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Protein

Transcription factor HES-5

Gene

Hes5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.1 Publication

GO - Molecular functioni

GO - Biological processi

  • astrocyte differentiation Source: UniProtKB
  • auditory receptor cell differentiation Source: MGI
  • auditory receptor cell fate determination Source: MGI
  • brain development Source: UniProtKB
  • camera-type eye development Source: UniProtKB
  • cartilage development Source: UniProtKB
  • cell adhesion Source: MGI
  • cell maturation Source: MGI
  • central nervous system myelination Source: MGI
  • comma-shaped body morphogenesis Source: UniProtKB
  • establishment of epithelial cell polarity Source: MGI
  • forebrain radial glial cell differentiation Source: Ensembl
  • glial cell fate commitment Source: MGI
  • inner ear receptor stereocilium organization Source: MGI
  • metanephric nephron tubule morphogenesis Source: UniProtKB
  • negative regulation of astrocyte differentiation Source: UniProtKB
  • negative regulation of forebrain neuron differentiation Source: Ensembl
  • negative regulation of inner ear receptor cell differentiation Source: UniProtKB
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of oligodendrocyte differentiation Source: UniProtKB
  • negative regulation of pro-B cell differentiation Source: UniProtKB
  • negative regulation of stem cell differentiation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neural tube development Source: MGI
  • neuronal stem cell population maintenance Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB
  • oligodendrocyte development Source: MGI
  • positive regulation of BMP signaling pathway Source: MGI
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of JAK-STAT cascade Source: UniProtKB
  • positive regulation of Notch signaling pathway Source: UniProtKB
  • positive regulation of smooth muscle cell proliferation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of tyrosine phosphorylation of Stat3 protein Source: UniProtKB
  • protein complex assembly Source: UniProtKB
  • regulation of auditory receptor cell differentiation Source: MGI
  • regulation of cell differentiation Source: MGI
  • regulation of epithelial cell proliferation Source: MGI
  • regulation of myelination Source: MGI
  • regulation of neurogenesis Source: MGI
  • smoothened signaling pathway Source: MGI
  • specification of loop of Henle identity Source: UniProtKB
  • S-shaped body morphogenesis Source: UniProtKB
  • telencephalon development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2197563. NOTCH2 intracellular domain regulates transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-5
Alternative name(s):
Hairy and enhancer of split 5
Gene namesi
Name:Hes5
Synonyms:Hes-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104876. Hes5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272121 – 167Transcription factor HES-5Add BLAST167

Proteomic databases

PaxDbiP70120.
PeptideAtlasiP70120.
PRIDEiP70120.

PTM databases

iPTMnetiP70120.
PhosphoSitePlusiP70120.

Expressioni

Gene expression databases

BgeeiENSMUSG00000048001.
CleanExiMM_HES5.
ExpressionAtlasiP70120. baseline and differential.
GenevisibleiP70120. MM.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi200278. 1 interactor.
STRINGi10090.ENSMUSP00000051118.

Structurei

3D structure databases

ProteinModelPortaliP70120.
SMRiP70120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 72bHLHPROSITE-ProRule annotationAdd BLAST57
Domaini88 – 119OrangePROSITE-ProRule annotationAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi164 – 167WRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 31Arg/Lys-rich (basic)Add BLAST18
Compositional biasi74 – 79Poly-Ala6
Compositional biasi122 – 141Pro-richAdd BLAST20
Compositional biasi146 – 152Poly-Ala7

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IWTV. Eukaryota.
ENOG41120DD. LUCA.
GeneTreeiENSGT00700000104168.
HOGENOMiHOG000236346.
HOVERGENiHBG005960.
InParanoidiP70120.
KOiK06055.
OMAiNRLRKPI.
OrthoDBiEOG091G0V3D.
PhylomeDBiP70120.
TreeFamiTF351373.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH
60 70 80 90 100
QPNSKLEKAD ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV
110 120 130 140 150
QFLTLHAASD TQMKLLYHFQ RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA
160
AAVSTSRQPA CGLWRPW
Length:167
Mass (Da):18,425
Last modified:February 1, 1997 - v1
Checksum:iCA0E697094F4E760
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22P → S in BAC37159 (PubMed:16141072).Curated1
Sequence conflicti94W → L in BAC37159 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32132 Genomic DNA. Translation: BAA06858.1.
AK078170 mRNA. Translation: BAC37159.1.
AK087508 mRNA. Translation: BAC39904.1.
CCDSiCCDS19018.1.
PIRiA55438.
RefSeqiNP_034549.1. NM_010419.4.
UniGeneiMm.137268.

Genome annotation databases

EnsembliENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
GeneIDi15208.
KEGGimmu:15208.
UCSCiuc008wck.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32132 Genomic DNA. Translation: BAA06858.1.
AK078170 mRNA. Translation: BAC37159.1.
AK087508 mRNA. Translation: BAC39904.1.
CCDSiCCDS19018.1.
PIRiA55438.
RefSeqiNP_034549.1. NM_010419.4.
UniGeneiMm.137268.

3D structure databases

ProteinModelPortaliP70120.
SMRiP70120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200278. 1 interactor.
STRINGi10090.ENSMUSP00000051118.

PTM databases

iPTMnetiP70120.
PhosphoSitePlusiP70120.

Proteomic databases

PaxDbiP70120.
PeptideAtlasiP70120.
PRIDEiP70120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
GeneIDi15208.
KEGGimmu:15208.
UCSCiuc008wck.1. mouse.

Organism-specific databases

CTDi388585.
MGIiMGI:104876. Hes5.

Phylogenomic databases

eggNOGiENOG410IWTV. Eukaryota.
ENOG41120DD. LUCA.
GeneTreeiENSGT00700000104168.
HOGENOMiHOG000236346.
HOVERGENiHBG005960.
InParanoidiP70120.
KOiK06055.
OMAiNRLRKPI.
OrthoDBiEOG091G0V3D.
PhylomeDBiP70120.
TreeFamiTF351373.

Enzyme and pathway databases

ReactomeiR-MMU-2197563. NOTCH2 intracellular domain regulates transcription.

Miscellaneous databases

PROiP70120.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048001.
CleanExiMM_HES5.
ExpressionAtlasiP70120. baseline and differential.
GenevisibleiP70120. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHES5_MOUSE
AccessioniPrimary (citable) accession number: P70120
Secondary accession number(s): Q8BVI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.