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Protein

Zinc fingers and homeoboxes protein 1

Gene

Zhx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Increases DNMT3B-mediated repressive transcriptional activity when DNMT3B is tethered to DNA. May link molecule between DNMT3B and other co-repressor proteins (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9324C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri102 – 12524C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi284 – 34663Homeobox 1PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi464 – 52663Homeobox 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi569 – 63062Homeobox 3PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi660 – 72263Homeobox 4PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi777 – 83256Homeobox 5PROSITE-ProRule annotationCuratedAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 1
Gene namesi
Name:Zhx1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109271. Zhx1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

  • Note: Colocalized in the nucleus with DNMT3B.By similarity

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Zinc fingers and homeoboxes protein 1PRO_0000049389Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphothreonineBy similarity
Modified residuei45 – 451PhosphoserineCombined sources
Modified residuei47 – 471PhosphoserineCombined sources
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei648 – 6481PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70121.
MaxQBiP70121.
PaxDbiP70121.
PRIDEiP70121.

PTM databases

iPTMnetiP70121.
PhosphoSiteiP70121.

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain.1 Publication

Inductioni

By interleukin-2.1 Publication

Gene expression databases

BgeeiP70121.
CleanExiMM_ZHX1.
ExpressionAtlasiP70121. baseline and differential.
GenevisibleiP70121. MM.

Interactioni

Subunit structurei

Forms homodimers. Heterodimer (via HD1 domain) with ZHX2 (via HD1 domain). Also forms a heterodimer with ZHX3 which is a prerequisite for repressor activity. Interacts with ATF7IP and NFYA. Interacts (via homeobox domains) with DNMT3B (via PWWP domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066201.

Structurei

3D structure databases

ProteinModelPortaliP70121.
SMRiP70121. Positions 60-153, 462-532, 565-636, 662-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni272 – 564293Required for interaction with NFYABy similarityAdd
BLAST
Regioni272 – 432161Required for dimerizationBy similarityAdd
BLAST
Regioni734 – 76835Required for nuclear localizationBy similarityAdd
BLAST
Regioni831 – 87343Required for repressor activityBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 5 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri70 – 9324C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri102 – 12524C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOVERGENiHBG007920.
InParanoidiP70121.
OMAiSHMGYEQ.
OrthoDBiEOG76HQ0T.
PhylomeDBiP70121.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 5 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR024578. Homez_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 4 hits.
PF11569. Homez. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 5 hits.
PROSITEiPS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRRKSTTP CMVLASEQDP DLELISDLDE GPPILTPVEN AKAESVSSDE
60 70 80 90 100
EVHGSVDSDN QQNKKVEGGY ECKYCTFQTP DLNMFTFHVD SEHPNVVLNS
110 120 130 140 150
SYVCVECNFL TKRYDALSEH NLKYHPGEEN FKLTMVKRNN QTIFEQTIND
160 170 180 190 200
LTFDGSFVKE ENTEQGESID VSSSGISISK TPIMKMMKNK VENKRITVHH
210 220 230 240 250
NSAEGTSEEK ENGVKASQEE NAESVSSSAL ESNTSTSTIN RVHPSPASTV
260 270 280 290 300
VTPTAVLPGL AQVITAVSAQ QNSNLLPKVL IPVNSIPTYN AALDNNPLLL
310 320 330 340 350
NTYNKFPYPT MSEITVLSAQ AKYTEEQIKI WFSAQRLKHG VSWTPEEVEE
360 370 380 390 400
ARRKQFNGTV HTVPQTITVI PTHISTGSNG LPSILQTCQI VGQPGLVLTQ
410 420 430 440 450
VAGTNTLPVT APIALTVAGV PNQTNVQKSQ VPAAQPATDT KPATAAVPSS
460 470 480 490 500
PSVRPEAALV NPDSFGIRAK KTKEQLAELK VSYLKNQFPH DSEIIRLMKI
510 520 530 540 550
TGLTKGEIKK WFSDTRYNQR NSKSNQCLHL NNDSSATIII DSSDETPEPP
560 570 580 590 600
AAAASQQKQS WNPFPDFAPQ KFKEKTAEQL RALQASFLNS SVLTDEEVNR
610 620 630 640 650
LRAQTKLTRR EIDAWFTEKN KTKALKDEKI EVDESNVGSS KEEPGESSPG
660 670 680 690 700
DETVAPKSGG TGKICKKTPE QLHMLKSAFV RTQWPSAEEY DKLAEESGLA
710 720 730 740 750
RTDIVSWFGD TRYAWKNGNL KWYYYYQSSN SSSLNGLSSL RRRGRGRPKG
760 770 780 790 800
RGRGRPRGRP RGGKRMNTWD RVPSLIKFKT GTAILKDYYL KHKFLNEQDL
810 820 830 840 850
DELVNRSHMG YEQVREWFAE RQRRSELGIE LFEENEEEDE VVDDQEEDEE
860 870
ETDDSDTWEP PRHVKRKLSK SDD
Length:873
Mass (Da):97,551
Last modified:February 16, 2004 - v2
Checksum:i98C5D5714D479364
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti375 – 3751S → T (PubMed:8713137).Curated
Sequence conflicti375 – 3751S → T (PubMed:12527199).Curated
Sequence conflicti388 – 3881C → Y in BAC34629 (PubMed:16141072).Curated
Sequence conflicti553 – 5531A → R in CAA90905 (PubMed:8713137).Curated
Sequence conflicti867 – 8682KL → NV in CAA90905 (PubMed:8713137).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54200 mRNA. Translation: CAA90905.1.
AB078421 Genomic DNA. Translation: BAC22110.1.
AK051410 mRNA. Translation: BAC34629.1.
AK053191 mRNA. Translation: BAC35306.1.
BC054543 mRNA. Translation: AAH54543.1.
CCDSiCCDS27488.1.
PIRiJC4863.
RefSeqiNP_001035903.1. NM_001042438.2.
NP_033598.2. NM_009572.4.
UniGeneiMm.244931.

