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Protein

Zinc fingers and homeoboxes protein 1

Gene

Zhx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Increases DNMT3B-mediated repressive transcriptional activity when DNMT3B is tethered to DNA. May link molecule between DNMT3B and other co-repressor proteins (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri70 – 93C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri102 – 125C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24
DNA bindingi284 – 346Homeobox 1PROSITE-ProRule annotationCuratedAdd BLAST63
DNA bindingi464 – 526Homeobox 2PROSITE-ProRule annotationCuratedAdd BLAST63
DNA bindingi569 – 630Homeobox 3PROSITE-ProRule annotationCuratedAdd BLAST62
DNA bindingi660 – 722Homeobox 4PROSITE-ProRule annotationCuratedAdd BLAST63
DNA bindingi777 – 832Homeobox 5PROSITE-ProRule annotationCuratedAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 1
Gene namesi
Name:Zhx1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109271. Zhx1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Colocalized in the nucleus with DNMT3B.By similarity

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000493891 – 873Zinc fingers and homeoboxes protein 1Add BLAST873

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphothreonineBy similarity1
Modified residuei45PhosphoserineCombined sources1
Modified residuei47PhosphoserineCombined sources1
Modified residuei48PhosphoserineCombined sources1
Modified residuei202PhosphoserineBy similarity1
Modified residuei648PhosphoserineCombined sources1
Modified residuei774PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70121.
MaxQBiP70121.
PaxDbiP70121.
PeptideAtlasiP70121.
PRIDEiP70121.

PTM databases

iPTMnetiP70121.
PhosphoSitePlusiP70121.

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain.1 Publication

Inductioni

By interleukin-2.1 Publication

Gene expression databases

BgeeiENSMUSG00000022361.
CleanExiMM_ZHX1.
ExpressionAtlasiP70121. baseline and differential.
GenevisibleiP70121. MM.

Interactioni

Subunit structurei

Forms homodimers. Heterodimer (via HD1 domain) with ZHX2 (via HD1 domain). Also forms a heterodimer with ZHX3 which is a prerequisite for repressor activity. Interacts with ATF7IP and NFYA. Interacts (via homeobox domains) with DNMT3B (via PWWP domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066201.

Structurei

3D structure databases

ProteinModelPortaliP70121.
SMRiP70121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni272 – 564Required for interaction with NFYABy similarityAdd BLAST293
Regioni272 – 432Required for dimerizationBy similarityAdd BLAST161
Regioni734 – 768Required for nuclear localizationBy similarityAdd BLAST35
Regioni831 – 873Required for repressor activityBy similarityAdd BLAST43

