Reviewed, UniProtKB/Swiss-Prot P70091 (CP19A_ORENI)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 19A1
    EC=1.14.14.1
Alternative name(s):
    CYPXIX
    Aromatase
    Estrogen synthetase
    P-450AROM

Gene names

Name:	cyp19a1
Synonyms:	cyp19

Organism

Oreochromis niloticus (Nile tilapia) (Tilapia nilotica)

Taxonomic identifier

8128 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Labroidei › Cichlidae › African cichlids › Pseudocrenilabrinae › Tilapiini › Oreochromis

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Cytochrome P450 19A1		PRO_0000051971
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70091-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EA62E690CF8C06F6	FASTA	522	59,428
	10 20 30 40 50 60 MDLISACEQA MNPVGLDAVV ARSLCDLKCH PIDGISMATR TLILLVCLLL VAWSHTDKKI 70 80 90 100 110 120 VPGPSFCLGL GPLLSYLRFI WTGIGTASNY YNNKYGDIVR VWINGEETLI LSRSSAVHHV 130 140 150 160 170 180 LKNGNYTSRF GSIQGLSYLG MNERGIIFNN NVTLWKKIRT YFAKALTGPN LQQTVDVCVS 190 200 210 220 230 240 SIQAHLDHLD SLGHVDVLNL LRCTVLDISN RLFLNVPLNE KELMLKIQKY FHTWQDVLIK 250 260 270 280 290 300 PDIYFKFRWI HHRHKTATQE LQDAIKRLVD QKRKNMEQAD KLDNINFTAE LIFAQNHGEL 310 320 330 340 350 360 SAENVTQCVL EMVIAAPDTL SLSLFFMLLL LKQNPHVEPQ LLQEIDAVVG ERQLQNQDLH 370 380 390 400 410 420 KLQVMESFIY ECLSFHPVVD FTMRRALSDD IIEGYRISKG TNIILNTGRM HRTEFFLKGN 430 440 450 460 470 480 QFNLEHFENN VPRPPTFQPF GSGPRACIGK HMAMVMMKSI LVTLLSQYSV CTHEGPILDC 490 500 510 520 LPQTNNLSQQ PVEHQQAETE HLHMRFLPRQ GSSCQTLKDP NL

References

[1]

"Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis." Chang X.T., Kobayashi T., Kajiura H., Nakamura M., Nagahama Y. J. Mol. Endocrinol. 18:57-66(1997) [PubMed: 9061607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U72071 mRNA. Translation: AAB16814.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P70091.
Enzyme and pathway databases
BRENDA	1.14.14.1. 39198.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP19A_ORENI

Accession

Primary (citable) accession number: P70091

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	October 13, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)