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P70079

- KCRU_CHICK

UniProt

P70079 - KCRU_CHICK

Protein

Creatine kinase U-type, mitochondrial

Gene

CKMT1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei224 – 2241ATPPROSITE-ProRule annotation
    Binding sitei269 – 2691ATPPROSITE-ProRule annotation
    Binding sitei325 – 3251ATPPROSITE-ProRule annotation
    Binding sitei368 – 3681ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi161 – 1655ATPPROSITE-ProRule annotation
    Nucleotide bindingi353 – 3586ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
    Alternative name(s):
    Acidic-type mitochondrial creatine kinase
    Short name:
    Mia-CK
    Ubiquitous mitochondrial creatine kinase
    Short name:
    U-MtCK
    Gene namesi
    Name:CKMT1
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
    BLAST
    Chaini40 – 417378Creatine kinase U-type, mitochondrialPRO_0000016593Add
    BLAST

    Proteomic databases

    PaxDbiP70079.
    PRIDEiP70079.

    Expressioni

    Tissue specificityi

    Highly expressed in brain and gut, weakly in testis. Also found in cell bodies of neural cells of the gray matter.

    Interactioni

    Subunit structurei

    Exists as an octamer composed of four MTCK homodimers.By similarity

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000013585.

    Structurei

    3D structure databases

    ProteinModelPortaliP70079.
    SMRiP70079. Positions 39-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 13188Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini158 – 400243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3869.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.
    InParanoidiP70079.
    KOiK00933.
    PhylomeDBiP70079.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P70079-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTFARALS ARRSAGLLAM VGAGSLAAGF LLARDTVSAG ERQRRRYPPS    50
    AEYPDLRKHN NCMASNLTPA IYARLCDKAT PNGWTLDQCI QTGVDNPGHP 100
    FIKTVGMVAG DEETYEVFAE LFDPVIQERH NGYNPRTMKH VTDLDASKIK 150
    FGHFDERYVL SSRVRTGRSI RGLSLPPACT RAERREVEKV TVEALNGLTG 200
    DLSGRYYRLS EMTEKEQQQL IDDHFLFDKP VSPSLTAAGM ARDWPDARGI 250
    WHNNEKTFLI WINEEDHTRV ISMEKGGNMK RVFERFCRGL KEVERLIQER 300
    GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL 350
    QKRGTGGVDT AATANVFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL 400
    EKGQDIRIPS PVPQFRH 417
    Length:417
    Mass (Da):47,104
    Last modified:February 1, 1997 - v1
    Checksum:iED9B982764153D2C
    GO

    Sequence cautioni

    The sequence CAA65266.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96403 Genomic DNA. Translation: CAA65267.1.
    X96402 Genomic DNA. Translation: CAA65266.1. Different initiation.
    X95526 Genomic DNA. Translation: CAA64778.1.
    RefSeqiNP_989513.1. NM_204182.1.
    UniGeneiGga.13490.

    Genome annotation databases

    GeneIDi374002.
    KEGGigga:374002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96403 Genomic DNA. Translation: CAA65267.1 .
    X96402 Genomic DNA. Translation: CAA65266.1 . Different initiation.
    X95526 Genomic DNA. Translation: CAA64778.1 .
    RefSeqi NP_989513.1. NM_204182.1.
    UniGenei Gga.13490.

    3D structure databases

    ProteinModelPortali P70079.
    SMRi P70079. Positions 39-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000013585.

    Proteomic databases

    PaxDbi P70079.
    PRIDEi P70079.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 374002.
    KEGGi gga:374002.

    Organism-specific databases

    CTDi 548596.

    Phylogenomic databases

    eggNOGi COG3869.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.
    InParanoidi P70079.
    KOi K00933.
    PhylomeDBi P70079.

    Miscellaneous databases

    NextBioi 20813530.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of the creative kinases. The chicken acidic type mitochondrial creatine kinase gene as the first nonmammalian gene."
      Muehlebach S.M., Wirz T., Braendle U., Perriard J.-C.
      J. Biol. Chem. 271:11920-11929(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: White leghorn.
      Tissue: Brain and Liver.
    2. "Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework."
      Hossle J.P., Schlegel J., Wegmann G., Wyss M., Boehlen P., Eppenberger H.M., Wallimann T., Perriard J.-C.
      Biochem. Biophys. Res. Commun. 151:408-416(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-68.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCRU_CHICK
    AccessioniPrimary (citable) accession number: P70079
    Secondary accession number(s): Q90782
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mitochondrial creatine kinase binds cardiolipin.By similarity

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3