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 5 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri70 – 93C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri102 – 125C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOVERGENiHBG007920.
InParanoidiP70121.
OMAiSHMGYEQ.
OrthoDBiEOG091G02WT.
PhylomeDBiP70121.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 5 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR024578. Homez_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 4 hits.
PF11569. Homez. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 5 hits.
PROSITEiPS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRRKSTTP CMVLASEQDP DLELISDLDE GPPILTPVEN AKAESVSSDE
60 70 80 90 100
EVHGSVDSDN QQNKKVEGGY ECKYCTFQTP DLNMFTFHVD SEHPNVVLNS
110 120 130 140 150
SYVCVECNFL TKRYDALSEH NLKYHPGEEN FKLTMVKRNN QTIFEQTIND
160 170 180 190 200
LTFDGSFVKE ENTEQGESID VSSSGISISK TPIMKMMKNK VENKRITVHH
210 220 230 240 250
NSAEGTSEEK ENGVKASQEE NAESVSSSAL ESNTSTSTIN RVHPSPASTV
260 270 280 290 300
VTPTAVLPGL AQVITAVSAQ QNSNLLPKVL IPVNSIPTYN AALDNNPLLL
310 320 330 340 350
NTYNKFPYPT MSEITVLSAQ AKYTEEQIKI WFSAQRLKHG VSWTPEEVEE
360 370 380 390 400
ARRKQFNGTV HTVPQTITVI PTHISTGSNG LPSILQTCQI VGQPGLVLTQ
410 420 430 440 450
VAGTNTLPVT APIALTVAGV PNQTNVQKSQ VPAAQPATDT KPATAAVPSS
460 470 480 490 500
PSVRPEAALV NPDSFGIRAK KTKEQLAELK VSYLKNQFPH DSEIIRLMKI
510 520 530 540 550
TGLTKGEIKK WFSDTRYNQR NSKSNQCLHL NNDSSATIII DSSDETPEPP
560 570 580 590 600
AAAASQQKQS WNPFPDFAPQ KFKEKTAEQL RALQASFLNS SVLTDEEVNR
610 620 630 640 650
LRAQTKLTRR EIDAWFTEKN KTKALKDEKI EVDESNVGSS KEEPGESSPG
660 670 680 690 700
DETVAPKSGG TGKICKKTPE QLHMLKSAFV RTQWPSAEEY DKLAEESGLA
710 720 730 740 750
RTDIVSWFGD TRYAWKNGNL KWYYYYQSSN SSSLNGLSSL RRRGRGRPKG
760 770 780 790 800
RGRGRPRGRP RGGKRMNTWD RVPSLIKFKT GTAILKDYYL KHKFLNEQDL
810 820 830 840 850
DELVNRSHMG YEQVREWFAE RQRRSELGIE LFEENEEEDE VVDDQEEDEE
860 870
ETDDSDTWEP PRHVKRKLSK SDD
Length:873
Mass (Da):97,551
Last modified:February 16, 2004 - v2
Checksum:i98C5D5714D479364
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti375S → T (PubMed:8713137).Curated1
Sequence conflicti375S → T (PubMed:12527199).Curated1
Sequence conflicti388C → Y in BAC34629 (PubMed:16141072).Curated1
Sequence conflicti553A → R in CAA90905 (PubMed:8713137).Curated1
Sequence conflicti867 – 868KL → NV in CAA90905 (PubMed:8713137).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54200 mRNA. Translation: CAA90905.1.
AB078421 Genomic DNA. Translation: BAC22110.1.
AK051410 mRNA. Translation: BAC34629.1.
AK053191 mRNA. Translation: BAC35306.1.
BC054543 mRNA. Translation: AAH54543.1.
CCDSiCCDS27488.1.
PIRiJC4863.
RefSeqiNP_001035903.1. NM_001042438.2.
NP_033598.2. NM_009572.4.
UniGeneiMm.244931.

Genome annotation databases

EnsembliENSMUST00000070143; ENSMUSP00000066201; ENSMUSG00000022361.
ENSMUST00000110168; ENSMUSP00000105797; ENSMUSG00000022361.
ENSMUST00000175805; ENSMUSP00000134844; ENSMUSG00000022361.
GeneIDi22770.
KEGGimmu:22770.
UCSCiuc007vtd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54200 mRNA. Translation: CAA90905.1.
AB078421 Genomic DNA. Translation: BAC22110.1.
AK051410 mRNA. Translation: BAC34629.1.
AK053191 mRNA. Translation: BAC35306.1.
BC054543 mRNA. Translation: AAH54543.1.
CCDSiCCDS27488.1.
PIRiJC4863.
RefSeqiNP_001035903.1. NM_001042438.2.
NP_033598.2. NM_009572.4.
UniGeneiMm.244931.

3D structure databases

ProteinModelPortaliP70121.
SMRiP70121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066201.

PTM databases

iPTMnetiP70121.
PhosphoSitePlusiP70121.

Proteomic databases

EPDiP70121.
MaxQBiP70121.
PaxDbiP70121.
PeptideAtlasiP70121.
PRIDEiP70121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070143; ENSMUSP00000066201; ENSMUSG00000022361.
ENSMUST00000110168; ENSMUSP00000105797; ENSMUSG00000022361.
ENSMUST00000175805; ENSMUSP00000134844; ENSMUSG00000022361.
GeneIDi22770.
KEGGimmu:22770.
UCSCiuc007vtd.2. mouse.

Organism-specific databases

CTDi11244.
MGIiMGI:109271. Zhx1.

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOVERGENiHBG007920.
InParanoidiP70121.
OMAiSHMGYEQ.
OrthoDBiEOG091G02WT.
PhylomeDBiP70121.
TreeFamiTF333363.

Miscellaneous databases

PROiP70121.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022361.
CleanExiMM_ZHX1.
ExpressionAtlasiP70121. baseline and differential.
GenevisibleiP70121. MM.

Family and domain databases

Gene3Di1.10.10.60. 5 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR024578. Homez_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 4 hits.
PF11569. Homez. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 5 hits.
PROSITEiPS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZHX1_MOUSE
AccessioniPrimary (citable) accession number: P70121
Secondary accession number(s): Q8BQ68, Q8C6T4, Q8CJG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.