P70120 (HES5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor HES-5

Alternative name(s):
Hairy and enhancer of split 5

Gene names

Name:	Hes5
Synonyms:	Hes-5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional repressor of genes that require a bHLH protein for their transcription.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family By similarity.
Subcellular location	Nucleus.
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins By similarity.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from mutant phenotype PubMed 17331197. Source: UniProtKB S-shaped body morphogenesis Inferred from expression pattern PubMed 15821257. Source: UniProtKB astrocyte differentiation Inferred from direct assay PubMed 15156153. Source: UniProtKB auditory receptor cell fate determination Inferred from mutant phenotype PubMed 11425898. Source: MGI camera-type eye development Inferred from mutant phenotype PubMed 17331197. Source: UniProtKB cartilage development Inferred from mutant phenotype PubMed 17093926. Source: UniProtKB cell adhesion Inferred from genetic interaction PubMed 15496443. Source: MGI cell maturation Inferred from genetic interaction PubMed 15496443. Source: MGI central nervous system myelination Inferred from mutant phenotype PubMed 17006542. Source: MGI comma-shaped body morphogenesis Inferred from expression pattern PubMed 15821257. Source: UniProtKB forebrain radial glial cell differentiation Inferred from electronic annotation. Source: Compara glial cell fate commitment Inferred from mutant phenotype PubMed 10821751. Source: MGI metanephric nephron tubule morphogenesis Inferred from expression pattern PubMed 15465493. Source: UniProtKB negative regulation of astrocyte differentiation Inferred from direct assay PubMed 12666205. Source: UniProtKB negative regulation of forebrain neuron differentiation Inferred from electronic annotation. Source: Compara negative regulation of inner ear receptor cell differentiation Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB negative regulation of oligodendrocyte differentiation Inferred from direct assay PubMed 12666205. Source: UniProtKB negative regulation of pro-B cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of stem cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB neural tube development Inferred from genetic interaction PubMed 10804175. Source: MGI neuronal stem cell maintenance Inferred from mutant phenotype PubMed 11399758. Source: UniProtKB positive regulation of BMP signaling pathway Inferred from genetic interaction PubMed 18579678. Source: MGI positive regulation of Notch signaling pathway Inferred from mutant phenotype PubMed 12208538. Source: UniProtKB positive regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 19050759. Source: MGI positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from direct assay PubMed 15156153. Source: UniProtKB protein complex assembly Inferred from direct assay PubMed 15156153. Source: UniProtKB regulation of myelination Inferred from mutant phenotype PubMed 17006542. Source: MGI smoothened signaling pathway Inferred from direct assay PubMed 19124651. Source: MGI specification of loop of Henle identity Inferred from expression pattern PubMed 15821257. Source: UniProtKB telencephalon development Inferred from genetic interaction PubMed 18579678PubMed 20431123. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 21259317. Source: UniProtKB chromatin binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara transcription factor binding Non-traceable author statement PubMed 17611704. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Transcription factor HES-5		PRO_0000127212
Regions
	Domain	16 – 72	57	bHLH
	Domain	88 – 119	32	Orange
	Motif	164 – 167	4	WRPW motif
	Compositional bias	14 – 31	18	Arg/Lys-rich (basic)
	Compositional bias	74 – 79	6	Poly-Ala
	Compositional bias	122 – 141	20	Pro-rich
	Compositional bias	146 – 152	7	Poly-Ala
Experimental info
	Sequence conflict	22	1	P → S in BAC37159. Ref.2
	Sequence conflict	94	1	W → L in BAC37159. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P70120 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: CA0E697094F4E760	FASTA	167	18,425
	10 20 30 40 50 60 MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH QPNSKLEKAD 70 80 90 100 110 120 ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV QFLTLHAASD TQMKLLYHFQ 130 140 150 160 RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA AAVSTSRQPA CGLWRPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5. Identification of the neural precursor cell-specific promoter element." Takebayashi K., Akazawa C., Nakanishi S., Kageyama R. J. Biol. Chem. 270:1342-1349(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye and Olfactory bulb.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D32132 Genomic DNA. Translation: BAA06858.1. AK078170 mRNA. Translation: BAC37159.1. AK087508 mRNA. Translation: BAC39904.1.
IPI	IPI00136148.
PIR	A55438.
RefSeq	NP_034549.1. NM_010419.4.
UniGene	Mm.137268.
3D structure databases
ProteinModelPortal	P70120.
SMR	P70120. Positions 29-79.
ModBase	Search...
PTM databases
PhosphoSite	P70120.
Proteomic databases
PRIDE	P70120.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
GeneID	15208.
KEGG	mmu:15208.
UCSC	uc008wck.1. mouse.
Organism-specific databases
CTD	388585.
MGI	MGI:104876. Hes5.
Phylogenomic databases
eggNOG	NOG83691.
HOGENOM	HOG000236346.
HOVERGEN	HBG005960.
InParanoid	P70120.
KO	K06055.
OMA	QFLTLHS.
OrthoDB	EOG495ZT5.
Gene expression databases
ArrayExpress	P70120.
Bgee	P70120.
CleanEx	MM_HES5.
Genevestigator	P70120.
GermOnline	ENSMUSG00000048001. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. IPR003650. Orange. IPR018352. Orange_subgr. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	287769.
SOURCE	Search...

Entry information

Entry name

HES5_MOUSE

Accession

Primary (citable) accession number: P70120
Secondary accession number(s): Q8BVI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program