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P70079 (KCRU_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Creatine kinase U-type, mitochondrial

EC=2.7.3.2
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name=Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name=U-MtCK
Gene names
Name:CKMT1
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four MTCK homodimers By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Tissue specificity

Highly expressed in brain and gut, weakly in testis. Also found in cell bodies of neural cells of the gray matter.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin By similarity.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Sequence caution

The sequence CAA65266.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion Ref.2
Chain40 – 417378Creatine kinase U-type, mitochondrial
PRO_0000016593

Regions

Domain44 – 13188Phosphagen kinase N-terminal
Domain158 – 400243Phosphagen kinase C-terminal
Nucleotide binding161 – 1655ATP By similarity
Nucleotide binding353 – 3586ATP By similarity

Sites

Binding site2241ATP By similarity
Binding site2691ATP By similarity
Binding site3251ATP By similarity
Binding site3681ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P70079 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: ED9B982764153D2C

FASTA41747,104
        10         20         30         40         50         60 
MASTFARALS ARRSAGLLAM VGAGSLAAGF LLARDTVSAG ERQRRRYPPS AEYPDLRKHN 

        70         80         90        100        110        120 
NCMASNLTPA IYARLCDKAT PNGWTLDQCI QTGVDNPGHP FIKTVGMVAG DEETYEVFAE 

       130        140        150        160        170        180 
LFDPVIQERH NGYNPRTMKH VTDLDASKIK FGHFDERYVL SSRVRTGRSI RGLSLPPACT 

       190        200        210        220        230        240 
RAERREVEKV TVEALNGLTG DLSGRYYRLS EMTEKEQQQL IDDHFLFDKP VSPSLTAAGM 

       250        260        270        280        290        300 
ARDWPDARGI WHNNEKTFLI WINEEDHTRV ISMEKGGNMK RVFERFCRGL KEVERLIQER 

       310        320        330        340        350        360 
GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL QKRGTGGVDT 

       370        380        390        400        410 
AATANVFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL EKGQDIRIPS PVPQFRH 

« Hide

References

[1]"Evolution of the creative kinases. The chicken acidic type mitochondrial creatine kinase gene as the first nonmammalian gene."
Muehlebach S.M., Wirz T., Braendle U., Perriard J.-C.
J. Biol. Chem. 271:11920-11929(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: White leghorn.
Tissue: Brain and Liver.
[2]"Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework."
Hossle J.P., Schlegel J., Wegmann G., Wyss M., Boehlen P., Eppenberger H.M., Wallimann T., Perriard J.-C.
Biochem. Biophys. Res. Commun. 151:408-416(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-68.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96403 Genomic DNA. Translation: CAA65267.1.
X96402 Genomic DNA. Translation: CAA65266.1. Different initiation.
X95526 Genomic DNA. Translation: CAA64778.1.
RefSeqNP_989513.1. NM_204182.1.
UniGeneGga.13490.

3D structure databases

ProteinModelPortalP70079.
SMRP70079. Positions 39-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000013585.

Proteomic databases

PaxDbP70079.
PRIDEP70079.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID374002.
KEGGgga:374002.

Organism-specific databases

CTD548596.

Phylogenomic databases

eggNOGCOG3869.
HOGENOMHOG000232165.
HOVERGENHBG001339.
InParanoidP70079.
KOK00933.
PhylomeDBP70079.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20813530.

Entry information

Entry nameKCRU_CHICK
AccessionPrimary (citable) accession number: P70079
Secondary accession number(s): Q90782
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families