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Protein

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Gene

atp2a1

Organism
Makaira nigricans (Atlantic blue marlin)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Inhibited by sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (By similarity). Enhanced by DWORF; DWORF increases activity by displacing sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi304 – 3041Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi305 – 3051Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi307 – 3071Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi309 – 3091Calcium 2By similarity
Active sitei351 – 35114-aspartylphosphate intermediateBy similarity
Metal bindingi700 – 7001MagnesiumBy similarity
Metal bindingi704 – 7041MagnesiumBy similarity
Metal bindingi765 – 7651Calcium 1By similarity
Metal bindingi768 – 7681Calcium 1By similarity
Metal bindingi793 – 7931Calcium 2By similarity
Metal bindingi796 – 7961Calcium 1By similarity
Metal bindingi797 – 7971Calcium 1By similarity
Metal bindingi797 – 7971Calcium 2By similarity
Metal bindingi905 – 9051Calcium 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC:3.6.3.8)
Short name:
SERCA1
Short name:
SR Ca(2+)-ATPase 1
Alternative name(s):
Calcium pump 1
Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene namesi
Name:atp2a1
OrganismiMakaira nigricans (Atlantic blue marlin)
Taxonomic identifieri13604 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataCarangariaIstiophoriformesMakaira

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848CytoplasmicBy similarityAdd
BLAST
Transmembranei49 – 6921Helical; Name=1By similarityAdd
BLAST
Topological domaini70 – 8920LumenalBy similarityAdd
BLAST
Transmembranei90 – 11021Helical; Name=2By similarityAdd
BLAST
Topological domaini111 – 253143CytoplasmicBy similarityAdd
BLAST
Transmembranei254 – 27320Helical; Name=3By similarityAdd
BLAST
Topological domaini274 – 29522LumenalBy similarityAdd
BLAST
Transmembranei296 – 31318Helical; Name=4By similarityAdd
BLAST
Topological domaini314 – 754441CytoplasmicBy similarityAdd
BLAST
Transmembranei755 – 77420Helical; Name=5By similarityAdd
BLAST
Topological domaini775 – 78410LumenalBy similarity
Transmembranei785 – 80521Helical; Name=6By similarityAdd
BLAST
Topological domaini806 – 82520CytoplasmicBy similarityAdd
BLAST
Transmembranei826 – 84823Helical; Name=7By similarityAdd
BLAST
Topological domaini849 – 89446LumenalBy similarityAdd
BLAST
Transmembranei895 – 91420Helical; Name=8By similarityAdd
BLAST
Topological domaini915 – 92713CytoplasmicBy similarityAdd
BLAST
Transmembranei928 – 94619Helical; Name=9By similarityAdd
BLAST
Topological domaini947 – 96115LumenalBy similarityAdd
BLAST
Transmembranei962 – 98221Helical; Name=10By similarityAdd
BLAST
Topological domaini983 – 99614CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 996996Sarcoplasmic/endoplasmic reticulum calcium ATPase 1PRO_0000046192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi873 ↔ 885By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiP70083.

Interactioni

Subunit structurei

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP70083.
SMRiP70083. Positions 1-990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni370 – 40031Interacts with phospholamban 1By similarityAdd
BLAST
Regioni785 – 80521Interacts with phospholamban 2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105648.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SERCA1B (identifier: P70083-1) [UniParc]FASTAAdd to basket

