Reviewed, UniProtKB/Swiss-Prot P70080 (TPH1_CHICK)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 1
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 1

Gene names

Name:	TPH1
Synonyms:	TPH

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Multimer of identical subunits By similarity.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process from tryptophan Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Tryptophan 5-hydroxylase 1		PRO_0000205572
Regions
	Domain	18 – 92	75	ACT
Sites
	Metal binding	273	1	Iron By similarity
	Metal binding	278	1	Iron By similarity
	Metal binding	318	1	Iron By similarity
Amino acid modifications
	Modified residue	58	1	Phosphoserine; by PKA Potential

Sequences

	Sequence		Length	Mass (Da)
	P70080-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: FF0041D7C4B159F6	FASTA	445	51,139
	10 20 30 40 50 60 MIEDNKENKD HAPERGRTAI IFSLKNEVGG LVKALKLFQE KHVNLVHIES RKSKRRNSEF 70 80 90 100 110 120 EIFVDCDSNR EQLNEIFQLL KSHVSIVSMN PTEHFNVQED GDMENIPWYP KKISDLDKCA 130 140 150 160 170 180 NRVLMYGSDL DADHPGFKDN VYRKRRKYFA DLAMNYKHGD PIPEIEFTEE EIKTWGTVYR 190 200 210 220 230 240 ELNKLYPTHA CREYLKNLPL LTKYCGYRED NIPQLEDVSR FLKERTGFTI RPVAGYLSPR 250 260 270 280 290 300 DFLAGLAFRV FHCTQYVRHS SDPLYTPEPD TCHELLGHVP LLAEPSFAQF SQEIGLASLG 310 320 330 340 350 360 ASDEAVQKLA TCYFFTVEFG LCKQEGQLRV YGAGLLSSIS ELKHSLSGSA KVKPFDPKVT 370 380 390 400 410 420 CKQECLITTF QEVYFVSESF EEAKEKMREF AKTIKRPFGV KYNPYTQSVQ ILKDTKSIAS 430 440 VVNELRHELD IVSDALSKMG KQLEV

References

[1]	"Molecular cloning of chick pineal tryptophan hydroxylase and circadian oscillation of its mRNA levels." Florez J.C., Seidenman K.J., Barrett R.K., Sangoram A.M., Takahashi J.S. Brain Res. Mol. Brain Res. 42:25-30(1996) [PubMed: 8915576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Pineal gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U26428 mRNA. Translation: AAC60036.1.

IPI

IPI00574058.

RefSeq

NP_990287.1.

UniGene

Gga.43354

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3E2T	X-ray	1.90	A	101-414	[»]

SMR

P70080. Positions 105-394.

ModBase

Search...

Genome annotation databases

Ensembl

ENSGALG00000006236. Gallus gallus. [Contig view]

GeneID

395799.

KEGG

gga:395799.

Phylogenomic databases

HOGENOM

P70080.

HOVERGEN

P70080.

OMA

P70080. TWGTVFQ.

Enzyme and pathway databases

BRENDA

1.14.16.4. 4.

Family and domain databases

InterPro

IPR002912. ACT_bd.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]

Gene3D

G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.

PANTHER

PTHR11473. Aaa_hydroxylase. 1 hit.

Pfam

PF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]

PIRSF

PIRSF000336. TH. 1 hit.

PRINTS

PR00372. FYWHYDRXLASE.

ProDom

PD002559. Aaa_hydroxylase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01270. Trp_5_monoox. 1 hit.

PROSITE

PS00367. BIOPTERIN_HYDROXYL. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPH1_CHICK

Accession

Primary (citable) accession number: P70080

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)