P70059 (TRY2_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin EC=3.4.21.4
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	By similarity
	Propeptide	16 – 21	6	Activation peptide By similarity		PRO_0000028235
	Chain	22 – 244	223	Trypsin		PRO_0000028236
Regions
	Domain	22 – 242	221	Peptidase S1
Sites
	Active site	61	1	Charge relay system By similarity
	Active site	105	1	Charge relay system By similarity
	Active site	198	1	Charge relay system By similarity
	Metal binding	73	1	Calcium By similarity
	Metal binding	75	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	83	1	Calcium By similarity
	Site	192	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	28 ↔ 158		By similarity
	Disulfide bond	46 ↔ 62		By similarity
	Disulfide bond	130 ↔ 231		By similarity
	Disulfide bond	137 ↔ 204		By similarity
	Disulfide bond	169 ↔ 183		By similarity
	Disulfide bond	194 ↔ 218		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P70059 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C63F29CB3300B323	FASTA	244	26,080
	10 20 30 40 50 60 MKFLVILVLL GAAVAFEDDD KIVGGFTCAK NAVPYQVSLN AGYHFCGGSL INSQWVVSAA 70 80 90 100 110 120 HCYKSRIQVR LGEHNIALNE GTEQFIDSQK VIKHPNYNSR NLDNDIMLIK LSTTARLSAN 130 140 150 160 170 180 IQSVPLPSAC ASAGTNCLIS GWGNTLSSGT NYPDLLQCLN APILTDSQCS NSYPGEITKN 190 200 210 220 230 240 MFCAGFLAGG KDSCQGDSGG PVVCNGQLQG VVSWGYGCAQ RNYPGVYTKV CNFVTWIQST ISSN

References

[1]	Wang K., Lytle L., Gan L., Hood L.E. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72330 mRNA. Translation: AAB17274.1.
UniGene	Xl.1858.
3D structure databases
ProteinModelPortal	P70059.
SMR	P70059. Positions 22-244.
ModBase	Search...
Protein family/group databases
MEROPS	S01.126.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Xenbase	XB-GENE-5776767. prss1.
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY2_XENLA

Accession

Primary (citable) accession number: P70059

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	March 6, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program