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Protein

Alpha-2,8-sialyltransferase 8E

Gene

St8sia5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228SubstrateBy similarity1
Active sitei384Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry databases

SwissLipidsiSLP:000000750. [P70126-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8E (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8E
Short name:
SIAT8-E
Sialyltransferase St8Sia V
Short name:
ST8SiaV
Gene namesi
Name:St8sia5
Synonyms:Siat8e, St8siav
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:109243. St8sia5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini38 – 412LumenalSequence analysisAdd BLAST375

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492971 – 412Alpha-2,8-sialyltransferase 8EAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi64N-linked (GlcNAc...)Sequence analysis1
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi200 ↔ 349By similarity
Disulfide bondi214 ↔ 409By similarity
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP70126.
PRIDEiP70126.

PTM databases

iPTMnetiP70126.
PhosphoSitePlusiP70126.

Expressioni

Tissue specificityi

Highly expressed in brain. Expressed at low levels in other tissues, including liver, testis, lung, placenta and spleen.

Gene expression databases

BgeeiENSMUSG00000025425.
ExpressionAtlasiP70126. baseline and differential.
GenevisibleiP70126. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074764.

Structurei

3D structure databases

ProteinModelPortaliP70126.
SMRiP70126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni250 – 252Substrate bindingBy similarity3
Regioni336 – 338Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP70126.
KOiK03369.
OMAiIFITHHY.
OrthoDBiEOG091G0CER.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform V-L (identifier: P70126-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRYADPSANR DLLGNRTLLF IFICAFALVT LLQQILYSKS YIKRGFQFGW
60 70 80 90 100
QRGDQQANWT GLFNDSDSPT EQNITGSSSR YFEFYKEPLE FNSTRCLELR
110 120 130 140 150
QEILEVKVLS MVKQSELFER WKSLQICKWA MDTAEASLFK STLSRCCNAP
160 170 180 190 200
SFLFTTQKNT PVETNLRYEV ESSGLYHIDQ EIFKMFPKEM PYYRSQFKKC
210 220 230 240 250
AVVGNGGILK NSGCGKEINS ADFVFRCNLP PISGIYTTDV GEKTDVVTVN
260 270 280 290 300
PSIIIDRFHK LEKWRRPFFS VLQRYENASV LLPAFYNVRN TLVSFRVKYM
310 320 330 340 350
LDDFQSRQPV YFFHPQYLSS VSRYWLSLGV RARRISTGLI LVTAALELCE
360 370 380 390 400
EVHLFGFWAF PMNPSGFFIT HHYYDNVKPK PGFHAMPSEI FTFLRMHSRG
410
ILRVHTGTCN CC
Length:412
Mass (Da):47,829
Last modified:July 27, 2011 - v2
Checksum:i1BBF9256017F7305
GO
Isoform V-M (identifier: P70126-2) [UniParc]FASTAAdd to basket
Also known as: Middle

The sequence of this isoform differs from the canonical sequence as follows:
     44-79: Missing.

Show »
Length:376
Mass (Da):43,827
Checksum:i3EAD07C8034EBC04
GO
Isoform V-S (identifier: P70126-3) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     44-110: Missing.

Show »
Length:345
Mass (Da):39,964
Checksum:i25EC36859A4016D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132 – 134DTA → GAS in CAA66642 (PubMed:8910600).Curated3
Sequence conflicti132 – 134DTA → GAS in CAA66643 (PubMed:8910600).Curated3
Sequence conflicti132 – 134DTA → GAS in CAA66644 (PubMed:8910600).Curated3
Sequence conflicti151S → N in CAA66642 (PubMed:8910600).Curated1
Sequence conflicti151S → N in CAA66643 (PubMed:8910600).Curated1
Sequence conflicti151S → N in CAA66644 (PubMed:8910600).Curated1
Sequence conflicti340I → S in CAA66642 (PubMed:8910600).Curated1
Sequence conflicti340I → S in CAA66643 (PubMed:8910600).Curated1
Sequence conflicti340I → S in CAA66644 (PubMed:8910600).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00179044 – 110Missing in isoform V-S. CuratedAdd BLAST67
Alternative sequenceiVSP_00178944 – 79Missing in isoform V-M. CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98014 mRNA. Translation: CAA66642.1.
X98014 mRNA. Translation: CAA66643.1.
X98014 mRNA. Translation: CAA66644.1.
AC157991 Genomic DNA. No translation available.
CCDSiCCDS29353.1. [P70126-2]
CCDS50325.1. [P70126-1]
RefSeqiNP_038694.2. NM_013666.2. [P70126-1]
UniGeneiMm.365178.

Genome annotation databases

EnsembliENSMUST00000075290; ENSMUSP00000074764; ENSMUSG00000025425. [P70126-1]
ENSMUST00000079618; ENSMUSP00000078566; ENSMUSG00000025425. [P70126-2]
GeneIDi225742.
KEGGimmu:225742.
UCSCiuc008frh.2. mouse. [P70126-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia V

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98014 mRNA. Translation: CAA66642.1.
X98014 mRNA. Translation: CAA66643.1.
X98014 mRNA. Translation: CAA66644.1.
AC157991 Genomic DNA. No translation available.
CCDSiCCDS29353.1. [P70126-2]
CCDS50325.1. [P70126-1]
RefSeqiNP_038694.2. NM_013666.2. [P70126-1]
UniGeneiMm.365178.

3D structure databases

ProteinModelPortaliP70126.
SMRiP70126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074764.

Chemistry databases

SwissLipidsiSLP:000000750. [P70126-2]

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiP70126.
PhosphoSitePlusiP70126.

Proteomic databases

PaxDbiP70126.
PRIDEiP70126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075290; ENSMUSP00000074764; ENSMUSG00000025425. [P70126-1]
ENSMUST00000079618; ENSMUSP00000078566; ENSMUSG00000025425. [P70126-2]
GeneIDi225742.
KEGGimmu:225742.
UCSCiuc008frh.2. mouse. [P70126-1]

Organism-specific databases

CTDi29906.
MGIiMGI:109243. St8sia5.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiP70126.
KOiK03369.
OMAiIFITHHY.
OrthoDBiEOG091G0CER.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiP70126.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025425.
ExpressionAtlasiP70126. baseline and differential.
GenevisibleiP70126. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8E_MOUSE
AccessioniPrimary (citable) accession number: P70126
Secondary accession number(s): E9QKY5, P70127, P70128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.