Reviewed, UniProtKB/Swiss-Prot P70079 (KCRU_CHICK)

Names and origin

Protein names

Recommended name:
    Creatine kinase, ubiquitous mitochondrial
    EC=2.7.3.2
Alternative name(s):
    U-MtCK
    Acidic-type mitochondrial creatine kinase
      Short name=Mia-CK

Gene names

Name:

CKMT1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four MTCK homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Tissue specificity	Highly expressed in brain and gut, weakly in testis. Also found in cell bodies of neural cells of the gray matter.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin By similarity.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion
	Chain	40 – 417	378	Creatine kinase, ubiquitous mitochondrial		PRO_0000016593
Regions
	Nucleotide binding	161 – 165	5	ATP By similarity
	Nucleotide binding	353 – 358	6	ATP By similarity
Sites
	Binding site	224	1	ATP By similarity
	Binding site	269	1	ATP By similarity
	Binding site	325	1	ATP By similarity
	Binding site	368	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70079-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: ED9B982764153D2C	FASTA	417	47,104
	10 20 30 40 50 60 MASTFARALS ARRSAGLLAM VGAGSLAAGF LLARDTVSAG ERQRRRYPPS AEYPDLRKHN 70 80 90 100 110 120 NCMASNLTPA IYARLCDKAT PNGWTLDQCI QTGVDNPGHP FIKTVGMVAG DEETYEVFAE 130 140 150 160 170 180 LFDPVIQERH NGYNPRTMKH VTDLDASKIK FGHFDERYVL SSRVRTGRSI RGLSLPPACT 190 200 210 220 230 240 RAERREVEKV TVEALNGLTG DLSGRYYRLS EMTEKEQQQL IDDHFLFDKP VSPSLTAAGM 250 260 270 280 290 300 ARDWPDARGI WHNNEKTFLI WINEEDHTRV ISMEKGGNMK RVFERFCRGL KEVERLIQER 310 320 330 340 350 360 GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL QKRGTGGVDT 370 380 390 400 410 AATANVFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL EKGQDIRIPS PVPQFRH

References

[1]	"Evolution of the creative kinases. The chicken acidic type mitochondrial creatine kinase gene as the first nonmammalian gene." Muehlebach S.M., Wirz T., Braendle U., Perriard J.-C. J. Biol. Chem. 271:11920-11929(1996) [PubMed: 8662608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: White leghorn. Tissue: Brain and Liver.
[2]	"Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework." Hossle J.P., Schlegel J., Wegmann G., Wyss M., Boehlen P., Eppenberger H.M., Wallimann T., Perriard J.-C. Biochem. Biophys. Res. Commun. 151:408-416(1988) [PubMed: 2831887] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-68. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96403 Genomic DNA. Translation: CAA65267.1. X96402 Genomic DNA. Translation: CAA65266.1. Different initiation. X95526 Genomic DNA. Translation: CAA64778.1.
RefSeq	NP_989513.1.
UniGene	Gga.13490
3D structure databases
HSSP	HSSP built from PDB template 1QK1 based on UniProtKB P12532.
SMR	P70079. Positions 47-412.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000008352. Gallus gallus. [Contig view]
GeneID	374002.
KEGG	gga:374002.
Phylogenomic databases
HOGENOM	P70079.
HOVERGEN	P70079.
Family and domain databases
InterPro	IPR000749. ATP-gua_Ptrans. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRU_CHICK

Accession

Primary (citable) accession number: P70079
Secondary accession number(s): Q90782

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)