Also known as: ATP2A1B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENAHTKSPA ECLSYFGVNE HTGLSPDQFK KNLDKFGYNE LPAEEGKSIW
60 70 80 90 100
DLIVEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA
110 120 130 140 150
NAIVGVWQER NAEDAIEALK EYEPEMGKVY RSDRKSVQRI KAREIVPGDI
160 170 180 190 200
VEVSVGDKVP ADIRIVSIKS TTLRVDQSIL TGESVSVIKH TESVPDPRAV
210 220 230 240 250
NQDKKNMLFS GTNIAAGKAI GVAIATGVST EIGKIRDQMA ATEQEKTPLQ
260 270 280 290 300
AKLDEFGEQL SKVISLICVA VWAINIGHFN DPVHGGSWIR GAVYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MCVTKMFIVK SVDGDHVDLN AFDISGSKYT PEGEVSHGGS
410 420 430 440 450
KTNCSAYDGL VELATICALC NDSSLDYNES KKIYEKVGEA TETALCCLVE
460 470 480 490 500
KMNVFNSNVK NLSRIERANA CCTVIKQLMK KNFTLEFSRD RKSMSVYCTP
510 520 530 540 550
AKGDGGAKMF VKGAPEGVID RCAYVRVGTT RVPLTSAIKE KIMAVIRDWG
560 570 580 590 600
TGRDTLRCLA LATRDTPLKV EEMNLEDSTK FADYETDMTF VGCVGMLDPP
610 620 630 640 650
RKEVTGSIEL CRDAGIRVIM ITGDNKGTAI AICRRIGIFK EDEDVSNKAY
660 670 680 690 700
TGREFDDLPS QDQAEAVRRA CCFARVEPSH KSKIVEFLQG NDDITAMTGD
710 720 730 740 750
GVNDAPALKK AEIGIAMGSG TAVAKSASEM VLADDNFSSI VAAVEEGRAI
760 770 780 790 800
YNNMKQFIRY LISSNVGEVV CIFLTAALGL PEALIPVQLL WVNLVTDGLP
810 820 830 840 850
ATALGFNPPD LDIMGKPPRS PKEPLISGWL FFRYMAIGGY VGAATVGGAA
860 870 880 890 900
WWFLYDSTGP AVTYYQLSHF MQCHNHNEDF TGVDCDIFEA SPPMTMALSV
910 920 930 940 950
LVTIEMCNAL NSLSENQSLI RMPPWSNLWL MAAMTLSMSL HFMIIYVDPL
960 970 980 990
PMIFKLTHLT FDQWLMVFKL SFPVILIDEV LKFFARNYIE TGKEVK
Length:996
Mass (Da):109,247
Last modified:December 1, 2000 - v2
Checksum:i821032E8D5F36392
GO
Isoform SERCA1A (identifier: P70083-2) [UniParc]FASTAAdd to basket

Also known as: ATP2A1A

The sequence of this isoform differs from the canonical sequence as follows:
     991-996: TGKEVK → K

Show »
Length:991
Mass (Da):108,733
Checksum:iF1F392AB4A133430
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti577 – 5771D → Y in AAB08097 (PubMed:11079376).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei991 – 9966TGKEVK → K in isoform SERCA1A. 1 PublicationVSP_000357

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65229 mRNA. Translation: AAB08098.1.
U65228 mRNA. Translation: AAB08097.1.
U58321 mRNA. Translation: AAB17073.1.
U58327 mRNA. Translation: AAB17075.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65229 mRNA. Translation: AAB08098.1.
U65228 mRNA. Translation: AAB08097.1.
U58321 mRNA. Translation: AAB17073.1.
U58327 mRNA. Translation: AAB17075.1.

3D structure databases

ProteinModelPortaliP70083.
SMRiP70083. Positions 1-990.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP70083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG105648.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a neonatal calcium atpase isoform (SERCA 1B) from extraocular muscle of adult blue marlin (Makaira nigricans)."
    Londraville R.L., Cramer T.D., Franck J.P.C.F., Tullis A., Block B.A.
    Comp. Biochem. Physiol. 127B:223-233(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SERCA1A AND SERCA1B).
    Tissue: Extraocular muscle.
  2. "Expression of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in marlin and swordfish muscle and heater cells."
    Tullis A., Block B.A.
    Am. J. Physiol. 271:R262-R275(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 349-438 AND 448-578.
    Tissue: Heating tissue and Muscle.

Entry informationi

Entry nameiAT2A1_MAKNI
AccessioniPrimary (citable) accession number: P70083
Secondary accession number(s): P70084, Q91100, Q92082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 13, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.