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Protein

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Gene

atp2a1

Organism
Makaira nigricans (Atlantic blue marlin)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Inhibited by sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (By similarity). Enhanced by DWORF; DWORF increases activity by displacing sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi305Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi307Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi309Calcium 2By similarity1
Active sitei3514-aspartylphosphate intermediateBy similarity1
Metal bindingi700MagnesiumBy similarity1
Metal bindingi704MagnesiumBy similarity1
Metal bindingi765Calcium 1By similarity1
Metal bindingi768Calcium 1By similarity1
Metal bindingi793Calcium 2By similarity1
Metal bindingi796Calcium 1By similarity1
Metal bindingi797Calcium 1By similarity1
Metal bindingi797Calcium 2By similarity1
Metal bindingi905Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC:3.6.3.8)
Short name:
SERCA1
Short name:
SR Ca(2+)-ATPase 1
Alternative name(s):
Calcium pump 1
Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene namesi
Name:atp2a1
OrganismiMakaira nigricans (Atlantic blue marlin)
Taxonomic identifieri13604 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataCarangariaIstiophoriformesMakaira

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicBy similarityAdd BLAST48
Transmembranei49 – 69Helical; Name=1By similarityAdd BLAST21
Topological domaini70 – 89LumenalBy similarityAdd BLAST20
Transmembranei90 – 110Helical; Name=2By similarityAdd BLAST21
Topological domaini111 – 253CytoplasmicBy similarityAdd BLAST143
Transmembranei254 – 273Helical; Name=3By similarityAdd BLAST20
Topological domaini274 – 295LumenalBy similarityAdd BLAST22
Transmembranei296 – 313Helical; Name=4By similarityAdd BLAST18
Topological domaini314 – 754CytoplasmicBy similarityAdd BLAST441
Transmembranei755 – 774Helical; Name=5By similarityAdd BLAST20
Topological domaini775 – 784LumenalBy similarity10
Transmembranei785 – 805Helical; Name=6By similarityAdd BLAST21
Topological domaini806 – 825CytoplasmicBy similarityAdd BLAST20
Transmembranei826 – 848Helical; Name=7By similarityAdd BLAST23
Topological domaini849 – 894LumenalBy similarityAdd BLAST46
Transmembranei895 – 914Helical; Name=8By similarityAdd BLAST20
Topological domaini915 – 927CytoplasmicBy similarityAdd BLAST13
Transmembranei928 – 946Helical; Name=9By similarityAdd BLAST19
Topological domaini947 – 961LumenalBy similarityAdd BLAST15
Transmembranei962 – 982Helical; Name=10By similarityAdd BLAST21
Topological domaini983 – 996CytoplasmicBy similarityAdd BLAST14

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000461921 – 996Sarcoplasmic/endoplasmic reticulum calcium ATPase 1Add BLAST996

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi873 ↔ 885By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiP70083.

Interactioni

Subunit structurei

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP70083.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni370 – 400Interaction with phospholamban 1By similarityAdd BLAST31
Regioni785 – 805Interaction with phospholamban 2By similarityAdd BLAST21

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105648.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SERCA1B (identifier: P70083-1) [UniParc]FASTAAdd to basket
Also known as: ATP2A1B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENAHTKSPA ECLSYFGVNE HTGLSPDQFK KNLDKFGYNE LPAEEGKSIW
60 70 80 90 100
DLIVEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA
110 120 130 140 150
NAIVGVWQER NAEDAIEALK EYEPEMGKVY RSDRKSVQRI KAREIVPGDI
160 170 180 190 200
VEVSVGDKVP ADIRIVSIKS TTLRVDQSIL TGESVSVIKH TESVPDPRAV
210 220 230 240 250
NQDKKNMLFS GTNIAAGKAI GVAIATGVST EIGKIRDQMA ATEQEKTPLQ
260 270 280 290 300
AKLDEFGEQL SKVISLICVA VWAINIGHFN DPVHGGSWIR GAVYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MCVTKMFIVK SVDGDHVDLN AFDISGSKYT PEGEVSHGGS
410 420 430 440 450
KTNCSAYDGL VELATICALC NDSSLDYNES KKIYEKVGEA TETALCCLVE
460 470 480 490 500
KMNVFNSNVK NLSRIERANA CCTVIKQLMK KNFTLEFSRD RKSMSVYCTP
510 520 530 540 550
AKGDGGAKMF VKGAPEGVID RCAYVRVGTT RVPLTSAIKE KIMAVIRDWG
560 570 580 590 600
TGRDTLRCLA LATRDTPLKV EEMNLEDSTK FADYETDMTF VGCVGMLDPP
610 620 630 640 650
RKEVTGSIEL CRDAGIRVIM ITGDNKGTAI AICRRIGIFK EDEDVSNKAY
660 670 680 690 700
TGREFDDLPS QDQAEAVRRA CCFARVEPSH KSKIVEFLQG NDDITAMTGD
710 720 730 740 750
GVNDAPALKK AEIGIAMGSG TAVAKSASEM VLADDNFSSI VAAVEEGRAI
760 770 780 790 800
YNNMKQFIRY LISSNVGEVV CIFLTAALGL PEALIPVQLL WVNLVTDGLP
810 820 830 840 850
ATALGFNPPD LDIMGKPPRS PKEPLISGWL FFRYMAIGGY VGAATVGGAA
860 870 880 890 900
WWFLYDSTGP AVTYYQLSHF MQCHNHNEDF TGVDCDIFEA SPPMTMALSV
910 920 930 940 950
LVTIEMCNAL NSLSENQSLI RMPPWSNLWL MAAMTLSMSL HFMIIYVDPL
960 970 980 990
PMIFKLTHLT FDQWLMVFKL SFPVILIDEV LKFFARNYIE TGKEVK
Length:996
Mass (Da):109,247
Last modified:December 1, 2000 - v2
Checksum:i821032E8D5F36392
GO
Isoform SERCA1A (identifier: P70083-2) [UniParc]FASTAAdd to basket
Also known as: ATP2A1A

The sequence of this isoform differs from the canonical sequence as follows:
     991-996: TGKEVK → K

Show »
Length:991
Mass (Da):108,733
Checksum:iF1F392AB4A133430
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti577D → Y in AAB08097 (PubMed:11079376).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000357991 – 996TGKEVK → K in isoform SERCA1A. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65229 mRNA. Translation: AAB08098.1.
U65228 mRNA. Translation: AAB08097.1.
U58321 mRNA. Translation: AAB17073.1.
U58327 mRNA. Translation: AAB17075.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65229 mRNA. Translation: AAB08098.1.
U65228 mRNA. Translation: AAB08097.1.
U58321 mRNA. Translation: AAB17073.1.
U58327 mRNA. Translation: AAB17075.1.

3D structure databases

ProteinModelPortaliP70083.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP70083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG105648.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2A1_MAKNI
AccessioniPrimary (citable) accession number: P70083
Secondary accession number(s): P70084, Q91100, Q92082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.