenta and spleen.

Gene expression databases

BgeeiP70126.
ExpressionAtlasiP70126. baseline and differential.
GenevisibleiP70126. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074764.

Structurei

3D structure databases

ProteinModelPortaliP70126.
SMRiP70126. Positions 128-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni250 – 2523Substrate bindingBy similarity
Regioni336 – 3383Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP70126.
KOiK03369.
OMAiIFITHHY.
OrthoDBiEOG7SV0V9.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform V-L (identifier: P70126-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRYADPSANR DLLGNRTLLF IFICAFALVT LLQQILYSKS YIKRGFQFGW
60 70 80 90 100
QRGDQQANWT GLFNDSDSPT EQNITGSSSR YFEFYKEPLE FNSTRCLELR
110 120 130 140 150
QEILEVKVLS MVKQSELFER WKSLQICKWA MDTAEASLFK STLSRCCNAP
160 170 180 190 200
SFLFTTQKNT PVETNLRYEV ESSGLYHIDQ EIFKMFPKEM PYYRSQFKKC
210 220 230 240 250
AVVGNGGILK NSGCGKEINS ADFVFRCNLP PISGIYTTDV GEKTDVVTVN
260 270 280 290 300
PSIIIDRFHK LEKWRRPFFS VLQRYENASV LLPAFYNVRN TLVSFRVKYM
310 320 330 340 350
LDDFQSRQPV YFFHPQYLSS VSRYWLSLGV RARRISTGLI LVTAALELCE
360 370 380 390 400
EVHLFGFWAF PMNPSGFFIT HHYYDNVKPK PGFHAMPSEI FTFLRMHSRG
410
ILRVHTGTCN CC
Length:412
Mass (Da):47,829
Last modified:July 27, 2011 - v2
Checksum:i1BBF9256017F7305
GO
Isoform V-M (identifier: P70126-2) [UniParc]FASTAAdd to basket

Also known as: Middle

The sequence of this isoform differs from the canonical sequence as follows:
     44-79: Missing.

Show »
Length:376
Mass (Da):43,827
Checksum:i3EAD07C8034EBC04
GO
Isoform V-S (identifier: P70126-3) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     44-110: Missing.

Show »
Length:345
Mass (Da):39,964
Checksum:i25EC36859A4016D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1343DTA → GAS in CAA66642 (PubMed:8910600).Curated
Sequence conflicti132 – 1343DTA → GAS in CAA66643 (PubMed:8910600).Curated
Sequence conflicti132 – 1343DTA → GAS in CAA66644 (PubMed:8910600).Curated
Sequence conflicti151 – 1511S → N in CAA66642 (PubMed:8910600).Curated
Sequence conflicti151 – 1511S → N in CAA66643 (PubMed:8910600).Curated
Sequence conflicti151 – 1511S → N in CAA66644 (PubMed:8910600).Curated
Sequence conflicti340 – 3401I → S in CAA66642 (PubMed:8910600).Curated
Sequence conflicti340 – 3401I → S in CAA66643 (PubMed:8910600).Curated
Sequence conflicti340 – 3401I → S in CAA66644 (PubMed:8910600).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei44 – 11067Missing in isoform V-S. CuratedVSP_001790Add
BLAST
Alternative sequencei44 – 7936Missing in isoform V-M. CuratedVSP_001789Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98014 mRNA. Translation: CAA66642.1.
X98014 mRNA. Translation: CAA66643.1.
X98014 mRNA. Translation: CAA66644.1.
AC157991 Genomic DNA. No translation available.
CCDSiCCDS29353.1. [P70126-2]
CCDS50325.1. [P70126-1]
RefSeqiNP_038694.2. NM_013666.2. [P70126-1]
UniGeneiMm.365178.

Genome annotation databases

EnsembliENSMUST00000075290; ENSMUSP00000074764; ENSMUSG00000025425. [P70126-1]
ENSMUST00000079618; ENSMUSP00000078566; ENSMUSG00000025425. [P70126-2]
GeneIDi225742.
KEGGimmu:225742.
UCSCiuc008frh.2. mouse. [P70126-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia V

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98014 mRNA. Translation: CAA66642.1.
X98014 mRNA. Translation: CAA66643.1.
X98014 mRNA. Translation: CAA66644.1.
AC157991 Genomic DNA. No translation available.
CCDSiCCDS29353.1. [P70126-2]
CCDS50325.1. [P70126-1]
RefSeqiNP_038694.2. NM_013666.2. [P70126-1]
UniGeneiMm.365178.

3D structure databases

ProteinModelPortaliP70126.
SMRiP70126. Positions 128-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074764.

Chemistry

SwissLipidsiSLP:000000750. [P70126-2]

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiP70126.
PhosphoSiteiP70126.

Proteomic databases

PaxDbiP70126.
PRIDEiP70126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075290; ENSMUSP00000074764; ENSMUSG00000025425. [P70126-1]
ENSMUST00000079618; ENSMUSP00000078566; ENSMUSG00000025425. [P70126-2]
GeneIDi225742.
KEGGimmu:225742.
UCSCiuc008frh.2. mouse. [P70126-1]

Organism-specific databases

CTDi29906.
MGIiMGI:109243. St8sia5.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP70126.
KOiK03369.
OMAiIFITHHY.
OrthoDBiEOG7SV0V9.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiP70126.
SOURCEiSearch...

Gene expression databases

BgeeiP70126.
ExpressionAtlasiP70126. baseline and differential.
GenevisibleiP70126. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3."
    Kono M., Yoshida Y., Kojima N., Tsuji S.
    J. Biol. Chem. 271:29366-29371(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, ALTERNATIVE SPLICING.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiSIA8E_MOUSE
AccessioniPrimary (citable) accession number: P70126
Secondary accession number(s): E9QKY5, P70127, P70128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.