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Protein

Tryptophan 5-hydroxylase 1

Gene

TPH1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Pathwayi: serotonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes serotonin from L-tryptophan.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 5-hydroxylase 1 (TPH1)
  2. no protein annotated in this organism
This subpathway is part of the pathway serotonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes serotonin from L-tryptophan, the pathway serotonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei236 – 2361Tryptophan1 Publication
Binding sitei258 – 2581Tryptophan1 Publication
Binding sitei266 – 2661Tryptophan1 Publication
Metal bindingi273 – 2731Iron
Metal bindingi278 – 2781Iron
Metal bindingi318 – 3181Iron
Binding sitei337 – 3371Tryptophan1 Publication
Binding sitei367 – 3671Tryptophan; via carbonyl oxygen1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.16.4. 1306.
ReactomeiR-GGA-209931. Serotonin and melatonin biosynthesis.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
Alternative name(s):
Tryptophan 5-monooxygenase 1
Gene namesi
Name:TPH1
Synonyms:TPH
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Tryptophan 5-hydroxylase 1PRO_0000205572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine; by PKASequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP70080.

Expressioni

Gene expression databases

BgeeiENSGALG00000006236.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010070.

Structurei

Secondary structure

1
445
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi113 – 1175Combined sources
Turni126 – 1283Combined sources
Helixi135 – 1384Combined sources
Helixi140 – 15516Combined sources
Helixi169 – 18921Combined sources
Helixi192 – 1965Combined sources
Helixi198 – 2058Combined sources
Helixi215 – 22612Combined sources
Beta strandi229 – 2324Combined sources
Beta strandi234 – 2374Combined sources
Helixi239 – 2479Combined sources
Beta strandi250 – 2534Combined sources
Helixi271 – 2777Combined sources
Helixi279 – 2824Combined sources
Helixi285 – 29814Combined sources
Helixi303 – 31412Combined sources
Turni315 – 3195Combined sources
Beta strandi321 – 3244Combined sources
Beta strandi327 – 3304Combined sources
Helixi333 – 3364Combined sources
Helixi339 – 3457Combined sources
Beta strandi352 – 3543Combined sources
Helixi357 – 3604Combined sources
Beta strandi367 – 3693Combined sources
Beta strandi374 – 3796Combined sources
Helixi380 – 39112Combined sources
Beta strandi401 – 4033Combined sources
Turni404 – 4074Combined sources
Beta strandi408 – 4103Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E2TX-ray1.90A101-414[»]
ProteinModelPortaliP70080.
SMRiP70080. Positions 105-439.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70080.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9476ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP70080.
KOiK00502.
OMAiQHAITAE.
OrthoDBiEOG091G05MZ.
PhylomeDBiP70080.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P70080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEDNKENKD HAPERGRTAI IFSLKNEVGG LVKALKLFQE KHVNLVHIES
60 70 80 90 100
RKSKRRNSEF EIFVDCDSNR EQLNEIFQLL KSHVSIVSMN PTEHFNVQED
110 120 130 140 150
GDMENIPWYP KKISDLDKCA NRVLMYGSDL DADHPGFKDN VYRKRRKYFA
160 170 180 190 200
DLAMNYKHGD PIPEIEFTEE EIKTWGTVYR ELNKLYPTHA CREYLKNLPL
210 220 230 240 250
LTKYCGYRED NIPQLEDVSR FLKERTGFTI RPVAGYLSPR DFLAGLAFRV
260 270 280 290 300
FHCTQYVRHS SDPLYTPEPD TCHELLGHVP LLAEPSFAQF SQEIGLASLG
310 320 330 340 350
ASDEAVQKLA TCYFFTVEFG LCKQEGQLRV YGAGLLSSIS ELKHSLSGSA
360 370 380 390 400
KVKPFDPKVT CKQECLITTF QEVYFVSESF EEAKEKMREF AKTIKRPFGV
410 420 430 440
KYNPYTQSVQ ILKDTKSIAS VVNELRHELD IVSDALSKMG KQLEV
Length:445
Mass (Da):51,139
Last modified:February 1, 1997 - v1
Checksum:iFF0041D7C4B159F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26428 mRNA. Translation: AAC60036.1.
RefSeqiNP_990287.1. NM_204956.1.
UniGeneiGga.43354.

Genome annotation databases

EnsembliENSGALT00000010084; ENSGALP00000010070; ENSGALG00000006236.
GeneIDi395799.
KEGGigga:395799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26428 mRNA. Translation: AAC60036.1.
RefSeqiNP_990287.1. NM_204956.1.
UniGeneiGga.43354.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E2TX-ray1.90A101-414[»]
ProteinModelPortaliP70080.
SMRiP70080. Positions 105-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010070.

Proteomic databases

PaxDbiP70080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000010084; ENSGALP00000010070; ENSGALG00000006236.
GeneIDi395799.
KEGGigga:395799.

Organism-specific databases

CTDi7166.

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP70080.
KOiK00502.
OMAiQHAITAE.
OrthoDBiEOG091G05MZ.
PhylomeDBiP70080.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00846; UER00799.
BRENDAi1.14.16.4. 1306.
ReactomeiR-GGA-209931. Serotonin and melatonin biosynthesis.

Miscellaneous databases

EvolutionaryTraceiP70080.
PROiP70080.

Gene expression databases

BgeeiENSGALG00000006236.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPH1_CHICK
AccessioniPrimary (citable) accession number: P70080
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.