Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P70085

- CP17A_ORYLA

UniProt

P70085 - CP17A_ORYLA

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

cyp17a1

Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi451 – 4511Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:cyp17a1
    Synonyms:cyp17
    OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)
    Taxonomic identifieri8090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
    ProteomesiUP000001038: Unplaced

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 517517Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051946Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi8090.ENSORLP00000023959.

    Structurei

    3D structure databases

    ProteinModelPortaliP70085.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiP70085.
    KOiK00512.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform C17L (identifier: P70085-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAWFLCLSVL VVLVLALAAL LWRVRTRDRP QEAPSLPYLP VLGSLLSLRS    50
    PHPPHVLFKE LQQKYGQTYS LKMGSHQVII VNHHAHAREV LLKRGRTFAG 100
    RPRTVTTDVL TRDGKDIAFG DYSATWRFHR KIVHGALCMF GEGSASLQRI 150
    ICTEAQSLCS TLSEAAATGL ALDLSPELTR AVTNVICSLC FNSSYSRGDP 200
    EFEAMLRYSQ GIVDTVAKDS LVDIFPWLQI FPNKDLRLLK QCVAVRDQLL 250
    QKKFEEHKSD YSDHVQRDLL DALLRAKRSA ENNNTAAEFS AEAVGLSDDH 300
    LLMTVGDIFG AGVETTTTVL KWAITYLIHY PEVQKQIQEE LDRKVGVDRP 350
    PQLSDRGSLP FLEATIREVL RIRPVAPLLI PHVALSDTSL GDFTVRKGTR 400
    VVINLWSLHH DEKEWTNPDL FNPGRFLSAD GSSLTLPSSS YLPFGAGLRV 450
    CLGEALAKME LFLFLSWILQ RFTLSVPPSQ SLPSLEGKFG VVLQPVKYAV 500
    KATPRPGCHS GLFPANP 517
    Length:517
    Mass (Da):57,524
    Last modified:February 1, 1997 - v1
    Checksum:i7A67509437ED6C6E
    GO
    Isoform C17S (identifier: P70085-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         230-258: Missing.

    Show »
    Length:488
    Mass (Da):54,004
    Checksum:i640239B9AB8B624B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei230 – 25829Missing in isoform C17S. 1 PublicationVSP_000615Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87121 mRNA. Translation: BAA13252.1.
    D87122 mRNA. Translation: BAA13253.1.
    RefSeqiNP_001098564.1. NM_001105094.1. [P70085-1]
    UniGeneiOla.85.

    Genome annotation databases

    EnsembliENSORLT00000023962; ENSORLP00000023961; ENSORLG00000019226. [P70085-2]
    GeneIDi100125816.
    KEGGiola:100125816.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87121 mRNA. Translation: BAA13252.1 .
    D87122 mRNA. Translation: BAA13253.1 .
    RefSeqi NP_001098564.1. NM_001105094.1. [P70085-1 ]
    UniGenei Ola.85.

    3D structure databases

    ProteinModelPortali P70085.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8090.ENSORLP00000023959.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSORLT00000023962 ; ENSORLP00000023961 ; ENSORLG00000019226 . [P70085-2 ]
    GeneIDi 100125816.
    KEGGi ola:100125816.

    Organism-specific databases

    CTDi 100313515.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi P70085.
    KOi K00512.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P-450 aromatase."
      Fukada S., Tanaka M., Matsuyama M., Kobayashi D., Nagahama Y.
      Mol. Reprod. Dev. 45:285-290(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS C17L AND C17S).
      Strain: Orange-red.
      Tissue: Ovarian follicle.

    Entry informationi

    Entry nameiCP17A_ORYLA
    AccessioniPrimary (citable) accession number: P70085
    Secondary accession number(s): P70086
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3