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P70120

- HES5_MOUSE

UniProt

P70120 - HES5_MOUSE

Protein

Transcription factor HES-5

Gene

Hes5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.1 Publication

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. double-stranded DNA binding Source: Ensembl
    3. protein binding Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
    5. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. astrocyte differentiation Source: UniProtKB
    2. auditory receptor cell differentiation Source: MGI
    3. auditory receptor cell fate determination Source: MGI
    4. brain development Source: UniProtKB
    5. camera-type eye development Source: UniProtKB
    6. cartilage development Source: UniProtKB
    7. cell adhesion Source: MGI
    8. cell maturation Source: MGI
    9. central nervous system myelination Source: MGI
    10. comma-shaped body morphogenesis Source: UniProtKB
    11. forebrain radial glial cell differentiation Source: Ensembl
    12. glial cell fate commitment Source: MGI
    13. metanephric nephron tubule morphogenesis Source: UniProtKB
    14. negative regulation of astrocyte differentiation Source: UniProtKB
    15. negative regulation of forebrain neuron differentiation Source: Ensembl
    16. negative regulation of inner ear receptor cell differentiation Source: UniProtKB
    17. negative regulation of neuron differentiation Source: MGI
    18. negative regulation of oligodendrocyte differentiation Source: UniProtKB
    19. negative regulation of pro-B cell differentiation Source: UniProtKB
    20. negative regulation of stem cell differentiation Source: UniProtKB
    21. negative regulation of transcription, DNA-templated Source: UniProtKB
    22. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    23. neural tube development Source: MGI
    24. neuronal stem cell maintenance Source: UniProtKB
    25. Notch signaling pathway Source: UniProtKB
    26. oligodendrocyte development Source: MGI
    27. positive regulation of BMP signaling pathway Source: MGI
    28. positive regulation of cell proliferation Source: UniProtKB
    29. positive regulation of JAK-STAT cascade Source: UniProtKB
    30. positive regulation of Notch signaling pathway Source: UniProtKB
    31. positive regulation of smooth muscle cell proliferation Source: UniProtKB
    32. positive regulation of transcription, DNA-templated Source: UniProtKB
    33. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    34. positive regulation of tyrosine phosphorylation of Stat3 protein Source: UniProtKB
    35. protein complex assembly Source: UniProtKB
    36. regulation of cell differentiation Source: MGI
    37. regulation of myelination Source: MGI
    38. regulation of neurogenesis Source: MGI
    39. smoothened signaling pathway Source: MGI
    40. specification of loop of Henle identity Source: UniProtKB
    41. S-shaped body morphogenesis Source: UniProtKB
    42. telencephalon development Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_196593. NOTCH2 intracellular domain regulates transcription.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor HES-5
    Alternative name(s):
    Hairy and enhancer of split 5
    Gene namesi
    Name:Hes5
    Synonyms:Hes-5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:104876. Hes5.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Transcription factor HES-5PRO_0000127212Add
    BLAST

    Proteomic databases

    PRIDEiP70120.

    PTM databases

    PhosphoSiteiP70120.

    Expressioni

    Gene expression databases

    ArrayExpressiP70120.
    BgeeiP70120.
    CleanExiMM_HES5.
    GenevestigatoriP70120.

    Interactioni

    Subunit structurei

    Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

    Protein-protein interaction databases

    BioGridi200278. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP70120.
    SMRiP70120. Positions 12-71.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 7257bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini88 – 11932OrangePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi164 – 1674WRPW motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 3118Arg/Lys-rich (basic)Add
    BLAST
    Compositional biasi74 – 796Poly-Ala
    Compositional biasi122 – 14120Pro-richAdd
    BLAST
    Compositional biasi146 – 1527Poly-Ala

    Domaini

    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 1 Orange domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG83691.
    HOGENOMiHOG000236346.
    HOVERGENiHBG005960.
    InParanoidiP70120.
    KOiK06055.
    OMAiHSANTDT.
    OrthoDBiEOG7GN2PQ.
    PhylomeDBiP70120.
    TreeFamiTF351373.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR003650. Orange.
    IPR018352. Orange_subgr.
    [Graphical view]
    PfamiPF07527. Hairy_orange. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00511. ORANGE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    PS51054. ORANGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P70120-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH    50
    QPNSKLEKAD ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV 100
    QFLTLHAASD TQMKLLYHFQ RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA 150
    AAVSTSRQPA CGLWRPW 167
    Length:167
    Mass (Da):18,425
    Last modified:February 1, 1997 - v1
    Checksum:iCA0E697094F4E760
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221P → S in BAC37159. (PubMed:16141072)Curated
    Sequence conflicti94 – 941W → L in BAC37159. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32132 Genomic DNA. Translation: BAA06858.1.
    AK078170 mRNA. Translation: BAC37159.1.
    AK087508 mRNA. Translation: BAC39904.1.
    CCDSiCCDS19018.1.
    PIRiA55438.
    RefSeqiNP_034549.1. NM_010419.4.
    UniGeneiMm.137268.

    Genome annotation databases

    EnsembliENSMUST00000049621; ENSMUSP00000051118; ENSMUSG00000048001.
    GeneIDi15208.
    KEGGimmu:15208.
    UCSCiuc008wck.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32132 Genomic DNA. Translation: BAA06858.1 .
    AK078170 mRNA. Translation: BAC37159.1 .
    AK087508 mRNA. Translation: BAC39904.1 .
    CCDSi CCDS19018.1.
    PIRi A55438.
    RefSeqi NP_034549.1. NM_010419.4.
    UniGenei Mm.137268.

    3D structure databases

    ProteinModelPortali P70120.
    SMRi P70120. Positions 12-71.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200278. 1 interaction.

    PTM databases

    PhosphoSitei P70120.

    Proteomic databases

    PRIDEi P70120.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049621 ; ENSMUSP00000051118 ; ENSMUSG00000048001 .
    GeneIDi 15208.
    KEGGi mmu:15208.
    UCSCi uc008wck.1. mouse.

    Organism-specific databases

    CTDi 388585.
    MGIi MGI:104876. Hes5.

    Phylogenomic databases

    eggNOGi NOG83691.
    HOGENOMi HOG000236346.
    HOVERGENi HBG005960.
    InParanoidi P70120.
    KOi K06055.
    OMAi HSANTDT.
    OrthoDBi EOG7GN2PQ.
    PhylomeDBi P70120.
    TreeFami TF351373.

    Enzyme and pathway databases

    Reactomei REACT_196593. NOTCH2 intracellular domain regulates transcription.

    Miscellaneous databases

    NextBioi 287769.
    PROi P70120.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P70120.
    Bgeei P70120.
    CleanExi MM_HES5.
    Genevestigatori P70120.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR003650. Orange.
    IPR018352. Orange_subgr.
    [Graphical view ]
    Pfami PF07527. Hairy_orange. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00511. ORANGE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    PS51054. ORANGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5. Identification of the neural precursor cell-specific promoter element."
      Takebayashi K., Akazawa C., Nakanishi S., Kageyama R.
      J. Biol. Chem. 270:1342-1349(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye and Olfactory bulb.
    3. "BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets."
      Tiberi L., van den Ameele J., Dimidschstein J., Piccirilli J., Gall D., Herpoel A., Bilheu A., Bonnefont J., Iacovino M., Kyba M., Bouschet T., Vanderhaeghen P.
      Nat. Neurosci. 15:1627-1635(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS NEUROGENESIS REPRESSOR.

    Entry informationi

    Entry nameiHES5_MOUSE
    AccessioniPrimary (citable) accession number: P70120
    Secondary accession number(s): Q8BVI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3