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Protein

Heat-stable enterotoxin receptor

Gene

GUCY2C

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat-stable enterotoxin receptor (EC:4.6.1.2)
Short name:
STA receptor
Alternative name(s):
Guanylyl cyclase C
Short name:
GC-C
Intestinal guanylate cyclase
Gene namesi
Name:GUCY2C
Synonyms:GUC2C
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 433410ExtracellularSequence analysisAdd
BLAST
Transmembranei434 – 45724HelicalSequence analysisAdd
BLAST
Topological domaini458 – 1076619CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 10761053Heat-stable enterotoxin receptorPRO_0000012375Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence analysis
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence analysis
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylation at Asn-62 is required for interaction with VIP36 while glycosylation at Asn-348 and Asn-405 modulates ligand-mediated GC-C activation.By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000018324.

Structurei

3D structure databases

ProteinModelPortaliP70106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini492 – 752261Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini827 – 957131Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000112833.
HOVERGENiHBG106967.
InParanoidiP70106.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPLLGLVV WSLLLQLLQP GLAFWNSQIS QNCHNGSYEI TVLMMNNYAF
60 70 80 90 100
QESLESLKTA VNKGLDIVKQ RLQEAALYVT VNATFIHSDG LIHKSGDCRS
110 120 130 140 150
STCEGLDLLR EIARQKRMGC ALMGPSCTYS TYQMYLDTEL NYPMISAGSF
160 170 180 190 200
GLSCDHKETL TRMMSPARKL MYFLVDFWKA SNLPFKSFSW NTSYVFKNGT
210 220 230 240 250
ESEHCFWYIN ALEAGVSYFS QVLGFKEMLR GNEELQKILK DPNRRSNVIV
260 270 280 290 300
MCGTPQTMES LKIDWTATED TVIILVDLFN NYYLEENATA PDYMKNVLVL
310 320 330 340 350
TLPPGNSTIN TSLSKESLQE FSDFALAYLD GILLFGHMLK TFLRNGENTT
360 370 380 390 400
AHKFAHAFRN LTFEGSTGPV TLDDSGDIDN TMVLLYTSVD TKKFKPLLFY
410 420 430 440 450
DTRINQTTPI DTHPTFIWKN HRLPHDIPGL GPHILLIAVC TLAGVVVLIL
460 470 480 490 500
LIALLVLRKY KKDNELRQKK WSHIPPEKIL PLETNEANHV SLKIDDDKKR
510 520 530 540 550
DTVQRLRQCK YDPKRAILKD LKYSDGNFSE KQKIELDKLL PSDFYSLTKF
560 570 580 590 600
YGTVKLDTRI FGVIEYCERG SLREVLNETI SYPDGTIMGW EFKISVLYDI
610 620 630 640 650
AKGMSYLHSS KIEVHGRLKS TNCVVDSRMV VKITDFGYNS ILPPKKDLWT
660 670 680 690 700
APEHLRQAST SQKGDVYSFG IIAQEIIMRR ETFYTLSCRD QKEKIFRVEH
710 720 730 740 750
PDGLKPFRPD LFLETAEEKE LEVFLLVKNC WEEDPEKRPD FKKIENTLAK
760 770 780 790 800
IFGLFHDQKN ESYMDTLIRR LQLYSRNLEH LVEERTQLYK AERDRADRLN
810 820 830 840 850
FMLLPRPVVQ SLKEKGIVEP ELYEEVTVYF SDIVGFTTIC KYSTPMEVVD
860 870 880 890 900
MLNDLYKSFD QIVDHHDVHK VETIGDAYVV ASGLPTRNGN RHAIDISKMA
910 920 930 940 950
LDILSFIGTF ELEHLPGLPV WIRIGVHSGP CAAGVVGIKI PRYCLFGDTV
960 970 980 990 1000
NTASRMESTG LPLRIHMSSS TIAILKRVQC QFLYEMRGET YLKGKGTETT
1010 1020 1030 1040 1050
YCLTGMKDQE YNLPTPPTVE NQQRLQAEFS DMITNSLQKR QATGIKSRKP
1060 1070
ARVASYKKGT LEYLQLNTTD QDSTYF
Length:1,076
Mass (Da):123,120
Last modified:February 1, 1997 - v1
Checksum:i9B53F16E05E80EB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74734 mRNA. Translation: CAA98989.1.
RefSeqiNP_001166430.1. NM_001172959.2.

Genome annotation databases

GeneIDi100135536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74734 mRNA. Translation: CAA98989.1.
RefSeqiNP_001166430.1. NM_001172959.2.

3D structure databases

ProteinModelPortaliP70106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000018324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135536.

Organism-specific databases

CTDi2984.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000112833.
HOVERGENiHBG106967.
InParanoidiP70106.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUC2C_CAVPO
AccessioniPrimary (citable) accession number: P70106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 11, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.