Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P70120

- HES5_MOUSE

UniProt

P70120 - HES5_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription factor HES-5

Gene

Hes5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.1 Publication

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. double-stranded DNA binding Source: Ensembl
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
  4. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. astrocyte differentiation Source: UniProtKB
  2. auditory receptor cell differentiation Source: MGI
  3. auditory receptor cell fate determination Source: MGI
  4. brain development Source: UniProtKB
  5. camera-type eye development Source: UniProtKB
  6. cartilage development Source: UniProtKB
  7. cell adhesion Source: MGI
  8. cell maturation Source: MGI
  9. central nervous system myelination Source: MGI
  10. comma-shaped body morphogenesis Source: UniProtKB
  11. forebrain radial glial cell differentiation Source: Ensembl
  12. glial cell fate commitment Source: MGI
  13. metanephric nephron tubule morphogenesis Source: UniProtKB
  14. negative regulation of astrocyte differentiation Source: UniProtKB
  15. negative regulation of forebrain neuron differentiation Source: Ensembl
  16. negative regulation of inner ear receptor cell differentiation Source: UniProtKB
  17. negative regulation of neuron differentiation Source: MGI
  18. negative regulation of oligodendrocyte differentiation Source: UniProtKB
  19. negative regulation of pro-B cell differentiation Source: UniProtKB
  20. negative regulation of stem cell differentiation Source: UniProtKB
  21. negative regulation of transcription, DNA-templated Source: UniProtKB
  22. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  23. neural tube development Source: MGI
  24. neuronal stem cell maintenance Source: UniProtKB
  25. Notch signaling pathway Source: UniProtKB
  26. oligodendrocyte development Source: MGI
  27. positive regulation of BMP signaling pathway Source: MGI
  28. positive regulation of cell proliferation Source: UniProtKB
  29. positive regulation of JAK-STAT cascade Source: UniProtKB
  30. positive regulation of Notch signaling pathway Source: UniProtKB
  31. positive regulation of smooth muscle cell proliferation Source: UniProtKB
  32. positive regulation of transcription, DNA-templated Source: UniProtKB
  33. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  34. positive regulation of tyrosine phosphorylation of Stat3 protein Source: UniProtKB
  35. protein complex assembly Source: UniProtKB
  36. regulation of cell differentiation Source: MGI
  37. regulation of myelination Source: MGI
  38. regulation of neurogenesis Source: MGI
  39. smoothened signaling pathway Source: MGI
  40. specification of loop of Henle identity Source: UniProtKB
  41. S-shaped body morphogenesis Source: UniProtKB
  42. telencephalon development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_196593. NOTCH2 intracellular domain regulates transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-5
Alternative name(s):
Hairy and enhancer of split 5
Gene namesi
Name:Hes5
Synonyms:Hes-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:104876. Hes5.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Transcription factor HES-5PRO_0000127212Add
BLAST

Proteomic databases

PRIDEiP70120.

PTM databases

PhosphoSiteiP70120.

Expressioni

Gene expression databases

BgeeiP70120.
CleanExiMM_HES5.
ExpressionAtlasiP70120. baseline and differential.
GenevestigatoriP70120.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

Protein-protein interaction databases

BioGridi200278. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP70120.
SMRiP70120. Positions 12-71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 7257bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini88 – 11932OrangePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi164 – 1674WRPW motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 3118Arg/Lys-rich (basic)Add
BLAST
Compositional biasi74 – 796Poly-Ala
Compositional biasi122 – 14120Pro-richAdd
BLAST
Compositional biasi146 – 1527Poly-Ala

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG83691.
HOGENOMiHOG000236346.
HOVERGENiHBG005960.
InParanoidiP70120.
KOiK06055.
OMAiHSANTDT.
OrthoDBiEOG7GN2PQ.
PhylomeDBiP70120.
TreeFamiTF351373.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange.
IPR018352. Orange_subgr.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70120 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH
60 70 80 90 100
QPNSKLEKAD ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV
110 120 130 140 150
QFLTLHAASD TQMKLLYHFQ RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA
160
AAVSTSRQPA CGLWRPW
Length:167
Mass (Da):18,425
Last modified:February 1, 1997 - v1
Checksum:iCA0E697094F4E760
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221P → S in BAC37159. (PubMed:16141072)Curated
Sequence conflicti94 – 941W → L in BAC37159. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D32132 Genomic DNA. Translation: BAA06858.1.
AK078170 mRNA. Translation: BAC37159.1.
AK087508 mRNA. Translation: BAC39904.1.
CCDSiCCDS19018.1.
PIRiA55438.
RefSeqiNP_034549.1. NM_010419.4.
UniGeneiMm.137268.

Genome annotation databases

EnsembliENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
GeneIDi15208.
KEGGimmu:15208.
UCSCiuc008wck.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D32132 Genomic DNA. Translation: BAA06858.1 .
AK078170 mRNA. Translation: BAC37159.1 .
AK087508 mRNA. Translation: BAC39904.1 .
CCDSi CCDS19018.1.
PIRi A55438.
RefSeqi NP_034549.1. NM_010419.4.
UniGenei Mm.137268.

3D structure databases

ProteinModelPortali P70120.
SMRi P70120. Positions 12-71.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200278. 1 interaction.

PTM databases

PhosphoSitei P70120.

Proteomic databases

PRIDEi P70120.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049621 ; ENSMUSP00000051118 ; ENSMUSG00000048001 .
GeneIDi 15208.
KEGGi mmu:15208.
UCSCi uc008wck.1. mouse.

Organism-specific databases

CTDi 388585.
MGIi MGI:104876. Hes5.

Phylogenomic databases

eggNOGi NOG83691.
HOGENOMi HOG000236346.
HOVERGENi HBG005960.
InParanoidi P70120.
KOi K06055.
OMAi HSANTDT.
OrthoDBi EOG7GN2PQ.
PhylomeDBi P70120.
TreeFami TF351373.

Enzyme and pathway databases

Reactomei REACT_196593. NOTCH2 intracellular domain regulates transcription.

Miscellaneous databases

NextBioi 287769.
PROi P70120.
SOURCEi Search...

Gene expression databases

Bgeei P70120.
CleanExi MM_HES5.
ExpressionAtlasi P70120. baseline and differential.
Genevestigatori P70120.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR003650. Orange.
IPR018352. Orange_subgr.
[Graphical view ]
Pfami PF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5. Identification of the neural precursor cell-specific promoter element."
    Takebayashi K., Akazawa C., Nakanishi S., Kageyama R.
    J. Biol. Chem. 270:1342-1349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye and Olfactory bulb.
  3. "BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets."
    Tiberi L., van den Ameele J., Dimidschstein J., Piccirilli J., Gall D., Herpoel A., Bilheu A., Bonnefont J., Iacovino M., Kyba M., Bouschet T., Vanderhaeghen P.
    Nat. Neurosci. 15:1627-1635(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS NEUROGENESIS REPRESSOR.

Entry informationi

Entry nameiHES5_MOUSE
AccessioniPrimary (citable) accession number: P70120
Secondary accession number(s): Q8BVI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3