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P70120 (HES5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor HES-5
Alternative name(s):
Hairy and enhancer of split 5
Gene names
Name:Hes5
Synonyms:Hes-5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length167 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator. Ref.3

Subunit structure

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family By similarity.

Subcellular location

Nucleus.

Domain

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins By similarity.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Contains 1 Orange domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionDevelopmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Inferred from mutant phenotype PubMed 17331197. Source: UniProtKB

S-shaped body morphogenesis

Inferred from expression pattern PubMed 15821257. Source: UniProtKB

astrocyte differentiation

Inferred from direct assay PubMed 15156153. Source: UniProtKB

auditory receptor cell differentiation

Inferred from mutant phenotype PubMed 11425898. Source: MGI

auditory receptor cell fate determination

Inferred from mutant phenotype PubMed 11425898. Source: MGI

brain development

Inferred from mutant phenotype PubMed 17331197. Source: UniProtKB

camera-type eye development

Inferred from mutant phenotype PubMed 17331197. Source: UniProtKB

cartilage development

Inferred from mutant phenotype PubMed 17093926. Source: UniProtKB

cell adhesion

Inferred from genetic interaction PubMed 15496443. Source: MGI

cell maturation

Inferred from genetic interaction PubMed 15496443. Source: MGI

central nervous system myelination

Inferred from mutant phenotype PubMed 17006542. Source: MGI

comma-shaped body morphogenesis

Inferred from expression pattern PubMed 15821257. Source: UniProtKB

forebrain radial glial cell differentiation

Inferred from electronic annotation. Source: Ensembl

glial cell fate commitment

Inferred from mutant phenotype PubMed 10821751. Source: MGI

metanephric nephron tubule morphogenesis

Inferred from expression pattern PubMed 15465493. Source: UniProtKB

negative regulation of astrocyte differentiation

Inferred from direct assay PubMed 12666205. Source: UniProtKB

negative regulation of forebrain neuron differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of inner ear receptor cell differentiation

Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB

negative regulation of neuron differentiation

Inferred from mutant phenotype PubMed 10205173. Source: MGI

negative regulation of oligodendrocyte differentiation

Inferred from direct assay PubMed 12666205. Source: UniProtKB

negative regulation of pro-B cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of stem cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 17006542. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB

neural tube development

Inferred from genetic interaction PubMed 10804175. Source: MGI

neuronal stem cell maintenance

Inferred from mutant phenotype PubMed 11399758. Source: UniProtKB

oligodendrocyte development

Inferred from mutant phenotype PubMed 17006542. Source: MGI

positive regulation of BMP signaling pathway

Inferred from genetic interaction PubMed 18579678. Source: MGI

positive regulation of JAK-STAT cascade

Inferred from direct assay PubMed 15156153. Source: UniProtKB

positive regulation of Notch signaling pathway

Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of smooth muscle cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 19050759. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB

positive regulation of tyrosine phosphorylation of Stat3 protein

Inferred from direct assay PubMed 15156153. Source: UniProtKB

protein complex assembly

Inferred from direct assay PubMed 15156153. Source: UniProtKB

regulation of cell differentiation

Inferred from mutant phenotype PubMed 18048645. Source: MGI

regulation of myelination

Inferred from mutant phenotype PubMed 17006542. Source: MGI

regulation of neurogenesis

Inferred from genetic interaction PubMed 10804175PubMed 19050759. Source: MGI

smoothened signaling pathway

Inferred from direct assay PubMed 19124651. Source: MGI

specification of loop of Henle identity

Inferred from expression pattern PubMed 15821257. Source: UniProtKB

telencephalon development

Inferred from genetic interaction PubMed 18579678PubMed 20431123. Source: MGI

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 21259317. Source: UniProtKB

chromatin binding

Inferred from electronic annotation. Source: Ensembl

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 15156153PubMed 21259317. Source: UniProtKB

transcription factor binding

Non-traceable author statement PubMed 17611704. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 167167Transcription factor HES-5
PRO_0000127212

Regions

Domain16 – 7257bHLH
Domain88 – 11932Orange
Motif164 – 1674WRPW motif
Compositional bias14 – 3118Arg/Lys-rich (basic)
Compositional bias74 – 796Poly-Ala
Compositional bias122 – 14120Pro-rich
Compositional bias146 – 1527Poly-Ala

Experimental info

Sequence conflict221P → S in BAC37159. Ref.2
Sequence conflict941W → L in BAC37159. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P70120 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: CA0E697094F4E760

FASTA16718,425
        10         20         30         40         50         60 
MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH QPNSKLEKAD 

        70         80         90        100        110        120 
ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV QFLTLHAASD TQMKLLYHFQ 

       130        140        150        160 
RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA AAVSTSRQPA CGLWRPW 

« Hide

References

« Hide 'large scale' references
[1]"Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5. Identification of the neural precursor cell-specific promoter element."
Takebayashi K., Akazawa C., Nakanishi S., Kageyama R.
J. Biol. Chem. 270:1342-1349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye and Olfactory bulb.
[3]"BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets."
Tiberi L., van den Ameele J., Dimidschstein J., Piccirilli J., Gall D., Herpoel A., Bilheu A., Bonnefont J., Iacovino M., Kyba M., Bouschet T., Vanderhaeghen P.
Nat. Neurosci. 15:1627-1635(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS NEUROGENESIS REPRESSOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D32132 Genomic DNA. Translation: BAA06858.1.
AK078170 mRNA. Translation: BAC37159.1.
AK087508 mRNA. Translation: BAC39904.1.
CCDSCCDS19018.1.
PIRA55438.
RefSeqNP_034549.1. NM_010419.4.
UniGeneMm.137268.

3D structure databases

ProteinModelPortalP70120.
SMRP70120. Positions 12-71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200278. 1 interaction.

PTM databases

PhosphoSiteP70120.

Proteomic databases

PRIDEP70120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
GeneID15208.
KEGGmmu:15208.
UCSCuc008wck.1. mouse.

Organism-specific databases

CTD388585.
MGIMGI:104876. Hes5.

Phylogenomic databases

eggNOGNOG83691.
HOGENOMHOG000236346.
HOVERGENHBG005960.
InParanoidP70120.
KOK06055.
OMAHSANTDT.
OrthoDBEOG7GN2PQ.
PhylomeDBP70120.
TreeFamTF351373.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressP70120.
BgeeP70120.
CleanExMM_HES5.
GenevestigatorP70120.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR003650. Orange.
IPR018352. Orange_subgr.
[Graphical view]
PfamPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287769.
PROP70120.
SOURCESearch...

Entry information

Entry nameHES5_MOUSE
AccessionPrimary (citable) accession number: P70120
Secondary accession number(s): Q8BVI1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot