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Reviewed, UniProtKB/Swiss-Prot P70083 (AT2A1_MAKNI)

Last modified February 9, 2010. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
      Short name=SR Ca(2+)-ATPase 1
      Short name=SERCA1
    EC=3.6.3.8
Alternative name(s):
    Calcium pump 1
    Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
    Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene names
Name: atp2a1
OrganismMakaira nigricans (Blue marlin)
Taxonomic identifier13604 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Scombroidei › Makaira

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Protein attributes

Sequence length996 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction By similarity.

Catalytic activity

ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Enzyme regulation

Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN By similarity.

Subunit structure

Associated with sarcolipin (SLN) and phospholamban (PLN) By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIA subfamily.

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Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
Sarcoplasmic reticulum
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
   LigandATP-binding
Calcium
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMDisulfide bond
Phosphoprotein
Gene Ontology (GO)
   Biological processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

ATP catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of striated muscle contraction

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of fast-twitch skeletal muscle fiber contraction

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentER-Golgi intermediate compartment

Inferred from sequence or structural similarity. Source: UniProtKB

H zone

Inferred from sequence or structural similarity. Source: UniProtKB

I band

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

sarcoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

calcium ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-transporting ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform SERCA1B (identifier: P70083-1)

Also known as: ATP2A1B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform SERCA1A (identifier: P70083-2)

Also known as: ATP2A1A;

The sequence of this isoform differs from the canonical sequence as follows:
     991-996: TGKEVK → K

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 996996Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
PRO_0000046192

Regions

Topological domain1 – 4848Cytoplasmic By similarity
Transmembrane49 – 69211 By similarity
Topological domain70 – 8920Lumenal By similarity
Transmembrane90 – 110212 By similarity
Topological domain111 – 253143Cytoplasmic By similarity
Transmembrane254 – 273203 By similarity
Topological domain274 – 29522Lumenal By similarity
Transmembrane296 – 313184 By similarity
Topological domain314 – 754441Cytoplasmic By similarity
Transmembrane755 – 774205 By similarity
Topological domain775 – 78410Lumenal By similarity
Transmembrane785 – 805216 By similarity
Topological domain806 – 82520Cytoplasmic By similarity
Transmembrane826 – 848237 By similarity
Topological domain849 – 89446Lumenal By similarity
Transmembrane895 – 914208 By similarity
Topological domain915 – 92713Cytoplasmic By similarity
Transmembrane928 – 946199 By similarity
Topological domain947 – 96115Lumenal By similarity
Transmembrane962 – 9822110 By similarity
Topological domain983 – 99614Cytoplasmic By similarity
Region370 – 40031Interacts with phospholamban 1 By similarity
Region785 – 80521Interacts with phospholamban 2 By similarity

Sites

Active site35114-aspartylphosphate intermediate By similarity
Metal binding3041Calcium 2; via carbonyl oxygen By similarity
Metal binding3051Calcium 2; via carbonyl oxygen By similarity
Metal binding3071Calcium 2; via carbonyl oxygen By similarity
Metal binding3091Calcium 2 By similarity
Metal binding7001Magnesium By similarity
Metal binding7041Magnesium By similarity
Metal binding7651Calcium 1 By similarity
Metal binding7681Calcium 1 By similarity
Metal binding7931Calcium 2 By similarity
Metal binding7961Calcium 1 By similarity
Metal binding7971Calcium 1 By similarity
Metal binding7971Calcium 2 By similarity
Metal binding9051Calcium 1 By similarity

Amino acid modifications

Disulfide bond873 ↔ 885 By similarity

Natural variations

Alternative sequence991 – 9966TGKEVK → K in isoform SERCA1A.
VSP_000357

Experimental info

Sequence conflict5771D → Y in AAB08097. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform SERCA1B (ATP2A1B) [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 821032E8D5F36392

FASTA996109,247
        10         20         30         40         50         60 
MENAHTKSPA ECLSYFGVNE HTGLSPDQFK KNLDKFGYNE LPAEEGKSIW DLIVEQFEDL 

        70         80         90        100        110        120 
LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA NAIVGVWQER NAEDAIEALK 

       130        140        150        160        170        180 
EYEPEMGKVY RSDRKSVQRI KAREIVPGDI VEVSVGDKVP ADIRIVSIKS TTLRVDQSIL 

       190        200        210        220        230        240 
TGESVSVIKH TESVPDPRAV NQDKKNMLFS GTNIAAGKAI GVAIATGVST EIGKIRDQMA 

       250        260        270        280        290        300 
ATEQEKTPLQ AKLDEFGEQL SKVISLICVA VWAINIGHFN DPVHGGSWIR GAVYYFKIAV 

       310        320        330        340        350        360 
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 

       370        380        390        400        410        420 
MCVTKMFIVK SVDGDHVDLN AFDISGSKYT PEGEVSHGGS KTNCSAYDGL VELATICALC 

       430        440        450        460        470        480 
NDSSLDYNES KKIYEKVGEA TETALCCLVE KMNVFNSNVK NLSRIERANA CCTVIKQLMK 

       490        500        510        520        530        540 
KNFTLEFSRD RKSMSVYCTP AKGDGGAKMF VKGAPEGVID RCAYVRVGTT RVPLTSAIKE 

       550        560        570        580        590        600 
KIMAVIRDWG TGRDTLRCLA LATRDTPLKV EEMNLEDSTK FADYETDMTF VGCVGMLDPP 

       610        620        630        640        650        660 
RKEVTGSIEL CRDAGIRVIM ITGDNKGTAI AICRRIGIFK EDEDVSNKAY TGREFDDLPS 

       670        680        690        700        710        720 
QDQAEAVRRA CCFARVEPSH KSKIVEFLQG NDDITAMTGD GVNDAPALKK AEIGIAMGSG 

       730        740        750        760        770        780 
TAVAKSASEM VLADDNFSSI VAAVEEGRAI YNNMKQFIRY LISSNVGEVV CIFLTAALGL 

       790        800        810        820        830        840 
PEALIPVQLL WVNLVTDGLP ATALGFNPPD LDIMGKPPRS PKEPLISGWL FFRYMAIGGY 

       850        860        870        880        890        900 
VGAATVGGAA WWFLYDSTGP AVTYYQLSHF MQCHNHNEDF TGVDCDIFEA SPPMTMALSV 

       910        920        930        940        950        960 
LVTIEMCNAL NSLSENQSLI RMPPWSNLWL MAAMTLSMSL HFMIIYVDPL PMIFKLTHLT 

       970        980        990 
FDQWLMVFKL SFPVILIDEV LKFFARNYIE TGKEVK

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Isoform SERCA1A (ATP2A1A).

Checksum: F1F392AB4A133430
Show »

FASTA991108,733

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References

[1]"Cloning of a neonatal calcium atpase isoform (SERCA 1B) from extraocular muscle of adult blue marlin (Makaira nigricans)."
Londraville R.L., Cramer T.D., Franck J.P.C.F., Tullis A., Block B.A.
Comp. Biochem. Physiol. 127B:223-233(2000) [PubMed: 11079376] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SERCA1A AND SERCA1B).
Tissue: Extraocular muscle.
[2]"Expression of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in marlin and swordfish muscle and heater cells."
Tullis A., Block B.A.
Am. J. Physiol. 271:R262-R275(1996) [PubMed: 8760229] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 349-438 AND 448-578.
Tissue: Heating tissue and Muscle.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U65229 mRNA. Translation: AAB08098.1.
U65228 mRNA. Translation: AAB08097.1.
U58321 mRNA. Translation: AAB17073.1.
U58327 mRNA. Translation: AAB17075.1.

3D structure databases

SMRP70083. Positions 1-990.
ModBaseSearch...

Phylogenomic databases

HOVERGENP70083.

Enzyme and pathway databases

BRENDA3.6.3.8. 313791.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR005782. ATPase_P-typ_Ca-transp.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAT2A1_MAKNI
AccessionPrimary (citable) accession number: P70083
Secondary accession number(s): P70084, Q91100, Q92082
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 9, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents