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P70079

- KCRU_CHICK

UniProt

P70079 - KCRU_CHICK

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Protein

Creatine kinase U-type, mitochondrial

Gene

CKMT1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241ATPPROSITE-ProRule annotation
Binding sitei269 – 2691ATPPROSITE-ProRule annotation
Binding sitei325 – 3251ATPPROSITE-ProRule annotation
Binding sitei368 – 3681ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi161 – 1655ATPPROSITE-ProRule annotation
Nucleotide bindingi353 – 3586ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. creatine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name:
Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name:
U-MtCK
Gene namesi
Name:CKMT1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
BLAST
Chaini40 – 417378Creatine kinase U-type, mitochondrialPRO_0000016593Add
BLAST

Proteomic databases

PaxDbiP70079.
PRIDEiP70079.

Expressioni

Tissue specificityi

Highly expressed in brain and gut, weakly in testis. Also found in cell bodies of neural cells of the gray matter.

Interactioni

Subunit structurei

Exists as an octamer composed of four MTCK homodimers.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013585.

Structurei

3D structure databases

ProteinModelPortaliP70079.
SMRiP70079. Positions 39-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 13188Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini158 – 400243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3869.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP70079.
KOiK00933.
PhylomeDBiP70079.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70079-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTFARALS ARRSAGLLAM VGAGSLAAGF LLARDTVSAG ERQRRRYPPS
60 70 80 90 100
AEYPDLRKHN NCMASNLTPA IYARLCDKAT PNGWTLDQCI QTGVDNPGHP
110 120 130 140 150
FIKTVGMVAG DEETYEVFAE LFDPVIQERH NGYNPRTMKH VTDLDASKIK
160 170 180 190 200
FGHFDERYVL SSRVRTGRSI RGLSLPPACT RAERREVEKV TVEALNGLTG
210 220 230 240 250
DLSGRYYRLS EMTEKEQQQL IDDHFLFDKP VSPSLTAAGM ARDWPDARGI
260 270 280 290 300
WHNNEKTFLI WINEEDHTRV ISMEKGGNMK RVFERFCRGL KEVERLIQER
310 320 330 340 350
GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL
360 370 380 390 400
QKRGTGGVDT AATANVFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL
410
EKGQDIRIPS PVPQFRH
Length:417
Mass (Da):47,104
Last modified:February 1, 1997 - v1
Checksum:iED9B982764153D2C
GO

Sequence cautioni

The sequence CAA65266.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96403 Genomic DNA. Translation: CAA65267.1.
X96402 Genomic DNA. Translation: CAA65266.1. Different initiation.
X95526 Genomic DNA. Translation: CAA64778.1.
RefSeqiNP_989513.1. NM_204182.1.
UniGeneiGga.13490.

Genome annotation databases

GeneIDi374002.
KEGGigga:374002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96403 Genomic DNA. Translation: CAA65267.1 .
X96402 Genomic DNA. Translation: CAA65266.1 . Different initiation.
X95526 Genomic DNA. Translation: CAA64778.1 .
RefSeqi NP_989513.1. NM_204182.1.
UniGenei Gga.13490.

3D structure databases

ProteinModelPortali P70079.
SMRi P70079. Positions 39-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000013585.

Proteomic databases

PaxDbi P70079.
PRIDEi P70079.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 374002.
KEGGi gga:374002.

Organism-specific databases

CTDi 548596.

Phylogenomic databases

eggNOGi COG3869.
HOGENOMi HOG000232165.
HOVERGENi HBG001339.
InParanoidi P70079.
KOi K00933.
PhylomeDBi P70079.

Miscellaneous databases

NextBioi 20813530.

Family and domain databases

Gene3Di 1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view ]
Pfami PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view ]
SUPFAMi SSF48034. SSF48034. 1 hit.
PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution of the creative kinases. The chicken acidic type mitochondrial creatine kinase gene as the first nonmammalian gene."
    Muehlebach S.M., Wirz T., Braendle U., Perriard J.-C.
    J. Biol. Chem. 271:11920-11929(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: White leghorn.
    Tissue: Brain and Liver.
  2. "Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework."
    Hossle J.P., Schlegel J., Wegmann G., Wyss M., Boehlen P., Eppenberger H.M., Wallimann T., Perriard J.-C.
    Biochem. Biophys. Res. Commun. 151:408-416(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 40-68.
    Tissue: Brain.

Entry informationi

Entry nameiKCRU_CHICK
AccessioniPrimary (citable) accession number: P70079
Secondary accession number(s): Q90782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3