P70085 (CP17A_ORYLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase
EC=1.14.99.9
EC=4.1.2.30

Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17

Gene names

Name:	cyp17a1
Synonyms:	cyp17

Organism

Oryzias latipes (Medaka fish) (Japanese ricefish) [Reference proteome]

Taxonomic identifier

8090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Smegmamorpha › Atherinomorpha › Beloniformes › Adrianichthyoidei › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.
Catalytic activity	A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O. 17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Ligand	Heme Iron Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform C17L (identifier: P70085-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C17S (identifier: P70085-2)

The sequence of this isoform differs from the canonical sequence as follows:
     230-258: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051946
Sites
	Metal binding	451	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	230 – 258	29	Missing in isoform C17S.		VSP_000615

Sequences

	Sequence		Length	Mass (Da)
	Isoform C17L [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7A67509437ED6C6E	FASTA	517	57,524
	10 20 30 40 50 60 MAWFLCLSVL VVLVLALAAL LWRVRTRDRP QEAPSLPYLP VLGSLLSLRS PHPPHVLFKE 70 80 90 100 110 120 LQQKYGQTYS LKMGSHQVII VNHHAHAREV LLKRGRTFAG RPRTVTTDVL TRDGKDIAFG 130 140 150 160 170 180 DYSATWRFHR KIVHGALCMF GEGSASLQRI ICTEAQSLCS TLSEAAATGL ALDLSPELTR 190 200 210 220 230 240 AVTNVICSLC FNSSYSRGDP EFEAMLRYSQ GIVDTVAKDS LVDIFPWLQI FPNKDLRLLK 250 260 270 280 290 300 QCVAVRDQLL QKKFEEHKSD YSDHVQRDLL DALLRAKRSA ENNNTAAEFS AEAVGLSDDH 310 320 330 340 350 360 LLMTVGDIFG AGVETTTTVL KWAITYLIHY PEVQKQIQEE LDRKVGVDRP PQLSDRGSLP 370 380 390 400 410 420 FLEATIREVL RIRPVAPLLI PHVALSDTSL GDFTVRKGTR VVINLWSLHH DEKEWTNPDL 430 440 450 460 470 480 FNPGRFLSAD GSSLTLPSSS YLPFGAGLRV CLGEALAKME LFLFLSWILQ RFTLSVPPSQ 490 500 510 SLPSLEGKFG VVLQPVKYAV KATPRPGCHS GLFPANP
	Isoform C17S [UniParc]. Checksum: 640239B9AB8B624B	FASTA	488	54,004
	10 20 30 40 50 60 MAWFLCLSVL VVLVLALAAL LWRVRTRDRP QEAPSLPYLP VLGSLLSLRS PHPPHVLFKE 70 80 90 100 110 120 LQQKYGQTYS LKMGSHQVII VNHHAHAREV LLKRGRTFAG RPRTVTTDVL TRDGKDIAFG 130 140 150 160 170 180 DYSATWRFHR KIVHGALCMF GEGSASLQRI ICTEAQSLCS TLSEAAATGL ALDLSPELTR 190 200 210 220 230 240 AVTNVICSLC FNSSYSRGDP EFEAMLRYSQ GIVDTVAKDS LVDIFPWLQS DYSDHVQRDL 250 260 270 280 290 300 LDALLRAKRS AENNNTAAEF SAEAVGLSDD HLLMTVGDIF GAGVETTTTV LKWAITYLIH 310 320 330 340 350 360 YPEVQKQIQE ELDRKVGVDR PPQLSDRGSL PFLEATIREV LRIRPVAPLL IPHVALSDTS 370 380 390 400 410 420 LGDFTVRKGT RVVINLWSLH HDEKEWTNPD LFNPGRFLSA DGSSLTLPSS SYLPFGAGLR 430 440 450 460 470 480 VCLGEALAKM ELFLFLSWIL QRFTLSVPPS QSLPSLEGKF GVVLQPVKYA VKATPRPGCH SGLFPANP

References

[1]

"Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P-450 aromatase." Fukada S., Tanaka M., Matsuyama M., Kobayashi D., Nagahama Y. Mol. Reprod. Dev. 45:285-290(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS C17L AND C17S). Strain: Orange-red. Tissue: Ovarian follicle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87121 mRNA. Translation: BAA13252.1. D87122 mRNA. Translation: BAA13253.1.
RefSeq	NP_001098564.1. NM_001105094.1.
UniGene	Ola.85.
3D structure databases
ProteinModelPortal	P70085.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSORLT00000023962; ENSORLP00000023961; ENSORLG00000019226.
GeneID	100125816.
KEGG	ola:100125816.
Organism-specific databases
CTD	100313515.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00690000101630.
HOGENOM	HOG000036991.
HOVERGEN	HBG106944.
InParanoid	P70085.
KO	K00512.
Enzyme and pathway databases
UniPathway	UPA00062.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_ORYLA

Accession

Primary (citable) accession number: P70085
Secondary accession number(s): P70086

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program