Genome annotation databases

EnsembliENSMUST00000070143; ENSMUSP00000066201; ENSMUSG00000022361.
ENSMUST00000110168; ENSMUSP00000105797; ENSMUSG00000022361.
ENSMUST00000175805; ENSMUSP00000134844; ENSMUSG00000022361.
GeneIDi22770.
KEGGimmu:22770.
UCSCiuc007vtd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54200 mRNA. Translation: CAA90905.1.
AB078421 Genomic DNA. Translation: BAC22110.1.
AK051410 mRNA. Translation: BAC34629.1.
AK053191 mRNA. Translation: BAC35306.1.
BC054543 mRNA. Translation: AAH54543.1.
CCDSiCCDS27488.1.
PIRiJC4863.
RefSeqiNP_001035903.1. NM_001042438.2.
NP_033598.2. NM_009572.4.
UniGeneiMm.244931.

3D structure databases

ProteinModelPortaliP70121.
SMRiP70121. Positions 60-153, 462-532, 565-636, 662-731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066201.

PTM databases

iPTMnetiP70121.
PhosphoSiteiP70121.

Proteomic databases

EPDiP70121.
MaxQBiP70121.
PaxDbiP70121.
PRIDEiP70121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070143; ENSMUSP00000066201; ENSMUSG00000022361.
ENSMUST00000110168; ENSMUSP00000105797; ENSMUSG00000022361.
ENSMUST00000175805; ENSMUSP00000134844; ENSMUSG00000022361.
GeneIDi22770.
KEGGimmu:22770.
UCSCiuc007vtd.2. mouse.

Organism-specific databases

CTDi11244.
MGIiMGI:109271. Zhx1.

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOVERGENiHBG007920.
InParanoidiP70121.
OMAiSHMGYEQ.
OrthoDBiEOG76HQ0T.
PhylomeDBiP70121.
TreeFamiTF333363.

Miscellaneous databases

PROiP70121.
SOURCEiSearch...

Gene expression databases

BgeeiP70121.
CleanExiMM_ZHX1.
ExpressionAtlasiP70121. baseline and differential.
GenevisibleiP70121. MM.

Family and domain databases

Gene3Di1.10.10.60. 5 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR024578. Homez_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 4 hits.
PF11569. Homez. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 5 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 5 hits.
PROSITEiPS50071. HOMEOBOX_2. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "zhx-1: a novel mouse homeodomain protein containing two zinc-fingers and five homeodomains."
    Barthelemy I., Carramolino L., Gutierrez J., Barbero J.L., Marquez G., Zaballos A.
    Biochem. Biophys. Res. Commun. 224:870-876(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Bone marrow stroma1 Publication.
  2. "Genomic structure and analysis of transcriptional regulation of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene."
    Shou Z., Yamada K., Inazu T., Kawata H., Hirano S., Mizutani T., Yazawa T., Sekiguchi T., Yoshino M., Kajitani T., Okada K., Miyamoto K.
    Gene 302:83-94(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung and Spinal ganglion.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  5. "A mechanism of induction of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene expression by interleukin-2."
    Shou Z., Yamada K., Kawata H., Yokoyama O., Miyamoto K.
    Biochem. Biophys. Res. Commun. 314:885-890(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome."
    Liu G., Clement L.C., Kanwar Y.S., Avila-Casado C., Chugh S.S.
    J. Biol. Chem. 281:39681-39692(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Tissue: Kidney.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-47 AND SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-48 AND SER-648, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Spleen.

Entry informationi

Entry nameiZHX1_MOUSE
AccessioniPrimary (citable) accession number: P70121
Secondary accession number(s): Q8BQ68, Q8C6T4, Q8CJG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: June 8, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.