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P70085 (CP17A_ORYLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:cyp17a1
Synonyms:cyp17
OrganismOryzias latipes (Medaka fish) (Japanese ricefish) [Reference proteome]
Taxonomic identifier8090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   LigandHeme
Iron
Metal-binding
   Molecular functionLyase
Monooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsteroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function17-alpha-hydroxyprogesterone aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 17-alpha-monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform C17L (identifier: P70085-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform C17S (identifier: P70085-2)

The sequence of this isoform differs from the canonical sequence as follows:
     230-258: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 517517Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051946

Sites

Metal binding4511Iron (heme axial ligand) By similarity

Natural variations

Alternative sequence230 – 25829Missing in isoform C17S.
VSP_000615

Sequences

Sequence LengthMass (Da)Tools
Isoform C17L [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 7A67509437ED6C6E

FASTA51757,524
        10         20         30         40         50         60 
MAWFLCLSVL VVLVLALAAL LWRVRTRDRP QEAPSLPYLP VLGSLLSLRS PHPPHVLFKE 

        70         80         90        100        110        120 
LQQKYGQTYS LKMGSHQVII VNHHAHAREV LLKRGRTFAG RPRTVTTDVL TRDGKDIAFG 

       130        140        150        160        170        180 
DYSATWRFHR KIVHGALCMF GEGSASLQRI ICTEAQSLCS TLSEAAATGL ALDLSPELTR 

       190        200        210        220        230        240 
AVTNVICSLC FNSSYSRGDP EFEAMLRYSQ GIVDTVAKDS LVDIFPWLQI FPNKDLRLLK 

       250        260        270        280        290        300 
QCVAVRDQLL QKKFEEHKSD YSDHVQRDLL DALLRAKRSA ENNNTAAEFS AEAVGLSDDH 

       310        320        330        340        350        360 
LLMTVGDIFG AGVETTTTVL KWAITYLIHY PEVQKQIQEE LDRKVGVDRP PQLSDRGSLP 

       370        380        390        400        410        420 
FLEATIREVL RIRPVAPLLI PHVALSDTSL GDFTVRKGTR VVINLWSLHH DEKEWTNPDL 

       430        440        450        460        470        480 
FNPGRFLSAD GSSLTLPSSS YLPFGAGLRV CLGEALAKME LFLFLSWILQ RFTLSVPPSQ 

       490        500        510 
SLPSLEGKFG VVLQPVKYAV KATPRPGCHS GLFPANP 

« Hide

Isoform C17S [UniParc].

Checksum: 640239B9AB8B624B
Show »

FASTA48854,004

References

[1]"Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P-450 aromatase."
Fukada S., Tanaka M., Matsuyama M., Kobayashi D., Nagahama Y.
Mol. Reprod. Dev. 45:285-290(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS C17L AND C17S).
Strain: Orange-red.
Tissue: Ovarian follicle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87121 mRNA. Translation: BAA13252.1.
D87122 mRNA. Translation: BAA13253.1.
RefSeqNP_001098564.1. NM_001105094.1. [P70085-1]
UniGeneOla.85.

3D structure databases

ProteinModelPortalP70085.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8090.ENSORLP00000023959.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSORLT00000023962; ENSORLP00000023961; ENSORLG00000019226. [P70085-2]
GeneID100125816.
KEGGola:100125816.

Organism-specific databases

CTD100313515.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117317.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidP70085.
KOK00512.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP17A_ORYLA
AccessionPrimary (citable) accession number: P70085
Secondary accession number(s): P70086
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways